Applied and theoretical electrophoresis : the official journal of the International Electrophoresis Society最新文献_第9页

Three electrophoretic techniques comparison for des-gamma-carboxyprothrombin detection. 三种电泳技术检测脱氧-羧基凝血酶原的比较。

Applied and theoretical electrophoresis : the official journal of the International Electrophoresis Society Pub Date : 1992-01-01

M Belle, M Guillaumont, M Leclercq, R Guinet

引用次数: 0

Virulence associated proteins of Brucella abortus identified by paired two-dimensional gel electrophoretic comparisons of virulent, vaccine and LPS deficient strains. 流产布鲁氏菌毒力相关蛋白的鉴定，通过对二维凝胶电泳的毒性比较，疫苗和LPS缺陷菌株。

Applied and theoretical electrophoresis : the official journal of the International Electrophoresis Society Pub Date : 1992-01-01

B A Sowa, K A Kelly, T A Ficht, L G Adams

{"title":"Virulence associated proteins of Brucella abortus identified by paired two-dimensional gel electrophoretic comparisons of virulent, vaccine and LPS deficient strains.","authors":"B A Sowa, K A Kelly, T A Ficht, L G Adams","doi":"","DOIUrl":"","url":null,"abstract":"To identify molecular determinants of virulence, the proteins of Brucella abortus strains 2308 (virulent), S19 (vaccine) and lipopolysaccharide deficient rough mutants derived from each (RB51 and S19M3 respectively) were compared by 2-D gel electrophoresis. A total of 996 proteins were identified on autoradiographs of 2-D gels containing [35S]-labeled proteins from these four strains. Proteins differing qualitatively or quantitatively (greater than or equal to 10X) between 2308 and S19 are implicated in virulence and are identified by Mr and pI. Paired comparisons of proteins present in both 2308 and RB51 and missing in both S19 and M3 were used to make tentative identification of 14 putative virulence proteins representing primary expression of genetic differences between virulent and vaccine strains. 28 proteins and/or core lipopolysaccharide-protein complexes involved in the biosynthesis of lipopolysaccharide were identified by paired comparisons of proteins present in both smooth strains and missing in both rough strains.","PeriodicalId":77007,"journal":{"name":"Applied and theoretical electrophoresis : the official journal of the International Electrophoresis Society","volume":"3 1","pages":"33-40"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12767072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Monoclonal gammopathy in a 30 weeks old premature infant. 单克隆γ病在30周龄早产儿。

Applied and theoretical electrophoresis : the official journal of the International Electrophoresis Society Pub Date : 1992-01-01

J D Tissot, P Schneider, P Hohlfeld, J F Tolsa, A Calame, D F Hochstrasser

引用次数: 0

Red blood cell protein map: a comparison between carrier-ampholyte pH gradient and immobilized pH gradient, and identification of four red blood cell enzymes. 红细胞蛋白图谱:载体-两性电解质pH梯度与固定化pH梯度的比较，四种红细胞酶的鉴定。

Applied and theoretical electrophoresis : the official journal of the International Electrophoresis Society Pub Date : 1992-01-01

O G Golaz, C Walzer, D Hochstrasser, B Bjellqvist, H Turler, L Balant

引用次数: 0

Restriction fragment length polymorphism analysis of zoo animals using HaeIII and four single-locus probes. 利用HaeIII和4个单位点探针分析动物园动物限制性内切片段长度多态性。

Applied and theoretical electrophoresis : the official journal of the International Electrophoresis Society Pub Date : 1992-01-01

S M Watson, J D Ban, G T Duncan, K E Noppinger, D J Saxner

{"title":"Restriction fragment length polymorphism analysis of zoo animals using HaeIII and four single-locus probes.","authors":"S M Watson, J D Ban, G T Duncan, K E Noppinger, D J Saxner","doi":"","DOIUrl":"","url":null,"abstract":"Using HaeIII as the restriction endonuclease, restriction fragment length polymorphism analysis of dried blood samples from various animals was conducted. Single-locus probes D2S44, D10S28, D1S7, and D4S139, as well as monomorphic probe D7Z2, were used to examine for banding patterns. If bands were present, the samples were further examined for heterogeneity (whether single or multiple bands were observed) and polymorphism (whether variation in band location was shown between the animals studied within a species). Blood samples from animals, including primates, were obtained from Miami Metrozoo, Miami, Florida. Some of the animals were non-related individuals while others were related. Banding patterns were observed in colobus' for D2S44, D1S7, and D4S139; owl monkeys for D2S44; gorillas for D2S44 and D4S139; gibbons for D2S44 and D4S139; siamangs for D2S44, talapoins for DiS7; cranes for D1S7; and otters for D1S7. Based upon these, all of the animals for which a conclusion could be drawn appeared to be homozygous and monomorphic (exhibited only an invariant single band) for the loci examined except colobus' for D4S139, gorillas for D4S139, cranes for D1S7, and otters for D1S7.","PeriodicalId":77007,"journal":{"name":"Applied and theoretical electrophoresis : the official journal of the International Electrophoresis Society","volume":"3 2","pages":"109-14"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12534187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrophoresis and silver staining of rat urinary proteins including alpha 2u-globulin (rat n II). 大鼠尿蛋白包括α - u-球蛋白(大鼠nⅱ)的电泳和银染色。

Applied and theoretical electrophoresis : the official journal of the International Electrophoresis Society Pub Date : 1992-01-01

T Marshall, K M Williams, C Bayard, O Vesterberg

引用次数: 0

Effect of operating variables on the separation of DNA molecules by capillary polyacrylamide gel electrophoresis. 操作变量对毛细管聚丙烯酰胺凝胶电泳分离DNA分子的影响。

Applied and theoretical electrophoresis : the official journal of the International Electrophoresis Society Pub Date : 1992-01-01

A Guttman

引用次数: 0

The use of fluorescence detection and internal lane standards to size PCR products automatically. 利用荧光检测和内巷标准自动测定PCR产物的大小。

Applied and theoretical electrophoresis : the official journal of the International Electrophoresis Society Pub Date : 1992-01-01

P E Mayrand, K P Corcoran, J S Ziegle, J M Robertson, L B Hoff, M N Kronick

引用次数: 0

Two dimensional gel electrophoresis of cellular and secreted proteins from rat alveolar macrophages after lipopolysaccharide treatment. 脂多糖处理后大鼠肺泡巨噬细胞细胞和分泌蛋白的二维凝胶电泳。

Applied and theoretical electrophoresis : the official journal of the International Electrophoresis Society Pub Date : 1992-01-01

B A Merrick, C Y He, W A Craig, G C Clark, E Corsini, G J Rosenthal, B K Mansfield, J K Selkirk

{"title":"Two dimensional gel electrophoresis of cellular and secreted proteins from rat alveolar macrophages after lipopolysaccharide treatment.","authors":"B A Merrick, C Y He, W A Craig, G C Clark, E Corsini, G J Rosenthal, B K Mansfield, J K Selkirk","doi":"","DOIUrl":"","url":null,"abstract":"Two dimensional gel electrophoresis (2-D PAGE) and automated image analysis were used to study the effects of bacterial lipopolysaccharide (LPS) activation on secreted and cellular rat alveolar macrophage proteins. Primary alveolar macrophages were cultured and exposed to LPS in the presence of [35S]-methionine for 24 h. Image analysis of 2-D PAGE revealed that LPS treatment primarily modulated the proportions of several alveolar macrophage cellular proteins versus control in addition to limited protein induction and repression. The differential effect of LPS was more pronounced on secreted proteins where qualitative and quantitative differences from control cells were found. Immunoblots of secreted proteins with anti-tumor necrosis factor alpha (TNF alpha) and anti-interleukin-1 alpha(IL-1 alpha) antibodies identified these monokines from protein fluorographic patterns. IL-1 alpha was detected as a single polypeptide of 17 kD at pI = 5. Use of recombinant TNF alpha and monoclonal and polyclonal antibodies for immunodetection revealed the 17 kD form of TNF alpha as well as higher molecular weight species at 18 kD and 22 kD. Thus, analysis of radiolabeled rat macrophages treated with LPS reveals quantitative modulation of several cellular proteins as well as a distinctive pattern of secreted proteins which contain multiple monokine forms resolvable by 2-D PAGE.","PeriodicalId":77007,"journal":{"name":"Applied and theoretical electrophoresis : the official journal of the International Electrophoresis Society","volume":"2 6","pages":"177-87"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12735573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Two-dimensional gel analysis of serum apolipoprotein A-I isoforms: preliminary analysis suggests altered ratios in individuals with heart disease. 血清载脂蛋白A-I亚型的二维凝胶分析:初步分析表明心脏病患者的比率改变。

Applied and theoretical electrophoresis : the official journal of the International Electrophoresis Society Pub Date : 1992-01-01

A Burgess-Cassler, J J Johansen, N C Kendrick

{"title":"Two-dimensional gel analysis of serum apolipoprotein A-I isoforms: preliminary analysis suggests altered ratios in individuals with heart disease.","authors":"A Burgess-Cassler, J J Johansen, N C Kendrick","doi":"","DOIUrl":"","url":null,"abstract":"Apolipoprotein A-I (apo A-I), the major protein component of high-density lipoprotein, is quantified in a number of ways, typically by immunochemical methods. Commercial tests do not discriminate among isoforms of apo A-I. Two-dimensional electrophoresis, however, segregates and differentiates these isoforms, primarily due to charge variations among the various species. Stained two-dimensional gels can be scanned using high-resolution laser densitometry, and the isoforms can then be quantified using image analysis software. Human sera from coronary heart disease (CHD) patients (n = 36) and sex-matched and close-age-matched individuals (n = 36) with no known CHD were analyzed, to determine the relative abundance for each isoform within a given serum. In this preliminary study, we observed statistically significant differences between the two groups, suggesting altered post-translational processing from the proapo A-I and mature apo A-I isoforms to their adjacent isoforms for patients with histories of heart disease. In two instances, the P values were less than 0.005; in two others, P values were less than 0.001.","PeriodicalId":77007,"journal":{"name":"Applied and theoretical electrophoresis : the official journal of the International Electrophoresis Society","volume":"3 1","pages":"41-5"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12767073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0