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A monophyletic origin of heart-predominant lactate dehydrogenase (LDH) isozymes of gnathostome vertebrates: evidence from the cDNA sequence of the spiny dogfish (Squalus acanthias) LDH-B. 啮齿脊椎动物心脏优势乳酸脱氢酶(LDH)同工酶的单系起源:来自棘角鲨(Squalus acanthias) LDH- b cDNA序列的证据
Molecular marine biology and biotechnology Pub Date : 1998-06-01
D W Stock, D A Powers
{"title":"A monophyletic origin of heart-predominant lactate dehydrogenase (LDH) isozymes of gnathostome vertebrates: evidence from the cDNA sequence of the spiny dogfish (Squalus acanthias) LDH-B.","authors":"D W Stock,&nbsp;D A Powers","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Duplication of a single lactate dehydrogenase locus early in vertebrate evolution has been proposed to have given rise to Ldh-A and Ldh-B, the encoded isozymes of which predominate in skeletal and heart muscle, respectively. This view has been challenged recently by phylogenetic analyses of LDH sequences. One question that has been raised is whether the heart-predominant isozyme (LDH-B) of cartilaginous fishes is orthologous to that of bony fishes and their derivatives. To address this issue, we determined the complementary DNA sequence of the LDH-B of the chondrichthyan Squalus acanthias. Phylogenetic analysis of this and other LDH isozyme sequences provided strong support for a single origin of LDH-Bs prior to the divergence of cartilaginous and bony fishes.</p>","PeriodicalId":77273,"journal":{"name":"Molecular marine biology and biotechnology","volume":"7 2","pages":"160-4"},"PeriodicalIF":0.0,"publicationDate":"1998-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20547058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Molecular cloning of a novel interferon regulatory factor in Japanese flounder, Paralichthys olivaceus. 一种新型干扰素调控因子在牙鲆中的分子克隆。
Molecular marine biology and biotechnology Pub Date : 1998-06-01
T Yabu, H Hirose, I Hirono, T Katagiri, T Aoki, E Yamamoto
{"title":"Molecular cloning of a novel interferon regulatory factor in Japanese flounder, Paralichthys olivaceus.","authors":"T Yabu,&nbsp;H Hirose,&nbsp;I Hirono,&nbsp;T Katagiri,&nbsp;T Aoki,&nbsp;E Yamamoto","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A complementary DNA library was constructed in lambda ZAP II using messenger RNA from the leukocytes of some heterocloned Japanese flounder, Paralichthys olivaceus, that had been artificially infected with Hirame rhabdovirus (HRV). A cloned flounder interferon regulatory factor (designated fIRF) cDNA was found to be 1746 bp in length, with an open reading frame of 297 amino acids. The overall amino acid sequence of fIRF had approximately 40% identity with the previously reported avian and mammalian IRF-1s and IRF-2s. The fIRF sequence was most similar to that recorded for the chicken IRF-1. Amino acid sequence identities between the DNA-binding domain of the fIRF and that of both chicken IRF-1 and chicken IRF-2 were 72.3%. The DNA-binding domain of fIRF contained the repeated tryptophan motif that is characteristic of members of the IRF family. The mRNA of fIRF was detected in various tissues by reverse transcription-polymerase chain reaction (RT-PCR). The fIRF was transcribed mainly in the intestine, ovary, muscle, liver, heart and spleen, while it was minimally transcribed in the brain and kidney. When Japanese flounder were injected with HRV, the relative expression of fIRF mRNA was found to increase and peak 3 days after injection. The quantities of the fIRF mRNA increased to levels that were 7.5-fold higher than those of noninjected fish. In addition, when Japanese flounder were injected with Edwardsiella tarda, the expression of fIRF mRNA showed increases 2, 3, and 4 days after injection. The quantities of the fIRF mRNA on those days represented approximately 6-, 15-, and 14-fold increases, respectively, over the levels in noninjected fish.</p>","PeriodicalId":77273,"journal":{"name":"Molecular marine biology and biotechnology","volume":"7 2","pages":"138-44"},"PeriodicalIF":0.0,"publicationDate":"1998-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20547057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Yeasts isolated from the intestine of rainbow trout adhere to and grow in intestinal mucus. 从虹鳟鱼肠道中分离出的酵母菌附着在肠道粘液中生长。
Molecular marine biology and biotechnology Pub Date : 1998-06-01
T Andlid, R Vázquez-Juárez, L Gustafsson
{"title":"Yeasts isolated from the intestine of rainbow trout adhere to and grow in intestinal mucus.","authors":"T Andlid,&nbsp;R Vázquez-Juárez,&nbsp;L Gustafsson","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The yeast strains Saccharomyces cerevisiae CBS 7764 and Debaryomyces hansenii Hf1 (CBS 8339), isolated from the intestine of rainbow trout, were studied with respect to adhesion to and growth in fish intestinal mucus. The level of adhesion was dependent on the physiologic state of the yeast culture. Growing cells of both strains adhered more strongly than nongrowing cells. This correlates with a previously shown shift in cell surface hydrophobicity of these yeasts. In addition, forces other than hydrophobic interactions may participate, as all strains tested adhered more strongly to the membrane lipid phosphatidylserine than to phosphatidylcholine and phosphatidylethanolamine. Debaryomyces hansenii Hf1 also adhered to the most hydrophobic of the neutral lipids present in mucus, while no adhesion was observed to the other neutral lipids or to the hydrophilic silica gel, again suggesting hydrophobic interactions. Finally, the fish-isolated yeasts grew rapidly in isolated fish intestinal mucus as the sole source of energy and nutrients.</p>","PeriodicalId":77273,"journal":{"name":"Molecular marine biology and biotechnology","volume":"7 2","pages":"115-26"},"PeriodicalIF":0.0,"publicationDate":"1998-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20547055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Purification and characterization of pancreatic elastase from North Atlantic salmon (Salmo salar). 北大西洋大马哈鱼胰腺弹性酶的纯化及特性研究。
Molecular marine biology and biotechnology Pub Date : 1998-06-01
G I Berglund, A O Smalås, H Outzen, N P Willassen
{"title":"Purification and characterization of pancreatic elastase from North Atlantic salmon (Salmo salar).","authors":"G I Berglund,&nbsp;A O Smalås,&nbsp;H Outzen,&nbsp;N P Willassen","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>An elastase I-like enzyme was purified to homogeneity from the pyloric caeca of North Atlantic salmon (Salmo salar) and compared with porcine elastase I. The molecular weight and isoelectric point were estimated to be 27 kDa and over 9.3, respectively. The pH optimum was between 8.0 and 9.5, and the enzyme was unstable at pH values below 4. Kinetic properties examined using Suc-(Ala)3-p-nitroanilide showed that the catalytic efficiency of salmon elastase was about 2.5 times higher than that of porcine elastase. Furthermore, the salmon enzyme was less stable at lower pH values and temperatures than the porcine enzyme. The preference for amino acids at the primary binding site was found to be different from that of the porcine elastase. The salmon elastase binding pocket seems to prefer more branched aliphatic residues than the porcine elastase.</p>","PeriodicalId":77273,"journal":{"name":"Molecular marine biology and biotechnology","volume":"7 2","pages":"105-14"},"PeriodicalIF":0.0,"publicationDate":"1998-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20547054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Molecular systematics of shrimp (Decapoda: Bresiliidae) from deep-sea hydrothermal vents, I: Enigmatic "small orange" shrimp from the Mid-Atlantic Ridge are juvenile Rimicaris exoculata. 深海热液口对虾(十足目:贝蛤科)的分子系统学，1:大西洋中脊神秘的“小橙色”对虾是幼年外孔虾。
Molecular marine biology and biotechnology Pub Date : 1998-06-01
T M Shank, R A Lutz, R C Vrijenhoek
{"title":"Molecular systematics of shrimp (Decapoda: Bresiliidae) from deep-sea hydrothermal vents, I: Enigmatic \"small orange\" shrimp from the Mid-Atlantic Ridge are juvenile Rimicaris exoculata.","authors":"T M Shank,&nbsp;R A Lutz,&nbsp;R C Vrijenhoek","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Independent species descriptions of a \"small orange\" caridean shrimp found at deep-sea hydrothermal vents along the Mid-Atlantic Ridge have created the synonymous names Iorania concordia Vereshchaka 1996b and Rimicaris aurantiaca Martin et al. 1997. Our genetic analyses involving allozymes and mitochondrial DNA sequences reveal that the \"small orange\" shrimp described in these studies are a juvenile form of Rimicaris exoculata Williams and Rona, a species commonly found at these sites. In light of this result, we reconsider the life history and ecologic characteristics of juvenile and adult stages of Rimicaris exoculata.</p>","PeriodicalId":77273,"journal":{"name":"Molecular marine biology and biotechnology","volume":"7 2","pages":"88-96"},"PeriodicalIF":0.0,"publicationDate":"1998-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20547153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A DNA probe for identification of larvae of the commercial surfclam (Spisula solidissima). 商业蛤(Spisula solidissima)幼虫的DNA探针鉴定。
Molecular marine biology and biotechnology Pub Date : 1998-06-01
J L Bell, J P Grassle
{"title":"A DNA probe for identification of larvae of the commercial surfclam (Spisula solidissima).","authors":"J L Bell,&nbsp;J P Grassle","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A molecular DNA probe was developed for identification of larvae of the commercial surfclam Spisula solidissima (family Mactridae), to distinguish early-stage veligers from larvae of other common bivalve species in a study of surfclam settlement and recruitment on the New Jersey continental shelf. For discrimination of S. solidissima from other common bivalve species at the study site (almost all of which belong to different families), an oligonucleotide designed from the nucleotide sequence of the 18S ribosomal RNA gene provides a useful and sensitive family-specific probe and primer. For discrimination between S. solidissima and Mulinia lateralis (both members of Mactridae), the 18S rRNA gene was examined by restriction fragment length polymorphism (RFLP) analysis. A combination of the DNA probe and RFLP analysis provides a positive identification of S. solidissima and M. lateralis larvae in coastal plankton samples.</p>","PeriodicalId":77273,"journal":{"name":"Molecular marine biology and biotechnology","volume":"7 2","pages":"127-37"},"PeriodicalIF":0.0,"publicationDate":"1998-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20547056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
ROn-1 SINEs: a tRNA-derived, short interspersed repetitive DNA element from Oreochromis niloticus and its species-specific distribution in Old World cichlid fishes. ROn-1 SINEs:一种由trna衍生的短穿插重复DNA元件，来自于nilochromis及其在东半球慈鲷鱼中的特定分布。
Molecular marine biology and biotechnology Pub Date : 1998-03-01
L J Bryden, E M Denovan-Wright, J M Wright
{"title":"ROn-1 SINEs: a tRNA-derived, short interspersed repetitive DNA element from Oreochromis niloticus and its species-specific distribution in Old World cichlid fishes.","authors":"L J Bryden,&nbsp;E M Denovan-Wright,&nbsp;J M Wright","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A SINE-like repetitive element (ROn-1) has been cloned from the tilapiine cichlid fish Oreochromis niloticus. The element is 345 base pairs (bp) in length and consists of a transfer-RNA-like domain with putative RNA polymerase III recognition sequences, a tRNA-unrelated region, and a poly(A) tail. Approximately 6000 copies of ROn-1 occur in the haploid genome of O. niloticus. Southern blot analysis revealed that ROn-1 is an abundant element in the genomes of many African cichlid fishes, but absent from the genome of the Indian cichlid Etroplus.</p>","PeriodicalId":77273,"journal":{"name":"Molecular marine biology and biotechnology","volume":"7 1","pages":"48-54"},"PeriodicalIF":0.0,"publicationDate":"1998-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20517916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Direct sequencing of genomic DNA for characterization of a satellite DNA in five species of eastern Pacific abalone. 东太平洋五种鲍鱼卫星DNA的直接测序。
Molecular marine biology and biotechnology Pub Date : 1998-03-01
M E Muchmore, G W Moy, W J Swanson, V D Vacquier
{"title":"Direct sequencing of genomic DNA for characterization of a satellite DNA in five species of eastern Pacific abalone.","authors":"M E Muchmore,&nbsp;G W Moy,&nbsp;W J Swanson,&nbsp;V D Vacquier","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A tandemly repeated satellite DNA of 290-291 base pairs (bp) was identified by SalI digestion of genomic DNA of five species of Eastern Pacific (California) abalone (genus, Haliotis). Following cloning and sequencing of one repeat unit from one species, the consensus sequences of this satellite were determined for five species by directly sequencing genomic DNA using satellite-specific primers. Phylogenetic trees of the consensus satellite sequences had the same topology as trees constructed for two abalone sperm acrosomal proteins. In 12 randomly picked clones of the Red abalone (H. rufescens) SalI satellite, 16 positions varied, the variation being spread throughout the sequence. GenBank database searches found no significant similarities between this satellite and known sequences. Southern analysis showed that all 290-bp SalI repeats were excised from genomic DNA by Sau3A1 digestion. The tandem arrangement of satellite repeats was confirmed by sequencing through the SalI site into the next repeat using genomic DNA as template, time-dependent appearance of DNA ladders with an approximate 300-bp spacing in SalI digests of genomic DNA, and ladders of bands with an approximate 300-bp spacing generated by polymerase chain reaction (PCR) using genomic DNA as template. In the Red abalone, the 290-bp SalI satellite represents approximately 0.5% of total DNA, equivalent to approximately 28,000 copies per haploid genome. The species-specific consensus sequence of this satellite, obtained directly using genomic DNA as the sequencing template, provides a molecular marker that could be used for identification of hybrid parentage, taxonomy, population identification, and forensic studies.</p>","PeriodicalId":77273,"journal":{"name":"Molecular marine biology and biotechnology","volume":"7 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"1998-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20518032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Restriction digest of PCR-amplified mtDNA from fin clips is an assay for sequence genetic "tags" among hundreds of fish in wild populations. 从鱼鳍夹中提取pcr扩增mtDNA的限制性酶切文摘是对野生种群中数百种鱼类的序列遗传“标签”进行分析的方法。
Molecular marine biology and biotechnology Pub Date : 1998-03-01
R R Wilson, K A Donaldson
{"title":"Restriction digest of PCR-amplified mtDNA from fin clips is an assay for sequence genetic \"tags\" among hundreds of fish in wild populations.","authors":"R R Wilson,&nbsp;K A Donaldson","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>We tested the feasibility of recovering from wild populations mitochondrial DNA genetic \"tags\" that might result from release of hatchery-reared fingerlings for marine stock enhancement. A practical, nondestructive method of genetic \"tag\" recovery would allow quantitative assessment of the efficacy of such programs. A rare mtDNA variant with two sites each instead of one for TaqI and RsaI in the control region was used as a mock genetic \"tag\" in snook (Centropomus undecimalis). We assayed for the variant among nearly 900 fish by restriction digests of DNA amplified by polymerase chain reaction (PCR) obtained from fin-clip extracts, finding only a single second example of the variant. Thus, mtDNA \"tags\" can be nondestructively assayed for with specificity among hundreds of individuals.</p>","PeriodicalId":77273,"journal":{"name":"Molecular marine biology and biotechnology","volume":"7 1","pages":"39-47"},"PeriodicalIF":0.0,"publicationDate":"1998-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20517915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Structure and expression of activin genes in rainbow trout. 虹鳟激活素基因的结构与表达。
Molecular marine biology and biotechnology Pub Date : 1998-03-01
T Tada, I Hirono, T Aoki, F Takashima
{"title":"Structure and expression of activin genes in rainbow trout.","authors":"T Tada,&nbsp;I Hirono,&nbsp;T Aoki,&nbsp;F Takashima","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Activins are dimeric members of the transforming growth factor-beta (TGF-beta) superfamily. By using the polymerase chain reaction (PCR), we have cloned and sequenced activin beta A and beta B genes encoding the mature region of the peptides from the rainbow trout genomic DNA. Two forms of beta A and a single form of beta B-subunits were found. There is high identity with mammalian counterparts; the two rainbow trout beta A-subunits have more than 75% nucleotide sequence identity with the human beta A-subunit, and the beta B-subunit had 82% sequence identity with the human beta B-subunit. Expression of rainbow trout activin genes was examined by reverse transcription-PCR (RT-PCR). The major expression tissue of rainbow trout activin was ovary and brain at the messenger RNA level, and the major expression subunit of rainbow trout activin was the beta B subunit.</p>","PeriodicalId":77273,"journal":{"name":"Molecular marine biology and biotechnology","volume":"7 1","pages":"72-7"},"PeriodicalIF":0.0,"publicationDate":"1998-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20517918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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