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ANADOLU UNIVERSITY JOURNAL OF SCIENCE AND TECHNOLOGY –C Life Sciences and Biotechnology最新文献

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ATTRACTIVE APPROACHES in DRUG DELIVERY: BIO-BASED CARRIERS for siRNA TARGETING 有吸引力的药物递送方法:siRNA靶向的生物基载体
ANADOLU UNIVERSITY JOURNAL OF SCIENCE AND TECHNOLOGY –C Life Sciences and Biotechnology Pub Date : 2018-02-28 DOI: 10.18036/aubtdc.315579
Zulal Yalinca, S. Tüzmen
{"title":"ATTRACTIVE APPROACHES in DRUG DELIVERY: BIO-BASED CARRIERS for siRNA TARGETING","authors":"Zulal Yalinca, S. Tüzmen","doi":"10.18036/aubtdc.315579","DOIUrl":"https://doi.org/10.18036/aubtdc.315579","url":null,"abstract":"Functional characterization of genes can be determined by disruption of gene expression. This provides powerful approach in designing novel treatment strategies. RNA interference (RNAi) is a natural phenomenon that can aid in the study of post-transcriptional regulation of genes. This mechanism can be stimulated via introduction of double stranded RNA (dsRNA) in a cell. Synthetic short/small interfering RNAs (siRNA) can be utilized to trigger down-regulation of desired genes via transfecting into mammalian cells. Recently, utilization of bio-based polymers has been an attractive approach in drug delivery for medical applications. This review article describes the advantages of exploiting bio-based polymeric carriers for siRNA targeting, as bio-therapeutics. Here, we report the current developments, safety and delivery of bio-based siRNAs via polymeric carriers. Additionally, cancer genetics and metabolic disorders including obesity and diabetes pertaining to the progress in clinical applications have been highlighted.","PeriodicalId":7738,"journal":{"name":"ANADOLU UNIVERSITY JOURNAL OF SCIENCE AND TECHNOLOGY –C Life Sciences and Biotechnology","volume":"7 1","pages":"74-89"},"PeriodicalIF":0.0,"publicationDate":"2018-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42028254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Parasporins as a natural anti-cancer protein 副通道蛋白作为一种天然抗癌蛋白
ANADOLU UNIVERSITY JOURNAL OF SCIENCE AND TECHNOLOGY –C Life Sciences and Biotechnology Pub Date : 2017-08-31 DOI: 10.18036/AUBTDC.307261
M. Oktay, H. Güneş
{"title":"Parasporins as a natural anti-cancer protein","authors":"M. Oktay, H. Güneş","doi":"10.18036/AUBTDC.307261","DOIUrl":"https://doi.org/10.18036/AUBTDC.307261","url":null,"abstract":"Bacillus thuringiensis dogada genis yayilim gosteren, gram pozitif ve spor olusturan bir bakteridir. Sporulasyon evresinde, sporlarla birlikte bircok bocek cesidine karsi insektisit etki gosteren kristal proteinleri de uretir. Insektisidal kristal proteinler Cry ve Cyt proteinler olup; aktivitelerini duyarli boceklerin orta barsak membranlarinda bulunan reseptore baglanarak gosterirler. Bu reseptor baglantili ozgul aktivitelerinden dolayi zirai mucadelede onemlidirler. Daha sonralari, insektisit olanlara gore dogada daha genis dagilim gosteren insektisit olmayan kristal proteinler uzerindeki calismalar yeni bir protein sinifi olan parasporinleri ortaya cikarmistir. Parasporinler ayricalikli olarak kanser hucrelerine karsi sitotoksik etki gosterirken normal hucrelere etki etmez ve hemolitik etki gostermez. Şimdiye kadar alti parasporin ailesi tanimlanmis olup toplamda 19 alt grup bilinmektedir. Parasporinler de insektisidal kristal proteinlere benzer sekilde reseptor baglantili etki gosterdiginden kanser tedavisiyle ilgili calismalarda yeni bir arastirma alaninin ortaya cikmasini saglamistir. Bu derlemede; parasporinlerin kesfi, ozellikleri, cesitleri ve etki mekanizmalari hakkinda yapilmis olan calismalar ozetle anlatilmistir.","PeriodicalId":7738,"journal":{"name":"ANADOLU UNIVERSITY JOURNAL OF SCIENCE AND TECHNOLOGY –C Life Sciences and Biotechnology","volume":"6 1","pages":"103-113"},"PeriodicalIF":0.0,"publicationDate":"2017-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43988673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
THE HERPETOLOGICAL COLLECTION OF ZOOLOGY MUSEUM, ISTANBUL UNIVERSITY 伊斯坦布尔大学动物博物馆爬虫类收藏
ANADOLU UNIVERSITY JOURNAL OF SCIENCE AND TECHNOLOGY –C Life Sciences and Biotechnology Pub Date : 2017-08-31 DOI: 10.18036/AUBTDC.286684
Nilgün Kaya, Oya Özuluğ
{"title":"THE HERPETOLOGICAL COLLECTION OF ZOOLOGY MUSEUM, ISTANBUL UNIVERSITY","authors":"Nilgün Kaya, Oya Özuluğ","doi":"10.18036/AUBTDC.286684","DOIUrl":"https://doi.org/10.18036/AUBTDC.286684","url":null,"abstract":"Amphibia and Reptilia collections at the Zoological Museum, University of Istanbul (ZMUI) were studied and revised. The samples were collected from 1921 until today. The localities of most species are found in the diffrent regions of Turkey. The collection have 74 species of which 20 species are amphibia and 54 species are reptiles.","PeriodicalId":7738,"journal":{"name":"ANADOLU UNIVERSITY JOURNAL OF SCIENCE AND TECHNOLOGY –C Life Sciences and Biotechnology","volume":"6 1","pages":"55-63"},"PeriodicalIF":0.0,"publicationDate":"2017-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44270922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
ENZYMATIC AND ANTIBIOTIC SUSCEPTIBILITY ACTIVITIES OF PHENOL DEGRADING BACTERIA ISOLATED FROM WASTE WATER 废水中苯酚降解菌的酶活性和抗生素敏感性研究
ANADOLU UNIVERSITY JOURNAL OF SCIENCE AND TECHNOLOGY –C Life Sciences and Biotechnology Pub Date : 2017-08-31 DOI: 10.18036/AUBTDC.289555
Nilgün Poyraz, M. Mutlu
{"title":"ENZYMATIC AND ANTIBIOTIC SUSCEPTIBILITY ACTIVITIES OF PHENOL DEGRADING BACTERIA ISOLATED FROM WASTE WATER","authors":"Nilgün Poyraz, M. Mutlu","doi":"10.18036/AUBTDC.289555","DOIUrl":"https://doi.org/10.18036/AUBTDC.289555","url":null,"abstract":"Calisma kapsaminda iki farkli atik su aritma tesisinin cesitli unitelerinden ornek alinmis ve bu orneklerin pH ve element icerikleri belirlenmistir. Ozellikleri belirlenen orneklerden fenollu besiyerine inokulasyon yapilmis ve 19 izolat elde edilmistir. Bu izole edilen 19 izolattan morfolojik olarak farkli oldugu dusunulen 9 izolatin tanimlamasi yapilmistir. Izolatlar arasinda yogun olarak Pseudomonas turleri belirlenmistir. Tanimlanan bu 9 izolatin atik sulardaki aritim potansiyellerini degerlendirebilmek icin antibiyotik duyarlilik testi gerceklestirilmistir. Ayrica izolatlarin amilaz ve proteaz enzim uretim aktiviteleri degerlendirilmistir. Izolatlarin ofloksasin, azitromisin ve streptomisine duyarli olduklari belirlenmistir. Enzim uretim yetenekleri degerlendirildiginde ise yalnizca 2 izolat zayif proteaz aktivitesi gosterirken izolatlarin cogu amilaz aktivitesi gostermistir.","PeriodicalId":7738,"journal":{"name":"ANADOLU UNIVERSITY JOURNAL OF SCIENCE AND TECHNOLOGY –C Life Sciences and Biotechnology","volume":"6 1","pages":"64-74"},"PeriodicalIF":0.0,"publicationDate":"2017-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45196916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
EFFECTS OF CLIMATE CHANGE ON MOSQUITO-BORNE DISEASES 气候变化对蚊媒疾病的影响
ANADOLU UNIVERSITY JOURNAL OF SCIENCE AND TECHNOLOGY –C Life Sciences and Biotechnology Pub Date : 2017-08-31 DOI: 10.18036/aubtdc.296680
Yesim Polat, A. Yanıkoğlu, H. Cetin
{"title":"EFFECTS OF CLIMATE CHANGE ON MOSQUITO-BORNE DISEASES","authors":"Yesim Polat, A. Yanıkoğlu, H. Cetin","doi":"10.18036/aubtdc.296680","DOIUrl":"https://doi.org/10.18036/aubtdc.296680","url":null,"abstract":"Kuresel isinma, sera gazlarinin etkisinden dolayi ortalama yuzey sicakliginin artmasidir. Bu durum iklim modellerinin degismesine neden olmaktadir. Kuresel isinmanin bocek turlerinin dagilimi ve bollugu uzerindeki etkileri bilim insanlari tarafindan arastirilmaktadir. Bilimsel arastirma sonuclarina gore sivrisinekler ve sivrisinekler yoluyla bulasan hastaliklarin dagilimi ve sayisi da iklim degisikliginden etkilenmektedir. Bu calismada iklim degisikliginin Dang Hummasi, Sari Humma, Chikungunya, Zika Virusu, Bati Nil Virusu ve Sitma gibi sivrisinek kaynakli hastaliklar uzerindeki etkileri hakkinda senaryolar verilmektedir.","PeriodicalId":7738,"journal":{"name":"ANADOLU UNIVERSITY JOURNAL OF SCIENCE AND TECHNOLOGY –C Life Sciences and Biotechnology","volume":"6 1","pages":"86-94"},"PeriodicalIF":0.0,"publicationDate":"2017-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43041535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A REVIEW ON INTEIN MEDIATED RECOMBINANT PROTEIN PURIFICATION PROCESSES WITHOUT USE OF PROTEASE 不使用蛋白酶的重组蛋白纯化方法综述
ANADOLU UNIVERSITY JOURNAL OF SCIENCE AND TECHNOLOGY –C Life Sciences and Biotechnology Pub Date : 2017-08-31 DOI: 10.18036/AUBTDC.267017
Y. Ermurat
{"title":"A REVIEW ON INTEIN MEDIATED RECOMBINANT PROTEIN PURIFICATION PROCESSES WITHOUT USE OF PROTEASE","authors":"Y. Ermurat","doi":"10.18036/AUBTDC.267017","DOIUrl":"https://doi.org/10.18036/AUBTDC.267017","url":null,"abstract":"Bu calismada, yapay proteinlerin proteaz kullanilmadan saflastirilmasi sureclerinde yararlanilan inteinlerin ayrilma aktivitelerinin artirilmasi icin inteinler ve hedef proteinler uzerinde yapilan arastirmalar incelenmistir. Intein kullanimi ile protein ayrismasi uzerinde yapilan arastirmalar, intein ve protein saflastirma sureclerinin daha verimli hale getirilmesini amaclamaktadir. In vivo ve in vitro olarak gerceklesen sureclerde, intein ve hedef protein iceren poliklonal PCR urununun cogaltilmasi ve ekspres plazmidlerin klonlanan suslara tasinmasi islemleri yapilmaktadir. Inteinin proteinden ayrilma aktivitesi ve ayrilma orani sabiti kinetikleri, degisik pH, sicaklik ve suzme surelerinde belirlenmekte ve hedef proteinlere iliskili olarak karsilastirilmalari yapilmaktadir. Arastirmalarda degisik mikrobiyal dogal ve yapay inteinlerin protein ayristirma aktiviteleri incelenmis ve yapay Nostoc punctiforme DnaE C-inteinlerinin ayrisma aktivitelerinin en yuksek oldugu belirlenmistir. Calismalar amino asit eklenmesine daha yatkin olmasi nedeniyle C-inteinler uzerinde yogunlasmaktadir.","PeriodicalId":7738,"journal":{"name":"ANADOLU UNIVERSITY JOURNAL OF SCIENCE AND TECHNOLOGY –C Life Sciences and Biotechnology","volume":"6 1","pages":"95-102"},"PeriodicalIF":0.0,"publicationDate":"2017-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41377361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
ANTIOXIDATIVE RESPONSES IN Tubifex tubifex AGAINST THALLIUM INDUCED OXIDATIVE STRESS 管蝶对铊诱导氧化应激的抗氧化反应
ANADOLU UNIVERSITY JOURNAL OF SCIENCE AND TECHNOLOGY –C Life Sciences and Biotechnology Pub Date : 2017-08-31 DOI: 10.18036/AUBTDC.288885
G. KiliÇ, V. Kılıç
{"title":"ANTIOXIDATIVE RESPONSES IN Tubifex tubifex AGAINST THALLIUM INDUCED OXIDATIVE STRESS","authors":"G. KiliÇ, V. Kılıç","doi":"10.18036/AUBTDC.288885","DOIUrl":"https://doi.org/10.18036/AUBTDC.288885","url":null,"abstract":"Talyum (Tl)’un canli sistemler uzerindeki etkisi son yillarda onemli bir sorun haline gelmistir. Bununla birlikte, sucul sistemlerin Tl’a maruz kalmasinin sonuclari ile ilgili cok az bilgi mevcuttur. Bu calismanin amaci, sucul oligoket Tubifex tubifex ’in antioksidan biyolojik belirteclerinde ( metallotionein (MT), toplam glutatyon (GSH), glutatyon peroksidaz (GPx), glutatyon S-transferaz (GST) ve katalaz (CAT)), TI’un subletal dozlarina yanit olarak meydana gelen degisikliklerin arastirilmasidir. Deney gruplari 0.25, 0.5 ve 1 µg l -1’ lik konsantrasyonlarda, 7 ve 15 gunluk sureler boyunca talyum asetat’a maruz birakilmistir. MT seviyelerinde ilgi cekici bir azalma gozlenmistir. 0.25 µg l -1’ lik konsantrasyon toplam GSH, GPx ve GST aktiviteleri uzerinde etki gostermezken, 0.5 ve 1 µg l -1’ lik konsantrasyonlar toplam GSH ve GPx aktivitelerini 7 gunun sonunda induklemistir. Daha uzun maruz kalma suresi GPx ve GST aktiviteleri ile birlikte toplam GSH’un da tum konsantrasyonlarda azalmasina neden olmustur. CAT aktivitesinde tum deney gruplarinda gozlenen anlamli artisa ragmen, lipid peroksidasyonunun (LP) artan konsantrasyon ve sureye bagli olarak arttigi gozlenmistir. Sonuclar Tl ile induklenen oksidatif strese karsi, T. tubifex ’in antioksidan sisteminin olasi hareket mekanizmasini ortaya koymakta ve Tl’un sucul ekosistemlerdeki etkileri ile ilgili gelecekte yapilacak degerlendirmeler icin onemli bir bilgi kaynagi olusturmaktadir.","PeriodicalId":7738,"journal":{"name":"ANADOLU UNIVERSITY JOURNAL OF SCIENCE AND TECHNOLOGY –C Life Sciences and Biotechnology","volume":"6 1","pages":"75-85"},"PeriodicalIF":0.0,"publicationDate":"2017-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43040812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
DIVERSITY OF SAXICOLOUS, TERRICOLOUS, MUSCICOLOUS LICHENIZED AND LICHENICOLOUS FUNGI IN THE SÜNDİKEN MOUNTAINS sÜndİken山区土生、陆生、音乐性地衣真菌和地衣真菌的多样性
ANADOLU UNIVERSITY JOURNAL OF SCIENCE AND TECHNOLOGY –C Life Sciences and Biotechnology Pub Date : 2017-02-16 DOI: 10.18036/AUBTDC.291667
Y. Yavuz, A. Türk
{"title":"DIVERSITY OF SAXICOLOUS, TERRICOLOUS, MUSCICOLOUS LICHENIZED AND LICHENICOLOUS FUNGI IN THE SÜNDİKEN MOUNTAINS","authors":"Y. Yavuz, A. Türk","doi":"10.18036/AUBTDC.291667","DOIUrl":"https://doi.org/10.18036/AUBTDC.291667","url":null,"abstract":"Sundiken Daglari’nin saksikol, terrikol, muskikol liken ve likenikol mantar cesitliligine katkida bulunmak uzere yapilan bu calismada 229 liken ve likenikol mantar taksonu bulunmustur. Cercidospora crozalsiana, Immersaria usbekica, Rhizocarpon sublavatum, Scytinium biatorinum Turkiye’den ikinci kez kaydedilmistir. Bu calisma ile Eskisehir Ili’nden kaydedilen liken ve likenikol mantarlarin sayisi 361’e ulasmistir.","PeriodicalId":7738,"journal":{"name":"ANADOLU UNIVERSITY JOURNAL OF SCIENCE AND TECHNOLOGY –C Life Sciences and Biotechnology","volume":"6 1","pages":"1-13"},"PeriodicalIF":0.0,"publicationDate":"2017-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41379036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
THE APOPTOTIC EFFECTS OF TİTANİUM DİOXİDE ON A549 CELLS
ANADOLU UNIVERSITY JOURNAL OF SCIENCE AND TECHNOLOGY –C Life Sciences and Biotechnology Pub Date : 2017-02-16 DOI: 10.18036/AUBTDC.291668
A. Kaplan, G. Çiftçi, H. M. Kutlu
{"title":"THE APOPTOTIC EFFECTS OF TİTANİUM DİOXİDE ON A549 CELLS","authors":"A. Kaplan, G. Çiftçi, H. M. Kutlu","doi":"10.18036/AUBTDC.291668","DOIUrl":"https://doi.org/10.18036/AUBTDC.291668","url":null,"abstract":"Metal temelli bilesikler potansiyel etkilerinden ve az toksisiteye neden olduklarindan dolayi uzun zamandir kullanilmaktadir. Metal bilesik sisplatin cesitli kanser turlerine karsi tibbi olarak kullanilmis ve yan etkiler gostermistir. Biz bu calismada sisplatine alternatif ajan olarak dusundugumuz titanyum dioksitin A549 hucreleri uzerindeki etkilerini ve apoptotik mekanizmasini arastirdik. Titanyum dioksitin 24, 48 ve 72 saatlik surelerdeki zamana ve konsantrasyon araligina bagli olarak antiproliferatif etkilerini MTT canlilik analizini kullanarak belirledik. Titanyum dioksitin apoptozisi tetikledigini saptadik. Titanyum dioksitin IC 30 konsantrasyonu, 72 saat suresince A549 hucreleri uzerinde uygulandiginda erken ve gec apoptozisi tetikledigi belirlendi. Titanyum dioksitin IC 30 konsantrasyonu, 72 saat suresince A549 hucreleri uzerine uygulandiginda mitokondriyal membran potansiyelini azaltarak apoptozisi tetikledi. Buna ragmen kaspaz-3 aktivitesi gozlenmedi. Titanyum dioksit kaspazdan bagimsiz bir yol izleyerek apoptozisi tetiklemistir diyebiliriz. Titanyum dioksitin IC 30 konsantrasyonun 72 saatlik sure sonundaki hematoksilen ve eozin, TUNEL, BrdU, Bcl-2 ve Bax immunositokimyasal analizleri sonucunda  apoptotik indekslerinde artis belirlendi. Titanyum dioksitin IC 30 konsantrasyonun uygulandigi A549 hucrelerinde 72 saatlik sure sonunda konfokal ve TEM mikrokobisi kullanilarak cesitli apoptotik yapilar gozlendi. Bu sonuclar titanyum dioksitin A549 hucreleri uzerinde antiproliferatif ve apoptotik etkilerini ve sisplatine es potansiyel kemoterapotik bir ajan olabilecegini gostermektedir.","PeriodicalId":7738,"journal":{"name":"ANADOLU UNIVERSITY JOURNAL OF SCIENCE AND TECHNOLOGY –C Life Sciences and Biotechnology","volume":"6 1","pages":"38-54"},"PeriodicalIF":0.0,"publicationDate":"2017-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45890741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
DETECTION OF Cryptosporidium OOCYSTS BY LOOP MEDIATED ISOTHERMAL AMPLIFICATION (LAMP) IN SURFACE WATER FROM RIVER YESILIRMAK AND STREAM TERSAKAN (SAMSUN-AMASYA) 环介导等温扩增(LAMP)法检测YESILIRMAK河和TERSAKAN河地表水隐孢子虫卵囊
ANADOLU UNIVERSITY JOURNAL OF SCIENCE AND TECHNOLOGY –C Life Sciences and Biotechnology Pub Date : 2017-02-16 DOI: 10.18036/aubtdc.269434
Z. Kolören, Burak K. Delioğlu, B. Taş
{"title":"DETECTION OF Cryptosporidium OOCYSTS BY LOOP MEDIATED ISOTHERMAL AMPLIFICATION (LAMP) IN SURFACE WATER FROM RIVER YESILIRMAK AND STREAM TERSAKAN (SAMSUN-AMASYA)","authors":"Z. Kolören, Burak K. Delioğlu, B. Taş","doi":"10.18036/aubtdc.269434","DOIUrl":"https://doi.org/10.18036/aubtdc.269434","url":null,"abstract":"Amasya il merkezi ve ilceleri Suluova, Tasova’dan, Samsun ilceleri Havza ve Carsamba’dan gecen Tersakan Cayi ve Yesilirmak Nehri’nden alinan su orneklerinde Cryptosporidium turleri Loop Mediated Isothermal Amplification (LAMP) yontemi kullanilarak arastirilmistir. 2010 Aralik-2011 Kasim doneminde 14 istasyondan her ay duzenli su ornekleri alinarak bu orneklere LAMP  testi uygulanmistir. On dort istasyonun 10’unda Cryptosporidium ookistlerine LAMP yontemiyle rastlanilmistir. Cryptosporidium ookistleriyle kontamineli istasyonlar: Tersakan–Havza; Tersakan-Suluova, Celtek; Tersakan-Kanlidere; Tersakan-Bogazkoy; Tersakan-Karasu; Tersakan-son; Yesilirmak-Amasya Koprusu; Yesilirmak-Yassical; Yesilirmak-Durucasu; Yesilirmak-Tasova’dir. Arastirilan tum istasyonlarin icinde Tersakan–Suluova, Celtek ve Tersakan-Bogazkoy istasyonlari Cryptosporidium ookistleri acisindan en kirli istasyonlar olarak belirlenmistir. Bununla beraber Amasya ve Samsun’dan gecen Yesilirmak Nehri’nin Tersakan Cayi’na oranla daha temiz oldugu saptanmistir . Tersakan Cayi’ndaki Cryptosporidium kontaminasyonun fazlaligi, hayvan besiciliginin Havza and Suluova ilcelerinde yerlesim yerlerine cok yakin mesafelerde yapilmasina; evsel ve tarimsal atik sularin hicbir isleme tabi tutulmadan direk olarak Tersakan Cayi’na desarj edilmesine baglanmaktadir.","PeriodicalId":7738,"journal":{"name":"ANADOLU UNIVERSITY JOURNAL OF SCIENCE AND TECHNOLOGY –C Life Sciences and Biotechnology","volume":"6 1","pages":"31-37"},"PeriodicalIF":0.0,"publicationDate":"2017-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41698773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
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