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Journal de biologie buccale最新文献

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Peroxidase activity loss after filtration and centrifugation of whole saliva. Influence of citrate. 全唾液过滤和离心后过氧化物酶活性损失。柠檬酸盐的影响。
Journal de biologie buccale Pub Date : 1992-06-01
A Vanden Abbeele, P Courtois, M Pourtois
{"title":"Peroxidase activity loss after filtration and centrifugation of whole saliva. Influence of citrate.","authors":"A Vanden Abbeele,&nbsp;P Courtois,&nbsp;M Pourtois","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Filtration or centrifugation are two methods frequently used to clarify human whole saliva. This study compares both the drop in peroxidase activity following filtration (49.3% +/- 16.4%; N = 12) or centrifugation (33.6% +/- 15.9%; N = 6) and the drop in total protein concentration after filtration (33.9% +/- 13.4%) and centrifugation (23.6% +/- 8.7%). After filtration, the loss was not only due to retention of the large particle-bound fraction but also to adsorption on the filter membranes. This adsorption could be inhibited by a previous dilution of saliva in a citrate buffer, which also gave rise to active subunits with a molecular weight less than or equal to 100 kDa.</p>","PeriodicalId":75983,"journal":{"name":"Journal de biologie buccale","volume":"20 2","pages":"91-6"},"PeriodicalIF":0.0,"publicationDate":"1992-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12811011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Age-adjusted limits of fluoride intake to minimize the prevalence of fluorosis. 按年龄调整的氟化物摄入量限制，以尽量减少氟中毒的流行。
Journal de biologie buccale Pub Date : 1992-06-01
T M Marthaler
{"title":"Age-adjusted limits of fluoride intake to minimize the prevalence of fluorosis.","authors":"T M Marthaler","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The range of optimal fluoride intake for adults, 1.5 to 4 mg per day, was adjusted for children and adolescents aged 1 to 19 by relating these limits to body weight and energy intake. Limits related to weight agreed well with the usually recommended ranges for supplemented fluorides. However, for children up to 6 years of age limits related to energy intake were twice as high as those related to body weight. These differences diminished with increasing age and were small between 16 and 19 years of age. New conservative limits were based on an interval for adults ranging from 1.45 to 2.9 mg fluoride per day. The new age-adjusted limits were compatible with most current recommendations.</p>","PeriodicalId":75983,"journal":{"name":"Journal de biologie buccale","volume":"20 2","pages":"121-7"},"PeriodicalIF":0.0,"publicationDate":"1992-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12811103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Antimicrobial factors of saliva in relation to dental caries and salivary levels of mutans streptococci. 唾液中的抗菌因子与龋齿和变异链球菌唾液水平的关系。
Journal de biologie buccale Pub Date : 1992-06-01
J Tenovuo, H Jentsch, T Soukka, L Karhuvaara
{"title":"Antimicrobial factors of saliva in relation to dental caries and salivary levels of mutans streptococci.","authors":"J Tenovuo,&nbsp;H Jentsch,&nbsp;T Soukka,&nbsp;L Karhuvaara","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The possible association between salivary non-immunoglobulin (lysozyme, lactoferrin, hypothiocyanite, agglutinins) or immunoglobulin (total IgA, anti-Streptococcus mutans IgA) antimicrobial factors, and the prevalence of dental caries was studied in 59 young adults. These antimicrobial factors were also analysed in relation to the salivary levels of mutans streptococci (MS). The amount of MS correlated significantly (+0.31, p less than 0.05) with the number of initial caries lesions (Di) but not with other caries indices (DMFT, DMFS, DS). The group with no Di (N = 17) had significantly (p less than 0.05) more hypothiocyanite (HOSCN/OSCN-) and anti-S. mutans IgA antibodies in whole saliva than those with initial caries lesions (N = 42). None of the antimicrobial factors alone showed any significant association with salivary MS counts. Our results suggest that HOSCN/OSCN- and anti-S. mutans IgA may be involved in the prevention of the early phases of dental caries.</p>","PeriodicalId":75983,"journal":{"name":"Journal de biologie buccale","volume":"20 2","pages":"85-90"},"PeriodicalIF":0.0,"publicationDate":"1992-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12811106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Metabolism of glycogen in submandibular glands of rats. Alteration by NaF. 大鼠颌下腺糖原代谢的研究。由NaF变化
Journal de biologie buccale Pub Date : 1992-06-01
J Nicolau, D M Ribeiro
{"title":"Metabolism of glycogen in submandibular glands of rats. Alteration by NaF.","authors":"J Nicolau,&nbsp;D M Ribeiro","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Submandibular glands of rats injected with NaF solution (10 mg F-/kg body weight) were analysed for glycogen content and phosphorylase activity after various time intervals. In contrast to what has been reported for the liver, sodium fluoride caused increased glycogen content. Phosphorylase (a and total) activity was not affected, suggesting a different mechanism of action of F- in the submandibular gland. In vivo experiments demonstrated stimulation of glycogenesis.</p>","PeriodicalId":75983,"journal":{"name":"Journal de biologie buccale","volume":"20 2","pages":"97-102"},"PeriodicalIF":0.0,"publicationDate":"1992-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12811012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Adsorption of dentine proteins onto spheroidal calcium phosphate in a neutral and in an acidic environment. 中性和酸性环境下牙本质蛋白在球状磷酸钙上的吸附。
Journal de biologie buccale Pub Date : 1992-06-01
W D Boonstra, J J ten Bosch, J Arends
{"title":"Adsorption of dentine proteins onto spheroidal calcium phosphate in a neutral and in an acidic environment.","authors":"W D Boonstra,&nbsp;J J ten Bosch,&nbsp;J Arends","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The adsorption of four different dentine protein fractions onto hydroxyapatite (HAP) was investigated in a nearly neutral (pH 6.7) and in an acidic environment (pH 5.0). The soluble dentine proteins were isolated according to their extractability in successively: 4M guanidine-HCl (GuHCl), 0.5M acetic acid pH 3 (after extraction with GuHCl), and 0.01M EDTA pH 7.4 and 0.1M EDTA pH 7.4 (after complete demineralization with acetic acid). The amounts isolated were 1.2, 3.3, 6.1 and 3.7 mg protein per gram dentine, respectively. The GuHCl extractable proteins exhibited the lowest affinity and the acetic acid extractable proteins the highest affinity for HAP. Acetic acid extractable proteins, were significantly less adsorbed than the other protein fractions at pH 5.0. The maximum adsorbed amount of these proteins increased with decreasing pH which indicated that all the soluble dentine proteins, except perhaps those that one GuHCl-extractable have to be considered as potential demineralization inhibitors.</p>","PeriodicalId":75983,"journal":{"name":"Journal de biologie buccale","volume":"20 2","pages":"111-6"},"PeriodicalIF":0.0,"publicationDate":"1992-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12811101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Spontaneous recovery from growth disturbances and osteopenia induced by a high carbohydrate diet in hamsters. 高碳水化合物饮食诱导的仓鼠生长障碍和骨质减少的自发恢复。
Journal de biologie buccale Pub Date : 1992-06-01
C de Tessieres, J L Saffar
{"title":"Spontaneous recovery from growth disturbances and osteopenia induced by a high carbohydrate diet in hamsters.","authors":"C de Tessieres,&nbsp;J L Saffar","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Femoral osteopenic changes characterized by a fall in metaphyseal trabecular bone volume (TBV) have been previously reported in hamsters fed a high carbohydrate diet for 45 days. In the present study the effects of the diet were assessed after 90 days. Serum P was significantly reduced (p less than 0.05) in experimental animals; serum Ca was unaltered. Disturbances in body growth had occurred as shown by a reduction in body weight gain (p less than 0.05) in the first 45 days of the experiment and by a decrease in femoral size (p less than 0.02). Osteopenia had also developed: the epiphyseal TBV decreased (p less than 0.05) as well as the thickness of both epiphyseal and metaphyseal trabeculae (p less than 0.001). However signs of partial recovery from this condition were observed as shown by an increased body weight gain in the last 45 days of the experiment (p less than 0.02) and lack of difference in metaphyseal TBV, assumed to be the more labile, between the 2 groups. Moreover bone formation significantly increased along metaphyseal trabeculae and the endosteal aspect of the cortices.</p>","PeriodicalId":75983,"journal":{"name":"Journal de biologie buccale","volume":"20 2","pages":"117-20"},"PeriodicalIF":0.0,"publicationDate":"1992-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12811102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Evaluation of a histochemical method specific for myosin ATPase activity in the masticatory muscles of the rat]. [大鼠咀嚼肌肌球蛋白atp酶活性特异性组织化学方法的评价]。
Journal de biologie buccale Pub Date : 1992-06-01
G Didier, A Westphal
{"title":"[Evaluation of a histochemical method specific for myosin ATPase activity in the masticatory muscles of the rat].","authors":"G Didier,&nbsp;A Westphal","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A biological study of masticatory muscle behaviour (Divry and Westphal, 1991) suggested the analysis of physiologic correlates (biologic parameters related to a behavioural event) such as histochemical reactions of muscular fibres studied by M-ATPase and SDH activities. For such investigations, routine methods are needed. In the present study, a modification of the original method of Tunell and Hart (1977) was used, in which three features of the original alkaline preincubation method (composition, incubation time and pH) were modified. These allowed a single step differentiation of the various fibre types found in rat masticatory muscles, for which the classical technics gave only a weak contrast, not suitable for image analysis. Acid preincubation was also tested but failed to give new information. By combining this modified technic with SDH staining (Nachlas, 1957) a classification of fibres into 12 theoretical types was proposed.</p>","PeriodicalId":75983,"journal":{"name":"Journal de biologie buccale","volume":"20 2","pages":"103-9"},"PeriodicalIF":0.0,"publicationDate":"1992-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12551174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Antifungal varnish in the treatment of denture stomatitis]. 抗真菌清漆在义齿口炎治疗中的应用
Journal de biologie buccale Pub Date : 1992-03-01
P Carlino, R Lang, E Budtz-Jorgensen
{"title":"[Antifungal varnish in the treatment of denture stomatitis].","authors":"P Carlino,&nbsp;R Lang,&nbsp;E Budtz-Jorgensen","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The efficacy of a single-dose application of miconazole varnish in the treatment of denture stomatitis was compared with miconazole gel applied three times/day for 15 days. Among 288 wearers of complete dentures 50 patients severely affected by denture stomatitis and heavily colonized by Candida, were selected. The patients were assigned randomly to two groups: a miconazole varnish group and a gel group. All patients were examined 7 times: before starting the treatment (day 0); during treatment (day 3, 7 and 14); after treatment (day 21, 28 and 35). At each examination a photograph of the palatal mucosa was obtained and quantitative cultures of Candida from the lesions and the fitting denture surface were performed. At day 14 the inflammation was reduced to the same degree in the two groups of patients. A comparison of the antifungal effect, i.e. reduction of the yeast score, showed no significant difference between the two groups. Maximum reduction of the number of yeasts was obtained at day 14. From that moment, recolonization started; thus, about 60% of the treated patients whether it was with the gel or the varnish was positive of Candida by day 35. In conclusion, the present study showed no difference in the clinical and antifungal effect of a single-dose application of miconazole varnish compared with miconazole gel. The varnish seemed to be better with respect to the posology as the total dose of miconazole is minimal and only one application is necessary. Treatment with the miconazole varnish seems particularly indicated in debilitated and non-cooperative patients suffering from Candida-associated denture stomatitis.</p>","PeriodicalId":75983,"journal":{"name":"Journal de biologie buccale","volume":"20 1","pages":"45-50"},"PeriodicalIF":0.0,"publicationDate":"1992-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12690280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Gingival hyperplasia secondary to the use of calcium antagonists: analysis]. [钙拮抗剂继发牙龈增生:分析]。
Journal de biologie buccale Pub Date : 1992-03-01
P Sauget, R A Monteil, P Morand, R Loubiere, P Lapalus, C Haudebourg
{"title":"[Gingival hyperplasia secondary to the use of calcium antagonists: analysis].","authors":"P Sauget,&nbsp;R A Monteil,&nbsp;P Morand,&nbsp;R Loubiere,&nbsp;P Lapalus,&nbsp;C Haudebourg","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Calcium antagonists are drugs restricting transmembrane calcium delivery. They possess a wide range of action against vasoconstriction and spastic reactions and were therefore initially recommended for the treatment of angina pectoris. With the increasing number and classes of calcium antagonists new therapeutic indications have emerged. Cases of gingival hyperplasia associated with their use are repeatedly reported, therefore the question deserves to be restated. The aim of the present study was to discuss the clinical, pathologic and pathogenetic features bases on an investigation carried out in a Department of Cardiology, on a case observation and on review of published cases in the international literature.</p>","PeriodicalId":75983,"journal":{"name":"Journal de biologie buccale","volume":"20 1","pages":"25-32"},"PeriodicalIF":0.0,"publicationDate":"1992-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12690982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A switch in cytokeratin expression and intermediate filament organization associated with epithelial stratification. 与上皮分层相关的细胞角蛋白表达和中间丝组织的开关。
Journal de biologie buccale Pub Date : 1992-03-01
A H Shabana, R Khattab, M H Sawaf, N Forest, J P Ouhayoun
{"title":"A switch in cytokeratin expression and intermediate filament organization associated with epithelial stratification.","authors":"A H Shabana,&nbsp;R Khattab,&nbsp;M H Sawaf,&nbsp;N Forest,&nbsp;J P Ouhayoun","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Low density gingival epithelial cells were cultured on the side of glass slides facing rat's tail collagen lattices. Under these conditions and in the presence of physiological level of calcium, colony formation was enhanced and stratification was slowed down. The strong attachment of the cells to glass slides permitted immunocytochemical examination of cytokeratin (CK) expression and their organization within individual cells during the different stages of epithelial maturation. The present results showed that during the stage of cell migration and colony formation, the cells express the same set of cytokeratins (basal cell marker 14, simple epithelial markers 8, 18 and 19, and marker of hyperproliferation 16) which forms a well-defined network of organized filaments. At the stratification stage, the filament network became dense by the additional expression of the markers of differentiation in non-keratinized stratified epithelia (CK 4 and 13). These appeared once individual cells started to overlap the basal cells, a period during which the cell-temporarily changed morphology. Whilst the suprabasal cells exhibited dense filament network labelled for CK 4 and 13, the density of labelled filaments for CK 14, 8 and 18 was much lower, indicating that these cells contained newly-formed filaments lacking the basal and simple epithelial keratins. The simple epithelial cytokeratins became weakly labelled in older cultures. The uncoupling of paired expression of cytokeratins 4 and 13 was observed in non-colony forming aged cells. This provides an example of altered program of cytokeratin expression during epithelial maturation.</p>","PeriodicalId":75983,"journal":{"name":"Journal de biologie buccale","volume":"20 1","pages":"33-43"},"PeriodicalIF":0.0,"publicationDate":"1992-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12554395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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