Cathodoluminescence technique combined with blue light fluorescence microscopy for the investigation of the transport of molecules through the extravascular space of mammalians.

Microscopica acta. Supplement Pub Date : 1978-01-01
W Bröcker, G Hauck, R Blaschke, H Schröer, G Pfefferkorn
{"title":"Cathodoluminescence technique combined with blue light fluorescence microscopy for the investigation of the transport of molecules through the extravascular space of mammalians.","authors":"W Bröcker,&nbsp;G Hauck,&nbsp;R Blaschke,&nbsp;H Schröer,&nbsp;G Pfefferkorn","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>The mechanisms of transport of molecules across the extravascular spaces represent an open problem. Vital microscopic results with marked proteins using the blue light luminescence technique indicate a movement of fluid along the connective tissue fibres. Furthermore, the findings suggest that the elastic fibres play an important role in this movement. It may also be possible to elucidate the extravascular pathway for molecules by means of the cathodoluminescence technique in the scanning electron microscope. The investigations were performed using the mesentery of mammalians anaesthetized by Nembutal. The biological material for cathodoluminescence applications was selected from animals without injection of luminescent dye as well as with animals following intravenous injection of marked proteins. Two techniques, critical point drying and freeze drying, were used in the preparation. Stained and unstained tissues were investigated in the scanning electron microscope with a special cathodoluminescence detector system. Simultaneously observations of the tissue surfaces were performed by means of the secondary electron or backscattered electron mode. The results obtained agree with the findings observed by the blue light fluorescence microscopy and confirm the concept of an extravascular fluid pathway along the elastic fibres in the tissue.</p>","PeriodicalId":76159,"journal":{"name":"Microscopica acta. Supplement","volume":" 2","pages":"260-70"},"PeriodicalIF":0.0000,"publicationDate":"1978-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microscopica acta. Supplement","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

The mechanisms of transport of molecules across the extravascular spaces represent an open problem. Vital microscopic results with marked proteins using the blue light luminescence technique indicate a movement of fluid along the connective tissue fibres. Furthermore, the findings suggest that the elastic fibres play an important role in this movement. It may also be possible to elucidate the extravascular pathway for molecules by means of the cathodoluminescence technique in the scanning electron microscope. The investigations were performed using the mesentery of mammalians anaesthetized by Nembutal. The biological material for cathodoluminescence applications was selected from animals without injection of luminescent dye as well as with animals following intravenous injection of marked proteins. Two techniques, critical point drying and freeze drying, were used in the preparation. Stained and unstained tissues were investigated in the scanning electron microscope with a special cathodoluminescence detector system. Simultaneously observations of the tissue surfaces were performed by means of the secondary electron or backscattered electron mode. The results obtained agree with the findings observed by the blue light fluorescence microscopy and confirm the concept of an extravascular fluid pathway along the elastic fibres in the tissue.

阴极发光技术结合蓝光荧光显微镜研究哺乳动物血管外空间的分子转运。
分子在血管外空间的运输机制是一个悬而未决的问题。使用蓝光发光技术对标记蛋白质的重要显微结果表明,液体沿着结缔组织纤维运动。此外,研究结果表明,弹性纤维在这种运动中起着重要作用。利用扫描电子显微镜的阴极发光技术也可以阐明分子的血管外通路。研究是在用Nembutal麻醉的哺乳动物的肠系膜上进行的。用于阴极发光应用的生物材料分别取自未注射荧光染料的动物和静脉注射标记蛋白的动物。采用临界点干燥和冷冻干燥两种技术制备。用专用的阴极发光检测系统在扫描电镜下观察染色组织和未染色组织。同时,通过二次电子或背散射电子模式对组织表面进行了观察。所获得的结果与蓝光荧光显微镜观察到的结果一致,并证实了沿组织中弹性纤维的血管外液体通路的概念。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信