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Zeitschrift fur medizinische Laboratoriumsdiagnostik最新文献

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[Examinations of the analytic reliability of N-acetylneuraminic acid determinations according to the pharmacopoeia of the GDR]. [根据德国药典检验n -乙酰神经氨酸测定的分析可靠性]。
Zeitschrift fur medizinische Laboratoriumsdiagnostik Pub Date : 1991-01-01
G Bollmann, B Schulz
{"title":"[Examinations of the analytic reliability of N-acetylneuraminic acid determinations according to the pharmacopoeia of the GDR].","authors":"G Bollmann,&nbsp;B Schulz","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A further investigation of the Arzneibuch (Diagnostic Laboratory Methods) GDR-method for the estimation of N-acetylneuraminic acid was performed. By this was found, that the formation of the dye complex is not still finished after 15 minutes and the reaction product shows an exponential decomposition. The decomposition rate was much greater in the reaction medium than in n-amyl alcohol. By increasing the ferric (III)-chloride concentration the specificity of the method could be essentially improved. With the fivefold ferric (III)-chloride concentration the physiological concentrations of the protein bound monosaccharides of the serum changed the accuracy of the measuring results by less than or equal to 2.0%.</p>","PeriodicalId":76852,"journal":{"name":"Zeitschrift fur medizinische Laboratoriumsdiagnostik","volume":"32 2","pages":"101-7"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13224984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Direct positive DDP 111 as a possible means of documentation for electrophoresis]. [直接阳性DDP 111作为电泳记录的可能手段]。
Zeitschrift fur medizinische Laboratoriumsdiagnostik Pub Date : 1991-01-01
G Knabe, S Gahler, K Peisker, H Roschk
{"title":"[Direct positive DDP 111 as a possible means of documentation for electrophoresis].","authors":"G Knabe,&nbsp;S Gahler,&nbsp;K Peisker,&nbsp;H Roschk","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The positivepaper DDP 111 manufactured in the Fotopapierwerk Dresden is presented. It allows to make copies (1:1) of electrophoreses. Independently of the used staining procedure it is a simple, quickly and cameraless possibly to obtain reproductions with a high quality. The DDP 111 allows to resolve the problem of documentation of electropherograms (especially of wet flat gels) and to build up collections and data files respectively.</p>","PeriodicalId":76852,"journal":{"name":"Zeitschrift fur medizinische Laboratoriumsdiagnostik","volume":"32 3-4","pages":"209-12"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12881259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Determination of thyroglobulin autoantibodies using enzyme immunoassay, radioimmunoassay and passive hemagglutination]. [用酶免疫分析法、放射免疫分析法和被动血凝法测定甲状腺球蛋白自身抗体]。
Zeitschrift fur medizinische Laboratoriumsdiagnostik Pub Date : 1991-01-01
H Struy, G Horeschi, M Zugehör, J Morenz
{"title":"[Determination of thyroglobulin autoantibodies using enzyme immunoassay, radioimmunoassay and passive hemagglutination].","authors":"H Struy,&nbsp;G Horeschi,&nbsp;M Zugehör,&nbsp;J Morenz","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A solid-phase enzyme immunoassay, a radioimmunoassay and the passive hemagglutination were tested for the determination of antithyroglobulin autoantibodies. The antibody-titres detected with these methods showed a good correlation. The sensitivities of the solid-phase immunoassays are four times higher than the sensitivity of the passive hemagglutination. For the diagnosis of thyroid autoimmune diseases the enzyme immunoassay.</p>","PeriodicalId":76852,"journal":{"name":"Zeitschrift fur medizinische Laboratoriumsdiagnostik","volume":"32 1","pages":"9-14"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13224983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Molecular-biological aspects of diabetes mellitus]. [糖尿病的分子生物学方面]。
Zeitschrift fur medizinische Laboratoriumsdiagnostik Pub Date : 1991-01-01
R Walther, R Brandt, I Leibiger, B Leibiger, S Menzel, C Neumer, H Zühlke
{"title":"[Molecular-biological aspects of diabetes mellitus].","authors":"R Walther,&nbsp;R Brandt,&nbsp;I Leibiger,&nbsp;B Leibiger,&nbsp;S Menzel,&nbsp;C Neumer,&nbsp;H Zühlke","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":76852,"journal":{"name":"Zeitschrift fur medizinische Laboratoriumsdiagnostik","volume":"32 3-4","pages":"124-33"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12844848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Measurement of spontaneous platelet aggregation in citrated whole blood by application of the hematologic automatic Coulter S plus VI]. [应用血液学自动库尔特S + VI测定柠檬酸全血中自发血小板聚集]。
Zeitschrift fur medizinische Laboratoriumsdiagnostik Pub Date : 1991-01-01
R Kätzel, W Lösche, B Selle
{"title":"[Measurement of spontaneous platelet aggregation in citrated whole blood by application of the hematologic automatic Coulter S plus VI].","authors":"R Kätzel,&nbsp;W Lösche,&nbsp;B Selle","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A method for measurement of the spontaneous platelet aggregation in citrated whole blood (SPAV) was tested using the Coulter Counter S plus VI and a simple mechanism for sample rotation. The underlying principle of the procedure consists in the sequential measurement of the decrease of free platelets in a specimen of citrated whole blood which ist rotated at 37 degrees C for 20 minutes. SPAV offers advantages compared to other methods for spontaneous aggregation in platelet rich plasma. First experiences about application of the SPAV-procedure are presented.</p>","PeriodicalId":76852,"journal":{"name":"Zeitschrift fur medizinische Laboratoriumsdiagnostik","volume":"32 1","pages":"44-9"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13224455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Some problems in standardization of fructosamine tests]. 果糖胺检验标准化中的几个问题
Zeitschrift fur medizinische Laboratoriumsdiagnostik Pub Date : 1991-01-01
S Krantz, S Lober, M Lober
{"title":"[Some problems in standardization of fructosamine tests].","authors":"S Krantz,&nbsp;S Lober,&nbsp;M Lober","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The degree of nonenzymatic glycation of serum proteins was estimated in 500 nondiabetic subjects and in 124 Type 2 diabetic out-patients. The values were evaluated in relation to a DMF calibration curve, a secondary glycated protein standard and to serum protein concentrations. Neglecting the evaluation with respect to the protein concentration 0.4% of the samples from nondiabetics and 9% from diabetic patients would have been incorrectly interpreted. The fructosamine values from nondiabetic subjects showed a Gaussian distribution. The use of secondary protein standards is a premise to a widespread application of the fructosamine assay in diabetic care. The calibration of such secondary glycated protein standards results in some problems when using DMF protein mixtures as primary standards, because albumin preparations from the same or different suppliers gave variable standardization curves.</p>","PeriodicalId":76852,"journal":{"name":"Zeitschrift fur medizinische Laboratoriumsdiagnostik","volume":"32 1","pages":"30-7"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13224454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Clinical significance of serum concentration of connective tissue components in cases of organic fibroses]. 【器质性纤维化患者血清结缔组织成分浓度的临床意义】。
Zeitschrift fur medizinische Laboratoriumsdiagnostik Pub Date : 1991-01-01
J Kropf, A M Gressner
{"title":"[Clinical significance of serum concentration of connective tissue components in cases of organic fibroses].","authors":"J Kropf,&nbsp;A M Gressner","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":76852,"journal":{"name":"Zeitschrift fur medizinische Laboratoriumsdiagnostik","volume":"32 3-4","pages":"150-8"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13056873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Use of a computer in a clinical-toxicological laboratory with a fuzzy search]. [在临床毒理学实验室使用计算机进行模糊搜索]。
Zeitschrift fur medizinische Laboratoriumsdiagnostik Pub Date : 1991-01-01
J Gehrke, C Haring, H König, H Marin, H Schmidt, G Stolze
{"title":"[Use of a computer in a clinical-toxicological laboratory with a fuzzy search].","authors":"J Gehrke,&nbsp;C Haring,&nbsp;H König,&nbsp;H Marin,&nbsp;H Schmidt,&nbsp;G Stolze","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>If a clinician is faced with acute intoxications a rapid toxicological test is essential. The toxic substances are identified by a combined use of various systems of analysis such as gas chromatography, thin-layer chromatography or UV-spectroscopy. As the result of the analysis a data pattern is obtained which can be interpreted by comparing it with substance-related reference patterns. This paper describes a computer-program which allows more efficient reference comparisons and which gives a support of the decisions. The algorithms of the comparisions are based on fuzzy-theory.</p>","PeriodicalId":76852,"journal":{"name":"Zeitschrift fur medizinische Laboratoriumsdiagnostik","volume":"32 3-4","pages":"197-203"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13056881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[A program for computer-supported immunoassay analysis]. [计算机支持的免疫分析程序]。
Zeitschrift fur medizinische Laboratoriumsdiagnostik Pub Date : 1991-01-01
J Gabert, G Uhlmann
{"title":"[A program for computer-supported immunoassay analysis].","authors":"J Gabert,&nbsp;G Uhlmann","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":76852,"journal":{"name":"Zeitschrift fur medizinische Laboratoriumsdiagnostik","volume":"32 1","pages":"64"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13224461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Analysis of hyaluronic acid in punctates in biochemical functional tests of joints]. 关节生化功能试验中透明质酸含量分析。
Zeitschrift fur medizinische Laboratoriumsdiagnostik Pub Date : 1991-01-01
R Haupt, H H Heilmann, K Lindenhayn, H Rabe
{"title":"[Analysis of hyaluronic acid in punctates in biochemical functional tests of joints].","authors":"R Haupt,&nbsp;H H Heilmann,&nbsp;K Lindenhayn,&nbsp;H Rabe","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The punctates of the biochemical joint function test contain hydroxyethyl starch which disturbs the assay of uronic acid and glucosamine for the estimation of hyaluronate. Therefore hyaluronate (0.03-0.75 g/l) was estimated by precipitation with Alcian blue on cellulose acetate membranes and measuring of the absorbance at 678 nm after dissolution in aqueous SDS (75 g/l). The gel-filtration HPLC separates the high-molecular-mass hyaluronate from low-molecular-mass impurities of biological samples. By comparing with a standard curve (0.5-2.5 g/l hyaluronate) the concentration can be determined. The results obtained by the two methods were significantly correlated (p less than 0.05). The Alcian blue technique is useful for rapid screening, the HPLC technique to confirm these values.</p>","PeriodicalId":76852,"journal":{"name":"Zeitschrift fur medizinische Laboratoriumsdiagnostik","volume":"32 2","pages":"94-100"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13224992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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