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[Investigations on the conditions of scanning on cell images by means of a microscope-TV-system (author's transl)]. [用显微镜-电视系统扫描细胞图像条件的研究[作者译]。
Microscopica acta Pub Date : 1981-09-01
H Harms, S Boseck, H M Aus, V Lenz
{"title":"[Investigations on the conditions of scanning on cell images by means of a microscope-TV-system (author's transl)].","authors":"H Harms,&nbsp;S Boseck,&nbsp;H M Aus,&nbsp;V Lenz","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>One common method for determining the spatial resolution of a TV-microscope scanning system is to measure a sharp edge, differentiate the digitized response and calculate the Modulation Transfer Function (MTF) using the Fourier transformation. The MTF provides a measure of the information content of a digitized picture as well as the resolving power of the whole system. Knowledge of this measure is prerequisite for understanding the capabilities and limitations of computer aided techniques for analyzing cellular structures as observed in the light microscope. The information content of a digitized image depends on the MTF of the whole measurement system. Results from this study show that the information content increases almost linearly up to a sampling rate of approximately 20 pixel/micrometer and saturates at 30--35 pixel/micrometer. The MTF and the signal/noise ratio is effected by averaged multiscans. The aliasing error is presented as a function of the sampling rate for the described system. The need for this resolving power of the cell image analysis system is demonstrated by an example in the haematology. Small granules with a diameter of 0.2 to 1.0 micrometer must be registered with a high sampling rate up to 30 pixel/micrometer. The analysis of the small granules is basic for automated detection and differentiation of many leukemic conditions.</p>","PeriodicalId":76158,"journal":{"name":"Microscopica acta","volume":"85 1","pages":"69-82"},"PeriodicalIF":0.0,"publicationDate":"1981-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18075791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[ACTH measurement in the hypophysis after adrenalectomy and adrenal cortex hormone therapy by means of microphotometry and radioimmunoassay method (author's transl)]. [用显微光度法和放射免疫法测定肾上腺切除术和肾上腺皮质激素治疗后垂体ACTH的变化[作者简介]。
Microscopica acta Pub Date : 1981-07-01
F Lange
{"title":"[ACTH measurement in the hypophysis after adrenalectomy and adrenal cortex hormone therapy by means of microphotometry and radioimmunoassay method (author's transl)].","authors":"F Lange","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In experiments after adrenalectomy and adrenal cortex hormone therapy the quantity of ACTH was ascertained in the hypophysis of cats by methods of microphotometry and radioimmunoassay. The deviation of increased or decreased amounts was studied in acute as well as in chronic physiological conditions of the cats. The different methods to measure the quantities are equally identifying the physiological conditions.</p>","PeriodicalId":76158,"journal":{"name":"Microscopica acta","volume":"84 4","pages":"329-37"},"PeriodicalIF":0.0,"publicationDate":"1981-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17329670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Influence of UV rays on Feulgen-type staining with azure A-SO2 prepared with normal hydrochloric acid and sodium thiosulphate. 紫外光对用普通盐酸和硫代硫酸钠制备的蓝色A-SO2 feulgen型染色的影响。
Microscopica acta Pub Date : 1981-07-01
M K Dutt
{"title":"Influence of UV rays on Feulgen-type staining with azure A-SO2 prepared with normal hydrochloric acid and sodium thiosulphate.","authors":"M K Dutt","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>This communication presents a new method for the preparation of azure A-SO2 for use in Feulgen procedure. The salient feature of this method lies in the fact that azure A-SO2 can be decolourised with normal hydrochloric acid and sodium thiosulphate. The pH of this dye reagent is 2.3 and it is of water colour after filtration. The pH of this dye-reagent is raised to 4.0 with an aqueous solution of sodium hydroxide. Nuclear colouration with this newly developed dye-reagent on acid-hydrolysed DNA of tissue sections becomes fairly satisfactory under the usual laboratory conditions. Staining with this dye-reagent under exposure to UV ray is, however, vastly improved within 5 minutes as compared with the control. Stained sections do withstand treatment in SO2 water without exhibiting any leaching of the dye from the nuclei. Possible mode of action of UV rays in increasing the intensity of staining as well as the speed of reaction has been suggested.</p>","PeriodicalId":76158,"journal":{"name":"Microscopica acta","volume":"84 4","pages":"339-44"},"PeriodicalIF":0.0,"publicationDate":"1981-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17233753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Two phases of the bone mineral as revealed by the high resolution scanning electron microscope on ion-etched bone surfaces and as seen on surfaces untreated and chemically etched. 高分辨率扫描电子显微镜在离子蚀刻的骨表面和未经处理和化学蚀刻的表面上显示的骨矿物的两种相。
Microscopica acta Pub Date : 1981-07-01
Z A Rális, I G Turner
{"title":"Two phases of the bone mineral as revealed by the high resolution scanning electron microscope on ion-etched bone surfaces and as seen on surfaces untreated and chemically etched.","authors":"Z A Rális,&nbsp;I G Turner","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Recently reported uses of the technique of high resolution scanning microscopy of polished and Argon ion-etched bone surfaces have revealed that, at the ultrastructural level, the bone mineral is spatially arranged in a network of twisted, closely packed segments containing globular and cylindrical components. The ion-etching technique, which preferentially removes organic and less dense material from the bone surface, has been subsequently used by the present authors for detailed screening of a quantity of human cortical and trabecular bone of different age and maturity during which it has been found that apart from this \"structured bone' containing twisted segments, the mineral is also organised in another regular form, the \"lining bone', which has the appearance of solid, smooth and dense slabs or sheets lining active bone surfaces and bone cell lacunae and canaliculi. In the present study, in order to exclude the possibility that these two newly described phases of the bone mineral microskeleton are results of an etching artifact, their SEM appearance in 14 Argon ion-etched human bone specimens from individuals aged 11 post-natal days to 79 years was compared in various compartments to that seen on surfaces which were untreated, just polished or etched by hot NaOCl. The results have shown that both the structured and lining bone are genuine features since although the best results for their clear and reliable distinction were achieved by the ion-etching technique, these images could also be recognised on bone surfaces which were untreated or treated in a different way.</p>","PeriodicalId":76158,"journal":{"name":"Microscopica acta","volume":"84 4","pages":"385-400"},"PeriodicalIF":0.0,"publicationDate":"1981-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18069307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A study of the histological basis of textural analysis. 对结构分析的组织学基础的研究。
Microscopica acta Pub Date : 1981-07-01
W Slidders, A J Robertson, A E Livesey, I A Cree, R A Brown, J S Beck
{"title":"A study of the histological basis of textural analysis.","authors":"W Slidders,&nbsp;A J Robertson,&nbsp;A E Livesey,&nbsp;I A Cree,&nbsp;R A Brown,&nbsp;J S Beck","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In an attempt to quantitate histological structure by textural analysis, the characters of a stained microscopical field were recorded as a frequency polygon of the relative areas defined by successive increments of optical density: this is known as the optical density/area (OD/A) profile. In a study of lymph-nodes in Hodgkin's disease and of carcinoma of the female breast, each stained tissue section was found to have an OD/A profile of characteristic shape: moreover, in each of the lesions studied it was shown that the shape of the profile could be replicated by summing the profiles of each constituent cell type and intercellular matrix in proportions determined by differential cell counts and direct measurement of the degree of cell packing in the tissue. In an investigation of the influence of specimen preparation on OD/A profile, it was shown that density of staining and section thickness were important variables. Using the density of staining of tissue lymphocytes as an internal standard, a simple method was developed of compensating for differences in the density of Mayer's haemalum staining greater than those met with in practice. Attempts to compensate for intentionally large differences in section thickness were much less successful, but this was considered to be relatively unimportant since, in practice, the variation in thickness of sections cut on a modern microtome by a competent microtomist had been shown to be slight and to have a negligible effect on the OD/A profile.</p>","PeriodicalId":76158,"journal":{"name":"Microscopica acta","volume":"84 4","pages":"361-78"},"PeriodicalIF":0.0,"publicationDate":"1981-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17233755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Fate of the Escherichia coli capsule during embedding procedures for electron microscopical studies. 大肠杆菌胶囊在电子显微镜下包埋过程中的命运。
Microscopica acta Pub Date : 1981-07-01
E N Schmid
{"title":"Fate of the Escherichia coli capsule during embedding procedures for electron microscopical studies.","authors":"E N Schmid","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The effect of the preparation steps of the TEM embedding procedure on the preservation of the Escherichia coli capsule was studied light microscopically. Electron microscopical micrographs did not distinctly reveal capsules whereas simultaneously performed India ink assays clearly revealed predominant capsules both on viable and on chemically fixed bacteria. It is clearly shown that dehydration does not cause extraction or collapse of capsular material to an extent that would explain the invisibility of the capsule on the electron microscopical level, as has hitherto been supposed.</p>","PeriodicalId":76158,"journal":{"name":"Microscopica acta","volume":"84 4","pages":"353-60"},"PeriodicalIF":0.0,"publicationDate":"1981-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17233754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Preparation of a new red dye from Janus black and its use in the staining of DNA-aldehyde molecules. 一种新的Janus black红色染料的制备及其在dna -醛分子染色中的应用。
Microscopica acta Pub Date : 1981-07-01
M K Dutt
{"title":"Preparation of a new red dye from Janus black and its use in the staining of DNA-aldehyde molecules.","authors":"M K Dutt","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>This communication presents a method for the preparation of a new red dye from an aqueous solution of Janus black by adding NHC1 and sodium thiosulphate to it. This new red dye when used on acid-hydrolysed tissue sections reveals the presence of red nuclei when sections after staining are dried between folds of filter paper, differentiated in n-butanol, cleared in xylene and mounted. Similarly stained sections when treated with SO2 water show partial leaching of the dye from the nuclei. Tissue sections when treated with cold concentrated phosphoric acid for 20 min and then stained with an aqueous solution of Janus black reveal the presence of orange-red nuclei. The new red dye obtained from Janus black does not respond to treatment under UV rays. The in vitro absorption data of the red dye indicate peaks at 210, 270 and 545 nm. The in situ absorption spectra of nuclei stained with the new red dye following Feulgen procedure reveal the peak of maximum absorption at 560 nm and those of nuclei treated with cold concentrated phosphoric acid and then stained with this red dye reveal peak at 530--540 nm. Some relevant points raised out of this investigation have been discussed.</p>","PeriodicalId":76158,"journal":{"name":"Microscopica acta","volume":"84 4","pages":"379-84"},"PeriodicalIF":0.0,"publicationDate":"1981-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17233756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Micro-injection by thermal expansion. 热膨胀微注射。
Microscopica acta Pub Date : 1981-05-01
M Zalokar
{"title":"Micro-injection by thermal expansion.","authors":"M Zalokar","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A micropipette (diameter 5 to 20 micron) sealed near the orifice to provide a small closed reservoir is described. The reservoir is filled with oil and can be heated with a tiny electric resistance wire loop. Thermal expansion and contraction of the oil in the reservoir allows liquid to be expelled or aspirated. The flow of the liquid can be controlled accurately by varying electric current. Detailed instructions are given for fabricating the micropipette and the heating assembly. A plan for a handy micropipette puller is given. The technique has proved to be valuable in nuclear transplantation and injection of fluid volumes between 1 and 100 picoliters into Drosophila eggs.</p>","PeriodicalId":76158,"journal":{"name":"Microscopica acta","volume":"84 3","pages":"231-7"},"PeriodicalIF":0.0,"publicationDate":"1981-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18210716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Propionic acid-metabisulphite-Schiff reagent for quick Feulgen staining. 丙酸-代谢亚硫酸盐-希夫试剂快速Feulgen染色。
Microscopica acta Pub Date : 1981-05-01
M K Dutt
{"title":"Propionic acid-metabisulphite-Schiff reagent for quick Feulgen staining.","authors":"M K Dutt","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>This communication presents a method for the preparation of Schiff reagent in which N hydrochloric acid has been replaced by a low concentration of propionic acid. The result of using such a Schiff reagent indicates that the dye-reagent thus prepared is extrafast in action on acid-hydrolysed mammalian tissue sections. The intensity of nuclear colouration is also increased considerably, since the pH of the dye-reagent is 6.0. It is, therefore advocated that this newly developed Schiff reagent be used at 5 degrees C.</p>","PeriodicalId":76158,"journal":{"name":"Microscopica acta","volume":"84 3","pages":"245-8"},"PeriodicalIF":0.0,"publicationDate":"1981-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17231679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Observations on the chromatin staining and fluorescence by safranine O. 藏红花O染色及荧光观察。
Microscopica acta Pub Date : 1981-05-01
R H Espelosin, P Eraso, J C Stockert
{"title":"Observations on the chromatin staining and fluorescence by safranine O.","authors":"R H Espelosin,&nbsp;P Eraso,&nbsp;J C Stockert","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>After treatment with safranine O at low concentrations (10(-6), 10(-5) M), the chromatin of chicken erythrocytes shows an intense red fluorescence, which is reduced or practically abolished when nuclei become stained by using the dye at concentrations higher than 10(-4) M. Both the fluorescence and staining reactions are dependent on the DNA content of chromatin. Safranine fluorescence of nuclei is not reduced in presence of high salt concentration, while the previous treatment with thiazine dyes diminishes the fluorescence intensity. The possibility that chromatin fluorescence depends on the intercalative binding of safranine is suggested.</p>","PeriodicalId":76158,"journal":{"name":"Microscopica acta","volume":"84 3","pages":"255-9"},"PeriodicalIF":0.0,"publicationDate":"1981-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17231681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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