C J Slager, J H Reiber, G T Meester, J C Schuurbiers
{"title":"Automated feature extraction from cineangiograms. Experience with routinely used hardwired system Contouromat.","authors":"C J Slager, J H Reiber, G T Meester, J C Schuurbiers","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":76159,"journal":{"name":"Microscopica acta. Supplement","volume":" 3","pages":"231-3"},"PeriodicalIF":0.0,"publicationDate":"1979-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11509842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Correlation of morphometric parameters with enzyme-activities in cases of liver diseases with fibrosis (author's transl)].","authors":"N Dahrenmöller, A Roessner, G Pott, K M Müller","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>50 liver specimens with fibrotic liver disease were taken from current autopsy material and, after preparation, subjected to automatized image analysis by QUANTIMET 720 for quantitation of the connective tissues and their cellularity. In cell-free supernatants of liver tissue homogenates we determined the activities of N-acetyl-beta-glucosaminidase (beta-NAG, E.C. 3.2.1.30), a lysosomal glycosidase that is involved in proteoglycane metabolism of connective tissue. Beta-NAG-isoenzymes were demonstrated by electrofocusing in thin layers of polyamide gel. The results showed statistically significant correlations between beta-NAG activity and morphometrically assessed quantities of connective tissue, and of mesenchymal cells found in the latter or in the whole specimen, respectively. Demonstration of tissue or serum levels of beta-NAG or its isoenzymes provides a readily practicable method for assessing mesenchymal activities of the liver.</p>","PeriodicalId":76159,"journal":{"name":"Microscopica acta. Supplement","volume":" 3","pages":"191-6"},"PeriodicalIF":0.0,"publicationDate":"1979-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11509839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Application of x-ray microanalysis to studies on distribution of diffusible ions in skeletal muscle tissue.","authors":"K Zierold, D Schäfer, J Gullasch","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":76159,"journal":{"name":"Microscopica acta. Supplement","volume":" 2","pages":"92-101"},"PeriodicalIF":0.0,"publicationDate":"1978-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11507034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"X-ray microanalysis of frozen hydrated tissue sections as a physiological tool.","authors":"A J Saubermann","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Frozen hydrated tissue sections can be used as a physiological tool. The technique is a difficult one primarily because of the numerous possibilities for artifcatual interference. However, these problems are becoming better understood and the information which can be obtained through the use of these techniques is extremely promising.</p>","PeriodicalId":76159,"journal":{"name":"Microscopica acta. Supplement","volume":" 2","pages":"130-41"},"PeriodicalIF":0.0,"publicationDate":"1978-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11507262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Application of electron probe x-ray microanalysis to the detection of metal pollutants in freshwater zooplankton.","authors":"C Chassard-Bouchard, G Balvay","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":76159,"journal":{"name":"Microscopica acta. Supplement","volume":" 2","pages":"185-92"},"PeriodicalIF":0.0,"publicationDate":"1978-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11507266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The nuclear microprobe, actual state of instrumentation and possible biomedical implications.","authors":"T B Pierce","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":76159,"journal":{"name":"Microscopica acta. Supplement","volume":" 2","pages":"318-30"},"PeriodicalIF":0.0,"publicationDate":"1978-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11507276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A BASIC-language program for dedicated energy-dispersive microanalysis of thin samples in TEM or STEM.","authors":"J C Russ","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The program QTHIN combines those methods for processing energy-dispersive X-ray spectra and computing concentrations best suited to thin section microanalysis in TEM or STEM. The program is in BASIC, and so can be modified (for instance for unusual geometries, other matrices such as oxides, etc.). It is suitable for routine use on complex samples with peak overlaps, and for \"not-so-thin\" samples in which absorption is not negligible.</p>","PeriodicalId":76159,"journal":{"name":"Microscopica acta. Supplement","volume":" 2","pages":"217-27"},"PeriodicalIF":0.0,"publicationDate":"1978-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11507268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
W Bröcker, G Hauck, R Blaschke, H Schröer, G Pfefferkorn
{"title":"Cathodoluminescence technique combined with blue light fluorescence microscopy for the investigation of the transport of molecules through the extravascular space of mammalians.","authors":"W Bröcker, G Hauck, R Blaschke, H Schröer, G Pfefferkorn","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The mechanisms of transport of molecules across the extravascular spaces represent an open problem. Vital microscopic results with marked proteins using the blue light luminescence technique indicate a movement of fluid along the connective tissue fibres. Furthermore, the findings suggest that the elastic fibres play an important role in this movement. It may also be possible to elucidate the extravascular pathway for molecules by means of the cathodoluminescence technique in the scanning electron microscope. The investigations were performed using the mesentery of mammalians anaesthetized by Nembutal. The biological material for cathodoluminescence applications was selected from animals without injection of luminescent dye as well as with animals following intravenous injection of marked proteins. Two techniques, critical point drying and freeze drying, were used in the preparation. Stained and unstained tissues were investigated in the scanning electron microscope with a special cathodoluminescence detector system. Simultaneously observations of the tissue surfaces were performed by means of the secondary electron or backscattered electron mode. The results obtained agree with the findings observed by the blue light fluorescence microscopy and confirm the concept of an extravascular fluid pathway along the elastic fibres in the tissue.</p>","PeriodicalId":76159,"journal":{"name":"Microscopica acta. Supplement","volume":" 2","pages":"260-70"},"PeriodicalIF":0.0,"publicationDate":"1978-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11507272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Chemical analysis of biological materials with the laser probe mass-spectrograph.","authors":"J F Eloy","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":76159,"journal":{"name":"Microscopica acta. Supplement","volume":" 2","pages":"307-17"},"PeriodicalIF":0.0,"publicationDate":"1978-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11507275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}