German journal of ophthalmology最新文献_第4页

Confocal in vivo microscopy and confocal laser-scanning fluorescence microscopy in keratoconus. 圆锥角膜体内共聚焦显微镜和激光扫描荧光共聚焦显微镜。

German journal of ophthalmology Pub Date : 1996-11-01

S Somodi, C Hahnel, C Slowik, A Richter, D G Weiss, R Guthoff

{"title":"Confocal in vivo microscopy and confocal laser-scanning fluorescence microscopy in keratoconus.","authors":"S Somodi, C Hahnel, C Slowik, A Richter, D G Weiss, R Guthoff","doi":"","DOIUrl":"","url":null,"abstract":"The purpose of this study was the determination of morphological changes in the corneal epithelium and the keratocyte network in keratoconus. In all, 33 eyes of 19 patients were examined in vivo using the confocal slit-scanning microscope Microphthal. After penetrating keratoplasty, recipients' trephanates were stained with the Live/Dead kit and examined using the confocal laser-scanning fluorescence microscope Diaphot 300/Odyssey. The fluorescence images were reconstructed three-dimensionally. All findings were compared with data from healthy corneas. Morphological alterations were found only in the area of the corneal apex; obviously elongated superficial epithelial cells arranged in a whorl-like fashion were found. Near Bowman's membrane, highly reflective changes and fold-like structures were visible. The anterior stroma also showed an increased reflectivity. In the posterior stroma, typical findings were Vogt's striae and keratocytes with extremely long processes arranged nearly in parallel. In scarred stroma the keratocytes were spindle-shaped and arranged irregularly. The spatial organization of the living keratocyte network could be demonstrated through three-dimensional reconstructions.","PeriodicalId":77146,"journal":{"name":"German journal of ophthalmology","volume":"5 6","pages":"518-25"},"PeriodicalIF":0.0,"publicationDate":"1996-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20404738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Photoinduction of fluorophores in the retina using a 514-nm argon ion laser. 使用514纳米氩离子激光在视网膜中光诱导荧光团。

German journal of ophthalmology Pub Date : 1996-11-01

J Inderfurth, G Wagnières

引用次数: 0

Binding of Staphylococcus aureus to fibronectin and glycolipids on corneal surfaces. 金黄色葡萄球菌与角膜表面纤维连接蛋白和糖脂的结合。

German journal of ophthalmology Pub Date : 1996-11-01

U Schwab, H J Thiel, K P Steuhl, G Doering

{"title":"Binding of Staphylococcus aureus to fibronectin and glycolipids on corneal surfaces.","authors":"U Schwab, H J Thiel, K P Steuhl, G Doering","doi":"","DOIUrl":"","url":null,"abstract":"Staphylococcus aureus is one of the most common pathogens of bacterial corneal ulcers. It is generally believed that the first step necessary in the development of an infection involves adherence of bacteria to host tissue. The mechanisms by which staphylococci adhere to ocular epithelium have not yet been defined. An in vitro assay was used to measure binding of S. aureus and Streptococcus pyogenes to a cornea epithelial cell line. Reduction of the amount of cell-surface fibronectin by proteinase treatment decreased the adherence of S. pyogenes to a greater degree than that of S. aureus. The significantly higher number of staphylococci adhering to the cells as compared with S. pyogenes (P < 0.001) suggests the presence of additional binding sites for S. aureus. Using a thin-layer chromatogram overlay assay, we showed binding of S. aureus to various glycolipids extracted from corneal epithelial cells and corneal tissue. S. aureus was found to bind to gangliosides and asialo-GM1, whereas S. pyogenes did not bind to any of these complex lipids. The increased adherence potential of S. aureus due to the ability to bind to glycolipids may provide a selective advantage for S. aureus and explain the prevalence of this organism in bacterial corneal ulcers.","PeriodicalId":77146,"journal":{"name":"German journal of ophthalmology","volume":"5 6","pages":"417-21"},"PeriodicalIF":0.0,"publicationDate":"1996-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20405605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CD 95 expression in traumatic proliferative vitreoretinopathy: a target for the induction of apoptosis. cd95在外伤性增生性玻璃体视网膜病变中的表达:诱导细胞凋亡的靶点。

German journal of ophthalmology Pub Date : 1996-11-01

M Weller, K Heimann, K U Bartz-Schmidt, A Fontana, P Esser

{"title":"CD 95 expression in traumatic proliferative vitreoretinopathy: a target for the induction of apoptosis.","authors":"M Weller, K Heimann, K U Bartz-Schmidt, A Fontana, P Esser","doi":"","DOIUrl":"","url":null,"abstract":"Apoptotic cell death is a central mechanism underlying growth regulation in normal and pathologic neoplastic and nonneoplastic tissue formation. Apoptosis has also been detected in traction membranes of patients with proliferative vitreoretinopathy (PVR). Herein we report that CD 95 (Fas/APO-1), the cell-surface receptor for a potent proapoptotic cytokine, the CD 95 ligand, is expressed in traction membranes of patients with traumatic PVR. Retinal pigment epithelial (RPE) cells, which are thought to contribute to traction membrane formation, express CD 95 in vitro. However, these cells are resistant to CD 95 antibody-induced apoptosis, presumably because they synthesize cytoprotective proteins, which interfere with the CD 95-dependent killing cascade. Coexposure to inhibitors of RNA or protein synthesis sensitizes human RPE cells for CD 95-mediated apoptosis. In contrast, these agents have little effect on the resistance of these cells to tumor necrosis factor-alpha(TNF-alpha). Pre-exposure to TNF-alpha augments CD 95-mediated killing but not its own toxicity in the presence of inhibitors of RNA and protein synthesis. Preexposure to cycloheximide does not abrogate CD 95-mediated apoptosis, suggesting that labile, short-lived proteins do not mediate the cytotoxic effects of CD 95 antibodies. Taken together, our data indicate that immune-mediated CD 95-dependent killing of proliferating cells in vitreoretinal traction membranes might occur in vivo and could possibly be enhanced using novel approaches of immunopharmacotherapy.","PeriodicalId":77146,"journal":{"name":"German journal of ophthalmology","volume":"5 6","pages":"332-7"},"PeriodicalIF":0.0,"publicationDate":"1996-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20404490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Three-dimensional visualization of human cataract in vivo. 人体内白内障的三维可视化。

German journal of ophthalmology Pub Date : 1996-11-01

B R Masters

引用次数: 0

Phacotmesis--a new concept in phacoemulsification. 超声乳化术——超声乳化术的新概念。

German journal of ophthalmology Pub Date : 1996-11-01

J Kammann, G Dornbach

引用次数: 0

Specular microscopy: from speculative to spectacular microscopy. 镜面显微镜:从推测到壮观的显微镜。

German journal of ophthalmology Pub Date : 1996-11-01

C Hartmann, L Bergmann

引用次数: 0

Postoperative ultrasound biomicroscopic evaluation of the haptic position in transsclerally sutured posterior-chamber lenses as compared with the intraoperative endoscopic position. 术后超声生物显微评价经巩膜缝合的后房型晶状体的触觉位置与术中内镜位置的比较。

German journal of ophthalmology Pub Date : 1996-11-01

T Hudde, C Althaus, R Sundmacher

引用次数: 0

Reproducibility of color Doppler imaging in orbital vessels. 眼眶血管彩色多普勒成像的再现性。

German journal of ophthalmology Pub Date : 1996-11-01

B C Senn, H J Kaiser, A Schötzau, J Flammer

{"title":"Reproducibility of color Doppler imaging in orbital vessels.","authors":"B C Senn, H J Kaiser, A Schötzau, J Flammer","doi":"","DOIUrl":"","url":null,"abstract":"The purpose of this study was to evaluate the short-term reproducibility, the long-term reproducibility, and the reproducibility of the measurements made between two different examiners of blood-flow velocity in extraocular vessels using color Doppler imaging (CDI). In a group of 10 healthy volunteers, measurements of the peak-systolic velocity (PSV), end-diastolic velocity (EDV), and resistivity index (RI) in the ophthalmic artery and the central retinal artery as well as one lateral and one medial short posterior ciliary artery were performed. The best intraindividual reproducibility, expressed as the relative error, was found for the PSV and RI measured in the ophthalmic artery (OA; PSV 5.9%, RI 3.1%) and the central retinal artery (CRA; PSV 7.7%, RI 4.7%). The PSV and RI determined in the posterior ciliary arteries were less reproducible but as much so as the EDV measured in the OA (11.8%) and CRA (19.9%). No systematic trend could be found between the first and second measurements. However, the long-term fluctuation was considerable high. Measurements of PSVs showed good concurrence between the two observers in all four vessels measured. The EDVs and RIs differed statistically significantly between the two observers. This difference was most pronounced in the posterior ciliary arteries.","PeriodicalId":77146,"journal":{"name":"German journal of ophthalmology","volume":"5 6","pages":"386-91"},"PeriodicalIF":0.0,"publicationDate":"1996-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20404387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The correlation of corneal refractive power, axial length, and the refractive power of the emmetropizing intraocular lens in cataractous eyes. 白内障眼角膜屈光度、眼轴长度与屈光性人工晶体屈光度的关系。

German journal of ophthalmology Pub Date : 1996-11-01

F N Sayegh

引用次数: 0