E Kinjo, H Motonaga, E Matayoshi, S Kudo, K Watanabe, M Hara, K Tabuchi, E Momotani, R Azuma
{"title":"Occurrence of bovine dermatophilosis in the southernmost islands of Japan.","authors":"E Kinjo, H Motonaga, E Matayoshi, S Kudo, K Watanabe, M Hara, K Tabuchi, E Momotani, R Azuma","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Such cutaneous symptoms as characteristic incrustation and alopecia were noticed in 25 calves of the indigenous Japanese Black breed grazing on subtropical islands in Japan over a period of April, 1978 to February, 1980. These islands were Ishigaki, Kuro, Yonaguni and Tarama belonging to the Sakishima Islands. Microbiological and pathological examination on three of these calves revealed that the calves were affected with dermatophilosis caused by Dermatophilus congolensis. The disease in these calves seemed to be the same as that reported previously in other countries, since it attacked young calves in a humid district with an abundant rainfall. It broke out first on Ishigaki Island and subsequently on the other islands in 3 years. Discussion was made on factors inducing these outbreaks.</p>","PeriodicalId":76197,"journal":{"name":"National Institute of Animal Health quarterly","volume":"21 4","pages":"163-74"},"PeriodicalIF":0.0,"publicationDate":"1981-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18351706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Morphology of sulfur granules produced by Pseudomonas aeruginosa in cows.","authors":"M Kubo, M Osada, S Konno","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A sulfur granule produced by Pseudomonas aeruginosa in three cows was studied light- and electron-microscopically. It consisted of clumps of basophilic bacteria and eosinophilic clubs. The clubs radiated from the periphery outward. The bacteria were stained dark red by both PAS and MacCallum-Goodpasture staining. Electron-microscopically, the sulfur granule consisted of electron-dense amorphous material and bacteria. The intact bacterium was about 0.45 micrometers in diameter and had an electron-dense cell wall 15 nm in width. Beneath the cell wall, moderately electron-dense fine granular material was present. The center of the bacterium was electron lucent. The club was electron-dense amorphous material. Degenerative bacteria and pilus-like structures were often seen in it. In one case, clusters of bacteriophages were present in and near the degenerative bacteria. Their head was hexagonal and 40 nm in diameter.</p>","PeriodicalId":76197,"journal":{"name":"National Institute of Animal Health quarterly","volume":"21 1","pages":"26-31"},"PeriodicalIF":0.0,"publicationDate":"1981-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17840001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M Takaya, K Akiyama, T Taniguchi, I Nonomura, T Horiuchi
{"title":"Fowl cholera found in imported myna birds (Eulabes intermedia).","authors":"M Takaya, K Akiyama, T Taniguchi, I Nonomura, T Horiuchi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Of 480 myna birds imported from Thailand in 1976, 54 birds died shortly after arrival in Japan. Bacteriological and pathological examination were carried out on seven of them. Pasteurella multocida was almost purely isolated from all the liver, heart, spleen and trachea examined. Somatic antigen of the isolants was O-5 of Namioka and Murata. Dissection revealed hyperemia of the trachea, edema of the lungs, increase of pleural fluid, and petechial or ecchymotic hemorrhages in the liver, heart and duodenum. Minute necrotic foci in the liver and bacterial clumps in the foci, pulmonary exudate and septa were found under the microscope. Severe pathogenicity of one of the isolated strains was proved by inoculation of 50-day-old broiler chickens with 10(3)-10(5) viable cells and by death within 18 hours, following the inoculation.</p>","PeriodicalId":76197,"journal":{"name":"National Institute of Animal Health quarterly","volume":"21 3","pages":"129-33"},"PeriodicalIF":0.0,"publicationDate":"1981-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18345031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Cellular fatty acid composition in staphylococci isolated from bovine milk.","authors":"H Sincoweay, E Miyagawa, T Kume","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Cellular fatty acid composition was examined in 61 strains of coagulase-negative staphylococci of bovine milk origin and 19 strains of Staphylococcus aureus and S. epidermidis serving as reference strains. The 61 strains had been divided into 9 species in accordance with the classification of Kloos & Schleifer. When S. aureus and the coagulase-negative staphylococci were examined, they contained iso-C15:0, anteiso-C15:0, C18:0, and C20:0 as main fatty acids. There was no marked difference in the cellular fatty acid composition between any two staphylococcal species. The percent cellular fatty acid composition of each species was regarded as one of the quantities of continuity and subjected to the analysis of variance by the unary configuration method. As a result, S. capitis, S. cohnii, S. epidermidis, and S. xylosus seemed to have a cellular fatty acid composition a little different from that of any other species. When similarity values among all the strains were calculated, they were 0.89 or more and made it possible to divide all the strains into two large groups at the similarity value of 0.90. In the one group the iso-C15:0 was a characteristic fatty acid. In the other group the anteiso-C15:0 was a characteristic fatty acid. To the first group belonged S. hyicus and S. lentus. To the second group belonged S. cohnii, S. epidermidis, S. haemolyticus, S. hominis, S. saprophyticus, and S. warneri. In the two groups were distributed S. aureus, S. capitis, S. simulans, and S. xylosus.</p>","PeriodicalId":76197,"journal":{"name":"National Institute of Animal Health quarterly","volume":"21 1","pages":"14-20"},"PeriodicalIF":0.0,"publicationDate":"1981-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18257072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Y Umemura, S Miyazaki, H Yamanaka, T Ohya, S Homma, M Oka, S Sato, T Nakahara
{"title":"Properties of gizzard erosion-inducing substance in fish meal.","authors":"Y Umemura, S Miyazaki, H Yamanaka, T Ohya, S Homma, M Oka, S Sato, T Nakahara","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Heated whole fish meal, produced from mackerel, was proved to induce gizzard erosion in broiler chicks by feeding for 6 days. The constituents of the water-soluble fraction of fish meal were suspected to be the cause of this lesion. Of them, histidine, histamine and nucleotides were examined. When added to milk casein and heated, histidine appeared to be the most toxic. It seemed, however, that histamine and IMP (inosine 5'-monophosphate) might be less toxic even when treated in the same way a histidine. Feeding of a large amount of histamine alone resulted in giving rise to a much disturbed profile of body weight gain, while a low incidence of gizzard erosion was observed in this case. Both heated fish meal and a heated mixture of casein and histidine were easily reduced in toxicity by acid hydrolysis. The latter was more sensitive to this treatment. On the contrary, papain had an action to diminish the toxicity of heated fish meal at all. Even after treated with papain, the heated casein-histidine mixture was still effective to induce gizzard erosion. These results might have come from different structural changes caused by heating of the two samples.</p>","PeriodicalId":76197,"journal":{"name":"National Institute of Animal Health quarterly","volume":"21 1","pages":"52-60"},"PeriodicalIF":0.0,"publicationDate":"1981-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18257074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Y Akaike, T Ogawa, T Miyachi, M Sugawara, Y Isayama, M Kashiwazaki
{"title":"Serum antibody against heat-labile enterotoxin of Escherichia coli in pigs.","authors":"Y Akaike, T Ogawa, T Miyachi, M Sugawara, Y Isayama, M Kashiwazaki","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":76197,"journal":{"name":"National Institute of Animal Health quarterly","volume":"21 1","pages":"61-2"},"PeriodicalIF":0.0,"publicationDate":"1981-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18066165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Micro method for performing titration and neutralization test of hog cholera virus using established porcine kidney cell strain.","authors":"H Komaniwa, A Fukusho, Y Shimizu","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Hog cholera (HC) virus and antibody against it were estimated by the END method with microplates and CPK porcine kidney cell strain. To establish the technique of this method, studies were made on such basic conditions of the method as the type of strain of Newcastle disease virus (NDV), the time of challenge with this virus, and the concentration of serum in culture fluid. There was little difference in the infective titer of HC virus estimated between the END method performed by the established technique and the same method by the conventional technique with test tubes and swine testicle (ST) cells. Besides, there was a high correlation between the neutralizing antibody titer measured by the one technique and that measured by the other. The coefficient of correlation was r = +0.948 in this case. From the experimental results mentioned above it was concluded that the END method by the micro-technique with CPK cells was simpler than and as reliable as the same method conducted by any conventional technique, and that it was a practicable one capable of testing many samples.</p>","PeriodicalId":76197,"journal":{"name":"National Institute of Animal Health quarterly","volume":"21 4","pages":"153-8"},"PeriodicalIF":0.0,"publicationDate":"1981-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18215973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K Nakamura, E Momotani, Y Yokomizo, H Yugi, S Shoya
{"title":"Pathological changes in sheep infected with Corynebacterium pseudotuberculosis.","authors":"K Nakamura, E Momotani, Y Yokomizo, H Yugi, S Shoya","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":76197,"journal":{"name":"National Institute of Animal Health quarterly","volume":"21 3","pages":"150-1"},"PeriodicalIF":0.0,"publicationDate":"1981-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18348284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T Taniguchi, S Yamaguchi, M Maeda, H Kawamura, T Horiuchi
{"title":"Pathological changes in laying hens inoculated with the JPA-1 strain of egg drop syndrome-1976 virus.","authors":"T Taniguchi, S Yamaguchi, M Maeda, H Kawamura, T Horiuchi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Rhode Island Red laying hens were inoculated with the JPA-1 strain of egg drop syndrome-1976 virus and examined pathologically 1, 3, 5, 7, 10, 14, 21, 28 and 80 days postinoculation (p.i.). Eggs with a discolored or soft shell or no shell were laid over a period from 10 to 24 days p.i. Egg production failed transiently in some affected birds after 13 days p.i. At autopsy, the uterus revealed remarkable edema and swelling of the mucosal folds together with the deposition of whitish exudate in the cavity 14 days p.i. Microscopically, intranuclear inclusion bodies were found in epithelial cells of the uterus, isthmus and vaginal gland region 10 and 14 days p.i. There were also severe degeneration and desquamation of the epithelial cells, atrophy of the uterine glands and remarkable infiltration of heterophils, lymphocytes and plasmacytes accompanied with extensive edema. Twenty-one days after inoculation, lymphoid follicles were formed in the mucosal folds of some part of the oviduct. Electron microscopic examination revealed various amounts of virus particles 68 approximately 80 nm in diameter mainly in the nuclei of the epithelial cells on the mucosa and in the exudate, and also in the cytoplasm of macrophages in the uterus.</p>","PeriodicalId":76197,"journal":{"name":"National Institute of Animal Health quarterly","volume":"21 2","pages":"83-93"},"PeriodicalIF":0.0,"publicationDate":"1981-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17343493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Pathogenicity of Getah virus for mice.","authors":"H Sentsui, Y Kono","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The Sakai strain of Getah virus isolated from a feverish horse was examined for pathogenicity for mice. Its pathogenicity depended on the age of the host and the route of inoculation. Intracerebral inoculation caused death to mice less than 9 days old. It induced retarded growth, insufficient hair growth, paralysis, and sporadic death in mice 11 days old, but no symptoms in mice 13 days old. The virus multiplied rapidly and extensively in every tested organ, particularly in the muscular tissue, of the suckling mouse. It was also observed that the virus was transmitted horizontally among the litter-mates and vertically from the dam to her offspring via milk.</p>","PeriodicalId":76197,"journal":{"name":"National Institute of Animal Health quarterly","volume":"21 1","pages":"7-13"},"PeriodicalIF":0.0,"publicationDate":"1981-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17326581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}