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Vero cytotoxin produced by Escherichia coli strains of animal origin. 由动物源性大肠杆菌菌株产生的Vero细胞毒素。
National Institute of Animal Health quarterly Pub Date : 1981-01-01
M Kashiwazaki, T Ogawa, K Nakamura, Y Isayama, K Tamura, R Sakazaki
{"title":"Vero cytotoxin produced by Escherichia coli strains of animal origin.","authors":"M Kashiwazaki,&nbsp;T Ogawa,&nbsp;K Nakamura,&nbsp;Y Isayama,&nbsp;K Tamura,&nbsp;R Sakazaki","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A heat-labile Vero cytotoxin (VT) was detected by the Vero cell assay in the culture supernatants of ten Escherichia coli strains isolated from a variety of clinical sources. The VT titer varied with strains, ranging from 1:8 to 1:2,048. No VT was found to be cytotoxic for Y1 mouse adrenal cells or to induce fluid accumulation in the rabbit or porcine ileal loops. No strains produced a heat-labile enterotoxin as defined by either tissue culture or the standard ileal loop reaction. Three strains originated from piglets with diarrhea were confirmed by the infant mouse reaction to produce a heat-stable enterotoxin. Neutralization studies revealed antigenically the presence of two different forms of VT produced by E. coli strains of animal origin.</p>","PeriodicalId":76197,"journal":{"name":"National Institute of Animal Health quarterly","volume":"21 2","pages":"68-72"},"PeriodicalIF":0.0,"publicationDate":"1981-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18090839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Isolation of reovirus type 1 from calves in Japan. 日本犊牛呼肠孤病毒1型的分离。
National Institute of Animal Health quarterly Pub Date : 1981-01-01
Y Goto, H Kurogi, Y Fujisaki, Y Ito
{"title":"Isolation of reovirus type 1 from calves in Japan.","authors":"Y Goto,&nbsp;H Kurogi,&nbsp;Y Fujisaki,&nbsp;Y Ito","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":76197,"journal":{"name":"National Institute of Animal Health quarterly","volume":"21 4","pages":"182-3"},"PeriodicalIF":0.0,"publicationDate":"1981-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18351707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
An analysis on the mechanism responsible for genesis of deformation of expiratory-gas-concentration curves in chickens. 鸡呼气气体浓度曲线变形的机理分析。
National Institute of Animal Health quarterly Pub Date : 1981-01-01
T Itabisashi
{"title":"An analysis on the mechanism responsible for genesis of deformation of expiratory-gas-concentration curves in chickens.","authors":"T Itabisashi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Phenomena associated with a deformation of short-time occurrence and noticed on concentration curves of tidal O2 and CO2 were examined in adult hens sitting quietly in a cage or suspended in prone position. During the episode of deformation, a decrease in arterial oxygen-gas tension was apparent. It was associated with a slight increase in arterial carbon-dioxide-gas tension and a slight acidic shift of arterial blood pH. The episode was associated with increases in intrapressure of the interclavicular and bilateral abdominal air sacs. The tracheal expiratory-gas flow changed in pattern. Hypoventilation due to reduction in tidal volume or respiratory rate could not be detected during the episode. The episode was suddenly terminated at the hen's body movement induced spontaneously or by pinching the comb, at coughlike activity or at no obvious change in behavior in a quietly resting position. Deformation similar to the spontaneous one could be induced by 3 ml-water flushing into the trachea or 0.1% methacholine aerosol inhalation. The mechanism responsible for the genesis of deformation was discussed. It was presumed to be based upon some mechanism, such as mucous accumulation in the lung, rather than upon the pulmonary smooth-muscle contraction.</p>","PeriodicalId":76197,"journal":{"name":"National Institute of Animal Health quarterly","volume":"21 2","pages":"94-103"},"PeriodicalIF":0.0,"publicationDate":"1981-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17857298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Role of delayed type hypersensitivity in mice inoculated with Staphylococcus aureus (Strain CH-91). 延迟型超敏反应在接种金黄色葡萄球菌(CH-91株)小鼠中的作用。
National Institute of Animal Health quarterly Pub Date : 1981-01-01
S Takeuchi, Y Nakajima, T Hamaoka, H Minato, T Suto
{"title":"Role of delayed type hypersensitivity in mice inoculated with Staphylococcus aureus (Strain CH-91).","authors":"S Takeuchi,&nbsp;Y Nakajima,&nbsp;T Hamaoka,&nbsp;H Minato,&nbsp;T Suto","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":76197,"journal":{"name":"National Institute of Animal Health quarterly","volume":"21 2","pages":"106-7"},"PeriodicalIF":0.0,"publicationDate":"1981-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18355367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Leg abnormality associated with dietary excessive calcium carbonate in broiler chicks. 饲粮碳酸钙过量与肉鸡腿部畸形的关系。
National Institute of Animal Health quarterly Pub Date : 1981-01-01
Y Ogura
{"title":"Leg abnormality associated with dietary excessive calcium carbonate in broiler chicks.","authors":"Y Ogura","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>When chicks were fed excessive CaCo3, some of them manifested the toes and legs bent in an abnormal direction and final inability to walk in proportion to the dose of calcium. However, chicks fed CaHPO4 containing the same dose of Ca, instead of CaCO3, showed no abnormality of the legs. The leg abnormality of chicks fed excessive CaCO3 resembled the symptom of chicks fed a vitamin D-deficient diet, but there slight differences in serum Ca level and in the findings of the legs by roentgenography between both chick groups. In the radiograph of chicks fed excessive CaCO3, definite radiolucent area were found in all the bone joints related with poor mineralization. Furthermore, a slight elevation of Ca concentration, a decrease in inorganic phosphorous concentration and a remarkable increase in alkaline phosphatase activity were found in the sera of the chicks fed excessive CaCO3. In these chicks were observed the hypertrophic cartilage at the end of bones and an increase in osteoid seams at the diaphysis in the non-decalcified bone section. The findings described above suggested that an excessive Ca supply to chicks might cause a disturbance in P metabolism and produce hypophosphatemic rickets.</p>","PeriodicalId":76197,"journal":{"name":"National Institute of Animal Health quarterly","volume":"21 3","pages":"141-7"},"PeriodicalIF":0.0,"publicationDate":"1981-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18348283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Modification of low virulent Newcastle disease virus infection in chickens infected with reticuloendotheliosis virus. 网状内皮增生病毒感染鸡低毒力新城疫病毒感染的修饰。
National Institute of Animal Health quarterly Pub Date : 1981-01-01
I Yoshida, M Sakata, K Fujita, T Noguchi, N Yuasa
{"title":"Modification of low virulent Newcastle disease virus infection in chickens infected with reticuloendotheliosis virus.","authors":"I Yoshida,&nbsp;M Sakata,&nbsp;K Fujita,&nbsp;T Noguchi,&nbsp;N Yuasa","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>One-day-old SPF chicks were inoculated with reticuloendotheliosis virus (REV) which had been isolated from contaminated Marek's disease vaccine. Then they were subjected to super infection with the B1 or TCND strain of Newcastle disease virus (NDV) and examined for virus recovery, antibody response and the appearance of symptoms. Regardless of the time, from 0 to 8 weeks, of inoculation with the NDV-B1 strain after the REV infection, the antibody response was suppressed and the duration of the NDV recovery prolonged. Specific death preceded by severe respiratory or neural signs occurred more frequently to chicks inoculated with REV than to uninoculated controls after inoculation with the NDV-B1 strain in the neonatal stage or with the NDV-TCND strain at 5 weeks of age.</p>","PeriodicalId":76197,"journal":{"name":"National Institute of Animal Health quarterly","volume":"21 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"1981-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17326580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Direct immunofluorescent staining of Leucocytozoon caulleryi of different developmental stages. 不同发育阶段库勒氏白细胞的直接免疫荧光染色。
National Institute of Animal Health quarterly Pub Date : 1981-01-01
K Fujisaki, H Takamatsu, S Kitaoka, K Suzuki, T Kamio
{"title":"Direct immunofluorescent staining of Leucocytozoon caulleryi of different developmental stages.","authors":"K Fujisaki,&nbsp;H Takamatsu,&nbsp;S Kitaoka,&nbsp;K Suzuki,&nbsp;T Kamio","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Direct immunofluorescence was first used successfully for the staining of Leucocytozoon caulleryi of different developmental stages, such as the 2nd generation schizont and merozoite, gametocyte, zygote, ookinete, and sporozoite. Fluorescent antibody of IgG nature prepared from sera of chickens over a period from the 21st to the 100th day after sporozoite inoculation was used in this experiment. The cross reactivity on different developmental stages of L. caulleryi in the direct fluorescent antibody test suggested that antigens common to different developmental stages, or shared antigens, might exist, as well as specific antigens so far well-known. The fluorescent intensity of oocyst sporozoite, and schizont contents was apparently lower than that of any other stage. This evidence might be elucidated by the antigenic incompletion.</p>","PeriodicalId":76197,"journal":{"name":"National Institute of Animal Health quarterly","volume":"21 2","pages":"73-9"},"PeriodicalIF":0.0,"publicationDate":"1981-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17857297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Glässer's disease in piglets produced by intraperitoneal inoculation with Haemophilus parasuis. 腹腔注射副猪嗜血杆菌引起仔猪Glässer病。
National Institute of Animal Health quarterly Pub Date : 1981-01-01
T Morozumi, T Hiramune, K Kobayashi
{"title":"Glässer's disease in piglets produced by intraperitoneal inoculation with Haemophilus parasuis.","authors":"T Morozumi,&nbsp;T Hiramune,&nbsp;K Kobayashi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Haemophilus parasuis was inoculated into the abdominal cavities of piglets. Its infective doses varied from 1.4 X 10(6) to 1.0 X 10(9) organisms. When inoculated with 1.0 X 10(9) or 1.6 X 10(8) organisms, piglets were affected with fibrinous polyserositis associated with arthritis and purulent meningitis. The three disorders were considered to be characteristic of Glässer's disease. Septicemia was also found in many of the infected piglets, some of which died within a few days after inoculation. No lesions were observed in piglets inoculated with 1.4 X 10(7) or 1.4 X 10(6) organisms. It was difficult to reisolate H.parasuis from lesions, especially those in the abdominal cavities of piglets killed on the 6th day after inoculation. Agglutinating and complement-fixing antibodies were present in the piglets with Glässer's syndrome examined on the 6th day after inoculation. Neither of them was detected in any piglet manifesting no clinical signs, except transient pyrexia.</p>","PeriodicalId":76197,"journal":{"name":"National Institute of Animal Health quarterly","volume":"21 3","pages":"121-8"},"PeriodicalIF":0.0,"publicationDate":"1981-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18345030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Electron microscopy of cells cultured in serum-free medium after inoculation of swine vesicular disease virus. 接种猪水疱病病毒后在无血清培养基中培养细胞的电镜观察。
National Institute of Animal Health quarterly Pub Date : 1981-01-01
M Kubo, M Osada, S Konno, T Saito, M Kodama
{"title":"Electron microscopy of cells cultured in serum-free medium after inoculation of swine vesicular disease virus.","authors":"M Kubo,&nbsp;M Osada,&nbsp;S Konno,&nbsp;T Saito,&nbsp;M Kodama","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Morphological alterations of IB-RS-2 cells cultured in serum-free maintenance medium after inoculation of swine vesicular disease virus (SVDV) were studied electron-microscopically. Cells harvested 0 to 3 hours after inoculation showed no alterations. Cellular alterations were observed from 4 to 7 hours after inoculation. Many vacuoles appeared just beneath the cytoplasmic membrane and were separated by thin cytoplasm. Narrow pathways were sometimes seen in the degenerative cells. They occasionally ran from the vacuole just beneath the cytoplasmic membrane to the nuclear cistern. Ribosome-like granules were seen along the narrow pathway or diffusely in the cytoplasm, or accumulated to form an islet. Membrane-bound bodies were frequently noticed in the central region of the cell. Cytoplasmic blebs were sometimes seen projecting from the cell surface. Vacuoles, narrow pathways and cytoplasmic blebs were absent in cells cultured in the medium with serum. So serum might exert some effects on the cytoplasmic membrane or cytoskeletal framework. Crystalline arrays of SVDV were found in the cytoplasm of degenerative cells harvested from 4 to 7 hours after inoculation. They were near the ribosome-like granules and membrane-bound bodies, but had no relationship with vacuoles just beneath the cytoplasmic membrane and narrow pathway. Their size and morphology were the same as those seen in the cells cultured in the medium with serum.</p>","PeriodicalId":76197,"journal":{"name":"National Institute of Animal Health quarterly","volume":"21 3","pages":"134-40"},"PeriodicalIF":0.0,"publicationDate":"1981-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18345032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Susceptibility of Haemophilus equigenitalis, the causal agent of contagious equine metritis, to 31 antimicrobial agents. 马传染性子宫炎病原菌equigenitalhaemophilus对31种抗微生物药物的敏感性。
National Institute of Animal Health quarterly Pub Date : 1981-01-01
C Sugimoto, Y Isayama, M Kashiwazaki, K Mitani
{"title":"Susceptibility of Haemophilus equigenitalis, the causal agent of contagious equine metritis, to 31 antimicrobial agents.","authors":"C Sugimoto,&nbsp;Y Isayama,&nbsp;M Kashiwazaki,&nbsp;K Mitani","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The minimal inhibitory concentrations of 31 antimicrobial agents were determined for 99 isolates of Haemophilus equigenitalis by the agar dilution method. All the isolates showed good susceptibility to 26 antimicrobial agents tests, minimal inhibitory concentrations of which were less than 3.13 micrograms/ml for more than 90% of the isolates. Of these agents, 4 macrolides (erythromycin, oleandomycin, kitasamycin, tylosin), 3 tetracyclines (tetracycline, chlortetracycline, oxytetracycline), 1 peptide (colistin), 1 penicillin (ampicillin) and 1 pleuromutilin (tiamulin) were the most active agents, showing a minimal inhibitory concentration of less than 0.39 micrograms/ml for more than 90% of the isolates. The growth of more than 90% of the isolates was not inhibited by 800 micrograms/ml of streptomycin.</p>","PeriodicalId":76197,"journal":{"name":"National Institute of Animal Health quarterly","volume":"21 4","pages":"159-62"},"PeriodicalIF":0.0,"publicationDate":"1981-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18355369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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