{"title":"Three electrophoretic techniques comparison for des-gamma-carboxyprothrombin detection.","authors":"M Belle, M Guillaumont, M Leclercq, R Guinet","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>Des-gamma-carboxyprothrombin (DCP) is a marker that appears in the blood when modifications of vitamin K-dependent proteins carboxylation cycle occur. About 280 human plasma samples of diverse origins were tested by three different electrophoretic techniques for the evaluation of DCP: rocket immunoelectrophoresis (RIE) before and after barium carbonate adsorption, crossed affinoimmunoelectrophoresis (CAIE) and polyacrylamide gel electrophoresis in presence of calcium lactate followed by immunoblotting (PAGE-blot). A good correlation was found between CAIE and PAGE-blot in the CAIE detection limit, but not between RIE and the two other techniques. PAGE-blot was more sensitive than RIE and CAIE and allowed reliable quantification of abnormal prothrombin in plasma.</p>","PeriodicalId":77007,"journal":{"name":"Applied and theoretical electrophoresis : the official journal of the International Electrophoresis Society","volume":"3 2","pages":"73-6"},"PeriodicalIF":0.0000,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Applied and theoretical electrophoresis : the official journal of the International Electrophoresis Society","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Des-gamma-carboxyprothrombin (DCP) is a marker that appears in the blood when modifications of vitamin K-dependent proteins carboxylation cycle occur. About 280 human plasma samples of diverse origins were tested by three different electrophoretic techniques for the evaluation of DCP: rocket immunoelectrophoresis (RIE) before and after barium carbonate adsorption, crossed affinoimmunoelectrophoresis (CAIE) and polyacrylamide gel electrophoresis in presence of calcium lactate followed by immunoblotting (PAGE-blot). A good correlation was found between CAIE and PAGE-blot in the CAIE detection limit, but not between RIE and the two other techniques. PAGE-blot was more sensitive than RIE and CAIE and allowed reliable quantification of abnormal prothrombin in plasma.
des -羧基凝血酶原(DCP)是维生素k依赖性蛋白羧基化循环发生改变时出现在血液中的标记物。采用三种不同的电泳技术对280个不同来源的人血浆样品进行了测试，以评估DCP:碳酸钡吸附前后的火箭免疫电泳(RIE)，交叉affinoimmunoelectrophoresis (CAIE)和乳酸钙存在下的聚丙烯酰胺凝胶电泳，然后进行免疫印迹(PAGE-blot)。在cae的检出限上，与PAGE-blot有良好的相关性，而与其他两种技术无相关性。PAGE-blot比RIE和cae更敏感，可以可靠地定量血浆中异常的凝血酶原。
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