A new method of scaling up free flow electrophoresis.

Abstract

Free flow electrophoresis (FFE) has been utilized for the separation of proteins and cells for many years and has evolved into the most promising method of continuous separation of biomolecules. One of the major drawbacks inherent in FFE in the past, however, is the thermal convection caused by joule heating which occurs whenever a current is applied across a conducting liquid medium. To provide efficient heat dissipation, the cross-section of traditional FFE units is restricted to approximately 1 mm, which limits sample throughput. A new continuous FFE apparatus, which internally cools the separation unit by passing water through aligned capillary tubes, has been developed. This innovation allows scale-up of the separation without thermal convection. Results of separations of dyes and proteins are presented.