Biomaterials, artificial cells, and immobilization biotechnology : official journal of the International Society for Artificial Cells and Immobilization Biotechnology最新文献_第2页

Effect of antigen-specific immunoadsorption on antibody kinetics in a rat model. 抗原特异性免疫吸附对大鼠模型抗体动力学的影响。

Biomaterials, artificial cells, and immobilization biotechnology : official journal of the International Society for Artificial Cells and Immobilization Biotechnology Pub Date : 1993-01-01 DOI: 10.3109/10731199309117357

R A Zeuner, R Béress, J O Schroeder, H H Euler

引用次数: 5

Synthesis and interactions with blood of polyetherurethaneurea/polypeptide block copolymers. 聚醚脲/多肽嵌段共聚物的合成及其与血液的相互作用。

Biomaterials, artificial cells, and immobilization biotechnology : official journal of the International Society for Artificial Cells and Immobilization Biotechnology Pub Date : 1993-01-01 DOI: 10.3109/10731199309117659

Y Ito, K Miyashita, T Kashiwagi, Y Imanishi

引用次数: 4

Enhanced attachment and growth of human endothelial cells derived from umbilical veins on ammonia plasma modified surfaces of PTFE and ePTFE synthetic vascular graft biomaterials. 人脐静脉内皮细胞在氨血浆修饰的PTFE和ePTFE合成血管移植材料表面的附着和生长。

Biomaterials, artificial cells, and immobilization biotechnology : official journal of the International Society for Artificial Cells and Immobilization Biotechnology Pub Date : 1993-01-01 DOI: 10.3109/10731199309117651

R Sipehia, G Martucci, M Barbarosie, C Wu

{"title":"Enhanced attachment and growth of human endothelial cells derived from umbilical veins on ammonia plasma modified surfaces of PTFE and ePTFE synthetic vascular graft biomaterials.","authors":"R Sipehia, G Martucci, M Barbarosie, C Wu","doi":"10.3109/10731199309117651","DOIUrl":"https://doi.org/10.3109/10731199309117651","url":null,"abstract":"Ammonia plasma generated by electrical discharge at low pressure was employed for the surface modification of PTFE and ePTFE. A new chemistry at the plasma treated surfaces is reported. X-ray photoelectron spectroscopy studies showed the incorporation of C-N, C-O, C = O etc functional groups on the plasma treated surfaces. Human endothelial cells derived from umbilical veins (HUEC) were used to seed the plasma treated PTFE and ePTFE surfaces to assess the attachment and growth. Enhanced attachment and growth of HUEC was observed on the plasma treated surfaces. In addition, the performance of these surfaces in this respect was found to be considerably superior to human collagen or human fibronectin or collagen-fibronectin coated PTFE. HUEC attachment and growth on these plasma treated surfaces was further enhanced by immobilizing collagen or fibronectin or collagen-fibronectin. Ammonia plasma treated and untreated ePTFE vascular graft samples were seeded with 3.6 X 10(4) cells/sample. At 24 hrs after seeding, HUEC cell attachment was studied. Although, HUEC attachment on collagen or fibronectin coated ePTFE was improved, but there was no significant difference between the number of cells attached to these surfaces when compared with those adhered to plasma treated ePTFE without collagen or fibronectin coating. Collagen or fibronectin coated plasma treated surfaces showed better performance over their respective controls.","PeriodicalId":77039,"journal":{"name":"Biomaterials, artificial cells, and immobilization biotechnology : official journal of the International Society for Artificial Cells and Immobilization Biotechnology","volume":"21 4","pages":"455-68"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10731199309117651","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19249878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 33

Physiological behaviour of encapsulated somatic embryos. 被包被体细胞胚胎的生理行为。

Biomaterials, artificial cells, and immobilization biotechnology : official journal of the International Society for Artificial Cells and Immobilization Biotechnology Pub Date : 1993-01-01 DOI: 10.3109/10731199309117375

A Kersulec, C Bazinet, F Corbineau, D Côme, J N Barbotin, J F Hervagault, D Thomas

引用次数: 16

A morphological and functional evaluation of transplanted isolated encapsulated hepatocytes following long-term transplantation in Gunn rats. Gunn大鼠肝细胞长期移植后的形态学和功能评价。

Biomaterials, artificial cells, and immobilization biotechnology : official journal of the International Society for Artificial Cells and Immobilization Biotechnology Pub Date : 1993-01-01 DOI: 10.3109/10731199309117351

V Dixit, M Arthur, G Gitnick

{"title":"A morphological and functional evaluation of transplanted isolated encapsulated hepatocytes following long-term transplantation in Gunn rats.","authors":"V Dixit, M Arthur, G Gitnick","doi":"10.3109/10731199309117351","DOIUrl":"https://doi.org/10.3109/10731199309117351","url":null,"abstract":"In this study we investigated the fate of microencapsulated hepatocytes following long-term (6 months) transplantation in Gunn rats. Isolated hepatocytes were microencapsulated with a collagen matrix within an alginate-poly L-lysine composite membrane. Isolated, encapsulated hepatocytes (IEH) or free (unencapsulated) isolated hepatocytes were intraperitoneally transplanted into homozygous Gunn rats that exhibit congenital hyperbilirubinemia. Control Gunn rats received empty microcapsules. Total serum bilirubin was measured at weekly intervals for one month post-IEH transplantation, every two weeks for the next month, and monthly thereafter for up to six months. IEH samples were biopsied from the Gunn rats at monthly intervals and analyzed by light and electron microscopy. A significant (p < 0.01) decrease in total serum bilirubin was observed in IEH transplanted animals during the first month of transplantation. Thereafter, total serum bilirubin levels gradually returned to pre-transplantation levels. A mild, transient decrease in total serum bilirubin was seen in animals transplanted with free (unencapsulated) hepatocytes. No decrease in total serum bilirubin levels was seen in the Gunn rats transplanted with control (empty) microcapsules. Transplanted IEH retained its normal ultrastructure for up to one month and intact microcapsules showed no evidence of hepatocyte rejection, at this time. Degenerative changes observed in the IEH beginning at 2 months post-transplantation, suggests that repeated transplantations may be necessary for long-term effectiveness of IEH therapy.","PeriodicalId":77039,"journal":{"name":"Biomaterials, artificial cells, and immobilization biotechnology : official journal of the International Society for Artificial Cells and Immobilization Biotechnology","volume":"21 2","pages":"119-33"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10731199309117351","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19306558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 27

Cross-linked hemoglobin-superoxide dismutase-catalase scavenges oxygen-derived free radicals and prevents methemoglobin formation and iron release. 交联血红蛋白-超氧化物歧化酶-过氧化氢酶清除氧源自由基，阻止高铁血红蛋白的形成和铁的释放。

Biomaterials, artificial cells, and immobilization biotechnology : official journal of the International Society for Artificial Cells and Immobilization Biotechnology Pub Date : 1993-01-01 DOI: 10.3109/10731199309117385

F D'Agnillo, T M Chang

{"title":"Cross-linked hemoglobin-superoxide dismutase-catalase scavenges oxygen-derived free radicals and prevents methemoglobin formation and iron release.","authors":"F D'Agnillo, T M Chang","doi":"10.3109/10731199309117385","DOIUrl":"https://doi.org/10.3109/10731199309117385","url":null,"abstract":"In this study, we prepared PolyHb-SOD-catalase (intermolecularly cross-linked hemoglobin, superoxide dismutase (SOD), and catalase). We found that PolyHb-SOD-catalase is effective in scavenging oxygen-derived free radicals. In the xanthine/xanthine oxidase system, the initial rate of cytochrome c reduction was 2.13 +/- 0.26 nmoles cyt. c/min for PolyHb alone. PolyHb- SOD-catalase reduced this to 0.56 +/- 0.08 nmoles cyt. c/min because of its ability to eliminate superoxide (O2-). Addition of PolyHb to 200 microM of hydrogen peroxide (H2O2), changed the H2O2 level slightly to 192 +/- 0.4 microM. Addition of PolyHb-SOD-catalase, on the other hand, lower the level to 41 +/- 0.3 microM. Results also show that both effects were dependent on the concentration of SOD-catalase cross-linked with hemoglobin. Oxidative challenge with H2O2 resulted in minimal changes in the absorbance spectra of PolyHb-SOD-catalase. With PolyHb, there were spectral changes reflecting the formation of methemoglobin and heme degradation. Furthermore, the amount of iron released, after incubation with 250 microM H2O2, was 6.8 +/- 1.8 micrograms/dl for PolyHb-SOD-catalase and 76.6 +/- 1.0 micrograms/dl for PolyHb. These results show that cross-linked SOD-catalase prevents oxidative reactions involving the hemoglobin component of PolyHb-SOD-catalase.","PeriodicalId":77039,"journal":{"name":"Biomaterials, artificial cells, and immobilization biotechnology : official journal of the International Society for Artificial Cells and Immobilization Biotechnology","volume":"21 5","pages":"609-21"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10731199309117385","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19110259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 23

New preparation for oral administration of digestive enzyme. Lactase complex microcapsules. 消化酶口服给药新制剂。乳糖酶复合物微胶囊。

Biomaterials, artificial cells, and immobilization biotechnology : official journal of the International Society for Artificial Cells and Immobilization Biotechnology Pub Date : 1993-01-01 DOI: 10.3109/10731199309117388

X L Wang, J Y Shao

{"title":"New preparation for oral administration of digestive enzyme. Lactase complex microcapsules.","authors":"X L Wang, J Y Shao","doi":"10.3109/10731199309117388","DOIUrl":"https://doi.org/10.3109/10731199309117388","url":null,"abstract":"Complex microcapsules which could protect therapeutic enzymes from inactivation in both the stomach and intestine were prepared. Thus, semipermeable microcapsules were first formed by enveloping the enzymes within spherical, ultrathin semipermeable membranes. To resist to the gastric juice, the semipermeable microcapsules were further encapsulated by enteric-soluble materials to form complex microcapsules. When the preparation passes into the intestine, the semipermeable microcapsules are released, thus the small molecules of substrates equilibrate rapidly across the semipermeable membrane to be reacted by the enveloped enzymes, and alimentary proteases remain outside. This complex microencapsulated lactase remained over 65% of its activity after 2 h simulation in gastric juice, and over 65% of its activity was retained after 6 h contact with pancreatin-containing simulated intestinal juice. By contrary, unencapsulated lactase lost immediately all of its activity under similar conditions.","PeriodicalId":77039,"journal":{"name":"Biomaterials, artificial cells, and immobilization biotechnology : official journal of the International Society for Artificial Cells and Immobilization Biotechnology","volume":"21 5","pages":"637-46"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10731199309117388","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19110262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

X-ray photoelectron spectroscopy studies, surface tension measurements, immobilization of human serum albumin, human fibrinogen and human fibronectin onto ammonia plasma treated surfaces of biomaterials useful for cardiovascular implants and artificial cornea implants. x射线光电子能谱研究，表面张力测量，将人血清白蛋白，人纤维蛋白原和人纤维连接蛋白固定在氨血浆处理过的心血管植入物和人工角膜植入物表面。

Biomaterials, artificial cells, and immobilization biotechnology : official journal of the International Society for Artificial Cells and Immobilization Biotechnology Pub Date : 1993-01-01 DOI: 10.3109/10731199309117389

R Sipehia

{"title":"X-ray photoelectron spectroscopy studies, surface tension measurements, immobilization of human serum albumin, human fibrinogen and human fibronectin onto ammonia plasma treated surfaces of biomaterials useful for cardiovascular implants and artificial cornea implants.","authors":"R Sipehia","doi":"10.3109/10731199309117389","DOIUrl":"https://doi.org/10.3109/10731199309117389","url":null,"abstract":"XPS studies of untreated and ammonia plasma treated surfaces of PTFE, ePTFE, Dacron, P(HEMA), PMMA, Silastic and PS were carried out. Ammonia plasma treatment caused significant changes in the surface composition. The curve-fitting results confirmed the incorporation of nitrogen and oxygen in the form of functional groups such as C-N, C=O, C-O, Si-N, Si-OH etc. Increases in the values of surface tension occurred. The surface tension of plasma treated surfaces varied between 44-48 erg/cm2 with the exception of Dacron which became wettable. Enhanced immobilization of human albumin on plasma treated surfaces was achieved. When washed with 0.2% Tween in buffer, these albuminated surfaces were found to be stable compared to control samples. Increased immobilization of human fibrinogen was also observed. The ammonia plasma treated surfaces showed high binding properties and retention for human fibronectin. Ionic interaction between proteins solution and plasma treated surfaces may be cause of the increase attachment of these biological molecules.","PeriodicalId":77039,"journal":{"name":"Biomaterials, artificial cells, and immobilization biotechnology : official journal of the International Society for Artificial Cells and Immobilization Biotechnology","volume":"21 5","pages":"647-58"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10731199309117389","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19110263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 19

Characterization of rifamycin oxidase immobilized on alginate gel. 海藻酸盐凝胶固定化利福霉素氧化酶的研究。

Biomaterials, artificial cells, and immobilization biotechnology : official journal of the International Society for Artificial Cells and Immobilization Biotechnology Pub Date : 1993-01-01 DOI: 10.3109/10731199309117392

U C Banerjee

引用次数: 1

Free and microencapsulated Erwinia herbicola for the production of tyrosine. 游离和微囊化Erwinia除草剂用于生产酪氨酸。

Biomaterials, artificial cells, and immobilization biotechnology : official journal of the International Society for Artificial Cells and Immobilization Biotechnology Pub Date : 1993-01-01 DOI: 10.3109/10731199309117370

I Lloyd-George, T M Chang

{"title":"Free and microencapsulated Erwinia herbicola for the production of tyrosine.","authors":"I Lloyd-George, T M Chang","doi":"10.3109/10731199309117370","DOIUrl":"https://doi.org/10.3109/10731199309117370","url":null,"abstract":"Erwinia herbicola (ATCC 21434) was grown in a medium which caused the cells to induce tyrosine phenol-lyase (TPL) activity. Whole cells of Erwinia herbicola were then microencapsulated within alginate-poly-L-lysine-alginate membraned microcapsules (diameter 800 microns). In a rotary shaker-incubator with a 1.9 cm horizontal throw, an agitation rate of at least 240 revolutions per minute (rpm) was required before the TPL activity of the microencapsulated cells was equal to that of the free cells. The TPL activity of the cells, whether free or microencapsulated, could be used for the conversion of ammonia, pyruvate and phenol into tyrosine at 37 degrees C. The results indicate that free cells and microencapsulated cells effect the conversion of these reactants to tyrosine equally well if the agitation rate is 240 rpm. In liver failure the concentrations of both ammonia, phenol and pyruvate are elevated. Hence the TPL activity of microencapsulated Erwinia herbicola could possibly find application in a novel approach for the removal of toxic phenol and ammonia during liver failure.","PeriodicalId":77039,"journal":{"name":"Biomaterials, artificial cells, and immobilization biotechnology : official journal of the International Society for Artificial Cells and Immobilization Biotechnology","volume":"21 3","pages":"323-33"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10731199309117370","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19383885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11