Acta paediatrica Japonica : Overseas edition最新文献_第7页

Successful polymerase chain reaction-based diagnosis of fungal meningitis in a patient with chronic granulomatous disease. 基于聚合酶链反应的慢性肉芽肿病真菌性脑膜炎成功诊断

Acta paediatrica Japonica : Overseas edition Pub Date : 1998-08-01 DOI: 10.1111/j.1442-200x.1998.tb01947.x

I Tsuge, K Makimura, J Natsume, T Kubota, S Hasegawa, T Kawabe, S Nakashima, K Aso, T Negoro, K Watanabe

引用次数: 6

Perinatal management of congenital complete atrioventricular block: report of nine cases. 先天性完全性房室传导阻滞的围产期处理:附9例报告。

Acta paediatrica Japonica : Overseas edition Pub Date : 1998-08-01 DOI: 10.1111/j.1442-200x.1998.tb01943.x

J Fukushige, N Takahashi, H Igarashi, H Nakayama, K Inoue, T Hijii

{"title":"Perinatal management of congenital complete atrioventricular block: report of nine cases.","authors":"J Fukushige, N Takahashi, H Igarashi, H Nakayama, K Inoue, T Hijii","doi":"10.1111/j.1442-200x.1998.tb01943.x","DOIUrl":"https://doi.org/10.1111/j.1442-200x.1998.tb01943.x","url":null,"abstract":"Background: The addition of fetal ultrasonography has allowed the prenatal diagnosis and observation of congenital complete atrioventricular block (CCAVB). Thus, the management of the affected fetuses should be modified accordingly.Methods: The medical records were reviewed to identify patients with CCAVB, and clinical and laboratory data were collected.Results: Nine patients with CCAVB, diagnosed prenatally, were identified. The gestational age at the time of diagnosis ranged from 18 to 38 weeks (median, 25 weeks). The gestational age and birthweight ranged from 29 to 41 weeks and from 1420 to 4075 g, respectively. An accompanying congenital cardiac anomaly was noted in three cases, including one with polysplenia syndrome. The heart rate at birth ranged from 30 to 80 bpm. In three neonates, isoproterenol was given for bradycardia. One fetus with hydrops fetalis associated with complex heart disease was treated with maternally administered digoxin, with resolution of the fluid accumulation. A permanent pacemaker was implanted in six cases: two within 1 day of birth; two during the neonatal period; one at 51 days; and the other at 2 years and 11 months of age. There were no deaths or major complications and all have remained well beyond their infancy.Conclusions: With the improvement of diagnostic methodology, more cases with CCAVB, including those with complex heart disease, could be diagnosed during fetal life. Their outcome has been steadily improving with the advances in perinatal management, pacemakers and implant techniques, irrespective of associated structural cardiac defects.","PeriodicalId":76975,"journal":{"name":"Acta paediatrica Japonica : Overseas edition","volume":"40 4","pages":"337-40"},"PeriodicalIF":0.0,"publicationDate":"1998-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1442-200x.1998.tb01943.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20659681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Modification of the MTT method for the study of bilirubin cytotoxicity. MTT法研究胆红素细胞毒性的改进。

Acta paediatrica Japonica : Overseas edition Pub Date : 1998-08-01 DOI: 10.1111/j.1442-200x.1998.tb01938.x

K C Ngai, C Y Yeung, J Karlberg

{"title":"Modification of the MTT method for the study of bilirubin cytotoxicity.","authors":"K C Ngai, C Y Yeung, J Karlberg","doi":"10.1111/j.1442-200x.1998.tb01938.x","DOIUrl":"https://doi.org/10.1111/j.1442-200x.1998.tb01938.x","url":null,"abstract":"Background: We propose a modification of the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] method to study the cytotoxicity of bilirubin. The original method involves reading the intensity of a purplish blue color resulting from the conversion of MTT to formazan crystals by the mitochondria of viable cells. We have found that when the method is applied to study the effect of bilirubin on growing cells, precipitation of the yellow bilirubin pigment interferes with the colorimetric reading.Methods: A human liver cell line was used. The interference of bilirubin deposition on the MTT assay was investigated by comparing the value of optical density of the MTT solution in the presence and absence of bilirubin. The effect of 0.04 mol/L HCL-isopropanol on the bilirubin precipitate was tested by recovering the amount of bilirubin from the wells after the isopropanol treatment.Results: Bilirubin deposition increases MTT reading by 10-24%. Hydrochloride-isopropanol (0.04 mol/L) dissolves MTT formazan only without disturbing the bilirubin precipitates. The bilirubin extracted into the supernatant was less than 5% of the total bilirubin deposited.Discussion: This indirect MTT assay, as developed in this study, could eliminate the interference of bilirubin deposits and serve as a good method for the study of bilirubin cytotoxicity.","PeriodicalId":76975,"journal":{"name":"Acta paediatrica Japonica : Overseas edition","volume":"40 4","pages":"313-7"},"PeriodicalIF":0.0,"publicationDate":"1998-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1442-200x.1998.tb01938.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20659676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16

Recurrent hepatosplenomegaly and peripheral blood cytopenia, persistent Epstein-Barr virus infection and central nervous system manifestation in a patient with lymphadenopathy and low serum uric acid. 1例淋巴结病伴低血清尿酸患者复发性肝脾肿大、外周血细胞减少、持续性eb病毒感染及中枢神经系统表现

Acta paediatrica Japonica : Overseas edition Pub Date : 1998-08-01 DOI: 10.1111/j.1442-200x.1998.tb01949.x

K Sugita, S Hagisawa, Y Satoh, M Eguchi, T Furukawa

{"title":"Recurrent hepatosplenomegaly and peripheral blood cytopenia, persistent Epstein-Barr virus infection and central nervous system manifestation in a patient with lymphadenopathy and low serum uric acid.","authors":"K Sugita, S Hagisawa, Y Satoh, M Eguchi, T Furukawa","doi":"10.1111/j.1442-200x.1998.tb01949.x","DOIUrl":"https://doi.org/10.1111/j.1442-200x.1998.tb01949.x","url":null,"abstract":"We describe a 27-month-old boy who was first admitted to our hospital on 7 January 1995 with nasal bleeding. From 6 months of age he has had lymphadenopathy, low levels of serum uric acid, increased levels of serum lactate dehydrogenase and hyper gamma-globulinemia. From the age of 18 months he has had persistent Epstein-Barr virus (EBV) infection (target cells; B cells), recurrent episodes of thrombocytopenia, anemia and hepatosplenomegaly. Dysmobility of the left leg and arm from a central nervous system complication during a relapse with pancytopenia on March 1995, was also observed. Relapses of thrombocytopenia with increases of platelet-associated immunoglobulin G and hepatosplenomegaly have been observed approximately every 2 months, and two relapses of pancytopenia were accompanied with weak positivity of Coombs test and low level of haptoglobin. These recurrent episodes were improved with prednisolone. However, now in June 1997 we have not been able to diagnose what underlies the above clinical symptoms, except that the patient has a persistent EBV infection.","PeriodicalId":76975,"journal":{"name":"Acta paediatrica Japonica : Overseas edition","volume":"40 4","pages":"362-6"},"PeriodicalIF":0.0,"publicationDate":"1998-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1442-200x.1998.tb01949.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20659636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

An autopsy case of fetal Gaucher disease. 胎儿戈谢病尸检一例。

Acta paediatrica Japonica : Overseas edition Pub Date : 1998-08-01 DOI: 10.1111/j.1442-200x.1998.tb01952.x

Y Adachi, Y Kobayashi, H Ida, R Yasumizu, A Okamura, H Kayatani, N Teranishi, M Inaba, A Sugihara, H Genba, Y Eto, S Ikehara

引用次数: 7

Assessment of D-glucose transport kinetics in the perfused human placenta: an in vitro study. d -葡萄糖在灌注人胎盘中的转运动力学评估:一项体外研究。

Acta paediatrica Japonica : Overseas edition Pub Date : 1998-08-01 DOI: 10.1111/j.1442-200x.1998.tb01937.x

M Nandakumaran, B R Dev, M Makhseed, T N Sugathan

{"title":"Assessment of D-glucose transport kinetics in the perfused human placenta: an in vitro study.","authors":"M Nandakumaran, B R Dev, M Makhseed, T N Sugathan","doi":"10.1111/j.1442-200x.1998.tb01937.x","DOIUrl":"https://doi.org/10.1111/j.1442-200x.1998.tb01937.x","url":null,"abstract":"Background: There have been no reports to date on glucose transport kinetics and the effect of graded hyperglycemia in the human placenta in non-steady-state conditions.Methods: The transport kinetics of D-glucose in the human placenta was investigated in non-steady state conditions in vitro using perfusion of isolated placental lobules. National Cancer Tissue Culture (NCTC) 135 culture medium, diluted with Earle's buffered salt solution was used as the perfusate. 14C-Labeled D-glucose and water as reference were injected as a single bolus into the maternal arterial perfusate. Perfusate samples were collected and analyzed from the maternal and fetal venous outflows.Results: In four successful perfusions, differential transport rates of glucose in the maternal-fetal direction averaged 1.03, 1.02, 1.09, 1.04 and 1.03 times those of corresponding tritiated water transport rates for 10, 25, 50, 75 and 90% of efflux fractions, respectively. Glucose transport fraction, expressed as percentage of injected maternal dose averaged 84 +/- 3.1% of water transport fraction in the four perfusions. Glucose kinetic parameters expressed as area under the curve, elimination constant (Kel), clearance, time for maximum response, absorption rate and elimination rate averaged 0.25, 0.29, 3.96, 1.02, 0.25 and 0.18 times that of the corresponding tritiated water value, respectively. Neither the different kinetic parameters nor the transport fraction indices differed significantly when glucose concentrations in the same perfusions were raised successively from 5.56 to 27.80 and then to 55.6 mmol/L.Conclusions: We speculate that within physiological limits, hyperglycemia per se plays no significant part in altering glucose transport kinetics across the human placenta in the maternal-fetal direction.","PeriodicalId":76975,"journal":{"name":"Acta paediatrica Japonica : Overseas edition","volume":"40 4","pages":"307-12"},"PeriodicalIF":0.0,"publicationDate":"1998-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1442-200x.1998.tb01937.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20659815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Prevalence of GBV-C/HGV infection in pregnant Japanese women. 日本孕妇GBV-C/HGV感染的流行情况。

Acta paediatrica Japonica : Overseas edition Pub Date : 1998-08-01 DOI: 10.1111/j.1442-200x.1998.tb01955.x

K Sugiyama, K Goto, Y Miyake, F Mizutani, K Terabe, R Li, Y Wada, S Kajiura

引用次数: 1

The effect of live measles vaccines on serum vitamin A levels in healthy children. 麻疹活疫苗对健康儿童血清维生素A水平的影响

Acta paediatrica Japonica : Overseas edition Pub Date : 1998-08-01 DOI: 10.1111/j.1442-200x.1998.tb01945.x

S S Yalçin, K Yurdakök, I Ozalp, T Coşkun

{"title":"The effect of live measles vaccines on serum vitamin A levels in healthy children.","authors":"S S Yalçin, K Yurdakök, I Ozalp, T Coşkun","doi":"10.1111/j.1442-200x.1998.tb01945.x","DOIUrl":"https://doi.org/10.1111/j.1442-200x.1998.tb01945.x","url":null,"abstract":"Objective: Serum retinol levels have been shown to be depressed during measles infection. This study aims to demonstrate whether there is any decrease in serum vitamin A level following immunization with live viral vaccine and its relation with vaccine seroconversion in children with measles. Since many children receive measles vaccine alone or in combination with measles-mumps-rubella vaccine, we studied serum vitamin A levels and antibody levels in healthy, well-nourished children before and after immunization with monovalent and combined live attenuated measles vaccine.Methods: The first group included 21 healthy children between the ages of 9-11 months who received live measles (Schwarz) vaccine. There were also 21 healthy children (range 14-20 months of age) who received measles-mumps-rubella Trimovax (Pasteur Merieux) vaccine. All children were tested for serum vitamin A levels before vaccination, on days 9-14 and 30-42 following both vaccinations. Measles specific antibody levels were also measured on admission and 30-42 days following vaccinations.Results: In both vaccination groups, mean serum vitamin A levels reduced significantly on days 9-14, but increased slightly on days 30-42 in the measles-mumps-rubella vaccinated group (P < 0.05). The baseline and follow-up levels of mean serum vitamin A did not differ between seroconverted and nonseroconverted cases within the measles vaccinated group.Conclusion: Serum vitamin A levels are reduced following vaccination with monovalent and combined live attenuated measles vaccines.","PeriodicalId":76975,"journal":{"name":"Acta paediatrica Japonica : Overseas edition","volume":"40 4","pages":"345-9"},"PeriodicalIF":0.0,"publicationDate":"1998-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1442-200x.1998.tb01945.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20659683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Mutation in the gene encoding the fibroblast growth factor receptor-3 in Korean children with achondroplasia. 韩国软骨发育不全儿童成纤维细胞生长因子受体-3基因编码突变。

Acta paediatrica Japonica : Overseas edition Pub Date : 1998-08-01 DOI: 10.1111/j.1442-200x.1998.tb01940.x

S W Yang, H Kitoh, Y Yamada, H Goto, N Ogasawara

引用次数: 1

Bacterial croup caused by Pasteurella haemolytica. 溶血巴斯德氏杆菌引起的细菌群。

Acta paediatrica Japonica : Overseas edition Pub Date : 1998-08-01 DOI: 10.1111/j.1442-200x.1998.tb01948.x

T Watanabe, M Sato, T Abe, Y Oda

引用次数: 10