Russian Journal of Bioorganic Chemistry最新文献

{"title":"A Comparative Study of the Rheological Behavior of Solutions of the Chitin-Glucan Complex from the Fruit Bodies of Armillaria mellea in Acetic and Hydrochloric Acids","authors":"D. V. Minakov,&nbsp;D. V. Chashchilov,&nbsp;A. A. Minakova,&nbsp;V. I. Markin","doi":"10.1134/S1068162024070288","DOIUrl":"10.1134/S1068162024070288","url":null,"abstract":"<p><b>Objective:</b> The rheological properties of the chitin-glucan complex (CGC) in aqueous solutions of hydrochloric and acetic acids were studied. The CGC sample was isolated from the fruit bodies of the honey mushrooms (Armillaria mellea), biotechnologically obtained from an easily renewable plant raw material. <b>Methods:</b> The flow behavior of the solutions of the chitin-glucan complex with a concentration of 1, 5, and 10 wt % in hydrochloric acid and of 1, 3, and 5 wt % in acetic acid was studied by the method of rotational viscometry in the shear rate range of 10 to 1000 s^–1 at temperatures from 20 to 50°C. <b>Results and Discussion:</b> The values of the rheological coefficients of the Ostwald de Waele power-law equation were determined. Viscosity anomalies indicating non-Newtonian flow behavior were revealed. The high viscosity of the solutions may be due to the presence of an internal supramolecular structure in the solutions of the chitin-glucan complex. The predominantly pseudoplastic nature of the rheological behavior of the studied solutions was shown. The flow index in this case varies from 0.18 to 0.79 for the solutions of the chitin-glucan complex in hydrochloric acid and from 0.01 to 0.47 for those in acetic acid with a concentration of 3 to 5 wt %. The pseudoplastic flow mechanism for such solutions can be explained by the destruction of the internal structure of the solution with increasing shear load. The dilatant nature of the flow of 1 wt % solutions of CGC in acetic acid at a shear rate of 10 to 100 s^–1, with the flow index ranging from 1.28 to 1.57, was also revealed. <b>Conclusions:</b> Dilatant behavior may be explained by the predominance of the processes of the formation of a new internal structure in the solution over those of the destruction of the existing structure in the solution at a low concentration of the chitin-glucan complex. The influence of temperature on the rheological behavior of the solutions is strongly distorted by the contribution from other factors.</p>","PeriodicalId":758,"journal":{"name":"Russian Journal of Bioorganic Chemistry","volume":"50 7","pages":"2910 - 2917"},"PeriodicalIF":1.1,"publicationDate":"2024-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142870436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of the Interaction of Water–Glycerin Mixture and Sawdust of Larch Larix sibirica Ledeb (Pinaceae)","authors":"E. L. Akim,&nbsp;A. A. Pekaretz,&nbsp;P. M. Mukhina,&nbsp;O. A. Erokhina,&nbsp;O. V. Fedorova,&nbsp;S. Z. Rogovina,&nbsp;A. A. Berlin","doi":"10.1134/S1068162024070094","DOIUrl":"10.1134/S1068162024070094","url":null,"abstract":"<p><b>Objective:</b> The interaction of the eutectic plasticizer—aqua complex “glycerin–water” with sawdust of larch wood of two fractional compositions was studied: a fine fraction (wood flour) and a coarse fraction (sawdust) with a particle size of 1−3 mm. <b>Methods:</b> The methods of optical microscopy were used to study the surface properties of samples, to measure the dynamic viscosity of spent solutions on a Brookfield viscometer; to measure the VGS retention index, a modified Jaime water retention technique was used. The obtained data are compared with the results of previous studies, in which it was shown that arabinogalactan (AG) performs the functions of a eutectic plasticizer in the structure of larch wood and is in the form of an aqua complex «AG–water». <b>Results and Discussion:</b> It was found that the amount of the attached aqua-complex—“glycerin−water” to the samples of larch sawdust corresponds to the amount of AG previously extracted from sawdust. The process of replacing the aqua-complex “AG−water” with the aqua-complex “glycerin–water” is analyzed on the diagram of relaxation states “two polymers–solvent.” The experimental results are interpreted on the basis of previously obtained data on the effect of water and glycerin on the relaxation state of polymer components of wood. <b>Conclusions:</b> The fundamental difference between the two eutectic plasticizers is demonstrated: if, when drying larch wood, the destruction of the aqua-complex “AG−water” leads to the transfer of polymer components of wood from a highly elastic to a glassy state, then when using the aqua-complex “glycerin−water” (VGS), water is removed during drying, and the remaining glycerin ensures the preservation of polymer components of wood in a highly elastic state. The possible technological aspects of the revealed patterns are considered.</p>","PeriodicalId":758,"journal":{"name":"Russian Journal of Bioorganic Chemistry","volume":"50 7","pages":"2787 - 2793"},"PeriodicalIF":1.1,"publicationDate":"2024-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142870273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biologically Active Substances of Agastache rugosa (Lamiaceae) Introduced in Western Siberia","authors":"N. E. Kolomiets,&nbsp;O. N. Shplis","doi":"10.1134/S1068162024070276","DOIUrl":"10.1134/S1068162024070276","url":null,"abstract":"<p><b>Objective:</b> The chemical composition of the bioactive substances of various parts and organs of <i>Agastache rugosa</i> introduced in Western Siberia was studied. Samples of Agastache rugosa were collected by the authors in July–August, 2021–2022, in the territory of Tomsk oblast. Aqueous and aqueous-alcoholic extracts were obtained from the raw materials. <b>Methods:</b> The studies were conducted using PC, TLC, UV-spectroscopy, and titrimetry. The component composition of the essential oils was analyzed by GLC-MS on an Agilent 6890 gas chromatograph with quadrupole mass spectrometer (HP MSD 5973N). <b>Results and Discussion:</b> About 50 individual components were found, 30 of them for the first time. Vitamins (ascorbic acid, carotenoids), polysaccharides, phenolic compounds (flavonoids, phenolcarboxylic acids, tannins), and essential oil were found in all organs of the plant. Saponins were present in the flowers and the herb, but not in the fruits. The presence of acacetin, apigenin, quercetin, tilianin, rosmarinic acid, caffeic acid, and chlorogenic acid was detected. <b>Conclusions:</b> Differences in the contents of bioactive substances in different organs were revealed. The dominant groups in different parts and organs of the plant were essential oil, phenolcarboxylic acids, carotenoids, and ascorbic acid. Flavonoids and chlorophyll in all organs of Agastache rugosa species belong to the group of minor bioactive substances.</p>","PeriodicalId":758,"journal":{"name":"Russian Journal of Bioorganic Chemistry","volume":"50 7","pages":"2890 - 2896"},"PeriodicalIF":1.1,"publicationDate":"2024-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142870282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Esterification of Betulin 3-Acetate in Melts of the Maleic and Levulinic Acids","authors":"V. A. Levdanskiy,&nbsp;N. V. Garyntseva,&nbsp;A. V. Levdanskiy","doi":"10.1134/S106816202407029X","DOIUrl":"10.1134/S106816202407029X","url":null,"abstract":"<p><b>Objective:</b> Esters of betulin containing residues of bioactive aromatic and aliphatic acids are of interest to the chemical and pharmaceutical industry as hepatoprotectors, anti-inflammatory, antiulcer and immunomodulatory substances. The development of new efficient, ecological and economical methods for the synthesis of betulin esters is an urgent task. <b>Methods:</b> The structure of the resulting betulin esters was determined using IR and NMR spectroscopy, and the composition was determined by elemental analysis. <b>Results and Discussion:</b> A new “green” method for the production of betulin 3-acetate-28-maleate and betulin 3-acetate-28-levulinate has been developed. For the first time, esterification of betulin 3-acetate with melts of maleic acid and levulinic acids was carried out at a temperature of 185–200°C for 5–7 min to obtain betulin 3-acetate-28-maleate and betulin 3-acetate-28-levulinate, respectively. <b>Conclusions:</b> The advantages of the developed method for the synthesis of 3-acetate-28-maleate and 3-acetate-28-levulinate of betulin in comparison with the known ones are: the synthesis is carried out in the absence of harmful and hazardous solvents (pyridine, methylene chloride, chloroform); the duration of synthesis is reduced from 15–40 h to 5–7 min. Maleic acid is used instead of maleic anhydride on the preparation of betulin 3-acetate-28-maleate.</p>","PeriodicalId":758,"journal":{"name":"Russian Journal of Bioorganic Chemistry","volume":"50 7","pages":"2918 - 2921"},"PeriodicalIF":1.1,"publicationDate":"2024-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142870423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a One-Step Method for Producing Allobetulin from Birch Outer Bark and Study of Its Antioxidant Activity","authors":"E. S. Skurydina,&nbsp;N. Yu. Vasil’eva,&nbsp;S. A. Kuznetsova,&nbsp;N. M. Titova,&nbsp;B. N. Kuznetsov","doi":"10.1134/S1068162024070252","DOIUrl":"10.1134/S1068162024070252","url":null,"abstract":"<p><b>Objective:</b> Birch bark contains extractives of lupane series, that have pharmacological activity and are of interest for obtaining new medicines. The predominant component of the birch outer bark extracts is pentacyclic triterpene alcohol, betulin. Allobetulin (19,28-epoxyoleanan-3-ol), a pentacyclic oleanane-type triterpenoid, is an isomer of betulin. Allobetulin and its derivatives are nontoxic compounds having antiviral, antimicrobial, and antitumor activities. <b>Methods:</b> The composition of the allobetulin sample after recrystallization from isopropanol was confirmed by elemental analysis, and its structure, by IR and ¹H NMR spectroscopy. The antioxidant properties of allobetulin were tested<i> in vivo</i> and <i>in vitro</i>. <b>Results and Discussion:</b> In the present study, a new one-step method was developed for producing allobetulin directly from birch outer bark by combining the stages of betulin extraction with ethanol and isomerization into allobetulin in the presence of an orthophosphoric acid catalyst. The effect of the duration of the birch outer bark treatment with ethanol and of the H3PO4 content on the allobetulin yield was evaluated. The highest yield of crude allobetulin, 35% of the weight of absolutely dry bark (a.d.b.), was achieved at a process duration of 8 h in the presence of 5% H₃PO₄ and also at a process duration of 15 h in the presence of 3% H₃PO₄. <b>Conclusions:</b> The yield of recrystallized allobetulin was ~22% of the weight of a.d.b.</p>","PeriodicalId":758,"journal":{"name":"Russian Journal of Bioorganic Chemistry","volume":"50 7","pages":"2866 - 2873"},"PeriodicalIF":1.1,"publicationDate":"2024-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142870427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Elemental Chemical Composition of the Larix Sibirica Needles in the Predbaikalia","authors":"L. V. Afanaseva,&nbsp;O. V. Kalugina,&nbsp;T. M. Kharpukhaeva","doi":"10.1134/S1068162024070070","DOIUrl":"10.1134/S1068162024070070","url":null,"abstract":"<p><b>Objective:</b> The study aim to investigate the features of macro- and microelements accumulation in the <i>Larix sibirica</i> needles depending on the growth conditions in the Predbaikalia.<b> Methods:</b> To determine the concentrations of inorganic elements in the needles, the inductively coupled plasma-mass spectrometry (ICP-MS) technique was used. The dry pine needles were mineralized in a muffle furnace; the ash was dissolved in 0.1 M nitric acid. The mobile forms of microelements were extracted from the soil using 1 M HCl. <b>Results and Discussion:</b> In the results, it is found that most of the elements in the needles exhibited low (N, K, Mg, P, Na, Zn, F, Sc, Sb, Pb, Mo, Be) and medium (Ca, S, Al, Fe, Ti, Cu, Ni, As, Cr, V, Cd, Ce, Y) levels of concentration variability. High coefficients of variation (&gt; 36%) were found for Mn, Sr, Co, and Li; their concentrations in the needles depended on the growth conditions (forest type), including the level of mobile forms in the soil. It was shown that <i>L. sibirica</i> is characterized by potassium-nitrogen type of mineral nutrition. The sequence of macroelements in the accumulation series was constant; the group of ultramicroelements (<i>n</i> × 10^–6 mg/kg) showed a high mobility. The ratios of the concentrations of the physiologically important elements (N/P/K, P/N, K/Ca, K/Mg, K/P, Ca/Mg, Fe/Mn, Cu/Zn, Fe/Zn, Cu/Mo) in the dry matter of the needles were determined; the dependence of several ratios on the growth conditions was revealed. <b>Conclusions:</b> This research provides valuable information on the chemical composition of the one of the main forest-forming tree species. The data obtained can be used as a basis for comparative analysis of the balance of major nutrients in <i>L. sibirica</i> in other areas and to address ecological challenges.</p>","PeriodicalId":758,"journal":{"name":"Russian Journal of Bioorganic Chemistry","volume":"50 7","pages":"2818 - 2825"},"PeriodicalIF":1.1,"publicationDate":"2024-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142870268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dynamics of the Formation of the Phenolic Redox System of Coniferous Plants on the Example of Scots Pine (Pinus Sylvestris L.)","authors":"K. G. Bogolitsyn,&nbsp;M. A. Gusakova,&nbsp;A. A. Krasikova,&nbsp;S. S. Khviyuzov,&nbsp;N. V. Selivanova,&nbsp;N. A. Samsonova,&nbsp;M. A. Pustynnaya","doi":"10.1134/S1068162024070136","DOIUrl":"10.1134/S1068162024070136","url":null,"abstract":"<p><b>Objective:</b> This study aims to investigation of the mechanisms of the redox processes in the formation of phenolic secondary metabolites in higher coniferous plants in natural growing places, using Scots pine as an example. <b>Methods:</b> The composition and reactivity of the phenolic fraction of the phenol-quinone redox system of the cell wall and the quantitative change of the pigment apparatus of pine needles during the growing season were investigated using a complex of physic-chemical methods (UV spectrophotometry, HPLC, redox-metry). <b>Results and Discussion:</b> It is shown that changes in the quantitative and qualitative composition of the phenolic compounds fraction that determine the redox state of the plant matrix occurs in plant tissue throughout the growing season. It is noted that dynamic self-regulation processes involving a complex consisting of phenolic compounds, pigments and enzymatic systems are a common pattern at all stages of plant development, ensuring that they perform protective functions of the photosynthetic apparatus of the needles of evergreen coniferous trees in boreal regions. The redox systems of the phenolic compounds are formed due to donor-acceptor interactions in photosystems I and II with participation of photosynthetic pigments. The implementation of the protective functionality of phenolic compounds of the pine tree greenery is enabled by high values of the effective oxidation potential (up to 780 mV) and the free energy of oxidation (–150 kJ/molOHph). <b>Conclusions:</b> This research provides new experimental data on the changes in the contents of phenolic secondary metabolites and the pigment complex of the needles during the growing season for coniferous plants, using the example of Scots pine (<i>Pinus sylvestris</i>).</p>","PeriodicalId":758,"journal":{"name":"Russian Journal of Bioorganic Chemistry","volume":"50 7","pages":"2794 - 2803"},"PeriodicalIF":1.1,"publicationDate":"2024-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142870283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biotechnological Methods for Obtaining Biologically Active Compounds of Rhodiola Rosea L. (A Review)","authors":"D. A. Zhdanov,&nbsp;T. K. Ryazanova,&nbsp;V. A. Kurkin,&nbsp;A. V. Kurkina,&nbsp;V. B. Braslavsky","doi":"10.1134/S1068162024070112","DOIUrl":"10.1134/S1068162024070112","url":null,"abstract":"<p>Due to its rich chemical composition including unique biologically active compounds the gold root (<i>Rhodiola rosea</i> L.) is still of interest to researchers all over the world. However, deficiency of the raw material base and metabolome variability are determination the modern development ways of pharmacologically active substances <i>in vitro</i> obtaining. The article summarizes and systematizes the information about of Rhodiola rosea biologically active compounds obtaining, which are most often obtaining from callus tissues. Callus tissue is usually obtaining from leaf explants, and Murashige and Skoog medium with plant growth regulators in various combinations and concentrations is most commonly used for its induction. At the same time, the tissue growth efficiency and target compounds accumulation depends on the intact plant genotype, the precursors presence of these compounds in the medium, elicitors, as well as external influence factors. Recently, a promising direction is the transgenic hairy roots cultivation, which reveals new aspects of synthesis, accumulation and secondary metabolites production regulation. Biosynthesis genetic regulation of significant secondary metabolites is also a promising direction. Numerous studies have demonstrated that salidroside biosynthesis depends on the expression of the TyrDC gene encoding tyrosinedecarboxylase. Consequently, understanding the molecular and genetic mechanisms opens up opportunities for its regulation and metabolic engineering. In this regard, biotechnological methods may be a priority to obtaining salidroside, rosin and their derivatives at higher or at least comparable levels with wild type or cultivated plants. Among the various biotechnological strategies used to increase the accumulation of salidroside and glycosides of cinnamic alcohol in cells and cultures of Rhodiola organs, the approach with the addition of the metabolic precursors to the nutrient medium proved to be the most effective. <i>In vitro</i> cultures, a significant increase in the production of rosin and its derivatives was observed when precursors (cinnamic alcohol, cinnamic acid and cinnamic aldehyde) were added to the medium.</p>","PeriodicalId":758,"journal":{"name":"Russian Journal of Bioorganic Chemistry","volume":"50 7","pages":"2691 - 2710"},"PeriodicalIF":1.1,"publicationDate":"2024-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142870274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Issues of the Structural Organization of Lignin and Prospects for Its Processing (A Review)","authors":"A. Yu. Kozhevnikov,&nbsp;S. L. Shestakov,&nbsp;Yu. A. Sypalova","doi":"10.1134/S1068162024070264","DOIUrl":"10.1134/S1068162024070264","url":null,"abstract":"<p>This article presents a review of the literature on studies of lignin, which is one of the most abundant biopolymers on Earth, basing on publications in the world’s most-cited sources. It is noted that, already nowadays, lignin is considered as a powerful renewable source of valuable organic raw materials, but the potential for lignin valorization is much broader than the presently exploited opportunities. Particular focus is placed on the structure and molecular organization of birch lignin; birch wood is actively used in chemical industry, while the literature on hardwood lignins is much scarcer compared to softwood lignins. The basic methods of structural investigation of such a complex and irregular polymer as lignin are discussed, and their advantages, disadvantages, and application prospects are analyzed. It is pointed out that, nowadays, the most effective methods for studying the lignin structural units are pyrolysis-gas chromatography and nuclear magnetic resonance spectroscopy. Various methods of lignin isolation from wood are described, and the influence of the isolation method on the structure of the isolated substance was evaluated. Also, the structural features of birch lignin and its distinctions from lignins of other species are shown.</p>","PeriodicalId":758,"journal":{"name":"Russian Journal of Bioorganic Chemistry","volume":"50 7","pages":"2675 - 2690"},"PeriodicalIF":1.1,"publicationDate":"2024-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142870437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Non-Agglomerated Oligonucleotide-Containing Nanocomposites Based on Titanium Dioxide Nanoparticles","authors":"M. N. Repkova,&nbsp;O. Yu. Mazurkov,&nbsp;E. I. Filippova,&nbsp;N. A. Mazurkova,&nbsp;Yu. E. Poletaeva,&nbsp;E. I. Ryabchikova,&nbsp;B. F. Zarytova,&nbsp;A. S. Levina","doi":"10.1134/S1068162024060384","DOIUrl":"10.1134/S1068162024060384","url":null,"abstract":"<p><b>Objective:</b> Stability and monodispersity are important properties of nanoparticles and nanocomposites, that ensure the reliability of their application in biological systems and the reproducibility of results. The preparation of non-agglomerated oligonucleotide-containing nanocomposites based on anatase titanium dioxide nanoparticles (Ans~ODN) is the aim of this study. <b>Methods:</b> The immobilization of oligodeoxynucleotides on TiO₂ nanoparticles was studied by dynamic light scattering and transmission electron microscopy. The antiviral activity of the synthesized samples was evaluated against VERO cells infected with herpes simplex virus type 1. <b>Results and Discussion:</b> The effect of NaCl on the agglomeration of the nanoparticles and the nanocomposites in aqueous solutions was studied. The presence of NaCl leads to agglomeration of the nanoparticles and the nanocomposites. It was shown that the nanocomposites are formed in an aqueous solution in the absence of NaCl. A comparison of the biological activities of the nanocomposites prepared in water and in saline solution was carried out on the example of inhibition of replication of the herpes simplex virus type 1 in the cell culture. The studied nanocomposite, regardless of the preparation method (in water or in 0.9% NaCl), inhibited virus replication by 4.5 orders of magnitude when used 1 day after preparation. After 10 days of storage, the activity of the sample prepared in saline solution was two orders of magnitude lower than that of the active sample prepared in water. <b>Conclusions:</b> We have developed a method for the preparation of non-agglomerated oligonucleotide-containing nanocomposites based on anatase nanoparticles and demonstrated their potential use for the study of their biological activity. Unlike nanocomposites prepared in the presence of the salt, which lose their efficacy during storage, nanocomposites that are not prone to agglomeration can be obtained in water for future use.</p>","PeriodicalId":758,"journal":{"name":"Russian Journal of Bioorganic Chemistry","volume":"50 6","pages":"2634 - 2643"},"PeriodicalIF":1.1,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142826091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}