{"title":"Genetic relationships among 5 inbred strains established from common ancestor, dd mouse, as assessed by mandible analysis.","authors":"N Goto, K Miura, K Imamura, K Komeda","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>By multivariate analysis using mandible measurements genetic relationships were investigated among 5 inbred strains, DDD, DDK, DSD, DD/Tbr and DDY, derived from the common ancestor, dd mouse. (1) In canonical discriminant analysis (discriminant analysis with reduction of dimensionality), 3 DD strains, DDD, DSD, and DDY, were closer to one another than to any control strain on planes Z1-Z2, Z1-Z3, and Z2-Z3. Only the DDK strain was quite far from those 3 strains. (2) In the discriminant analysis, however, the 5 inbred strains could be classified correctly at the rate of 100%. They were genetically different from one another. (3) The causes for this genetical difference seemed to be the genetic heterogeneity of the ancestor dd mouse and the method of inbreeding which had been independent since the separation.</p>","PeriodicalId":76197,"journal":{"name":"National Institute of Animal Health quarterly","volume":"22 2","pages":"70-5"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18149017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Statistical analysis of results of a survey on antibody against swine influenza virus in pigs by single radial hemolysis and hemagglutination-inhibition tests.","authors":"T Ogawa, T Sugimura, Y Tanaka","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":76197,"journal":{"name":"National Institute of Animal Health quarterly","volume":"22 1","pages":"32-3"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18124180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Hematopathological changes in dead and moribund chicks induced by chicken anemia agent.","authors":"T Taniguchi, N Yuasa, M Maeda, T Horiuchi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Fifty day-old chicks were inoculated wit the Gifu-1 strain of chicken anemia agents. They began to show anemia and a drop in body weight 10 days postinoculation. Thirty-five of them died over a period of 12 to 23 days, mostly between 14 and 18 days postinoculation. Hematocrit value and red and white blood cell counts were markedly reduced in moribund birds. Polychromatic erythrocytes and granulocytes dropped in incidence in the peripheral blood accompanied with a fall in hematocrit value, and gained again with recovery from anemia. Macroscopically, dead and moribund birds revealed yellow bone marrow, marked atrophy of the thymus and bursa of Fabricius, discoloration and swelling of the liver, spleen and kidneys. In some cases, there were partially well-defined necrotic foci in the liver, round heart, fresh hemorrhages in the proventricular mucosa, erosion on the gizzard mucosa, and subcutaneous edema. Microscopically, marked hypoplasia or aplasia of hematopoietic cells in the bone marrow and severe depletion of lymphocytes in lymphoid organs, such as the thymus, bursa of Fabricius, spleen, and cecal tonsils, were commonly seen in affected birds. The changes described above suggested that the anemia induced by chicken anemia agent might closely be related to the bone marrow function.</p>","PeriodicalId":76197,"journal":{"name":"National Institute of Animal Health quarterly","volume":"22 2","pages":"61-9"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18149016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Outbreaks of type C botulism in waterfowl in Japan.","authors":"T Ono, R Azuma, T Kato, S Takeuchi, T Suto","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Four outbreaks of botulism in waterfowl were encountered over a five-year period of 1973 to 1977 in Japan. In all the outbreaks toxin was detected from all 12 sera, twenty-three of 24 gizzard contents from diseased or dead birds and one of three maggots. It was neutralized with Clostridium botulinum type C antitoxin serum, regardless of its origin. By using CO2 gas jet method, C. botulinum was isolated from four of 11 gizzards from diseased birds, five of 7 ones from dead birds, one of one maggot and one of one sludge sample, that is, eleven of 20 specimens in total. All 20 strains were identical with C. botulinum type C in biological properties. Most of the isolates showed a toxin titer ranging from 1,000 to 200,000 LD50 for mice. Four of them were identified as type C by mouse neutralization tests with antitoxin sera. The toxic suspensions of a strain 1-15 were administered orally to Chinese spot-billed ducks, which died when more than 200,000 LD50 mouse toxin was administered. Environmental conditions for occurrences of waterfowl botulism were discussed.</p>","PeriodicalId":76197,"journal":{"name":"National Institute of Animal Health quarterly","volume":"22 3","pages":"102-14"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17815200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Indirect immunoperoxidase technique for the assay of antibodies against Mycoplasma gallisepticum and M. synoviae in chicken serum.","authors":"Y Imada, I Nonomura, K Furuta","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The indirect immunoperoxidase technique (IIP) was applied to the assay of antibodies against Mycoplasma gallisepticum and M. synoviae in chicken serum by using colonies grown on the agar plate as antigen. the sensitivity and the specificity of IIP were evaluated by the use of sera from experimentally infected, filed, and SPF Chickens. As compared with tube agglutination and hemagglutination-inhibition tests, IIP was highly effective and specific for detecting antibodies against M. gallisepticum and M. synoviae.</p>","PeriodicalId":76197,"journal":{"name":"National Institute of Animal Health quarterly","volume":"22 1","pages":"16-22"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18090840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Detection of schizonts in a chicken recovered from experimental infection with Leucocytozoon caulleryi.","authors":"K Fujisaki, S Kitaoka, T Kamio, H Takamatsu","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":76197,"journal":{"name":"National Institute of Animal Health quarterly","volume":"22 3","pages":"144-5"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17871052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Stability of avian leukosis virus type-specific antigen in the cell after fixation in periodate-lysine-paraformaldehyde.","authors":"Y Mizuno, K Arai","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Stability of avian leukosis virus type-specific antigen in chicken embryo fibroblasts after fixation in periodate-lysine-paraformaldehyde was tested by the indirect immunoperoxidase method. Antigen that had been steeped in 0.015M Tris-HCl saline buffer solution containing 20% bovine serum and 30% glycerin was preserved at -20 degrees C, 4 degrees C and room temperature without loss of its antigenicity as long as 16 weeks. It was also stable against heat treatment at 56 degrees C, acid treatment and ethyl ether treatment.</p>","PeriodicalId":76197,"journal":{"name":"National Institute of Animal Health quarterly","volume":"22 1","pages":"34-5"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17241244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Detection of salmonella infection in herds by examination of slurry.","authors":"Y Sunaga, S Sato","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>An examination was carried out on the slurry system of the Animal Quarantine Service for 9 months to find the degree of contamination with Salmonella. Ready-made tampons were placed in the slurry for 5 to 7 days and submitted for detection of Salmonella. Tampons and samples of settled sludge were collected about two times a month at fixed sites along the slurry system. Salmonella organisms were recovered from 80 (62.5%) of 128 tampons, and also from 40 (34.8%) of 115 samples of settled sludge. The serovars isolated were S. cerro, S. newport, S. muenster, S. stanley, S. heidelberg, S. infantis, S. drypool, S. typhimurium, S. panama and S. arizonae (61:k:1, 5, 7). Seventy-one (98.6%) of 72 samples of slurry from which Salmonella organisms had been isolated by the tampon method contained less than 100 organisms as most probable number (MPN) per 100 ml. Thirty-four (91.9%) of 37 samples of settled sludge contained these organisms at the rate of less than 100/100 ml. Further examination was carried out on the slurry of pits and gutters beside animal houses and kennel during a period of two months when a large number of animals were put into quarantine. As a result, the states of Salmonella contamination could be clarified in each animal house. The tampon method is simple, inexpensive and reliable for the isolation of Salmonella organisms from the slurry which contains less than 1 organism 100 ml, as compared with such method of examination for fecal samples from many individual animals and a large quantity of slurry.</p>","PeriodicalId":76197,"journal":{"name":"National Institute of Animal Health quarterly","volume":"22 2","pages":"39-44"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17807898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of temperature on the survival of Haemophilus parasuis in physiological saline.","authors":"T Morozumi, T Hiramune","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The survival of Haemophilus parasuis in physiological saline and in sodium phosphate buffered saline (PBS) at various temperatures was investigated and compared with that of H. pleuropneumoniae, Pasteurella multocida, and Escherichia coli. When placed in physiological saline, H. parasuis became undetectable at 42 degrees C within one hour, at 37 degrees C within 2 hours, and at 25 degrees C within 8 hours, but decreased slightly in amount at 5 degrees C for 8 hours. It showed the same tendency when placed in PBS. In the same condition, H. plueropneumoniae decreased more slowly in amount than H. parasuis, P. multocida was much more stable than H. parasuis, and E. coli hardly decreased even at 42 degrees C for 8 hours.</p>","PeriodicalId":76197,"journal":{"name":"National Institute of Animal Health quarterly","volume":"22 2","pages":"90-1"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17807899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Syncytia infectivity of assay of bovine leukemia virus.","authors":"S Itohara, I Takatori","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Bovine embryonic spleen cell cultures were examined to find several factors influencing the specificity, sensitivity and reproducibility of the syncytia infectivity assay of bovine leukemia virus (BLV). The highest sensitivity of the assay were observed when cell sheets of 30 to 50% confluence were inoculated with a stock of BLV, and when cells containing 4 or more nuclei were counted as syncytial cells. Treatment of the cell sheets with a diethylamino-ethyl-dextran solution (25 micrograms/ml) prior to BLV inoculation was found to be essential for the optimal induction of syncytia. Low-passage cultures were found to be more susceptible to the induction of syncytia by BLV than high-passage cultures. Cell-free BLV preparations decreased in syncytia-inducing ability to some extent by the first cycle of freezing (at -70 degrees C) and thawing. No further decrease, however, was caused by repeated cycles of freezing and thawing or by prolonged incuvation at -80 degrees C. The syncytia-inducing activity of BLV was inhibited by all the BLV-precipitating antibody-positive sera originated from both cases of the adult form of bovine leukosis and cases of persistent lymphocytosis. It was not inhibited by the sera of 16 of 17 cattle apparently healthy and negative for BLV-precipitating antibody. These results indicate that the syncytia infectivity assay and syncytia inhibition test are specific for BLV.</p>","PeriodicalId":76197,"journal":{"name":"National Institute of Animal Health quarterly","volume":"22 4","pages":"147-53"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17373215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}