Biomaterials, artificial cells, and immobilization biotechnology : official journal of the International Society for Artificial Cells and Immobilization Biotechnology最新文献_第10页

Dehydration and shock: an animal model of hemorrhage and resuscitation of battlefield injury. 脱水与休克:战场创伤出血与复苏动物模型。

Biomaterials, artificial cells, and immobilization biotechnology : official journal of the International Society for Artificial Cells and Immobilization Biotechnology Pub Date : 1992-01-01 DOI: 10.3109/10731199209119675

J R Hess, V W MacDonald, R M Winslow

{"title":"Dehydration and shock: an animal model of hemorrhage and resuscitation of battlefield injury.","authors":"J R Hess, V W MacDonald, R M Winslow","doi":"10.3109/10731199209119675","DOIUrl":"https://doi.org/10.3109/10731199209119675","url":null,"abstract":"We have developed a porcine model of the anticipated military use of oxygen-carrying resuscitation solutions. The objective is to determine whether toxicity under adverse conditions will limit further development of hemoglobin-based products. Splenectomized immature female swine are used because of their extensive use in the evaluation of other resuscitation solutions. Five days prior to each experiment, central vascular catheters and a renal arterial flow probe are surgically placed in the animals. After recovery and weight gain has resumed, animals are placed in metabolic cages and deprived of water for 48 hours to produce hyperosmolar dehydration resulting in loss of approximately 7% of body weight. We remove 38% of estimated blood volume, 25 ml/kg, over one hour by a controlled logarithmic hemorrhage. Resuscitation is by administration of a fixed volume of test solution. Hemodynamic function is observed but not further therapy is given for three hours, a period corresponding to evacuation in the field. After this period, corresponding to arrival at a field hospital, the animals' blood is returned. Swine are then observed in metabolic cages for an additional 7 days while blood and urine are sampled daily. At the end of this period, animals are anesthetized, urinary catheters are implanted, and creatinine clearances are measured. Swine are than euthanized, and their tissues are examined. In a pilot study, resuscitation was performed with either Ringer's lactate, albumin, stroma-free hemoglobin, or cross-linked (alpha alpha Hb) hemoglobin. All animals survived.","PeriodicalId":77039,"journal":{"name":"Biomaterials, artificial cells, and immobilization biotechnology : official journal of the International Society for Artificial Cells and Immobilization Biotechnology","volume":"20 2-4","pages":"499-502"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10731199209119675","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12563517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 28

Telomeric THAM-derived perfluoroalkylated surfactants for fluorocarbon emulsions. 端粒tham衍生的全氟烷基表面活性剂，用于氟碳乳液。

Biomaterials, artificial cells, and immobilization biotechnology : official journal of the International Society for Artificial Cells and Immobilization Biotechnology Pub Date : 1992-01-01 DOI: 10.3109/10731199209119727

J G Riess, A A Pavia, B Pucci, L Zarif

引用次数: 2

Effects of hypothermic conditions on the oxygen carrying capacity of crosslinked hemoglobins. 低温条件对交联血红蛋白携氧能力的影响。

Biomaterials, artificial cells, and immobilization biotechnology : official journal of the International Society for Artificial Cells and Immobilization Biotechnology Pub Date : 1992-01-01 DOI: 10.3109/10731199209119641

A I Alayash, J C Frantantoni

{"title":"Effects of hypothermic conditions on the oxygen carrying capacity of crosslinked hemoglobins.","authors":"A I Alayash, J C Frantantoni","doi":"10.3109/10731199209119641","DOIUrl":"https://doi.org/10.3109/10731199209119641","url":null,"abstract":"In view of the potential application for hemoglobin-based oxygen carriers (HBOCs) in organ perfusion under hypothermic conditions, we examined the temperature dependence of oxygen equilibrium curves (OECs) at 15-37 degrees C of three HBOCs: HbA-FMDA and HbBv-FMDA, produced by the reaction of human or bovine oxyHb with fumaryl mono-dibromoaspirin, and HbA-DBBF, produced by the reaction of human deoxyHb with bis(3,5-dibromosalicyl) fumarate. OECs for HbA-DBBF, HbA-FMDA and HbBv-FMDA at 37 degrees C were right shifted (P50 = 24.5, 17 and 35 torr, respectively). van't Hoff's rule gave HbA-DBBF (-12.2 +/- 2.8), HbA-FMDA (-12.0 +/- 2.0), HbBv-FMDA (-10.5 +/- 1.8); these values do not significantly differ from that for native HbAo (-11.5 +/- 2.4). Among the hemoglobins included in this study, HbBv-FMDA had the most favorable oxygenation characteristics at low temperatures (a P50 of 6.0 torr at 15 degrees C as compared to only 2-3 torr for the other hemoglobins in the study). Recently, however, a human hemoglobin crosslinked with bis-pyridoxyl tetraphosphate was reported to have a P50 of 15 torr at 16 degrees C (Keipert et al, Transfusion 1989; 29: 768-773). Therefore, precise knowledge of the oxygen delivering capacity of any potential HBOC should be explored under hypothermic conditions as performance under these conditions may determine its usefulness as an organ perfusate.","PeriodicalId":77039,"journal":{"name":"Biomaterials, artificial cells, and immobilization biotechnology : official journal of the International Society for Artificial Cells and Immobilization Biotechnology","volume":"20 2-4","pages":"259-62"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10731199209119641","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12563813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Hemoglobin-based oxygen carriers (HBOCs): structural alterations that affect free radical generation. 血红蛋白基氧载体(HBOCs):影响自由基生成的结构改变。

Biomaterials, artificial cells, and immobilization biotechnology : official journal of the International Society for Artificial Cells and Immobilization Biotechnology Pub Date : 1992-01-01 DOI: 10.3109/10731199209119643

A I Alayash, B A Ryan, J C Fratantoni, J Bonaventura, C Bonaventura

{"title":"Hemoglobin-based oxygen carriers (HBOCs): structural alterations that affect free radical generation.","authors":"A I Alayash, B A Ryan, J C Fratantoni, J Bonaventura, C Bonaventura","doi":"10.3109/10731199209119643","DOIUrl":"https://doi.org/10.3109/10731199209119643","url":null,"abstract":"We examined how changes in oxygen affinity brought about by different chemical modifications of hemoglobins affect their oxidation-reduction reactions. The three modified hemoglobins studied were HbA-FMDA, HbBv-FMDA, produced by the reaction of human or bovine oxyHb with fumaryl mono-dibromoaspirin; and HbA-DBBF, produced by the reaction of human deoxyHb with bis(3,5-dibromosalicyl) fumarate. Exposure of oxyHb to H2O2 causes generation of free radicals capable of cleaving dimethylsulfoxide (Me2SO) to produce formaldehyde (HCHO). Relative to the reaction rate for HbAo (630 +/- 130 M/min) the rates of HCHO formation were roughly 70% for HbA-DBBF, 50% for HbA-FMDA and 16% for HbBv-FMDA. Exposure to H2O2 also caused spectral changes at varied rates for the HBOCs analyzed. Although these rates were not directly correlated with the rates of free radical formation, addition of mannitol or thiourea slowed both the rate of spectral changes and HCHO formation. The relative ability of the ferric derivatives of the HBOCs to participate in free radical reactions was monitored by assays of non-enzymatic NADPH oxidation and aniline hydroxylation. HbBv-FMDA showed significantly slower rates than the other HBOCs in both assays. The observed differences between HBOCs in these assays indicate differences in their ability to generate or interact with free radicals.","PeriodicalId":77039,"journal":{"name":"Biomaterials, artificial cells, and immobilization biotechnology : official journal of the International Society for Artificial Cells and Immobilization Biotechnology","volume":"20 2-4","pages":"277-81"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10731199209119643","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12563815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Clearance of differentially labelled infused hemoglobin and polymerized hemoglobin from dog plasma and accumulation in urine and selected tissues. 清除犬血浆中的差异标记输注血红蛋白和聚合血红蛋白，以及尿液和选定组织中的积累。

Biomaterials, artificial cells, and immobilization biotechnology : official journal of the International Society for Artificial Cells and Immobilization Biotechnology Pub Date : 1992-01-01 DOI: 10.3109/10731199209119719

P J Anderson, J Ning, G P Biro

{"title":"Clearance of differentially labelled infused hemoglobin and polymerized hemoglobin from dog plasma and accumulation in urine and selected tissues.","authors":"P J Anderson, J Ning, G P Biro","doi":"10.3109/10731199209119719","DOIUrl":"https://doi.org/10.3109/10731199209119719","url":null,"abstract":"Pyridoxalated hemoglobin polymerized with glutaraldehyde has been proposed as a hemoglobin based blood substitute. The preparations contain significant amounts of unpolymerized hemoglobin. We have prepared polymerized pyridoxalated hemoglobin labelled with 14C by reductive methylation free of unpolymerized hemoglobin and pyridoxalated hemoglobin labelled with 3H by reductive methylation to compare the handling of the two forms after infusion into dogs. Four dogs were examined sequentially. After three hours, 52.4 +/- 8.9% of the 3H label had disappeared from plasma whereas 21.7 +/- 5.8 of the 14C label had disappeared. The decrease of both labels occurred in a very close to linear fashion over the time period examined. From radioactivity in collected urine, it was calculated that 30.7 +/- 6.3% of the 3H and 9.0 +/- 2.7 of the 14C that had been cleared from plasma appeared in urine. The ratio of the specific radioactivity in tissue to the specific radioactivity of plasma indicated that extravascular accumulation of 3H label from unpolymerized hemoglobin occurred in kidney, heart and liver, with the kidney cortex exhibiting a very high concentration of the label. The specific radioactivity of both 3H and 14C label in liver suggested the substantial involvement of the reticuloendothelial system in the removal of both unpolymerized and polymerized hemoglobin from the circulation.","PeriodicalId":77039,"journal":{"name":"Biomaterials, artificial cells, and immobilization biotechnology : official journal of the International Society for Artificial Cells and Immobilization Biotechnology","volume":"20 2-4","pages":"781-7"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10731199209119719","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12563823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Some aspects of perfluorochemical emulsion's interaction with blood. 全氟化学乳剂与血液相互作用的几个方面。

Biomaterials, artificial cells, and immobilization biotechnology : official journal of the International Society for Artificial Cells and Immobilization Biotechnology Pub Date : 1992-01-01 DOI: 10.3109/10731199209119754

E V Tereshina, N N Doronina, N I Afonin, I N Ozerova, I A Tcherbacova

引用次数: 2

Effects of intravenous infusions of diaspirin cross-linked hemoglobin (DCLHb) on sheep. 静脉输注双阿司匹林交联血红蛋白(DCLHb)对绵羊的影响。

Biomaterials, artificial cells, and immobilization biotechnology : official journal of the International Society for Artificial Cells and Immobilization Biotechnology Pub Date : 1992-01-01 DOI: 10.3109/10731199209119686

K E Burhop, L Farrell, C Nigro, D Tan, T Estep

{"title":"Effects of intravenous infusions of diaspirin cross-linked hemoglobin (DCLHb) on sheep.","authors":"K E Burhop, L Farrell, C Nigro, D Tan, T Estep","doi":"10.3109/10731199209119686","DOIUrl":"https://doi.org/10.3109/10731199209119686","url":null,"abstract":"The purpose of this study was to compare the cardiopulmonary, hematologic, and immunologic responses of unanesthetized sheep to single, \"topload\", intravenous infusions of either 10 mL/Kg or 40 mL/Kg of Diaspirin Cross-Linked Hemoglobin, 10 mL/Kg or 40 mL/Kg of a Human Serum Albumin (HSA) solution oncotically adjusted with human serum albumin to approximately match the oncotic pressure of the DCLHb, or 10 mL/Kg of Erythrocyte Hemolysate solution prepared in a manner similar to that commonly described in the literature and referred to as \"stroma free hemoglobin\". Solutions were infused at a rate of 1 mL/Kg/minute and animals were monitored for 72 hours after infusion. These studies demonstrated that in sheep infusion of either DCLHb or HSA solutions was well tolerated and did not produce a significant increase in plasma C3a levels, an increase in the plasma concentration of thromboxane B2, or unexpected fluid shifts. In contrast, infusion of the Erythrocyte Hemolysate produced a greater than 10-fold increase in plasma C3a concentrations, a greater than 6000-fold increase in plasma TxB2 concentration, significant fluid shifts, and changes in a variety of other parameters consistent with induction of a dramatic inflammatory response. These results indicate that appropriately prepared and purified DCLHb solutions do not elicit an inflammatory reaction in sheep.","PeriodicalId":77039,"journal":{"name":"Biomaterials, artificial cells, and immobilization biotechnology : official journal of the International Society for Artificial Cells and Immobilization Biotechnology","volume":"20 2-4","pages":"581-5"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10731199209119686","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12563979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 24

Immunogenicity of diaspirin cross-linked human hemoglobin solutions. 双阿司匹林交联人血红蛋白溶液的免疫原性。

Biomaterials, artificial cells, and immobilization biotechnology : official journal of the International Society for Artificial Cells and Immobilization Biotechnology Pub Date : 1992-01-01 DOI: 10.3109/10731199209119689

T N Estep, J Gonder, I Bornstein, F Aono

引用次数: 21

Effects of modified hemoglobin solutions on the isolated rabbit heart. 改性血红蛋白溶液对离体兔心脏的影响。

Biomaterials, artificial cells, and immobilization biotechnology : official journal of the International Society for Artificial Cells and Immobilization Biotechnology Pub Date : 1992-01-01 DOI: 10.3109/10731199209119704

P T Biessels, J B Hak, W K Bleeker, J H van Beek, J C Bakker

{"title":"Effects of modified hemoglobin solutions on the isolated rabbit heart.","authors":"P T Biessels, J B Hak, W K Bleeker, J H van Beek, J C Bakker","doi":"10.3109/10731199209119704","DOIUrl":"https://doi.org/10.3109/10731199209119704","url":null,"abstract":"The effects of modified hemoglobin (Hb) solutions on the coronary vasculature were studied. Hearts were perfused according to Langendorff with constant flow of Tyrode solution. The solutions studied were stroma- free Hb, prepared by lysis of red blood cells in water (SFHb-lys), or prepared by swelling of red blood cells in hypotonic phosphate buffer (SFHb). The increase in coronary vascular resistance at a dose of 200 mg Hb/dl was 68% for SFHb-lys and 13% for SFHb, respectively. Addition of the modified Hb solutions HbNFPLP and polyHbNFPLP produced an increase in coronary resistance of 11% and 8%, respectively. The left ventricular developed pressure (LVDP) (control value 72 +/- 12 mm Hg) increased by 18 and 12 mm Hg, respectively, for a dose of 250 mg Hb/dl. When HbNFPLP was converted to its met-Hb form the increase in LVDP was reduced to 3 mmHg and the increase in perfusion pressure to 6 mm Hg. We conclude that elimination of stromal contamination from Hb solutions can diminish vasoconstrictor effects. The increase in cardiac pressure development and in coronary vascular resistance found for dilute modified Hb solutions is partly due to an improved oxygen transport to the heart.","PeriodicalId":77039,"journal":{"name":"Biomaterials, artificial cells, and immobilization biotechnology : official journal of the International Society for Artificial Cells and Immobilization Biotechnology","volume":"20 2-4","pages":"693-6"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10731199209119704","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12564533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Effects of pyridoxalated hemoglobin polyoxyethylene conjugate(PHP) on renal circulation in isolated perfused rat kidneys. 吡哆醛化血红蛋白聚氧乙烯偶联物(PHP)对离体灌注大鼠肾脏循环的影响。

Biomaterials, artificial cells, and immobilization biotechnology : official journal of the International Society for Artificial Cells and Immobilization Biotechnology Pub Date : 1992-01-01 DOI: 10.3109/10731199209119710

K Nishi, S Ueda, K Nishi

引用次数: 3