[Protein adsorption at the tips from synthetic polymers as the cause of incorrect titer analysis of interferons in antiviral bioassays].

Abstract

The determination of the biological activity of interferons are carried out on the basis of the inhibition of the cytopathic effect of vesicular stomatitis virus on MDBK cells. Using microtiter-plates and tips made of synthetic polymers incorrect data may be observed due to the adsorption of the protein interferon to the tips and, thus, carryover of activity. The following recommendations may help to avoid this kind of mistakes: 1. Generally changing of the tips, at least for the highest concentrations. 2. Reduction of high initial activities by an appropriate pre-dilution. 3. Addition of the detergent Triton X 100 in concentrations between 50 to 100 mg/l to the dilution media for interferon.