Microscopica acta. Supplement最新文献_第10页

Metaphase location and chromosome identification by means of the Quantimet 720D. A semi-automatic approach. 利用Quantimet 720D进行中期定位和染色体鉴定。半自动的方法。

Microscopica acta. Supplement Pub Date : 1977-01-01

H F de France, H T van den Berg

引用次数: 0

Procedure of sampling in the layered cortex. 层状皮层取样程序。

Microscopica acta. Supplement Pub Date : 1977-01-01

H Haug

引用次数: 0

[Morphometry of breast tissue (author's transl)]. [乳腺组织形态测量学(作者译)]。

Microscopica acta. Supplement Pub Date : 1977-01-01

H Nienhaus, M Brenner

引用次数: 0

[Selective contrast enhancement of microscopical specimen by optical procedures for automatized quantitative image analysis (author's transl)]. [通过自动定量图像分析的光学程序对显微标本进行选择性对比度增强[作者简介]。

Microscopica acta. Supplement Pub Date : 1977-01-01

J Bereiter-Hahn

{"title":"[Selective contrast enhancement of microscopical specimen by optical procedures for automatized quantitative image analysis (author's transl)].","authors":"J Bereiter-Hahn","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>One of the most important problems in automatic image analysis is the discrimination of features by, a certain range of grey levels. To obtain as many as possible different grey levels sufficient contrast is achieved mainly by specific staining. Alternative and supplementary methods are some micrscopical methods, not routinely used. Interference microscopy, interference reflexion microscopy and microfluorimetry are discussed in detail. These optical procedures enhance the contrast of specimen specifically without the necessity for the application of specimen specifically without the necessity for the application of sophisticated staining methods. In interference contrast, tissues can be separately detected by grey level discrimination due to varying concentration of dry mass; this is shown for a cornifying part of fish skin (breeding tubercle of Rutilus rutilus L.). Furthermore, very small amounts of dry matter can be determined with high precision, as demonstrated for a single tissue culture cell (XTH-cell). Automatisation of image analysis provides a unique opportunity for routine application of interferometric measurements. The principles of the procedures are outlined. By interference reflexion microscopy cellular attachment areas to a glass surface are visualized, providing a powerful tool in cellular diagnosis based on grey level discrimination (darkest parts correspond to zones of closest contact to the substratum). A fast migrating lymphocyte and stationary endothelial cells have been chosen for demonstration. Various histochemical problems can be solved elegantly by fluorescence methods, e.g. mitochondria in living cells are specifically stained by a fluorochrome (DASPMI) and the distribution of fluorescence intensity can be followed within the mitochondrial population of a cell. Fluorescence was recorded from fotographic negatives taken with a fluorescence microscope. Additionally a short comment is given on the application of polarisation microscopy for feature detection.</p>","PeriodicalId":76159,"journal":{"name":"Microscopica acta. Supplement","volume":" 1","pages":"165-80"},"PeriodicalIF":0.0,"publicationDate":"1977-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11600006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantification of chromatin structure and DNA contents in nuclei of cultured cells by texture-analysis-system. 质构分析系统定量培养细胞细胞核染色质结构及DNA含量。

Microscopica acta. Supplement Pub Date : 1977-01-01

K Hinrichsen, P Bachmann

引用次数: 0

[Selective and high-contrast staining in nervous tissue (author's transl)]. [神经组织的选择性和高对比染色(作者图)]。

Microscopica acta. Supplement Pub Date : 1977-01-01

S Eins, F Gallyas

引用次数: 0

Reconstruction of (types of) lymphocytes from serial sections. 从连续切片中重建淋巴细胞(类型)。

Microscopica acta. Supplement Pub Date : 1977-01-01

C L Alons