Nihon Ketsueki Gakkai zasshi : journal of Japan Haematological Society最新文献_第10页

[Burst promoting activity of lymphocytes from uremic patients]. [尿毒症患者淋巴细胞促爆发活性]。

Nihon Ketsueki Gakkai zasshi : journal of Japan Haematological Society Pub Date : 1989-08-01

Y Kawano

引用次数: 0

[HTLV-I carriers in ATL/L-nonendemic area--with reference to blood transfusions]. [ATL/ l非流行地区HTLV-I携带者-参照输血情况]。

Nihon Ketsueki Gakkai zasshi : journal of Japan Haematological Society Pub Date : 1989-08-01

M Sakuma, T Takiguchi, S Konda

{"title":"[HTLV-I carriers in ATL/L-nonendemic area--with reference to blood transfusions].","authors":"M Sakuma, T Takiguchi, S Konda","doi":"","DOIUrl":"","url":null,"abstract":"Sera from 5,705 persons were tested for antibodies to human T-cell leukemia virus type 1 (HTLV-I) by gelatin particle agglutination test (PA) and enzyme immunoassay (EIA) as screening methods, and the antibodies were confirmed by indirect immunofluorescence assay (IF) and Western blotting method (WB). The antibodies were detected in 30 (1.34%) of 2,239 patients who had received blood transfusions, 3 (0.29%) of 1,022 healthy blood donors, all 18 adult T-cell leukemia/lymphoma (ATL/L) patients, 23 (47%) of 49 family members of the ATL/L patients and 10 (0.53%) of the other 1,898 persons. Before the introduction of the mass screening of healthy blood donors by the Japan Red Cross Blood Center, 17 (2.29%) of 743 recipients tested were positive for the antibodies by PA and most of them were confirmed by IF and WB. Ten of these 17 recipients tested were negative for the antibodies before the blood transfusions. After the introduction of mass screening, 13 (0.87%) of 1,496 recipients tested were positive for the antibodies by PA, but the seroconversion of the antibodies by blood transfusions could not be confirmed clearly. In persons with low titers (1:16-1:64) in PA, the coincident rate of positive reaction with EIA, IF and WB was only 9.5%, 0.0% and 23.8%, respectively, and most of the immunoglobulin (Ig) class was IgM. On the other hand, in those with titers of 1:128 or greater in PA, the coincident rate was 72.7%, 81.8% and 90.9%, respectively, and the Ig class was IgG or IgG + IgM.","PeriodicalId":76233,"journal":{"name":"Nihon Ketsueki Gakkai zasshi : journal of Japan Haematological Society","volume":"52 5","pages":"869-79"},"PeriodicalIF":0.0,"publicationDate":"1989-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13731426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Spontaneous remission of four months' duration in hypoplastic leukemia with tetraploid chromosome after blood transfusions and infection]. 【四倍体染色体发育不全白血病经输血和感染后4个月时间自行缓解】。

Nihon Ketsueki Gakkai zasshi : journal of Japan Haematological Society Pub Date : 1989-08-01

T Maekawa, H Fujii, S Horiike, T Okuda, S Yokota, K Ueda, Y Urata

引用次数: 0

[Analysis of platelet surface conformation in thrombin-induced aggregation]. [凝血酶诱导聚集的血小板表面构象分析]。

Nihon Ketsueki Gakkai zasshi : journal of Japan Haematological Society Pub Date : 1989-08-01

S Nomura, H Nagata, M Yanabu, M Suzuki, T Soga, S Ohga, K Kondo, N Sone, C Kitada, H Kitajima

{"title":"[Analysis of platelet surface conformation in thrombin-induced aggregation].","authors":"S Nomura, H Nagata, M Yanabu, M Suzuki, T Soga, S Ohga, K Kondo, N Sone, C Kitada, H Kitajima","doi":"","DOIUrl":"","url":null,"abstract":"We used flow cytometry to investigate the change of platelet membrane glycoproteins (GPIb and GP IIb/IIIa) and the distributions of fibrinogen (Fbg), thrombospondin (TSP) and fibronectin (Fn) on the surface of thrombin-stimulated platelets. The binding of a monoclonal antibody directed at the von Willebrand factor binding site on GPIb decreased in thrombin-stimulated platelets. This antibody caused a reactive delay in thrombin-induced aggregation, but had little influence on aggregability. Slight thrombin-induced aggregation was observed even after blocking the binding of Fbg to GP II b/IIIa. The new expression of GP II b/IIIa was detected on the surface of thrombin-stimulated platelets, whereas there was little increase of Fbg dependent on this GP II b/IIIa. An increase of TSP after thrombin stimulation was observed on the surface of platelets of healthy controls and patients with Glanzmann's thrombasthenia (Type I). The level of on platelet surface was slightly increased by thrombin stimulation. The mechanism involved in thrombin-induced aggregation appears to differ from that in ADP-induced aggregation.","PeriodicalId":76233,"journal":{"name":"Nihon Ketsueki Gakkai zasshi : journal of Japan Haematological Society","volume":"52 5","pages":"895-905"},"PeriodicalIF":0.0,"publicationDate":"1989-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13732695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Mechanisms of human platelet aggregation and release reaction induced by influenza virus]. 流感病毒诱导人血小板聚集和释放反应的机制

Nihon Ketsueki Gakkai zasshi : journal of Japan Haematological Society Pub Date : 1989-08-01

S Hagiwara

引用次数: 0

[Immunochemical characterization of platelet-specific alloantigen Baka]. [血小板特异性同种异体抗原Baka的免疫化学表征]。

Nihon Ketsueki Gakkai zasshi : journal of Japan Haematological Society Pub Date : 1989-08-01

Y Tomiyama, Y Kurata, Y Shibata, H Take, T Furubayashi, T Tsubakio, T Yonezawa, S Tarui

引用次数: 0

[Clonal response of murine myelomonocytic leukemia cells (WEHI-3B-Y1) to purified recombinant human hemopoietic factors in serum-free culture]. [小鼠髓单细胞白血病细胞(WEHI-3B-Y1)对纯化重组人造血因子的无血清培养克隆反应]。

Nihon Ketsueki Gakkai zasshi : journal of Japan Haematological Society Pub Date : 1989-08-01

Y Kajigaya, H Sasaki, K Ikuta, S Matsuyama

引用次数: 0

[Alloreactivity and mitogen-induced responses in allogeneic murine bone marrow chimeras]. [同种异体小鼠骨髓嵌合体的同种异体反应性和丝裂原诱导的反应]。

Nihon Ketsueki Gakkai zasshi : journal of Japan Haematological Society Pub Date : 1989-08-01

H Fujimoto

{"title":"[Alloreactivity and mitogen-induced responses in allogeneic murine bone marrow chimeras].","authors":"H Fujimoto","doi":"","DOIUrl":"","url":null,"abstract":"Alloreactive mixed lymphocyte reaction (MLR), mitogen-induced response (MR), and suppressor cells against these responses in murine bone marrow chimeras were examined, to clarify the mechanisms of immunological tolerance and immunodeficiency after bone marrow transplantation (BMT). Between 35 and 70 days after BMT, there was no response of spleen cells from allogeneic chimeras against host (C3H/He) and donor (BALB/c) cells, although responses against third party (C57BL/6) cells were detected, thus indicating that these allogeneic chimeras were immunologically tolerant. The activity of suppressor cells against alloreactive responses was increased 35 to 55 days after BMT, so that these suppressor cells appeared to be related to immunological tolerance. Some of the suppressor cells against alloreactive responses were Thy1+. Among them some were Lyt1+ or Lyt2+, and others were Lyt1+2+. Plastic dish non-adherent cells had slightly weaker suppressor activity than adherent cells. Proliferative responses to Con A, PHA, and PWM were decreased 13 to 15 days after BMT, and gradually increased. The responses to LPS differed from those to the former three mitogens, showing an enhanced response 21 to 25 days after BMT. The increased response to LPS did not appear to be simply due to the increased number of non-T cells in the spleen of allogeneic chimeras. The alloantigen specific suppressor cells may play an important role in the induction and maintenance of immunological tolerance, while the alloantigen nonspecific suppressor cells and suppressor cells against MRs may be related to immunodeficiency after BMT.","PeriodicalId":76233,"journal":{"name":"Nihon Ketsueki Gakkai zasshi : journal of Japan Haematological Society","volume":"52 5","pages":"858-68"},"PeriodicalIF":0.0,"publicationDate":"1989-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13675107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Cystine transport and glutathione metabolism in human erythrocytes]. [人红细胞中胱氨酸转运和谷胱甘肽代谢]。

Nihon Ketsueki Gakkai zasshi : journal of Japan Haematological Society Pub Date : 1989-08-01

Y Otsuka

{"title":"[Cystine transport and glutathione metabolism in human erythrocytes].","authors":"Y Otsuka","doi":"","DOIUrl":"","url":null,"abstract":"Cystine was transported into human erythrocytes in the presence of tertiary-butyl hydroperoxide (t-BH) or 1-chloro-2, 4-dinitrobenzene (CDNB). The transport rate of cystine was dependent on the extracellular concentration of t-BH or CDNB, and on the incubation time. By Dowex-1 column chromatography, the transported cystine was incorporated into fractions of glutathione disulfide (GSSG) and glutathione-S (GSH-S) conjugate. Cystine was also transported into reconstituted erythrocyte ghost with GSSG. The transport of cystine was Na+ dependent and decreased in the presence of N-ethylmaleimide, and it was competitively inhibited by DL-homocystine and L-alanine. The inhibition rates by DL-homocystine and L-alanine were 75% and 68%, with similar Ki values of 0.7 mM and 0.6 mM, respectively. The Km value for cystine transport was 0.15 mM. The activity of GSH-cystine transhydrogenase was detected in the hemolysate and this enzyme is thought to catalyze the action of incorporation of cystine into GSH. This enzyme was partially purified from normal human erythrocytes. In the presence of CDNB, similar rates of cystine transport were observed among the diabetic patients (n = 11), hypoxemic patients (n = 10) and the control subjects (n = 20). It is suggested that cystine transport is induced for glutathione synthesis when human erythrocytes are exposed to oxidative stresses.","PeriodicalId":76233,"journal":{"name":"Nihon Ketsueki Gakkai zasshi : journal of Japan Haematological Society","volume":"52 5","pages":"823-30"},"PeriodicalIF":0.0,"publicationDate":"1989-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13731422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intracytoplasmic localization of CD3 antigen in NKH1+ azurophilic granular T-lymphoblastic lymphoma cells. CD3抗原在NKH1+嗜氮颗粒t淋巴母细胞淋巴瘤细胞浆内的定位。

Nihon Ketsueki Gakkai zasshi : journal of Japan Haematological Society Pub Date : 1989-07-01

Y Yoda, N Mori, K Oka, M Nakazawa, R Mukai, T Abe

{"title":"Intracytoplasmic localization of CD3 antigen in NKH1+ azurophilic granular T-lymphoblastic lymphoma cells.","authors":"Y Yoda, N Mori, K Oka, M Nakazawa, R Mukai, T Abe","doi":"","DOIUrl":"","url":null,"abstract":"The intracytoplasmic localization of CD3 antigen was examined in azurophilic granular T-lymphoblastic lymphoma cells obtained from a 27-year-old female. Examination by flow cytometry and an immunohistological method revealed that the surface phenotype of the lymphoma cells were NKH1+, CD5+, CD7+, CD1-, CD2-, CD4-, CD8- and CD16-. CD3 was not detected on the cell-surface by flow cytometry but was found in the cytoplasm using the immunohistological method. CD3 was detected in the perinuclear space and rough endoplasmic reticulum (rough ER) by an immunoelectron microscopic study. An electron microscopic observation of the granules revealed lysosomal structures with low electron density. The lymphoma cells proliferated when cultured in the presence of interleukin-2 but showed no NK (K562) activity before and after culture. The molecular size of metabolically labeled intracytoplasmic CD3 precipitated by Leu 4 monoclonal antibody was identical to that of normal CD3. These results indicate that the lymphoma cells are neoplastic counterparts of immature thymocyte at a very early stage of differentiation and may be related to the lineage of CD3+ NKH1+ T-lymphocyte.","PeriodicalId":76233,"journal":{"name":"Nihon Ketsueki Gakkai zasshi : journal of Japan Haematological Society","volume":"52 4","pages":"740-8"},"PeriodicalIF":0.0,"publicationDate":"1989-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13677574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0