Molecular marine biology and biotechnology最新文献_第2页

Isolation of a cDNA encoding a homologue of ribosomal protein L26 in the decapod crustacean Penaeus japonicus. 日本对虾核糖体蛋白L26同源基因cDNA的分离。

Molecular marine biology and biotechnology Pub Date : 1998-12-01

T Watanabe

引用次数: 0

Characterization of a polymorphic protein localized to vascular epithelium in Botryllus schlosseri: role in tunic synthesis? 一种定位于血管上皮的多态蛋白的表征:在束膜合成中的作用?

Molecular marine biology and biotechnology Pub Date : 1998-09-01

M B Fagan, I L Weissman

引用次数: 0

Cloning of major histocompatibility complex (Mhc) genes from threespine stickleback, Gasterosteus aculeatus. 刺棘鱼主要组织相容性复合体基因的克隆。

Molecular marine biology and biotechnology Pub Date : 1998-09-01

A Sato, F Figueroa, C O'hUigin, N Steck, J Klein

{"title":"Cloning of major histocompatibility complex (Mhc) genes from threespine stickleback, Gasterosteus aculeatus.","authors":"A Sato, F Figueroa, C O'hUigin, N Steck, J Klein","doi":"","DOIUrl":"","url":null,"abstract":"The threespine stickleback Gasterosteus aculeatus is an important model in evolutionary and ethologic studies. Its utility would greatly be increased by the availability of molecular markers distinguishing individuals and populations. Such markers can be provided by the major histocompatibility complex (Mhc) genes, which are well known for their extensive polymorphism. In the present study, both class I and class II B Mhc genes have been identified and sequenced. Fifteen distinct class I exon 2 and exon 3 sequences were obtained and assigned to 12 loci on the basis of intron 2 length differences. Some of the loci appear to be related to class I loci identified previously in cichlid fish. The intron 2 sequences and insertions/deletions in exon 2 group the loci into three families (with one family divided further into two subfamilies) derived from different ancestral genes. The ancestors presumably diverged from one another before the divergence of Gasterosteiformes from Perciformes. The 12 distinct class II B sequences may be derived from six loci, which are, however, closely related to one another in both exonic and intronic parts and may have diverged from a single common ancestor after the divergence of Gasterosteiformes from Perciformes. The intron 2 of some of the class I genes contains two microsatellites that can be used as markers, in addition to the polymorphism of the Mhc genes in their exonic regions.","PeriodicalId":77273,"journal":{"name":"Molecular marine biology and biotechnology","volume":"7 3","pages":"221-31"},"PeriodicalIF":0.0,"publicationDate":"1998-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20617848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of a teleost insulin-like growth factor II (IGF-II) gene: evidence for promoter CCAAT/enhancer-binding protein (C/EBP) sites, and the presence of hepatic C/EBP. 硬骨鱼胰岛素样生长因子II (IGF-II)基因的表征:启动子CCAAT/增强子结合蛋白(C/EBP)位点的证据，以及肝C/EBP的存在

Molecular marine biology and biotechnology Pub Date : 1998-09-01

M J Shamblott, S Leung, M W Greene, T T Chen

{"title":"Characterization of a teleost insulin-like growth factor II (IGF-II) gene: evidence for promoter CCAAT/enhancer-binding protein (C/EBP) sites, and the presence of hepatic C/EBP.","authors":"M J Shamblott, S Leung, M W Greene, T T Chen","doi":"","DOIUrl":"","url":null,"abstract":"Insulin-like growth factor I (IGF-I) and IGF-II, acting under the regulatory control of growth hormone, are the principal mediators of vertebrate growth. We have previously demonstrated that like humans, but unlike rodents, rainbow trout maintain high hepatic IGF-II messenger RNA levels into adulthood. Here we describe a rainbow trout IGF-II gene with a proximal promoter that contains two CCAAT/enhancer-binding protein (C/EBP) binding sites (TCBS1 and TCBS2). Nuclear proteins corresponding in size to rat C/EBPalpha and C/EBPbeta were detected on Western immunoblots of growth-hormone-treated and mock-treated trout liver extracts. Electrophoretic mobility shift assay of these nuclear extracts further suggests the presence of C/EBPs in trout liver and confirms the ability of TCBS1 to form a complex with trout liver nuclear proteins that is identical in mobility and specificity to that formed by a mammalian consensus CBS construct. In both Western blot and mobility assay results, the growth-hormone-treated trout livers appeared to have a greater accumulation of C/EBP, suggesting a molecular mechanism by which growth hormone can influence the level of serum IGF-II.","PeriodicalId":77273,"journal":{"name":"Molecular marine biology and biotechnology","volume":"7 3","pages":"181-90"},"PeriodicalIF":0.0,"publicationDate":"1998-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20618612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of an A/T-rich family of sequences from channel catfish (Ictalurus punctatus). 海峡鲶鱼(Ictalurus punctatus) A/ t富家族序列的鉴定。

Molecular marine biology and biotechnology Pub Date : 1998-09-01

Z Liu, P Li, R A Dunham

{"title":"Characterization of an A/T-rich family of sequences from channel catfish (Ictalurus punctatus).","authors":"Z Liu, P Li, R A Dunham","doi":"","DOIUrl":"","url":null,"abstract":"A family of highly repetitive DNA sequences referred to as Xba elements was identified and characterized from the channel catfish (Ictalurus punctatus) genome. The Xba elements represent about 5% to 6% of the total genomic DNA of the channel catfish. The Xba elements are distributed specifically in the channel catfish and blue catfish (I. furcatus), but not in closely related species such as white catfish (Ameiurus catus) and flathead catfish (Pylodictus olivaris). These Xba elements are arranged as head-to-tail tandem repeats. Seven sequences were sequenced. They are A/T-rich (over 65%). Each sequence contains four copies of the ATTA repeat and eight copies of (A)3-6 GT/TG motifs whose function is not known. Each unit of the repeats is 325 bp from the Kansas strain, and 321 pb from the Auburn and Stuttgart strains of channel catfish. The Xba elements are conserved in length within a specific strain, and highly conserved in sequence identity. Sequence identity is more conserved among copies isolated from the same strain than from different strains. The sequence length polymorphism among strains may be useful for identification of strains by polymerase chain reaction analysis. Many features of these elements could make them potentially important for development of homologous recombination expression vectors and position-independent expression vectors.","PeriodicalId":77273,"journal":{"name":"Molecular marine biology and biotechnology","volume":"7 3","pages":"232-9"},"PeriodicalIF":0.0,"publicationDate":"1998-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20617849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Striped bass population subdivision within the santee-cooper system, south carolina 南卡罗来纳santee-cooper系统内条纹鲈鱼种群的细分

Molecular marine biology and biotechnology Pub Date : 1998-09-01

Diaz, Macpherson, Ely, Bulak

引用次数: 0

Usefulness of the medaka beta-actin promoter investigated using a mutant GFP reporter gene in transgenic medaka (Oryzias latipes). 利用突变型GFP报告基因在转基因稻(Oryzias latipes)中研究了稻-肌动蛋白启动子的有效性。

Molecular marine biology and biotechnology Pub Date : 1998-09-01

K Hamada, K Tamaki, T Sasado, Y Watai, S Kani, Y Wakamatsu, K Ozato, M Kinoshita, R Kohno, S Takagi, M Kimura

{"title":"Usefulness of the medaka beta-actin promoter investigated using a mutant GFP reporter gene in transgenic medaka (Oryzias latipes).","authors":"K Hamada, K Tamaki, T Sasado, Y Watai, S Kani, Y Wakamatsu, K Ozato, M Kinoshita, R Kohno, S Takagi, M Kimura","doi":"","DOIUrl":"","url":null,"abstract":"The activity of the medaka beta-actin promoter as a ubiquitous expression vector in transgenic medaka was examined using complementary DNA of the green fluorescent protein (GFP). Plasmid pOBA-GFP contained both the medaka beta-actin promoter and cDNA of the wild-type GFP, while pOBA-hGFP contained the medaka beta-actin promoter and cDNA of the mutant GFP in which serine was substituted for threonine at position 65 and codon usage was humanized to promote translation in vertebrate cells. The ApaI-SmaI fragment of both plasmids was microinjected into the nuclei of oocytes or the cytoplasm of embryos at the one-cell stage. The gene expression was detected, using a fluorescent stereomicroscope, from early stages of development to 1 week after hatching. The expression of the wild-type GFP was detected in early embryos, in the yolk sac and in small portions of the muscle and epidermis. This expression pattern was similar to that of the Escherichia coli beta-galactosidase reporter gene (lacZ), driven by the medaka beta-actin promoter, which was examined in our previous studies. The mutant GFP was expressed in early embryos and in many tissues such as the epidermis, blood vessels, muscle, notochord, fin ray, gut, eyes, and yolk sac, and the fluorescence was much stronger than that of the wild-type GFP. Thus, the usefulness of the medaka beta-actin promoter as a ubiquitous expression vector was confirmed using the mutant GFP as a reporter gene.","PeriodicalId":77273,"journal":{"name":"Molecular marine biology and biotechnology","volume":"7 3","pages":"173-80"},"PeriodicalIF":0.0,"publicationDate":"1998-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20618611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of nuclear proteins interacting with the liver-specific enhancer B element of the antifreeze protein gene in winter flounder. 与冬季比目鱼抗冻蛋白基因特异性增强子B元件相互作用的核蛋白的鉴定。

Molecular marine biology and biotechnology Pub Date : 1998-09-01

M Miao, S L Chan, C L Hew, G L Fletcher

引用次数: 0

Cloning of a cDNA encoding a putative molt-inhibiting hormone from the eyestalk of the sand shrimp Metapenaeus ensis. 沙虾眼柄中一种推测的脱壳抑制激素cDNA的克隆。

Molecular marine biology and biotechnology Pub Date : 1998-09-01

P L Gu, S M Chan

{"title":"Cloning of a cDNA encoding a putative molt-inhibiting hormone from the eyestalk of the sand shrimp Metapenaeus ensis.","authors":"P L Gu, S M Chan","doi":"","DOIUrl":"","url":null,"abstract":"Degenerate primers were designed from the amino acid sequence of the neuropeptide Pej-SGP-IV of the shrimp Penaeus japonicus. Reverse transcriptase-polymerase chain reaction (RT-PCR) was performed using eyestalk complementary DNA of the sand shrimp Metapenaeus ensis. A partial cDNA that codes for a protein homologous to the neuropeptide Pej-SG-IV was cloned. The partial cDNA was used as a probe to screen the eyestalk cDNA library. Several cDNA clones with nucleotide sequence identical to the partial cDNA were isolated. The largest cDNA is 957 bp with an open reading frame consisting of a coding sequence 315 bp in length. The deduced amino acid of the neuropeptide consists of 77 amino acids and is preceded by a signal peptide of 28 amino acids. Because the deduced amino acid sequence of the shrimp cDNA is highly homologous to the Pej-SGP-IV of P. japonicus (which is molt inhibiting) and to other crustaceans' molt-inhibiting hormones (MIHs), the shrimp neuropeptide is tentatively called MeMIH. Northern blot analysis and RT-PCR showed that MeMIH is expressed in the postmolt, intermolt, and premolt stages of the shrimp eyestalks and the brain. Moreover, RNA message can also be detected in the nervous tissues of newly developed larvae. MeMIH is, however, not found in the muscle, swimming leg, and hepatopancreas. Results from genomic Southern blot analysis and amplification of the shrimp genomic DNA by polymerase chain reaction (PCR) suggest that a single copy of the MIH gene is present in the genome. The structural organization of the gene for the shrimp putative MIH is similar to that of the crab Charybdis feriatus.","PeriodicalId":77273,"journal":{"name":"Molecular marine biology and biotechnology","volume":"7 3","pages":"214-20"},"PeriodicalIF":0.0,"publicationDate":"1998-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20617847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation and functional analysis of the histone H3 promoter from atlantic salmon (Salmo salar L.). 大西洋鲑鱼组蛋白H3启动子的分离及功能分析。

Molecular marine biology and biotechnology Pub Date : 1998-09-01

S Hanley, T J Smith, F Muller, N Maclean, S Uzbekova, P Prunet, B Breton

引用次数: 0