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[The direct detection of volatile fatty acids by gas chromatography in microbiological diagnosis]. 气相色谱法直接检测挥发性脂肪酸在微生物诊断中的应用
Zeitschrift fur medizinische Laboratoriumsdiagnostik Pub Date : 1990-01-01
S Socolowsky, C Höhne, D Sandow
{"title":"[The direct detection of volatile fatty acids by gas chromatography in microbiological diagnosis].","authors":"S Socolowsky,&nbsp;C Höhne,&nbsp;D Sandow","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The gas chromatographic analysis of volatile fatty acids (VFA) is of great significance in the diagnosis of anaerobes and anaerobic infections, respectively. The ether extraction commonly used for this purpose is relatively consuming in time and material. In this relation, the presented method of detection of VFA in the aqueous phase directly shows essential simplifications but has a lower sensitivity. Therefore, this method cannot replace the ether extraction to investigate broth cultures of anaerobes for taxonomical purposes. But it enables the determination of VFA with sufficient sensitivity in clinical materials of patients. Herewith, the detection of acetic acid only indicates an infection without anaerobes if the concentration is higher than 20 mg/100 ml. On the contrary, a high correlation exists between the detection of n-butyric acid as well as isovaleric acid and the cultural isolation of anaerobic microorganisms.</p>","PeriodicalId":76852,"journal":{"name":"Zeitschrift fur medizinische Laboratoriumsdiagnostik","volume":"31 8","pages":"445-52"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13250269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Quantitative determination of apolipoprotein AI in serum by the use of monoclonal antibodies in a noncompetitive ELISA]. [在非竞争ELISA中使用单克隆抗体定量测定血清载脂蛋白AI]。
Zeitschrift fur medizinische Laboratoriumsdiagnostik Pub Date : 1990-01-01
L Winkler, D W Müller, R Dargel, L Jäger
{"title":"[Quantitative determination of apolipoprotein AI in serum by the use of monoclonal antibodies in a noncompetitive ELISA].","authors":"L Winkler,&nbsp;D W Müller,&nbsp;R Dargel,&nbsp;L Jäger","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A methodical base for estimation of apolipoprotein AI (apo AI) in the serum using a monoclonal antibody in a non-competitive ELISA is described. The technical approach is adjusted to commercial materials of the DDR industry. The assay allows to determine apo AI within the range of 5-100 micrograms apo AI/I. The Vk was 4% (within), and 8% (day to day). The monoclonal antibody used is directed against a native epitope which is fully exposed in the serum. Application of the SUMAL system further improves the practicability of the method. The assay should be suitable particularly for the recognition of epitopes of apo AI in serum HDL fractions associated with an atherogenic risk. The values obtained by the ELISA correspond to those measured turbidimetrically using polyclonal antibodies.</p>","PeriodicalId":76852,"journal":{"name":"Zeitschrift fur medizinische Laboratoriumsdiagnostik","volume":"31 3","pages":"159-64"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13270215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of kallikrein-kinin system activation by factor XII f pretreatment on experimental hemorrhagic shock. 因子XII预处理激活钾likrein- kinins系统对实验性失血性休克的影响。
Zeitschrift fur medizinische Laboratoriumsdiagnostik Pub Date : 1990-01-01
M Taucher, J Grässler, H Kühne, D W Scheuch
{"title":"Effect of kallikrein-kinin system activation by factor XII f pretreatment on experimental hemorrhagic shock.","authors":"M Taucher,&nbsp;J Grässler,&nbsp;H Kühne,&nbsp;D W Scheuch","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Effects of Hageman factor fragment (factor XIIf) administration on shock induction were studied in anesthetized male Wistar rats with hemorrhagic shock. Administration of factor XIIf in non-hemorrhaged rats resulted in an immediate decrease of mean arterial pressure (MAP) for 5 min and in significant increases of blood glucose, lactate and hematocrit (Hct) as well as in a strong tendency for plasma kallikreinogen (KKN) to decrease. At the beginning of bleeding (15 min after factor XIIf administration) MAP has been normalized again. During subsequent hemorrhage pretreated rats showed a sharper decrease in MAP which remained significantly lower up to 25% of estimated blood volume (EBV) and recovered more slowly to normal level after hemorrhage. Blood glucose, lactate and Hct were significantly higher in pretreated animals after hemorrhage of 30% EBV. KKN depicted significant lower values during hemorrhage and in the posthemorrhagic period. Mortality within the observation time increased from 20% (control group) to 60% (pretreated animals). The results demonstrate that prehemorrhagic kallikrein-kinin system (KKS) activation induced an increased severity of shock state with higher mortality.</p>","PeriodicalId":76852,"journal":{"name":"Zeitschrift fur medizinische Laboratoriumsdiagnostik","volume":"31 1","pages":"13-7"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13480135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Enzyme immunoassays for the quantification of human gamma-gamma-enolase (NSE)]. [定量测定人γ - γ -烯醇化酶(NSE)的酶免疫分析法]。
Zeitschrift fur medizinische Laboratoriumsdiagnostik Pub Date : 1990-01-01
J Gross, J Zinsmeyer, R Pohle, R Moller, H Schmechta, B Porstmann, T Porstmann, B Brux
{"title":"[Enzyme immunoassays for the quantification of human gamma-gamma-enolase (NSE)].","authors":"J Gross,&nbsp;J Zinsmeyer,&nbsp;R Pohle,&nbsp;R Moller,&nbsp;H Schmechta,&nbsp;B Porstmann,&nbsp;T Porstmann,&nbsp;B Brux","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A direct two-site binding assay on the basis of antibodies from sheep for the quantification of human gamma-gamma enolase is described. The antibody was produced by immunization with human NSE coupled to horse spleen ferritin. The assay shows two feature: a decreased reactivity with NSE from rat and NSE from human serum in spite of 100% recovery of purified human brain NSE. The sheep antibody seems to react with epitopes less accessible on the rat NSE and on the NSE of human serum. The assay is characterized by gamma-gamma enolase specificity, a high sensitivity (2 pg) and a precision of CV = 3-7%.</p>","PeriodicalId":76852,"journal":{"name":"Zeitschrift fur medizinische Laboratoriumsdiagnostik","volume":"31 5","pages":"258-65"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13278272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Detection of soluble fibrin monomer complexes by the netropsin precipitation test in childhood meningitis]. [netropsin沉淀法检测儿童脑膜炎可溶性纤维蛋白单体复合物]。
Zeitschrift fur medizinische Laboratoriumsdiagnostik Pub Date : 1990-01-01
G Lutze, U Mittler, M Schulze, H Urbahn
{"title":"[Detection of soluble fibrin monomer complexes by the netropsin precipitation test in childhood meningitis].","authors":"G Lutze,&nbsp;U Mittler,&nbsp;M Schulze,&nbsp;H Urbahn","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>During a period of two years children with abacterial meningitis as well as bacterial meningitis were examined before treatment and later during disease. The new Netropsin praecipitation test according to Funke and coworkers was used to detect soluble fibrin monomer complexes. Only in a few cases of abacterial meningitis, but in the majority of cases with bacterial meningitis, a positive result had been shown. Excessive increase had been found in Waterhouse-Friderichsen syndrome. According to our results of coagulation tests we conclude 1. the ethanol gelation test is out-of-date, 2. the heparin treatment in bacterial meningitis is further indicated.</p>","PeriodicalId":76852,"journal":{"name":"Zeitschrift fur medizinische Laboratoriumsdiagnostik","volume":"31 2","pages":"97-103"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13314697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Lipoprotein-cell interactions in relation to the risk of atherosclerosis]. [脂蛋白-细胞相互作用与动脉粥样硬化风险的关系]。
Zeitschrift fur medizinische Laboratoriumsdiagnostik Pub Date : 1990-01-01
V Richter
{"title":"[Lipoprotein-cell interactions in relation to the risk of atherosclerosis].","authors":"V Richter","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":76852,"journal":{"name":"Zeitschrift fur medizinische Laboratoriumsdiagnostik","volume":"31 5","pages":"243-9"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13399196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Characterization of enzyme immunoassays for T4 determination]. [酶免疫法测定T4的特点]。
Zeitschrift fur medizinische Laboratoriumsdiagnostik Pub Date : 1990-01-01
G Hellthaler, H Keller
{"title":"[Characterization of enzyme immunoassays for T4 determination].","authors":"G Hellthaler,&nbsp;H Keller","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>We compared an enzyme immunoassay for T4 (MTPT4) developed in Halle with the Enzymun-Test T4, Boehringer Mannheim (BMT4). Calculated coefficients of correlation can not be used for interpretation of the two methods. By construction of Bias-profiles the evaluation of concentration depended precision and accuracy is possible. Both methods can be used equally in laboratory work.</p>","PeriodicalId":76852,"journal":{"name":"Zeitschrift fur medizinische Laboratoriumsdiagnostik","volume":"31 5","pages":"266-72"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13399198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The interaction of cytostatic compounds with urinary enzymes. 1. The influence on acylase from the human kidney]. 细胞抑制化合物与尿酶的相互作用。1. 对人肾酰基化酶的影响[j]。
Zeitschrift fur medizinische Laboratoriumsdiagnostik Pub Date : 1990-01-01
H J Hütter, R Pantschewa-Haschen
{"title":"[The interaction of cytostatic compounds with urinary enzymes. 1. The influence on acylase from the human kidney].","authors":"H J Hütter,&nbsp;R Pantschewa-Haschen","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Acylase, a cytosolic carboxypeptidase of human kidneys, is inhibited by various xenobiotics as well as by the physiological pH of human urine in the range of pH 4.5-5.8. Caused by this, the enzyme is unsuited for the demonstration of nephrotoxic processes.</p>","PeriodicalId":76852,"journal":{"name":"Zeitschrift fur medizinische Laboratoriumsdiagnostik","volume":"31 4","pages":"225-30"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13530920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Multiple forms of gamma-glutamyltransferase and lipoproteins. 多种形式的谷氨酰转移酶和脂蛋白。
Zeitschrift fur medizinische Laboratoriumsdiagnostik Pub Date : 1990-01-01
B Kunovic, B Straus, I Cepelak
{"title":"Multiple forms of gamma-glutamyltransferase and lipoproteins.","authors":"B Kunovic,&nbsp;B Straus,&nbsp;I Cepelak","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The gamma-glutamyltransferase isoenzyme patterns originating from human serum and homogenates of liver, kidney, pancreas and intestine in the presence and in absence of isolated lipoproteins has been studied. On the basis of these results one can conclude that the distribution of a variety of gamma-glutamyltransferase activities obtained by the electrophoresis of blood serum is not a consequence of an existence of a large number of true isoenzymes, but of increased concentrations of lipoproteins which bind to the enzyme thus causing the appearance of gamma-glutamyltransferase in the region of the appropriate lipoproteins.</p>","PeriodicalId":76852,"journal":{"name":"Zeitschrift fur medizinische Laboratoriumsdiagnostik","volume":"31 5","pages":"284-9"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13138052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Pathobiochemistry: what can it do, what does it want to do?]. [病理生化:它能做什么，它想做什么?]
Zeitschrift fur medizinische Laboratoriumsdiagnostik Pub Date : 1990-01-01
W Dummler
{"title":"[Pathobiochemistry: what can it do, what does it want to do?].","authors":"W Dummler","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":76852,"journal":{"name":"Zeitschrift fur medizinische Laboratoriumsdiagnostik","volume":"31 8","pages":"403-7"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13250261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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