Applied and theoretical electrophoresis : the official journal of the International Electrophoresis Society最新文献_第6页

Complement C3 fragments in urine: detection in systemic lupus erythematosus patients by western blotting. 免疫印迹法检测系统性红斑狼疮患者尿液补体C3片段。

Applied and theoretical electrophoresis : the official journal of the International Electrophoresis Society Pub Date : 1993-01-01

R H Kelly, A B Carpenter, K S Sudol, S P Jagarlapudi, S Manzi

{"title":"Complement C3 fragments in urine: detection in systemic lupus erythematosus patients by western blotting.","authors":"R H Kelly, A B Carpenter, K S Sudol, S P Jagarlapudi, S Manzi","doi":"","DOIUrl":"","url":null,"abstract":"We characterized urinary excretion of C3 fragments among patients with systemic lupus erythematosus (SLE) as a possible indicator of renal involvement. 28 patients, representing a broad range of disease activity were admitted to our study. Urinary proteins were separated on 4-20% gradient SDS-PAGE gels, under reducing conditions, and transblotted to nitrocellulose. Western blots were developed with a polyvalent goat-anti-human C3d antiserum, and an alkaline phosphatase-conjugated rabbit anti-goat IgG. Three patterns were obtained: 1) no bands detected; 2) bands suggesting the presence of intact C3; and 3) samples with additional low molecular (< 4 x 10(4)) bands. The 12 patients with no C3 bands had minimal disease activity (e.g. fatigue, arthralgia, arthritis, rash, oral ulcers). The seven patients with intact C3 patterns also had minimally active disease. Their primary clinical findings included fatigue, pleurisy, renal disease which had been treated, hemolytic anemia, and arthritis. Patients with low molecular weight C3 fragments in their urine formed two sub-sets, based upon their presenting features. The first group had severe disease and contained all patients with active lupus nephritis (n = 4), while the second consisted of non-renal patients with primary clinical findings of moderate disease activity (e.g. thrombocytopenia, pneumonitis, arthritis). Our results suggest urinary excretion of low molecular weight C3 fragments correlates with active renal disease, but is a variable finding among SLE patients with non-renal manifestations of disease activity.","PeriodicalId":77007,"journal":{"name":"Applied and theoretical electrophoresis : the official journal of the International Electrophoresis Society","volume":"3 6","pages":"265-9"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19188910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Wave packets analysis of two-dimensional protein maps: a new approach to study the diversity of immunoglobulins. 二维蛋白图谱的波包分析:研究免疫球蛋白多样性的新方法。

Applied and theoretical electrophoresis : the official journal of the International Electrophoresis Society Pub Date : 1993-01-01

A Zahnd, J D Tissot, D F Hochstrasser

引用次数: 0

Capillary electrophoresis to measure sulfoesterase activity on chondroitin sulfate and heparin derived disaccharides. 毛细管电泳测定硫酸软骨素和肝素衍生双糖的硫酯酶活性。

Applied and theoretical electrophoresis : the official journal of the International Electrophoresis Society Pub Date : 1993-01-01

A Pervin, K Gu, R J Linhardt

引用次数: 0

Exploratory data analysis in two-dimensional electrophoresis. 双向电泳的探索性数据分析。

Applied and theoretical electrophoresis : the official journal of the International Electrophoresis Society Pub Date : 1993-01-01

S P Caudill, J E Myrick, M K Robinson

{"title":"Exploratory data analysis in two-dimensional electrophoresis.","authors":"S P Caudill, J E Myrick, M K Robinson","doi":"","DOIUrl":"","url":null,"abstract":"The use of computerized matching of proteins in the analysis of multiple two-dimensional electrophoresis (2DE) gels creates volumes of data that are readily accessible for exploratory analysis. When these data are used in health-effects studies or in studies to identify factors associated with particular diseases, hundreds or even thousands of hypotheses can be tested. Interpreting so many hypothesis tests requires some preliminary statistical evaluations of the data. In addition, prior to the preliminary statistical evaluations and subsequent hypothesis tests, accurate protein quantification and correct protein matching must be verified. In this report we present an approach used at the Centers for Disease Control to address these issues. This approach consists of a randomized experimental design incorporating replicate gels for each specimen, gel image analysis, protein matching, editing, Boolean unions of all gels to obtain correspondences and contradictions of match identification numbers, resolution of correspondences and contradictions, statistical tests to identify outliers, and finally an assessment of statistical and practical significance to focus attention on the proteins most likely to be associated with the effects under study. We illustrate our approach with data from an exploratory exposure-response study.","PeriodicalId":77007,"journal":{"name":"Applied and theoretical electrophoresis : the official journal of the International Electrophoresis Society","volume":"3 3-4","pages":"133-6"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19494976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Use of resonance ionization spectroscopy to detect DNA bands on ultrathin spin-coated gels. 利用共振电离光谱检测超薄自旋涂覆凝胶上的DNA带。

Applied and theoretical electrophoresis : the official journal of the International Electrophoresis Society Pub Date : 1993-01-01

M J Doktycz, W A Gibson, H F Arlinghaus, R C Allen, K B Jacobson

引用次数: 0

Non-isotopic detection of single-stranded conformation polymorphisms using ethidium bromide/UV light. 使用溴化乙啶/紫外光进行单链构象多态性的非同位素检测。

Applied and theoretical electrophoresis : the official journal of the International Electrophoresis Society Pub Date : 1993-01-01

W G Ballhausen, C Kraus

引用次数: 0

A new method of scaling up free flow electrophoresis. 一种放大自由流动电泳的新方法。

Applied and theoretical electrophoresis : the official journal of the International Electrophoresis Society Pub Date : 1993-01-01

P Painuly, M C Roman

引用次数: 0

Enhanced detection of PCR products through use of TOTO and YOYO intercalating dyes with laser induced fluorescence--capillary electrophoresis. 利用TOTO和YOYO插层染料激光诱导荧光-毛细管电泳增强PCR产物的检测。

Applied and theoretical electrophoresis : the official journal of the International Electrophoresis Society Pub Date : 1993-01-01

K Srinivasan, S C Morris, J E Girard, M C Kline, D J Reeder

引用次数: 0

Strategies for pH gradients' optimization. pH梯度优化策略。

Applied and theoretical electrophoresis : the official journal of the International Electrophoresis Society Pub Date : 1993-01-01

F C Celentano, E Gianazza

引用次数: 0

Splitting merged spots in two-dimensional polyacrylamide gel electrophoresis gel images. 分割合并点在二维聚丙烯酰胺凝胶电泳凝胶图像。

Applied and theoretical electrophoresis : the official journal of the International Electrophoresis Society Pub Date : 1993-01-01

P F Lemkin, J E Myrick, K M Upton

{"title":"Splitting merged spots in two-dimensional polyacrylamide gel electrophoresis gel images.","authors":"P F Lemkin, J E Myrick, K M Upton","doi":"","DOIUrl":"","url":null,"abstract":"We describe a heuristic computer algorithm using boundary analysis for improving spot finding and spot quantitation of large saturated or near-saturated spots in two-dimensional polyacrylamide electrophoresis gels. This spot quantitation is done using spot segmentation, which consists of spot finding and subsequent quantification steps. Occasionally, clusters of large saturated spots may become merged during spot finding. To correct this, the merged spots must be cut apart before quantitation. It is generally obvious from viewing the merged spot's border where they should be cut--at opposing saddlepoints (concavities in the boundary). The algorithm uses an analysis of the missegmented spot's boundary when a saturated spot is detected. If a near-saturated spot is larger than a given size, the spot segmenter program attempts to merge saturated fragments. When merging occurs, the segmenter program analyses the boundary to see if the spot should be split. The new algorithm first finds all robust concavities and then tries to match complementary ones. These paired concavities are then used to guide cutting of the missegmented spot into two or more separate spot regions. Finally, control is returned to the segmenter program to reprocess the data as a set of smaller separated spots.","PeriodicalId":77007,"journal":{"name":"Applied and theoretical electrophoresis : the official journal of the International Electrophoresis Society","volume":"3 3-4","pages":"163-72"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19494980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0