Nihon Ketsueki Gakkai zasshi : journal of Japan Haematological Society最新文献

The hematopoietic system 造血系统

Nihon Ketsueki Gakkai zasshi : journal of Japan Haematological Society Pub Date : 2012-08-01 DOI: 10.3109/9781841849232.010

K. Burchard

引用次数: 205

[Flow cytometric analysis of immunophagocytosis using sensitized fluorescent microspheres bearing C3b]. [使用含C3b的致敏荧光微球进行免疫吞噬作用的流式细胞分析]。

Nihon Ketsueki Gakkai zasshi : journal of Japan Haematological Society Pub Date : 1990-05-01

A Andoh, Y Fujiyama, S Hirotani, K Hodohara, T Bamba, S Hosoda

引用次数: 0

[Mechanism of hyperferritinemia in a case of malignant histiocytosis]. [恶性组织细胞增多症一例高铁蛋白血症的发病机制]。

Nihon Ketsueki Gakkai zasshi : journal of Japan Haematological Society Pub Date : 1990-05-01

K Miyazawa, M Shiota, Y Takakuwa, Y Kawanishi, H Iwabuchi, M Nakano, O Yoshikawa, K Toyama, H Serizawa, H Nagasawa

{"title":"[Mechanism of hyperferritinemia in a case of malignant histiocytosis].","authors":"K Miyazawa, M Shiota, Y Takakuwa, Y Kawanishi, H Iwabuchi, M Nakano, O Yoshikawa, K Toyama, H Serizawa, H Nagasawa","doi":"","DOIUrl":"","url":null,"abstract":"We report a case of malignant histiocytosis diagnosed by liver-spleen biopsy under laparoscopy. A 49-year-old woman was admitted to our hospital with thrombocytopenia, moderate anemia and hypoproteinemia. Her bone marrow findings revealed erythroid and megakaryocyte hyperplasia, and the serum ferritin concentration was 2,250 ng/ml though she had not received any blood transfusions. Ferrokinetics analysis showed the pattern of ineffective erythropoiesis, and the half-lives of erythrocytes and platelets were both shortened. Her hepatosplenomegaly gradually increased accompanied by increasing serum ferritin level to 10,000 ng/ml. Liver-spleen biopsy was carried out under laparoscopy and revealed infiltration of atypical histiocytes with erythrophagocytosis, which were positive for S-100 and ferritin but negative for lysozyme. The rate of glycosylation in whole serum ferritin, analyzed by using concanavalin-A binding method, showed that her glycosylated ferritin content was only 8.3%, whereas in sera after iron overloading, it was about 70%. Serum isoferritin profiles by isoelectric focussing were studied, and isoferritin pattern from malignant histiocytosis was the same as that in iron overloading after neuraminidase treatment. These findings suggest that serum ferritin is synthesized in proliferating histiocytes and released in the plasma as a nonsecretory type (nonglycosylated ferritin) in this case.","PeriodicalId":76233,"journal":{"name":"Nihon Ketsueki Gakkai zasshi : journal of Japan Haematological Society","volume":"53 3","pages":"575-81"},"PeriodicalIF":0.0,"publicationDate":"1990-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13535761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Production and secretion of BSF2/IL6 in a case of hairy cell leukemia with polyclonal hypergammaglobulinemia]. [1例毛细胞白血病合并多克隆高γ球蛋白血症的BSF2/IL6的产生和分泌]。

Nihon Ketsueki Gakkai zasshi : journal of Japan Haematological Society Pub Date : 1990-05-01

M Shiota, Y Kawanishi, M Nakano, T Ohdo, K Toyama

{"title":"[Production and secretion of BSF2/IL6 in a case of hairy cell leukemia with polyclonal hypergammaglobulinemia].","authors":"M Shiota, Y Kawanishi, M Nakano, T Ohdo, K Toyama","doi":"","DOIUrl":"","url":null,"abstract":"A 55-year-old woman was first seen in October 1986, because of splenomegaly, moderate anemia and leukocytosis. The hemoglobin was 8.8 g/dl, platelet count 24.4 X 10(4)/microliters, and the white cell count 23,800/microliters with 73% atypical lymphoid cells. The bone marrow nucleated cell count was 99,000/microliters with 36% lymphoid cells. These atypical lymphoid cells showed hairy appearance under phase-contrast microscopy, and were positive for tartrate-resistant acid phosphatase. These cells showed the surface phenotype of CD10, CD19, CD20, Leu M5, HCM, and IgG K. Biochemical data revealed marked polyclonal hypergammagloburinemia (PHG) of IgG type (IgG 8756 mg/dl). To elucidate the mechanism of the PHG, we investigated whether hairy cells produce interleukin 6 (IL-6) and express IL-6 receptor. The culture supernatant of these hairy cells increased 3H-thymidine uptake of a IL-6 dependent hybridoma clone (MH60) in a dose-dependent manner. These cells were stained with anti-IL-6 antibody using immuno-cytochemical technique. Our results suggested that these hairy cells produce and secrete IL-6. Immunocytochemical staining with anti IL-6 receptor antibody and the binding assay with 125I-labelled recombinant IL-6 revealed that these cells express little or no receptors for IL-6. It was therefore suggested that IL-6 produced by hairy cells in this case is not an autocrine growth factor for these cells but may play a role in development of PHG by stimulating normal B lymphocytes to produce an excessive amount of immunoglobulin.","PeriodicalId":76233,"journal":{"name":"Nihon Ketsueki Gakkai zasshi : journal of Japan Haematological Society","volume":"53 3","pages":"582-8"},"PeriodicalIF":0.0,"publicationDate":"1990-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13534324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Long-term cultures of marrow cells from the patients with aplastic anemia]. 再生障碍性贫血患者骨髓细胞的长期培养。

Nihon Ketsueki Gakkai zasshi : journal of Japan Haematological Society Pub Date : 1990-05-01

M Fujiwara

引用次数: 0

[Application of nucleolar organizer region staining technique to air-dried blood smears]. [核仁组织区染色技术在风干血涂片中的应用]。

Nihon Ketsueki Gakkai zasshi : journal of Japan Haematological Society Pub Date : 1990-05-01

S Nakamura, Y Takeda, T Yoshida, S Ohtake, K Kobayashi, M Kanno, S Matano, T Matsuda

{"title":"[Application of nucleolar organizer region staining technique to air-dried blood smears].","authors":"S Nakamura, Y Takeda, T Yoshida, S Ohtake, K Kobayashi, M Kanno, S Matano, T Matsuda","doi":"","DOIUrl":"","url":null,"abstract":"Silver staining of nucleolar organizer regions (NOR) was applied to air-dried peripheral and bone marrow smears of normal subjects and leukemic patients. Specimens were fixed in buffered acetone formalin. Even in smears kept for 2 years at room temperature, the stainability of Ag-NOR was well preserved. By dipping Giemsa-stained smears in 5% trichloracetic acid and then placing them in methanol for 5 minutes, the stain was leached out. After the dye had been removed, the smears were clearly stained by a Ag-NOR staining technique. The mean number of Ag-NOR per nucleus of mature granulocytes and mononuclear cells in normal peripheral bloods was 0.59 and 1.43 respectively. The mean number of Ag-NOR per nucleus of peripheral and bone marrow leukemic cells from patients with acute leukemia and chronic myelocytic leukemia in blastic crisis was 2.32 and 2.66 respectively. On the other hand, the mean number of Ag-NOR per nucleus of peripheral leukemic cells from patients with chronic lymphocytic leukemia was 1.48. These results suggest that acute leukemia cells possess a more active proliferating potential. The Ag-NOR staining technique is very simple and might be useful for investigation of hematologic cells.","PeriodicalId":76233,"journal":{"name":"Nihon Ketsueki Gakkai zasshi : journal of Japan Haematological Society","volume":"53 3","pages":"559-66"},"PeriodicalIF":0.0,"publicationDate":"1990-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12861179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Autoinduction of differentiation in human myelocytic leukemia cells (HL-60-Y3)]. [人髓细胞白血病细胞(HL-60-Y3)的自我诱导分化]。

Nihon Ketsueki Gakkai zasshi : journal of Japan Haematological Society Pub Date : 1990-05-01

Y Kajigaya

引用次数: 0

[Biochemical and molecular biological studies on beta-glucuronidase in myelogenous leukemic cells]. [髓系白血病细胞β -葡萄糖醛酸酶的生化和分子生物学研究]。

Nihon Ketsueki Gakkai zasshi : journal of Japan Haematological Society Pub Date : 1990-05-01

J Tanaka

{"title":"[Biochemical and molecular biological studies on beta-glucuronidase in myelogenous leukemic cells].","authors":"J Tanaka","doi":"","DOIUrl":"","url":null,"abstract":"beta-Glucuronidase purified from normal human tissues (placenta, liver and spleen) and granulocytes was composed of 80 kilo-Dalton (kDa), 64 kDa and 18 kDa subunits. The enzyme from human myelogenous leukemic cells contained a 80 kDa subunit as a major component. Amino acid sequencing revealed that N-terminal regions of the 80 kDa from CML cells and the 18 kDa from placenta were identical, and the sequence of the 64 kDa from placenta was identical to the downstream sequence of Gly138 in the 80 kDa enzyme. Therefore, it is probable that the 80 kDa is a precursor form and the 64 kDa is a mature form that is derived by removal of N-terminal 18 kDa peptide (137 amino acids) from the precursor form. These observation simply that proteolytic processing of this enzyme is impaired in myelogenous leukemic cells. The possibility of impaired protease activity against the 80 kDa subunit in CML cells was excluded, since the 80 kDa was not susceptible to several protease sources from normal tissues (placenta and liver) and granulocytes under a variety of reaction conditions. In addition no mutation of the primary structure in myelogenous leukemic cells was detected through analysis of DNA encoding for a peptide, including Gly138 of the enzyme. Therefore, it is suggested that abnormal processing of beta-GUase in the myelogenous leukemic cells is due to alteration in transcriptional or translational step.","PeriodicalId":76233,"journal":{"name":"Nihon Ketsueki Gakkai zasshi : journal of Japan Haematological Society","volume":"53 3","pages":"549-58"},"PeriodicalIF":0.0,"publicationDate":"1990-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13354572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Proceedings of the 52d general meeting of the Japan Hematologic Society. Tokyo, 29-31 March 1990. Abstracts]. [日本血液学学会第52届大会论文集]1990年3月29日至31日，东京。摘要]。

Nihon Ketsueki Gakkai zasshi : journal of Japan Haematological Society Pub Date : 1990-03-01

引用次数: 0

Clinical usefulness of vinca alkaloid slow infusion in the treatment of chronic refractory idiopathic thrombocytopenic purpura: a multicenter cooperative study. 长春花生物碱缓慢输注治疗慢性难治性特发性血小板减少性紫癜的临床疗效:一项多中心合作研究。

Nihon Ketsueki Gakkai zasshi : journal of Japan Haematological Society Pub Date : 1990-02-01

T Nomura, T Maekawa, H Uchino, T Miyazaki, Y Miura, T Abe, S Asano, S Kuriya, K Nagai, Y Yawata

{"title":"Clinical usefulness of vinca alkaloid slow infusion in the treatment of chronic refractory idiopathic thrombocytopenic purpura: a multicenter cooperative study.","authors":"T Nomura, T Maekawa, H Uchino, T Miyazaki, Y Miura, T Abe, S Asano, S Kuriya, K Nagai, Y Yawata","doi":"","DOIUrl":"","url":null,"abstract":"Thirty-eight patients with chronic refractory idiopathic thrombocytopenic purpura (ITP) were treated with weekly slow infusions of vincristine (0.02 to 0.04 mg/kg) or vinblastine (0.1 to 0.2 mg/kg). Twenty-two patients showed good to excellent responses after one to eight infusions. These responses were generally short, and lasted only in six patients after discontinuance of the therapy. The efficacy was comparable between vincristine and vinblastine. Neither the age, sex, duration of the disease, prior splenectomy nor combined use of adrenocortical steroids was likely to have influenced the therapeutic effect. Side effects such as peripheral neuropathy, alopecia, gastrointestinal symptoms and leukopenia occurred in 34 patients, and necessitated discontinuance of the therapy in eight patients. Slow infusions of vinca alkaloids can be an effective means of inducing platelet response in patients with chronic refractory ITP, but frequent side effects limit its clinical usefulness.","PeriodicalId":76233,"journal":{"name":"Nihon Ketsueki Gakkai zasshi : journal of Japan Haematological Society","volume":"53 1","pages":"98-104"},"PeriodicalIF":0.0,"publicationDate":"1990-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13337379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0