丙酸-代谢亚硫酸盐-希夫试剂快速Feulgen染色。

Microscopica acta Pub Date : 1981-05-01

M K Dutt

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摘要

本文介绍了一种用低浓度丙酸代替盐酸制备希夫试剂的方法。使用这种希夫试剂的结果表明，这样制备的染料试剂对酸水解的哺乳动物组织切片具有超快的作用。核着色的强度也大大增加，因为染料试剂的pH值为6.0。因此，建议在5℃下使用这种新研制的希夫试剂。

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Propionic acid-metabisulphite-Schiff reagent for quick Feulgen staining.

This communication presents a method for the preparation of Schiff reagent in which N hydrochloric acid has been replaced by a low concentration of propionic acid. The result of using such a Schiff reagent indicates that the dye-reagent thus prepared is extrafast in action on acid-hydrolysed mammalian tissue sections. The intensity of nuclear colouration is also increased considerably, since the pH of the dye-reagent is 6.0. It is, therefore advocated that this newly developed Schiff reagent be used at 5 degrees C.

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Microscopica acta

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