{"title":"The effect of an experimental mouthrinse on enamel lesion remineralization in vitro.","authors":"J Arends, J de Vries, J Ruben","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>This paper describes the effect of a 2 ppm fluoride containing, slightly acidic mouthrinse (pH = 6) on the remineralization of enamel lesions in vitro. The rinse was applied twice daily for 2 min, on about 85 microns in depth artificial lesions. Artificial enamel lesions were formed at pH = 5 in a 6% CMC gel. The lesions were subsequently remineralized in vitro in a neutral remineralizing solution (1.5 mM Ca and 0.9 mM phosphate) with or without the application of the rinse. After 3 weeks the samples were evaluated by means of quantitative microradiography. The results showed that a statistically significant reduction in lesion depth occurred of 48 and 29% with or without rinse application, with respect to the original lesion depth. The effect of the rinse compared with no rinse decreased the lesion depth by 40%. The data indicate that application of a slightly acidic rinse with 2 ppm fluoride applied twice a day, stimulates the remineralization at the lesion front and does not lead to blocking of enamel pores observed after acidic, high level F applications (APF).</p>","PeriodicalId":75983,"journal":{"name":"Journal de biologie buccale","volume":"20 1","pages":"63-6"},"PeriodicalIF":0.0,"publicationDate":"1992-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12690283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Penetration of glutaric dialdehyde into human dentine as measured by changes in dentine microhardness and dentine dimensions.","authors":"E R van der Graaf, J J ten Bosch","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Microhardness indentation lengths were measured on human dentine sections unfixed and fixed with glutaric dialdehyde (GDA) at pH 7. Fixed dentine was found to be softer than unfixed dentine. Indentation length increased by approximately 10% after fixation in a buffered 5% GDA solution at pH 7. Using this effect the variation of indentation length in the direction of the penetrating GDA as a function of fixation time was measured. Describing the penetration in the mineralized matrix as diffusion in a plane sheet, a diffusion coefficient of 0.5.10(-12); m2/s was calculated. Dimensional changes of the dentine due to fixation were also measured. Dentine was found to expand in both the direction parallel (approximately 0.4%) and perpendicular (approximately 2%) to the tubules.</p>","PeriodicalId":75983,"journal":{"name":"Journal de biologie buccale","volume":"20 1","pages":"51-7"},"PeriodicalIF":0.0,"publicationDate":"1992-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12690281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of non-surgical periodontal therapy in an undergraduate dental clinic. Results one year following treatment.","authors":"I Vouros, A Konstantinidis, A Kirkou-Bata","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The aim of the present study was to evaluate the effectiveness of non-surgical treatment of moderately to severely advanced periodontitis in patients at the Department of Periodontology of the Aristotle University of Thessaloniki. The patients were treated by senior students of the Dental School under the supervision of specialized personnel. The conservative treatment consisted of oral hygiene instructions and repeated instrumentation (scaling and root planing) for a period of about 3 months. Seventeen patients with advanced periodontitis participated in the study. The following data were recorded: 1. Gingival index (Silness and Löe, 1963), 2. Plaque index (Löe and Silness 1964), 3. Bleeding on probing and 4. Pocket depth and probing attachment level. The measurements were taken by the same examiner four times, that is at baseline, immediately after completion of the conservative therapy, 6 months and 12 months after treatment. Measurements from single and multi-rooted teeth were divided into three groups with respect to the initial pocket depth (less than or equal to 3 mm, 4-6 mm, greater than or equal to 7 mm). The findings of the present study demonstrated that non-surgical therapy can achieved a marked reduction in bleeding on probing scores and pocket depth 12 months after treatment. A gain in probing attachment levels was found for sites with initially deep (greater than or equal to 7 mm) and moderate (4-6 mm) pockets, while in sites with initially shallow pockets (less than or equal to 3.0 mm), a loss of attachment level was established.</p>","PeriodicalId":75983,"journal":{"name":"Journal de biologie buccale","volume":"20 1","pages":"11-7"},"PeriodicalIF":0.0,"publicationDate":"1992-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12690980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Growth of the skeletal units of the rat mandible in acute renal failure experimental model.","authors":"M J Giglio, A Sanz, A Frid, T Leal, E Arrizurieta","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A study of the effect of acute renal failure (ARF) on the growth of the skeletal units of the mandible was performed. Male Wistar rats weighing 35-40 g were assigned to three groups. One of the groups received a basal choline-deficient diet ad libitum. A second group was pair-fed with the first one and was given a basal diet supplemented with 0.35 g choline chloride/100 g of diet. The third group was fed a basal diet plus 0.35 g choline chloride/100 g of diet ad libitum and used as the normal control. Body length, renal function parameters and measurements of the mandible were recorded at the end of a 12-day period on the different diets. Food intake and body weight were recorded every day. Plasma urea and creatinine concentrations markedly increased over the first 12 days of exposure to a choline-deficient diet. Significant increases in urine volume and significant depressions in urine osmolality were also observed. The average body weight and body length gains for rats given the choline-deficient diet were lower than those of control rats. There were no significant differences between the average body weight and body length of pair-fed and normal rats. All skeletal measurements of the mandible of the choline-deficient group differed significantly from those of the control group. These differences varied between 7% and 30%. The skeletal units of the mandible of the pair-fed group did not differ significantly from ad libitum controls. This study suggests that this rat model can be used for the investigation of the different alterations observed in uremia.(ABSTRACT TRUNCATED AT 250 WORDS)</p>","PeriodicalId":75983,"journal":{"name":"Journal de biologie buccale","volume":"20 1","pages":"19-23"},"PeriodicalIF":0.0,"publicationDate":"1992-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12690981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R J Gruythuysen, L W van der Linden, J H Wöltgens, W G Geraets
{"title":"Differences between primary and permanent teeth in posteruptive age dependency of radiological changes in enamel during the development of approximal caries.","authors":"R J Gruythuysen, L W van der Linden, J H Wöltgens, W G Geraets","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A longitudinal radiological study of children (N = 549) who participated in a comprehensive preventive maintenance program showed that caries related events in the approximal surfaces of permanent teeth differed from those in deciduous teeth. Changes in the approximal surfaces of the younger permanent teeth were more pronounced than of the older primary teeth and differed significantly from 1 year to 2.5 years. These findings can be explained by posteruptive maturation of tooth enamel.</p>","PeriodicalId":75983,"journal":{"name":"Journal de biologie buccale","volume":"20 1","pages":"59-62"},"PeriodicalIF":0.0,"publicationDate":"1992-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12690282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Demonstration of soluble IgG-Fc type II receptors (or s-Fc-gamma-II-R) in human whole saliva].","authors":"C Teillaud, W H Fridman, C Sautes","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Twenty human salivary samples from two groups of patients (with or without dental caries) were tested for the presence of soluble forms of Fc gamma receptors type II (sFc gamma RII): sFc gamma RII were detected by immunoblotting using a radiolabelled monoclonal antibody directed against human Fc gamma RII. These soluble forms specifically interact with the Fc portion of IgG and are produced by cells of the immune system (macrophages, neutrophils, Langerhans cells). This study showed that sFc gamma RII are present in human unstimulated saliva. The statistical analysis indicated that the relative amounts of sFc gamma RII in human unstimulated saliva are variable depending on the patient. No apparent association was found between the sFc gamma RII and the oral status of the patients.</p>","PeriodicalId":75983,"journal":{"name":"Journal de biologie buccale","volume":"20 1","pages":"3-10"},"PeriodicalIF":0.0,"publicationDate":"1992-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12560258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Concentration of antimicrobial proteins in human saliva. The effect of six months use of an antiplaque dentifrice on levels of antimicrobial proteins in unstimulated saliva from 102 adults.","authors":"G A Turner, C A Saxton, B Svatun","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Student nurses (aged 20-26 years) were assigned to two groups that were matched for plaque levels and gingival health. For six months, one group used a standard fluoride dentifrice while the other used an identical dentifrice to which zinc citrate (1%, w/w) and Triclosan (0.2%, w/w) had been added. Levels of natural antimicrobial proteins (lysozyme, lactoferrin, salivary peroxidase and Immunoglobulin A) in whole, unstimulated saliva taken from the students at the start and on completion of the six months were measured. No statistically significant differences were found in the levels of antimicrobial proteins in saliva between the test and placebo groups.</p>","PeriodicalId":75983,"journal":{"name":"Journal de biologie buccale","volume":"19 4","pages":"267-70"},"PeriodicalIF":0.0,"publicationDate":"1991-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12953049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A S Ligthelm-Bakker, B Prahl-Andersen, E Wattel, I H Uljee
{"title":"A new method for locating anterior skeletal landmarks from soft tissue measurements.","authors":"A S Ligthelm-Bakker, B Prahl-Andersen, E Wattel, I H Uljee","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Clinical examination of human profiles provides some idea of the localization of hard tissue landmarks, but is unreliable because of large variability in thickness of the soft tissues. In this investigation an instrument was developed to measure the thickness of the lips. Linear regression was applied to estimate cephalometric hard tissue landmarks from those in the soft tissues. By adding information from direct clinical measurements to the model for estimating hard tissue landmarks from soft tissue landmarks, the correlation between the digitized (actual) and estimated or calculated landmarks was significantly improved.</p>","PeriodicalId":75983,"journal":{"name":"Journal de biologie buccale","volume":"19 4","pages":"283-90"},"PeriodicalIF":0.0,"publicationDate":"1991-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12953051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[The expression of various cytokeratins by epithelial cells of calcified odontogenic cysts].","authors":"M Gordeeff, S Clergeau-Guerithault","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The calcifying odontogenic cyst (COC) is a rare lesion without specific clinical characteristics. Its diagnosis is essentially histopathological showing the presence of ghost cells associated with narrow squamous epithelium, the basal strata of which consist of clearly delineated cells differentiating in areas of the stellate reticulum in a similar way to ameloblastoma. The results of ultrastructural observations of the ghost cells as well as histochemical and immunohistochemical studies suggest that they are the sites of abnormal keratinisation. The aim of this study of one COC mas to locate the stages in epithelial maturation associated with the formation of ghost cells. Six monoclonal antibodies were used; three with a wide spectrum (KL1, AE3, AE1), two with a narrow spectrum against high molecular weight cytokeratins (AE2, AE8) and one against vimentin (M725). Histopathological examination of the COC revealed three different types of cells in the epithelial lining and the epithelial islands; small basal cubic cells surrounding larger ones placed centrally or suprabasally; balloon shaped cells or flattened ghost cells rolled up on themselves to resemble keratinising pearls, or cornifying cells. The ghost cells and cornifying cells had an altered distribution of their cytokeratins demonstrated by the absence of staining of antibodies against cytokeratins. The differentiated cells adjacent to them showed cytokeratins typical of squamous epithelium rather than those associated with the process of keratinization. The coexistence of cornifying and ghost cells testifies to the great potential of odontogenic epithelium to form numerous epithelial islands.</p>","PeriodicalId":75983,"journal":{"name":"Journal de biologie buccale","volume":"19 4","pages":"319-28"},"PeriodicalIF":0.0,"publicationDate":"1991-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12887776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H Michelet, B Fontaniere, H Magloire, B M Durand, E Tabone
{"title":"Extracellular matrix and intermediate filaments in the first stages and repair of experimental gingivitis in man.","authors":"H Michelet, B Fontaniere, H Magloire, B M Durand, E Tabone","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>By the indirect immunoperoxidase labelling procedure the expressions of type I and III collagens, laminin and fibronectin and of KL1 cytokeratin and vimentin were examined in the first stages and repair of experimental gingivitis in young subjects. A two month longitudinal study was performed using the tissue from buccal marginal gingival biopsies of four subjects taken sequentially at five specific times: before and during plaque accumulation, and during plaque elimination. The sites examined microscopically were the coronal half of the junctional epithelium and the underlining infiltrated connective tissue fraction. No clinical change could be observed during the study period. Histological examination showed reversible cellular changes during the accumulation of plaque. There were increases in vascularization and cellularity and loss of collagen. They recovered 56 days after plaque elimination their baseline level. Electron microscopic examination showed myofibroblastic aspects in some fibroblasts. The changes in the expression of laminin, fibronectin and KL1 in the J.E. might be due to a proliferation rate enhancement, and suggest an adaptation to the alterations brought about by the inflammatory process. They also reinforce the hypothesis that this epithelium resembles a developmental tissue. Type I collagen demonstrated the \"collagen loss-repair\" cycle shown in connective tissue by the histological study. The rise in type III collagen and the vimentin fall, both at the initial stage, suggest that these protein profiles may yield information for clinical research purposes during the very early inflammatory process. The variations in fibronectin indicate its key role in the early inflammatory and repair processes. Finally, the variations in matrix and cytoskeletal proteins variations, as well as the morphological modifications observed, were nearly all reversible.</p>","PeriodicalId":75983,"journal":{"name":"Journal de biologie buccale","volume":"19 4","pages":"329-41"},"PeriodicalIF":0.0,"publicationDate":"1991-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12888319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}