发布求助
文献互助 智能选刊 最新文献

Microscopica acta最新文献

筛选
英文 中文
An established routine method for differential staining of epoxy-embedded tissue sections. 建立了环氧树脂包埋组织切片鉴别染色的常规方法。
Microscopica acta Pub Date : 1980-05-01
H E Schroeder, K Rossinsky, W Müller
{"title":"An established routine method for differential staining of epoxy-embedded tissue sections.","authors":"H E Schroeder,&nbsp;K Rossinsky,&nbsp;W Müller","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A method is described for staining semithin sections of epoxy-embedded cells and tissues. PAS-positive structures are specifically demonstrated by a red staining providing a vivid contrast to the other tissue components differentially stained in shades of blue, using methylene blue/azure II. Typical staining conditions for 1 to 2 micron thick Epon sections include 30 min oxidation with 5% periodic acid at 50 degrees C, 30 min incubation with Shiff's reagent at 50 degrees C, 20 min counter-staining with 0.5% methylene blue/0.5% azure II in 0.5% aqueous borax solution at room temperature. For 10 years, this method has provided excellent differential staining with a variety of tissues. Stained sections showed no signs of fading during this period of time. Therefore, this procedure is recommended as a simple method of staining semithin sections both for tissue orientation in electron microscopy and for brillant representation of cells and tissues, required for microphotography in color or black-and-white.</p>","PeriodicalId":76158,"journal":{"name":"Microscopica acta","volume":"83 2","pages":"111-6"},"PeriodicalIF":0.0,"publicationDate":"1980-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17221998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
In situ demonstration of DNA with Schiff-type dyes prepared with meta-phosphoric or tartaric acid. 用偏磷酸或酒石酸制备的希夫型染料进行DNA原位演示。
Microscopica acta Pub Date : 1980-05-01
M K Dutt
{"title":"In situ demonstration of DNA with Schiff-type dyes prepared with meta-phosphoric or tartaric acid.","authors":"M K Dutt","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A new method for the preparation of azure A-SO2 and safranine-SO2 For use in Feulgen procedure has been described herein. The method involves the use of m-phosphoric acid or tartaric acid in place of N HCl in the preparation of these eye-reagents which exhibit enhanced pH producing increased staining intensity of the nuclei as compared with those of the controls, prepared with N HCl. Possible explanation for the increased staining intensity as well as the reason for the shorter shelf-life of these eye-reagents have been offered.</p>","PeriodicalId":76158,"journal":{"name":"Microscopica acta","volume":"83 2","pages":"97-101"},"PeriodicalIF":0.0,"publicationDate":"1980-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17222000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Modified histotechnical procedure for tissue evaluation of microvascular prostheses (author's transl)]. [微血管假体组织评估的改进组织技术程序(作者译)]。
Microscopica acta Pub Date : 1980-05-01
C Tizian
{"title":"[Modified histotechnical procedure for tissue evaluation of microvascular prostheses (author's transl)].","authors":"C Tizian","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Porous silicone microvascular prostheses with a 1 mm inside diameter were inserted in the infrarenal part of the abdominal aorta of 35 rats. By modifying the usual histotechnical procedures like using paraffin with high consistence, embedding the specimen under vacuum and icing the sectional plane it was mde possible to produce perfect histological sections which guaranteed an exact histological evaluation of the implanted microvascular prostheses.</p>","PeriodicalId":76158,"journal":{"name":"Microscopica acta","volume":"83 2","pages":"123-6"},"PeriodicalIF":0.0,"publicationDate":"1980-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18041128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Stevenel's Blue, an excellent stain for optical microscopical study of plastic embedded tissues. 斯蒂夫蓝，一种很好的染色剂，用于塑料嵌套组织的光学显微研究。
Microscopica acta Pub Date : 1980-05-01
M del Cerro, J Cogen, C del Cerro
{"title":"Stevenel's Blue, an excellent stain for optical microscopical study of plastic embedded tissues.","authors":"M del Cerro,&nbsp;J Cogen,&nbsp;C del Cerro","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Stevenel's Blue is a reliable, rapid, and clean, one-step polychromatic stain for 1 micron thick epoxy sections. The staining solution, originally used by L. Stevenel (1918) to stain human parasites, is made by adding diluted potassium permanganate (2%) to an aqueous solution of the methylene blue (1.3%) and redissolving the precipitate thoroughly, by boiling in water bath and filtering. Staining is carried out in a Coplin jar at 60 degrees C for approximately 10 minutes for tissues embedded in Epon 812 or Poly 812, or 20 minutes for tissues embedded in Spurr's medium. The sections are rinsed, air dried, and mouted in Permount. The staining solution is very stable, and does not tend to form precipitates on the tissue. The stain brings excellent histological differentiation to nuclear, cytoplasmic, and extracellular components. Incorporation of the stain by elements within each tissue varies from intense to light with a subtle gradation of intermediate shades of purple and blue tones. For most cell structures the density of the stain parallels the electron density of that structure as seen under the electron microscope. For example, nucleoli and heterochromatin stain in dark purple while euterochromatin appear in a light blue shade. In all cases, the embedding media remains unstained. The bond between Stevenel's Blue and the tissues is stable, remaining unaltered by the mounting medium. It is also resistant to time-fading.</p>","PeriodicalId":76158,"journal":{"name":"Microscopica acta","volume":"83 2","pages":"117-21"},"PeriodicalIF":0.0,"publicationDate":"1980-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17221999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Tissue artefacts by the use of overheated forceps for paraffin embedding (author's transl)]. [使用过热的石蜡包埋钳进行组织人工制品(作者简介)]。
Microscopica acta Pub Date : 1980-05-01
K M Müller
{"title":"[Tissue artefacts by the use of overheated forceps for paraffin embedding (author's transl)].","authors":"K M Müller","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Histologic work-up of tissue specimens, especially from endoscopic biopsies, requires the use of fine forceps for orientation and dressing in fluid paraffin during the embedding procedure. These forceps are usually preheated over an open flame. If, however, smaller tissue particles are handled with overheated forceps, arteficial alterations may occur which are apt to hamper or falsify the histologic evaluation of the prepared section. Some typical tissue artefacts due to the handling with overheated forceps, are demonstrated with slides from liver biopsies. Recent experiences with an auxiliary instrument for paraffin embedding (Histostat of Vogel, designed by Ciplea) are reported. The forceps are kept at stable temperatures by immersion in fluid paraffin during the embedding procedure, thus excluding almost completely the risk of tissue artefacts by handling with overheated forceps.</p>","PeriodicalId":76158,"journal":{"name":"Microscopica acta","volume":"83 2","pages":"127-30"},"PeriodicalIF":0.0,"publicationDate":"1980-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18041129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A new preparation technique for scanning electron microscopy of skeletal muscle. 一种新的骨骼肌扫描电镜制备技术。
Microscopica acta Pub Date : 1980-03-01
L Józsa, M Järvinen, A Réffy
{"title":"A new preparation technique for scanning electron microscopy of skeletal muscle.","authors":"L Józsa,&nbsp;M Järvinen,&nbsp;A Réffy","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The authors describe a new preparation technique for scanning electron microscopic study of the internal structure of skeletal muscle cells. The specimens were fixed, dehydrated and imbibed with Epon or Durcupan--without polymerisation--and thereafter they were freeze-fractured.</p>","PeriodicalId":76158,"journal":{"name":"Microscopica acta","volume":"83 1","pages":"45-7"},"PeriodicalIF":0.0,"publicationDate":"1980-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18401331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Fluorescent staining of nuclear envelope coated with heparin. 肝素包被核包膜的荧光染色。
Microscopica acta Pub Date : 1980-03-01
K Grossgebauer
{"title":"Fluorescent staining of nuclear envelope coated with heparin.","authors":"K Grossgebauer","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Naturally and artificially devitalized cells of various origin can be loaded with heparin and subsequently stained with DAPI, a newer fluorochrome (4',6-diamidino-2-phenylindole), leading to a bright yellow fluorescence of the nuclear envelope. Depending on the cell type it is also possible to coat the outer cell membrane with heparin or other sulphated mucopolysaccharides and to achieve similar staining phenomena with DAPI.</p>","PeriodicalId":76158,"journal":{"name":"Microscopica acta","volume":"83 1","pages":"49-54"},"PeriodicalIF":0.0,"publicationDate":"1980-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17221997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Applications of image intensification to low level fluorescence studies of living cells. 图像增强在活细胞低水平荧光研究中的应用。
Microscopica acta Pub Date : 1980-03-01
G T Reynolds
{"title":"Applications of image intensification to low level fluorescence studies of living cells.","authors":"G T Reynolds","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Microscopic observations of weak fluorescence from living cells can be achieved by using image intensification techniques in situations where conventional film recording is not feasible. A brief description is given of experimental arrangements that have been used, involving recording the intensifier output alternately on film, or TV vidicons. References are given to more detailed descriptions of particular systems, and an example is presented of the detection of Ca++ in Haemanthus by means of the fluorescence of chlorotetracycline.</p>","PeriodicalId":76158,"journal":{"name":"Microscopica acta","volume":"83 1","pages":"55-62"},"PeriodicalIF":0.0,"publicationDate":"1980-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18041127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cryofixation of tissue specimens studied by cooling rate measurements and scanning electron microscopy. 用冷却速率测量和扫描电子显微镜研究组织标本的冷冻固定。
Microscopica acta Pub Date : 1980-03-01
K Zierold
{"title":"Cryofixation of tissue specimens studied by cooling rate measurements and scanning electron microscopy.","authors":"K Zierold","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The freezing velocity, the most important parameter for the quality of cryofixation of biological objects, was measured in frog liver specimens. The cooling course was found to depend on the size of the specimen, the specimen support and the cooling medium used (liquid nitrogen, supercooled nitrogen, Freon 12 and propane). The results were compared with scanning electron micrographs of freeze fractures cryofixed in the same manner: Propane yielded the highest cooling rates and, consequently, the best structural preservation. Morphologically similar results were obtained by combining Freon 12 and very small specimen supports. Generally, it can be said that the smaller both specimen and specimen support are, the higher is the freezing rate and the better the structural preservation. The findings are discussed with regard to further possibilities of improving the cryofixation of biological tissue.</p>","PeriodicalId":76158,"journal":{"name":"Microscopica acta","volume":"83 1","pages":"25-32"},"PeriodicalIF":0.0,"publicationDate":"1980-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18401330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Studies on structural changes of F-actin and myosin in living, intact and damaged muscle fibres by means of polarized ultraviolet fluorescence microscopy. 用偏振紫外荧光显微镜研究活肌纤维、完整肌纤维和损伤肌纤维中f -肌动蛋白和肌球蛋白的结构变化。
Microscopica acta Pub Date : 1980-01-01
Y S Borovikov
{"title":"Studies on structural changes of F-actin and myosin in living, intact and damaged muscle fibres by means of polarized ultraviolet fluorescence microscopy.","authors":"Y S Borovikov","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>By means of polarized ultraviolet fluorescence microscopy the structural changes of F-actin and myosin were discovered at the changing a functional state of a living muscle fibre and during spreading degeneration (Zenker's necrosis). The character of conformational changes of F-actin and myosin at activation, contraction, contracture and rigor is similar, but the number of changed macromolecules depends on a fibre state. At fibre local damage in its morphological intact parts there was found an alternation of zones, reflecting two states unusual for a fibre. During spreading degeneration these states transform into irreversible contracture and then into rigor. Similar changes were observed in muscle fibres obtained from denervated muscles.</p>","PeriodicalId":76158,"journal":{"name":"Microscopica acta","volume":"82 4","pages":"379-88"},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17942925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
首页 上一页
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整，为了账户安全，请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们：info@booksci.cn Book学术提供免费学术资源搜索服务，方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号：481959085
Book学术官方微信