Nihon Ketsueki Gakkai zasshi : journal of Japan Haematological Society最新文献_第6页

Ca2+ influx and platelet membrane glycoproteins. Ca2+内流与血小板膜糖蛋白。

Nihon Ketsueki Gakkai zasshi : journal of Japan Haematological Society Pub Date : 1989-12-01

A Yamaguchi, K Tanoue, H Yamazaki

{"title":"Ca2+ influx and platelet membrane glycoproteins.","authors":"A Yamaguchi, K Tanoue, H Yamazaki","doi":"","DOIUrl":"","url":null,"abstract":"When aequorin-loaded platelets were stimulated with thrombin, the luminescence of aequorin showed two peaks. From experiments with 1 mM external Ca2+ or 1 mM EGTA, both one-half of the first peak and the entire second peak reflected the influx of Ca2+ from the external medium, and the remaining half of the first peak reflected the mobilization of Ca2+ from its storage site. A monoclonal antibody (TM83), that recognizes the GPIIb/IIIa complex which has binding sites for fibrinogen, and synthetic peptide GRGDSP are known to inhibit fibrinogen binding and platelet aggregation. Both of them eliminated the second peak of intracellular free calcium. Similar effects were observed during activation by collagen, but not by TPA. Also dihydrocytochalasin B inhibited the second peak of Ca2+ influx by thrombin, suggesting that the signal, which was caused by fibrinogen-binding to GPIIb/IIIa (aggregation) in thrombin-activated platelets, is transferred to the inner sites of GPIIb/IIIa complex and induces the cytoskeletal reorganization such as actin polymerization. This in turn, induces the secondary increase in [Ca2+] i of platelets. It is interesting that ticlopidine inhibited the Ca2+ influx through the GPIIb/IIIa complex. This result suggests the importance of such kinds of antiplatelet drugs to prevent thrombus formation.","PeriodicalId":76233,"journal":{"name":"Nihon Ketsueki Gakkai zasshi : journal of Japan Haematological Society","volume":"52 8","pages":"1480-8"},"PeriodicalIF":0.0,"publicationDate":"1989-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13772386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aplastic anemia. Immunosuppressive therapy in a multi center trial in Japan. 再生障碍性贫血。免疫抑制疗法在日本的多中心试验。

Nihon Ketsueki Gakkai zasshi : journal of Japan Haematological Society Pub Date : 1989-12-01

K Kitamura, A Urabe

引用次数: 0

A mechanism for cross-lineage gene rearrangements in B-cell neoplasm. b细胞肿瘤中跨谱系基因重排的机制。

Nihon Ketsueki Gakkai zasshi : journal of Japan Haematological Society Pub Date : 1989-12-01

H Miwa, K Kita, T Nosaka, K Kawakami, T Ikeda, S Shirakawa

{"title":"A mechanism for cross-lineage gene rearrangements in B-cell neoplasm.","authors":"H Miwa, K Kita, T Nosaka, K Kawakami, T Ikeda, S Shirakawa","doi":"","DOIUrl":"","url":null,"abstract":"The organization of immunoglobulin heavy chain (IgH) gene of B precursor cell acute lymphoblastic leukemia (ALL) was examined in order to elucidate the mechanism causing simultaneous TCR gene rearrangements. Study using a 5'D probe lying 20 kb upstream of IgH D region genes was useful to distinguish diversity(D)-joining(J) recombination(DJ) from variable(V)-DJ recombination(VDJ). Indeed, IgH gene structures determined by 5'D study well correlated to those recognized by DJ or VDJ transcripts. IgH gene rearrangements of B precursor cell ALL showed developmentally restricted gene recombination; DJ/DJ genotype in the most immature stage and VDJ/VDJ genotype in the relatively mature stage. B precursor cell ALL with simultaneous rearrangements were frequently found in relatively mature cells, i.e., CD20 expressing cells on their surface. Furthermore, most of such dual genotypic ALL showed that at least one allele of IgH genes was VDJ recombination. This finding suggests that dual genotype was the incidental product by a putative common recombinase during the process of VH gene rearrangements. Moreover, since IgH gene rearrangements of acute unclassified leukemia with dual genotype were DQ52 genotype, which indicates abortive gene rearrangements, it was also thought that dual genotype occurred due to the pluripotentiality of the stem cell.","PeriodicalId":76233,"journal":{"name":"Nihon Ketsueki Gakkai zasshi : journal of Japan Haematological Society","volume":"52 8","pages":"1442-50"},"PeriodicalIF":0.0,"publicationDate":"1989-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13664941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular genetic approach to the analysis of clonal proliferation in hematologic disorders. 血液病克隆增殖的分子遗传学分析。

Nihon Ketsueki Gakkai zasshi : journal of Japan Haematological Society Pub Date : 1989-12-01

K Morita, N Tsukamoto, M Karasawa, M Omine

引用次数: 0

Bone marrow transplantation for patients with severe aplastic anemia and myelodysplastic syndrome. 骨髓移植治疗严重再生障碍性贫血和骨髓增生异常综合征。

Nihon Ketsueki Gakkai zasshi : journal of Japan Haematological Society Pub Date : 1989-12-01

S Kai, Y Shinohara

引用次数: 0

Prognosis of chronic thrombocytopenic purpura. 慢性血小板减少性紫癜的预后。

Nihon Ketsueki Gakkai zasshi : journal of Japan Haematological Society Pub Date : 1989-12-01

S Kuriya, T Nomura

引用次数: 0

Analysis of gene amplification in human tumor cells by in-gel DNA renaturation assay. 凝胶DNA再生法分析人肿瘤细胞基因扩增。

Nihon Ketsueki Gakkai zasshi : journal of Japan Haematological Society Pub Date : 1989-12-01

M Fukumoto, H Tashiro, I B Roninson

引用次数: 0

The signal transduction systems and intracellular Ca2+ dynamics in arachidonate-induced platelet activation. 花生四烯酸盐诱导血小板活化的信号转导系统和细胞内Ca2+动力学。

Nihon Ketsueki Gakkai zasshi : journal of Japan Haematological Society Pub Date : 1989-12-01

M Nishikawa, F Komada, Y Uemura, H Hidaka, S Shirakawa

{"title":"The signal transduction systems and intracellular Ca2+ dynamics in arachidonate-induced platelet activation.","authors":"M Nishikawa, F Komada, Y Uemura, H Hidaka, S Shirakawa","doi":"","DOIUrl":"","url":null,"abstract":"Types II and III protein kinase C were expressed in human platelets and showed slightly different modes of activation and kinetic properties. Type III isozyme was more sensitive than type II for the activation of each isozyme with arachidonate (AA) although both isozymes were activated by diacylglycerol and phosphatidylserine in a similar manner. When human platelets were stimulated by AA, two types of platelet activation, a low level of AA (0.1-2.5 micrograms/ml)- and a high level of AA (10-50 micrograms/ml)-induced activations, were observed. These activations were associated with the phosphorylation of 40K and 20K proteins. Although a low level of AA (0.45-10.0 micrograms/ml) induced the formation of [32P] phosphatidate in intact platelets prelabeled with [32P] Pi, AA at high concentrations (20-50 micrograms/ml) did not stimulate phospholipase C. The incubation of fura 2 loaded platelets with a low level of AA evoked a rapid and transient elevation in [Ca2+] i. In contrast, a high level of AA induced an irreversible increase in [Ca2+] i but this [Ca2+] i elevation alone was not associated with platelet activation. These results suggest that the signal transduction system by a high level of AA on human platelets is different from that seen with a low level of AA. A high level of AA induces platelet activation, without phospholipase C stimulation, and therefore, the ability of AA to directly activate protein kinase C (pre-dominantly type III isozyme) may contribute toward the activation of platelets by a high level of AA.","PeriodicalId":76233,"journal":{"name":"Nihon Ketsueki Gakkai zasshi : journal of Japan Haematological Society","volume":"52 8","pages":"1489-97"},"PeriodicalIF":0.0,"publicationDate":"1989-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13663340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of complex formation and epidermal growth factor-like domains in the regulation of blood coagulation by the thrombomodulin-protein C system. 复合物的形成和表皮生长因子样结构域在凝血调节蛋白C系统中的作用。

Nihon Ketsueki Gakkai zasshi : journal of Japan Haematological Society Pub Date : 1989-12-01

S Kurosawa

{"title":"The role of complex formation and epidermal growth factor-like domains in the regulation of blood coagulation by the thrombomodulin-protein C system.","authors":"S Kurosawa","doi":"","DOIUrl":"","url":null,"abstract":"The explosive nature of the coagulation cascade led many scientists to investigate how it is regulated. Proteinase inhibitors such as antithrombin III inhibit active proteases of the coagulation cascade. Cofactors such as factor Va and factor VIIIa are proteolytically inactivated by activated protein C. Protein C is activated by the thrombin-thrombomodulin complex on the endothelial cell surface. Thus, the independent actions of the proteinase inhibitor system and the thrombomodulin-protein C system complement each other to maintain regulation of blood coagulation. The thrombin binding site of thrombomodulin was identified to be the fifth and sixth repeats of the epidermal growth factor-like domain. The same binding template contains sufficient information to block the functions of thrombin as a procoagulant. However, additional repeats are required for the activation of protein C. Thrombomodulin is the first example which illustrates that the epidermal growth factor-like domain functions as a binding template for thrombin and as a switch to turn off the procoagulant activity of thrombin as well as to trigger the protein C anticoagulant pathway. Epidermal growth factor-like structures are found in many of the coagulation factors. Complex formation is a repeated theme not only in the blood coagulation cascade but also in the thrombomodulin-protein C anticoagulant pathway.","PeriodicalId":76233,"journal":{"name":"Nihon Ketsueki Gakkai zasshi : journal of Japan Haematological Society","volume":"52 8","pages":"1343-9"},"PeriodicalIF":0.0,"publicationDate":"1989-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13703353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chronic B cell lymphocytic leukemias: diagnosis and cytologic characteristics of their cells. 慢性B细胞淋巴细胞白血病的诊断及其细胞的细胞学特征。

Nihon Ketsueki Gakkai zasshi : journal of Japan Haematological Society Pub Date : 1989-12-01

T Kitani

引用次数: 0