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Zeitschrift fur medizinische Laboratoriumsdiagnostik最新文献

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[Lipoprotein (a)--is it a connecting link between fat metabolism and coagulation?]. [脂蛋白(a)——它是脂肪代谢和凝血之间的连接纽带吗?]
Zeitschrift fur medizinische Laboratoriumsdiagnostik Pub Date : 1991-01-01
W F Riesen
{"title":"[Lipoprotein (a)--is it a connecting link between fat metabolism and coagulation?].","authors":"W F Riesen","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":76852,"journal":{"name":"Zeitschrift fur medizinische Laboratoriumsdiagnostik","volume":"32 3-4","pages":"147-9"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12993520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Measruement of capillary blood using the Coulter S Plus VI, the Coulter T660 and the Coulter T890]. [使用Coulter S Plus VI、Coulter T660和Coulter T890测量毛细血管血液]。
Zeitschrift fur medizinische Laboratoriumsdiagnostik Pub Date : 1991-01-01
C Ruby
{"title":"[Measruement of capillary blood using the Coulter S Plus VI, the Coulter T660 and the Coulter T890].","authors":"C Ruby","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":76852,"journal":{"name":"Zeitschrift fur medizinische Laboratoriumsdiagnostik","volume":"32 3-4","pages":"217-9"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13057581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Visual test evaluation of the results for the semiquantitative determination of cystine in urine using the nickel/dithionite (Ni2+/S2O42-) test]. [用镍/二亚铁酸盐(Ni2+/S2O42-)试验半定量测定尿中胱氨酸的目测评价]。
Zeitschrift fur medizinische Laboratoriumsdiagnostik Pub Date : 1991-01-01
O Kilian, W Berg
{"title":"[Visual test evaluation of the results for the semiquantitative determination of cystine in urine using the nickel/dithionite (Ni2+/S2O42-) test].","authors":"O Kilian,&nbsp;W Berg","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The Ni2+/S2O4(2-)-test makes semiquantitative assessment of cystine concentration in urine possible. The reaction principle of the method is based on the formation of a nickel/cysteine/sulfide complex of brown colour. Visual test evaluation is done by classifying the colour intensity of the sample/reagent mixture according to an exact colour shade. Each colour shade on a colour reference table is correlated to a certain range of cystine concentration. Cystine determination is described in detail for two ranges (0.60 and 1.25 mmol/l).</p>","PeriodicalId":76852,"journal":{"name":"Zeitschrift fur medizinische Laboratoriumsdiagnostik","volume":"32 1","pages":"50-6"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13224456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Protein adsorption at the tips from synthetic polymers as the cause of incorrect titer analysis of interferons in antiviral bioassays]. [抗病毒生物测定中干扰素滴度分析不正确的原因是合成聚合物尖端处的蛋白质吸附]。
Zeitschrift fur medizinische Laboratoriumsdiagnostik Pub Date : 1991-01-01
M Tonew, E Ohme, D Gerlach, R H Wondraczek
{"title":"[Protein adsorption at the tips from synthetic polymers as the cause of incorrect titer analysis of interferons in antiviral bioassays].","authors":"M Tonew,&nbsp;E Ohme,&nbsp;D Gerlach,&nbsp;R H Wondraczek","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The determination of the biological activity of interferons are carried out on the basis of the inhibition of the cytopathic effect of vesicular stomatitis virus on MDBK cells. Using microtiter-plates and tips made of synthetic polymers incorrect data may be observed due to the adsorption of the protein interferon to the tips and, thus, carryover of activity. The following recommendations may help to avoid this kind of mistakes: 1. Generally changing of the tips, at least for the highest concentrations. 2. Reduction of high initial activities by an appropriate pre-dilution. 3. Addition of the detergent Triton X 100 in concentrations between 50 to 100 mg/l to the dilution media for interferon.</p>","PeriodicalId":76852,"journal":{"name":"Zeitschrift fur medizinische Laboratoriumsdiagnostik","volume":"32 2","pages":"108-13"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12814644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Evaluation of methods for the analysis of low insulin concentrations in serum]. [血清低胰岛素浓度分析方法评价]。
Zeitschrift fur medizinische Laboratoriumsdiagnostik Pub Date : 1991-01-01
K Jung, S Klotzek, B D Schulze
{"title":"[Evaluation of methods for the analysis of low insulin concentrations in serum].","authors":"K Jung,&nbsp;S Klotzek,&nbsp;B D Schulze","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Three methods of the determination of insulin in serum were optimized in order to determine low concentrations of this analyte reliably (Method I: enzymatic after acidic hydrolysis of inulin; Method II: by anthrone/sulfuric acid reagent; Method III: by cysteine/tryptophan reagent). The interfering reaction of glucose in the methods I and II was avoided by the preincubation of samples with glucose oxidase/catalase reagent. The detection limit of all three methods was below 5 mg/l and the within-run precision amounted to values below 5%. Method I and II gave identical results. In comparison with these two methods, method III resulted in inulin values by about 8% lower, because inulin, depending on the lot used, contained alkali-labile fractions which were destroyed during the sample preparation. Method II is recommended for routine measurements because of its an analytical reliability and its cost-effectiveness.</p>","PeriodicalId":76852,"journal":{"name":"Zeitschrift fur medizinische Laboratoriumsdiagnostik","volume":"32 1","pages":"23-9"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13224453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Pathobiogenetic concept of Duchenne muscular dystrophy following the discovery of dystrophin as the deficient protein]. 【发现肌营养不良蛋白为缺陷蛋白后，杜氏肌营养不良的发病机制概念】。
Zeitschrift fur medizinische Laboratoriumsdiagnostik Pub Date : 1991-01-01
D Kunze, B Rüstow
{"title":"[Pathobiogenetic concept of Duchenne muscular dystrophy following the discovery of dystrophin as the deficient protein].","authors":"D Kunze,&nbsp;B Rüstow","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":76852,"journal":{"name":"Zeitschrift fur medizinische Laboratoriumsdiagnostik","volume":"32 3-4","pages":"141-6"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13056872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Agent-conditioned influence on carbohydrate metabolism and energy state in liver and lung of rats in endotoxic shock]. [内毒素休克大鼠肝、肺碳水化合物代谢及能量状态的影响]。
Zeitschrift fur medizinische Laboratoriumsdiagnostik Pub Date : 1991-01-01
S Kopprasch, K Kociok, M Kohl, D W Scheuch
{"title":"[Agent-conditioned influence on carbohydrate metabolism and energy state in liver and lung of rats in endotoxic shock].","authors":"S Kopprasch,&nbsp;K Kociok,&nbsp;M Kohl,&nbsp;D W Scheuch","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The pretreatment of rats with the substance LPH and LXU caused decrease of lethality in endotoxin shock and suppression of the following changes 6 hours post endotoxin: Increase of plasma ICDH activity, decrease in platelet count, decrease of the hepatic energy state and increase of the ATP content in the lung. The catabolic glucose metabolism during endotoxin shock was not influenced by LPH and LXU administration.</p>","PeriodicalId":76852,"journal":{"name":"Zeitschrift fur medizinische Laboratoriumsdiagnostik","volume":"32 3-4","pages":"167-72"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13056876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Application of the monodimensional electrophoresis for the separation of glycosaminoglycans on Cellogel cellulose acetate strip. 一维电泳在醋酸纤维素条上分离糖胺聚糖的应用。
Zeitschrift fur medizinische Laboratoriumsdiagnostik Pub Date : 1991-01-01
D Juretić, J Bingulac-Popović, K Lipovac, I Ligutić
{"title":"Application of the monodimensional electrophoresis for the separation of glycosaminoglycans on Cellogel cellulose acetate strip.","authors":"D Juretić,&nbsp;J Bingulac-Popović,&nbsp;K Lipovac,&nbsp;I Ligutić","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A monodimensional electrophoretic method for the separation of glycosaminoglycans on Titan III Zip Zone cellulose acetate plate based on their different electrophoretic mobilities in barium acetate and different solubilities in ethanol was applied to the Chemetron electrophoretic equipment. Improved timing of individual steps of electrophoretic run, additional cooling and pressure must be introduced for optimal separation of glycosaminoglycans mixture (dermatan sulphate, heparan sulphate, hyaluronic acid, chondroitin-4-sulphate, chondroitin-6-sulphate and keratan sulphate) resulting in five well separated sharp bands. By all these changes in the original procedure of Hopwood and Harrison, the separation of chondroitin-4-sulphate and chondroitin-6-sulphate was not achieved. The modified procedure on Cellogel strip is suitable for the screening of mucopolysaccharidoses.</p>","PeriodicalId":76852,"journal":{"name":"Zeitschrift fur medizinische Laboratoriumsdiagnostik","volume":"32 3-4","pages":"180-7"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13056878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[An average variation for glucose determination using the ECA 20]. [使用ECA测定葡萄糖的平均变化]。
Zeitschrift fur medizinische Laboratoriumsdiagnostik Pub Date : 1991-01-01
I Gödicke, B Brys, W Gödicke
{"title":"[An average variation for glucose determination using the ECA 20].","authors":"I Gödicke,&nbsp;B Brys,&nbsp;W Gödicke","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":76852,"journal":{"name":"Zeitschrift fur medizinische Laboratoriumsdiagnostik","volume":"32 3-4","pages":"213-6"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13057580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Immunoturbidimetric determination of C-3 components of complement systems using nonlinear calibration]. [免疫比浊法测定补体系统中C-3组分的非线性校准]。
Zeitschrift fur medizinische Laboratoriumsdiagnostik Pub Date : 1991-01-01
J Rohácek, V Semrád, E Klierová, M Zápotocná
{"title":"[Immunoturbidimetric determination of C-3 components of complement systems using nonlinear calibration].","authors":"J Rohácek,&nbsp;V Semrád,&nbsp;E Klierová,&nbsp;M Zápotocná","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A method for the immunoturbidimetric analysis of the C-3-component in the complement system was elaborated by means of the antiserum Q-SwAHu/C3 USOL (SEVAG) Praha. A diluted human control serum USOL (SEVAG) Praha with declared values of plasma proteins was applied as a standard solution. The relation between concentration and absorption in an eight step calibration series is well described by a parabola of the 2nd degree. The precision in series and the accuracy of the method are mentioned. The proposed technique is in a relatively good correlation with the radial immunodiffusion according to MANCINI.</p>","PeriodicalId":76852,"journal":{"name":"Zeitschrift fur medizinische Laboratoriumsdiagnostik","volume":"32 1","pages":"15-8"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13066897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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