Stability of avian leukosis virus type-specific antigen in the cell after fixation in periodate-lysine-paraformaldehyde.

Abstract

Stability of avian leukosis virus type-specific antigen in chicken embryo fibroblasts after fixation in periodate-lysine-paraformaldehyde was tested by the indirect immunoperoxidase method. Antigen that had been steeped in 0.015M Tris-HCl saline buffer solution containing 20% bovine serum and 30% glycerin was preserved at -20 degrees C, 4 degrees C and room temperature without loss of its antigenicity as long as 16 weeks. It was also stable against heat treatment at 56 degrees C, acid treatment and ethyl ether treatment.