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Zeitschrift fur medizinische Laboratoriumsdiagnostik最新文献

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[A simple method for measuring two function parameters of phagocytizing cells using immunobeads]. [一种利用免疫珠测定吞噬细胞两个功能参数的简单方法]。
Zeitschrift fur medizinische Laboratoriumsdiagnostik Pub Date : 1990-01-01
H D Jacobi, W Sokolowska-Köhler, C Zietlow
{"title":"[A simple method for measuring two function parameters of phagocytizing cells using immunobeads].","authors":"H D Jacobi, W Sokolowska-Köhler, C Zietlow","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":76852,"journal":{"name":"Zeitschrift fur medizinische Laboratoriumsdiagnostik","volume":"31 6","pages":"351-2"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13431843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Enzymatic determination of glandular kallikrein in human pancreatic secretions]. [人胰腺分泌物中腺体钾激肽的酶法测定]。
Zeitschrift fur medizinische Laboratoriumsdiagnostik Pub Date : 1990-01-01
H Weber, W Dummler, S Liebe
{"title":"[Enzymatic determination of glandular kallikrein in human pancreatic secretions].","authors":"H Weber,&nbsp;W Dummler,&nbsp;S Liebe","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A method for the enzymatic determination of tissue kallikrein (EC 3.4. 21.8) in human pancreatic juice using D-Val-cyclohexyl-Ala-Arg-4-nitroaniline as substrate as well as the reaction conditions optimized for 37 degrees C are described. The reaction is characterized by the following kinetic parameters, determined according to Hanes and Eisenthal, Cornish-Bowden: KM = 2.10(-5) mol/l; Vmax = 0.800 mumol/(l.s). The coefficient of intra-assay variation (N = 10) was determined to 2.54%. The detection limit of the assay was found to be 8.63 nmol/(l.s).</p>","PeriodicalId":76852,"journal":{"name":"Zeitschrift fur medizinische Laboratoriumsdiagnostik","volume":"31 1","pages":"19-25"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13480136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Proteolytic activities of colonic mucosa]. [结肠黏膜的蛋白水解活性]。
Zeitschrift fur medizinische Laboratoriumsdiagnostik Pub Date : 1990-01-01
H J Hütter, V V Egorova, A A Nikitina, V A Zvetkova, A M Ugolev
{"title":"[Proteolytic activities of colonic mucosa].","authors":"H J Hütter,&nbsp;V V Egorova,&nbsp;A A Nikitina,&nbsp;V A Zvetkova,&nbsp;A M Ugolev","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The large intestine of human and dog contains considerable activities of various aminopeptidases and genuine dipeptidases. Furthermore, a dipeptidase is described, which is independant in respect to the C-terminal configuration of the peptide. This dipeptidase is an intrinsic membrane protein. The physiological significance of these enzymes is discussed.</p>","PeriodicalId":76852,"journal":{"name":"Zeitschrift fur medizinische Laboratoriumsdiagnostik","volume":"31 1","pages":"27-31"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13480137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The validity of sodium excretion in urine in normotensives and hypertensives in a selected population]. [特定人群中正常血压和高血压患者尿液中钠排泄的有效性]。
Zeitschrift fur medizinische Laboratoriumsdiagnostik Pub Date : 1990-01-01
P Köhler, M Riemekasten, A Knobloch
{"title":"[The validity of sodium excretion in urine in normotensives and hypertensives in a selected population].","authors":"P Köhler,&nbsp;M Riemekasten,&nbsp;A Knobloch","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Salt intake of 503 people from the south western area of the GDR was investigated. Salt intake is high (151 mmol/d), but lower then expected. There are no important differences between people without (140 mmol/d) and with (161 mmol/d) a essential hypertension. Salt intake of male is higher than female persons. There is no correlation between the height of salt intake and the elevation of systolic or diastolic blood pressure.</p>","PeriodicalId":76852,"journal":{"name":"Zeitschrift fur medizinische Laboratoriumsdiagnostik","volume":"31 5","pages":"290-3"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13399199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Prostacyclin and thromboxane synthesis in liver tissue in chronic liver diseases]. 慢性肝病肝组织中前列环素和血栓素的合成
Zeitschrift fur medizinische Laboratoriumsdiagnostik Pub Date : 1990-01-01
B Zipprich, C Gerhardt, C Giessler, C Taube, F W Rath, H J Mest, R Nilius
{"title":"[Prostacyclin and thromboxane synthesis in liver tissue in chronic liver diseases].","authors":"B Zipprich,&nbsp;C Gerhardt,&nbsp;C Giessler,&nbsp;C Taube,&nbsp;F W Rath,&nbsp;H J Mest,&nbsp;R Nilius","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>This paper reports on investigations of the formation of PGI2 and TXA2 using their stabile products 6-keto-PGF1 alpha and TXB2 (RIA) in liver biopsy specimens of 46 patients suffering from fatty liver (n = 19), chronic hepatitis B (n = 11), liver cirrhosis (n = 13), and miscellaneous diseases (n = 3). The measured formation rates in chronic liver disease were evaluated in comparison to a reference group (n = 19) consisting of minimal liver lesions. The 6-keto-PGF1 alpha formation correlating to the degree of the portal inflammation in the liver (morphometric evaluation). The same trend existed in relation to the intralobular inflammation. The results presented suggest in respect of analogous data in animal experiments that PGI2 is predominantly generated in mesenchymal cells of the liver and, presumably influences the course of liver diseases.</p>","PeriodicalId":76852,"journal":{"name":"Zeitschrift fur medizinische Laboratoriumsdiagnostik","volume":"31 6","pages":"299-304"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13432684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Relationship between disregulations in the hemostasis system and thrombogenesis]. 止血系统紊乱与血栓形成的关系。
Zeitschrift fur medizinische Laboratoriumsdiagnostik Pub Date : 1990-01-01
I Witt
{"title":"[Relationship between disregulations in the hemostasis system and thrombogenesis].","authors":"I Witt","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":76852,"journal":{"name":"Zeitschrift fur medizinische Laboratoriumsdiagnostik","volume":"31 1","pages":"3-11"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13336860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[A simple radioimmunologic method for the determination of unconjugated estriol (E3) in the serum of pregnant women]. [一种测定孕妇血清中非共轭雌三醇(E3)的简单放射免疫学方法]。
Zeitschrift fur medizinische Laboratoriumsdiagnostik Pub Date : 1990-01-01
W Wierz, K H Lenski
{"title":"[A simple radioimmunologic method for the determination of unconjugated estriol (E3) in the serum of pregnant women].","authors":"W Wierz,&nbsp;K H Lenski","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":76852,"journal":{"name":"Zeitschrift fur medizinische Laboratoriumsdiagnostik","volume":"31 6","pages":"347-8"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13431841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The determination of apolipoproteins Apo A-I and Apo B. A comparative study of methods]. 载脂蛋白Apo A- i和Apo b测定方法的比较研究。
Zeitschrift fur medizinische Laboratoriumsdiagnostik Pub Date : 1990-01-01
H Keller, W F Riesen
{"title":"[The determination of apolipoproteins Apo A-I and Apo B. A comparative study of methods].","authors":"H Keller,&nbsp;W F Riesen","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>We compared the analytical performance of a new in house developed ELISA and the commercial TurbiTime system for the determination of Apo AI and Apo B. Six dilutions of a poolserum, whose concentration had been determined by two other independent methods, were used as test material. A serum from which the apolipoproteins had been removed by immunadsorption was used as a diluent. Six specimens with known true values of Apo AI and Apo B were thus prepared. We established the analytical precision, the regression function and the correlation with respect to the true values and we constructed precision profiles and bias profiles. We found that the precision of turbidimetry is superior for the determination of Apo AI with concentrations less than 1 g/l. With Apo B both systems are nearly equivalent, the results of the turbidimetric methods were a little too low, the results of the ELISA a little too high. Below a concentration less than 0.5 g/l both methods are unreliable.</p>","PeriodicalId":76852,"journal":{"name":"Zeitschrift fur medizinische Laboratoriumsdiagnostik","volume":"31 4","pages":"200-10"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13271272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Lp(a)--an atherogenic risk factor in human serum? Lp(a)——人血清中的动脉粥样硬化危险因子?
Zeitschrift fur medizinische Laboratoriumsdiagnostik Pub Date : 1990-01-01
A Gries, G M Kostner
{"title":"Lp(a)--an atherogenic risk factor in human serum?","authors":"A Gries,&nbsp;G M Kostner","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":76852,"journal":{"name":"Zeitschrift fur medizinische Laboratoriumsdiagnostik","volume":"31 7","pages":"355-60"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13304967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Diagnostic importance of HDL cholesterol determination]. 【高密度脂蛋白胆固醇测定的诊断意义】。
Zeitschrift fur medizinische Laboratoriumsdiagnostik Pub Date : 1990-01-01
J Reissner, W Herrmann
{"title":"[Diagnostic importance of HDL cholesterol determination].","authors":"J Reissner,&nbsp;W Herrmann","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The present paper describes the sensitivity to quantification of changes of HDL-cholesterol in serum by two different precipitation and analytical techniques during the treatment of patients. After the precipitation of VLDL and LDL by phosphotungstic acid/magnesium chloride the chemical determination of HDL-cholesterol in serum with the Liebermann-Burchard reaction yields different results in comparison to enzymatic HDL-cholesterol determined in serum supernatant after the precipitation by polyethylene glycol 20.000. Correlation analyses of apolipoprotein A-I with enzymatic HDL-, HDL2-, HDL3-cholesterol or electrophoretic alpha-cholesterol demonstrate that the therapeutically induced changes (by training and diet) of lipid composition are more correctly reflected by the enzymatic determination of HDL-cholesterol after serum precipitation by polyethylene glycol.</p>","PeriodicalId":76852,"journal":{"name":"Zeitschrift fur medizinische Laboratoriumsdiagnostik","volume":"31 8","pages":"426-31"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13250266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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