Molecular marine biology and biotechnology最新文献

Molecular cloning and evolution of transferrin cDNAs in salmonids. 鲑鱼转铁蛋白cdna的克隆与进化。

Molecular marine biology and biotechnology Pub Date : 1998-12-01

J Y Lee, T Tada, I Hirono, T Aoki

{"title":"Molecular cloning and evolution of transferrin cDNAs in salmonids.","authors":"J Y Lee, T Tada, I Hirono, T Aoki","doi":"","DOIUrl":"","url":null,"abstract":"Transferrin complementary DNAs were cloned from the livers of seven species in three genera of salmonids (kokanee salmon Oncorhynchus nerka, amago salmon Oncorhynchus masou ishikawa, masu salmon Oncorhynchus masou masou, Japanese char Salvelinus pluvius, brook trout Salvelinus fontinalis, lake trout Salvelinus namaycush, and brown trout Salmo trutta) subsequent to polymerase chain reaction amplification with primers derived from conserved regions of transferrin cDNA sequences. The transferrin cDNAs of the seven species of salmonids had sizes of 2.2 to 2.4 kb and encoded an open reading frame consisting of 691 amino acids with a putative signal peptide of 18 amino acids. The alignment of salmonid transferrin cDNAs showed a duplicated structure and conserved anion-binding residues, iron-binding residues, and cysteine residues for disulfide bridges. The deduced amino acid sequences of the seven salmonid transferrin cDNAs share 85% to 99% homology. A phylogenetic tree of amino acid sequences of transferrin cDNAs from salmonids showed that the relationship among the three genera of salmonids (Oncorhynchus, Salvelinus, and Salmo) is well correlated with that derived from classic morphologic and genetic analyses.","PeriodicalId":77273,"journal":{"name":"Molecular marine biology and biotechnology","volume":"7 4","pages":"287-93"},"PeriodicalIF":0.0,"publicationDate":"1998-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20799534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficient gene transfer into Xiphophorus muscle and melanoma by injection of supercoiled plasmid DNA. 通过注射超螺旋质粒DNA高效地将基因转移到剑骨肌和黑色素瘤。

Molecular marine biology and biotechnology Pub Date : 1998-12-01

P M Schulte, D A Powers, M Schartl

{"title":"Efficient gene transfer into Xiphophorus muscle and melanoma by injection of supercoiled plasmid DNA.","authors":"P M Schulte, D A Powers, M Schartl","doi":"","DOIUrl":"","url":null,"abstract":"Muscle and melanoma tissue of fish in the genus Xiphophorus were examined for their ability to take up and express foreign DNA. Supercoiled plasmid DNA containing a firefly luciferase reporter gene with expression driven by the cytomegalovirus enhancer and thymidylate kinase promoter was directly injected into the muscle or melanoma of individual Xiphophorus. Expression levels gradually increased to a maximum at 6 days after injection in both tissues, and this level was maintained for at least 10 days after injection. In both muscle and melanoma, there was a clear relationship between dose injected and reporter gene activity, with maximal expression at a dose of 20 microg of plasmid injected. At higher doses expression levels declined, suggesting the possibility that the uptake mechanism can be inhibited by high concentrations of DNA. Histochemical localization using a beta-galactosidase construct revealed high expression of the enzyme in isolated muscle fibers. The activity of a second coinjected reporter gene, sea pansy (Renilla reniformis) luciferase, was highly correlated with the activity of the firefly luciferase reporter gene in both tissues (R2 >.940), suggesting that the majority of variation between samples results from variation in overall DNA uptake between individuals. When firefly luciferase activity is expressed as a function of activity of the coinjected reporter, the variation between samples is greatly reduced. As a result, small differences in activity between constructs can be detected. This demonstrates the usefulness of the system for gene expression analysis in vivo.","PeriodicalId":77273,"journal":{"name":"Molecular marine biology and biotechnology","volume":"7 4","pages":"241-7"},"PeriodicalIF":0.0,"publicationDate":"1998-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20799574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation and characterization of lectin from Thai marine crab (Scylla serrata) with binding specificity to sialoglycoconjugates and its application. 泰国海蟹(Scylla serrata)唾液糖结合特异性凝集素的分离、鉴定及其应用。

Molecular marine biology and biotechnology Pub Date : 1998-12-01

P Kongtawelert

{"title":"Isolation and characterization of lectin from Thai marine crab (Scylla serrata) with binding specificity to sialoglycoconjugates and its application.","authors":"P Kongtawelert","doi":"","DOIUrl":"","url":null,"abstract":"A lectin from Thai marine carb (Scylla serrata) hemolymph has been isolated and purified by affinity column chromatography and preparative electrophoresis. The amino acid composition and 10 amino-terminal residues have been deduced, and its reactivities have been studied using a biotin labeling technique. A method for the determination of sialoglycoconjugates in human serum is described using this lectin. The principle is based on the reaction between the sialoglycoconjugates and biotinylated lectin. The bovine submaxillary mucin (BSM) is immobilized on polystyrene microplate. The unknown sample or sialoglycoconjugate (BSM equivalent) standards, together with excess biotinylated purified lectin (B-lectin), are then added. The B-lectin that binds to the immobilized BSM is then incubated with the peroxidase-conjugated monoclonal antibiotin antibody, and the color that develops after the addition of enzyme substrate is determined by light absorption using a microplate reader. The assay is not only convenient and reliable, but also capable of measuring sialoglycoconjugates in solution at the submicrogram level. It was used in determining the sialoglycoconjugates in human serum from normal subjects and samples positive for carcinoembryonic antigen.","PeriodicalId":77273,"journal":{"name":"Molecular marine biology and biotechnology","volume":"7 4","pages":"280-6"},"PeriodicalIF":0.0,"publicationDate":"1998-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20799533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microsatellite analysis of the population structure of a vancouver island sockeye salmon (Oncorhynchus nerka) stock complex using nondenaturing gel electrophoresis 非变性凝胶电泳技术对温哥华岛红鲑种群结构的微卫星分析

Molecular marine biology and biotechnology Pub Date : 1998-12-01

Nelson, Beacham, Small

引用次数: 0

Evidence of multiple transcription initiation and termination sites within the rDNA intergenic spacer and rRNA readthrough transcription in the urochordate Herdmania curvata. 在尾脊索动物弯曲Herdmania基因间间隔和rRNA读通转录中存在多个转录起始和终止位点的证据。

Molecular marine biology and biotechnology Pub Date : 1998-12-01

B M Degnan, J Yan, M F Lavin

{"title":"Evidence of multiple transcription initiation and termination sites within the rDNA intergenic spacer and rRNA readthrough transcription in the urochordate Herdmania curvata.","authors":"B M Degnan, J Yan, M F Lavin","doi":"","DOIUrl":"","url":null,"abstract":"Analysis of the structure of the urochordate Herdmania curvata ribosomal DNA intergenic spacer (IGS) and its role in transcription initiation and termination suggests that rRNA gene regulation in this chordate differs from that in vertebrates. A cloned H. curvata IGS is 1881 bp and composed predominantly of two classes of similar repeat sequences that largely alternate in a tandem array. Southern blot hybridization demonstrates that the IGS length variation within an individual and population is largely the result of changes in internal repeat number. Nuclease S1 mapping and primer extension analyses suggest that there are two transcription initiation sites at the 3' end of the most 3' repetitive element; these sites are 6 nucleotides apart. Unlike mouse, Xenopus, and Drosophila, there is no evidence of transcription starting elsewhere in the IGS. Most sequence differences between the promoter repeat and the other internal repeats are in the vicinity of the putative initiation sites. As in Drosophila, nuclease S1 mapping of transcription termination sites suggest that there is not a definitive stop site and a majority of the pre-rRNAs read through a substantial portion of the IGS. Some transcription appears to proceed completely through the promoter repeat into the adjacent rDNA unit. Analysis of oocyte RNA by reverse transcription-polymerase chain reaction (RT-PCR) confirms that readthrough transcription into the adjacent rDNA unit is occurring in some small IGS length variants; there is no evidence of complete readthrough of IGSs larger than 1.0 kb.","PeriodicalId":77273,"journal":{"name":"Molecular marine biology and biotechnology","volume":"7 4","pages":"294-302"},"PeriodicalIF":0.0,"publicationDate":"1998-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20799535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Production of androgenetic haploids in zebrafish with ultraviolet light. 紫外线照射下斑马鱼雄激素单倍体的产生。

Molecular marine biology and biotechnology Pub Date : 1998-12-01

A R Ungar, K A Helde, R T Moon

引用次数: 0

Sensitive detection of Renibacterium salmoninarum in whole fry, blood, and other tissues of pacific salmon by reverse transcription-polymerase chain reaction. 逆转录-聚合酶链反应灵敏检测太平洋鲑鱼全苗、血液及其他组织中的沙门氏菌。

Molecular marine biology and biotechnology Pub Date : 1998-12-01

L D Rhodes, W B Nilsson, M S Strom

引用次数: 0

The translational start sites of jawless and cartilaginous fish genes. 无颌鱼和软骨鱼基因的翻译起始位点。

Molecular marine biology and biotechnology Pub Date : 1998-12-01

G Smutzer, L L Chamberlin

引用次数: 0

Genetic variation of the european eel (Anguilla anguilla) 欧洲鳗(Anguilla Anguilla)的遗传变异

Molecular marine biology and biotechnology Pub Date : 1998-12-01

Lintas, Hirano, Archer

引用次数: 0

Effect of exogenous DNA microinjection on early development response of the seabream (Sparus aurata). 外源DNA微注射对海鲷(Sparus aurata)早期发育反应的影响

Molecular marine biology and biotechnology Pub Date : 1998-12-01

S García-Pozo, J Béjar, M Shaw, M C Alvarez

{"title":"Effect of exogenous DNA microinjection on early development response of the seabream (Sparus aurata).","authors":"S García-Pozo, J Béjar, M Shaw, M C Alvarez","doi":"","DOIUrl":"","url":null,"abstract":"DNA transfer techniques allow genetic manipulation of commercial fish. However, marine species have received little attention because of their difficult zootechnical requirements. The seabream (Sparus aurata) has become one of the most important species in the aquaculture of Mediterranean countries, and the development of suitable DNA transfer procedures represents a main step in its genetic improvement. To assess the response of the seabream to exogenous DNA, naturally fertilized eggs were injected with the plasmids pCMV-CAT, pCMVTklacZ, and pEGFP-N1, in supercoiled and linearized forms. Embryo and larval survival, DNA fate, and reporter gene expression were analyzed during early development. The survival results indicate that microinjection is an effective transfer method in spite of the unfavorable conditions. Linearized plasmids were more efficiently polymerized than supercoiled ones; however, no significant differences were detected either in their persistence or in their expression levels. Reporter gene expression was initiated after mid-blastula transition. The duration of transient expression varied between the promoter-gene combinations, and no integration of transgenes into fish chromosomes was detected. Results suggest that the main factor affecting the persistence and expression of DNA seems to be related to developmental processes. Among the markers used, CAT proved to be the most sensitive, but GFP had obvious methodologic advantages over the spatial marker lacZ. The usefulness of GFP for diagnosis of transgenesis is enhanced by the transparency of embryos and larvae in S. aurata.","PeriodicalId":77273,"journal":{"name":"Molecular marine biology and biotechnology","volume":"7 4","pages":"248-58"},"PeriodicalIF":0.0,"publicationDate":"1998-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20799575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0