大鼠尿蛋白包括α - u-球蛋白(大鼠nⅱ)的电泳和银染色。

Applied and theoretical electrophoresis : the official journal of the International Electrophoresis Society Pub Date : 1992-01-01

T Marshall, K M Williams, C Bayard, O Vesterberg

{"title":"大鼠尿蛋白包括α - u-球蛋白(大鼠nⅱ)的电泳和银染色。","authors":"T Marshall, K M Williams, C Bayard, O Vesterberg","doi":"","DOIUrl":null,"url":null,"abstract":"Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and silver staining have been used to monitor the fractionation of rat urinary proteins by Amicon Centriprep C30/C10 ultrafiltration and to assess the purification of alpha 2u-globulin, a laboratory animal allergen, precipitated by exhaustive dialysis. Factors influencing the protein composition of rat urine have been identified. Dialysis procedures have been evaluated for precipitation of alpha 2u-globulin and ultrafiltration used for further purification of the allergen.","PeriodicalId":77007,"journal":{"name":"Applied and theoretical electrophoresis : the official journal of the International Electrophoresis Society","volume":"2 6","pages":"189-92"},"PeriodicalIF":0.0000,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Electrophoresis and silver staining of rat urinary proteins including alpha 2u-globulin (rat n II).\",\"authors\":\"T Marshall, K M Williams, C Bayard, O Vesterberg\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and silver staining have been used to monitor the fractionation of rat urinary proteins by Amicon Centriprep C30/C10 ultrafiltration and to assess the purification of alpha 2u-globulin, a laboratory animal allergen, precipitated by exhaustive dialysis. Factors influencing the protein composition of rat urine have been identified. Dialysis procedures have been evaluated for precipitation of alpha 2u-globulin and ultrafiltration used for further purification of the allergen.\",\"PeriodicalId\":77007,\"journal\":{\"name\":\"Applied and theoretical electrophoresis : the official journal of the International Electrophoresis Society\",\"volume\":\"2 6\",\"pages\":\"189-92\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1992-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Applied and theoretical electrophoresis : the official journal of the International Electrophoresis Society\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Applied and theoretical electrophoresis : the official journal of the International Electrophoresis Society","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 0

摘要

采用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和银染色监测Amicon Centriprep C30/C10超滤对大鼠尿蛋白的分离，并评估用彻底透析沉淀的实验动物过敏原α - u-球蛋白的纯化效果。影响大鼠尿液蛋白质组成的因素已经确定。透析方法已被评估为α - u-球蛋白的沉淀和超滤用于进一步纯化过敏原。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

本刊更多论文

Electrophoresis and silver staining of rat urinary proteins including alpha 2u-globulin (rat n II).

Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and silver staining have been used to monitor the fractionation of rat urinary proteins by Amicon Centriprep C30/C10 ultrafiltration and to assess the purification of alpha 2u-globulin, a laboratory animal allergen, precipitated by exhaustive dialysis. Factors influencing the protein composition of rat urine have been identified. Dialysis procedures have been evaluated for precipitation of alpha 2u-globulin and ultrafiltration used for further purification of the allergen.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Applied and theoretical electrophoresis : the official journal of the International Electrophoresis Society

自引率

0.00%

发文量