发布求助
文献互助 智能选刊 最新文献

Cytobiologie最新文献

筛选
英文 中文
[Somatotropin, antidot against phalloidin (author's transl)]. [促生长激素，抗生殖器激素(作者译)]。
Cytobiologie Pub Date : 1978-06-01
H Schimassek, D Jeckel, W G Forssmann, H Undi
{"title":"[Somatotropin, antidot against phalloidin (author's transl)].","authors":"H Schimassek,&nbsp;D Jeckel,&nbsp;W G Forssmann,&nbsp;H Undi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>1. Somatotropin protects rats against a lethal dose of phalloidin (1.3 mg/kg). 2. Scanning electron microscopy has shown that 2 hours after phalloidin injection the liver from a somatotropin-pretreated rat is not significantly different to that from an untreated rat. Phalloidin alone caused complete destruction of the structure of the liver lobules. 3. Somatotropin does not prevent phalloidin uptake by the liver but slows down elimination. 4. The findings are discussed with respect to their therapeutic possibilities as somatropin protects rats against death also after phalloidin poisoning.</p>","PeriodicalId":75770,"journal":{"name":"Cytobiologie","volume":"17 1","pages":"84-93"},"PeriodicalIF":0.0,"publicationDate":"1978-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11890249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Electron microscopical demonstration of polysaccharides in rotifers (Aschelminthes) with the use of OsO4-ferricyanide (author's transl)]. [利用oso4 -铁氰化物对轮虫(Aschelminthes)体内多糖的电镜观察[作者简介]。
Cytobiologie Pub Date : 1978-06-01
U Schramm
{"title":"[Electron microscopical demonstration of polysaccharides in rotifers (Aschelminthes) with the use of OsO4-ferricyanide (author's transl)].","authors":"U Schramm","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Three species of rotifers were fixed with glutaraldehyde and postfixed in a OsO4-ferricyanide solution. This procedure results in staining of the sarcoplasmic reticulum (SR) and the surface coat of muscle cells as well as in staining of the surface coat of glands and nerve cells. Section-staining with leadcitrate increased the electron opacity of the precipitates, which are interpreted as polysaccharides. The partial decrease of staining of polysaccharides, which sometimes is observed, may be related to processes of function. The precipitates within the SR are considered as glycogen-beta-particles.</p>","PeriodicalId":75770,"journal":{"name":"Cytobiologie","volume":"17 1","pages":"173-81"},"PeriodicalIF":0.0,"publicationDate":"1978-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11301391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Spatio-temporal analysis of contraction dependent surface movements in Physarum polycephalum (author's transl)]. [多头绒泡菌收缩依赖表面运动的时空分析[作者译]。
Cytobiologie Pub Date : 1978-06-01
N Hülsmann, K E Wohlfarth-Bottermann
{"title":"[Spatio-temporal analysis of contraction dependent surface movements in Physarum polycephalum (author's transl)].","authors":"N Hülsmann,&nbsp;K E Wohlfarth-Bottermann","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Plasmodial veins of Physarum polycephalum were investigated by combining cinematographical and tensiometrical methods. Veins remaining on their original growing substrate show characteristic surface movements resulting from an intrinsic contraction automaticity. Radial and longitudinal components of surface movements were registered simultaneously. Both contraction activities show identical frequencies, in contrast to results derived from experiments with isolated veins. There is only one genuine frequency and therefore one has not necessarily to suppose the existence of a cooperation of two oscillating systems underlying the rhythmic contraction phenomena. The results are discussed in respect to the basis of the contraction phenomena: the cytoplasmic actomyosin fibrils of Physarum and their function in motive force generation for protoplasmic streaming.</p>","PeriodicalId":75770,"journal":{"name":"Cytobiologie","volume":"17 1","pages":"23-41"},"PeriodicalIF":0.0,"publicationDate":"1978-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11771283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Electronmicroscopical and electrophysiological investigations on polyethylene glycol induced cell fusion. 聚乙二醇诱导细胞融合的电镜和电生理研究。
Cytobiologie Pub Date : 1978-06-01 DOI: 10.18419/OPUS-1921
H. Krähling, U. Schinkewitz, A. Barker, D. Hülser
{"title":"Electronmicroscopical and electrophysiological investigations on polyethylene glycol induced cell fusion.","authors":"H. Krähling, U. Schinkewitz, A. Barker, D. Hülser","doi":"10.18419/OPUS-1921","DOIUrl":"https://doi.org/10.18419/OPUS-1921","url":null,"abstract":"Cells of monolayer cultures are fused by high concentrations of polyethylene glycol (PEG) with a molecular weight of approximately 1500. This process is independent of extracellular ca++ions. PEG changes transiently the surface membrane and leads to fusion only after replacing it by normal medium. Before the final fusion of two cells, the onset of ionic coupling via longer lasting pseudopodial contact can be measured. Only cells that are synchronous in the secretory and pseudopodial response to PEG may fuse with each other.","PeriodicalId":75770,"journal":{"name":"Cytobiologie","volume":"17 1 1","pages":"51-61"},"PeriodicalIF":0.0,"publicationDate":"1978-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67640393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 9
Distribution of adenylate cyclase among membrane fractions of rat liver. 腺苷酸环化酶在大鼠肝脏膜组织中的分布。
Cytobiologie Pub Date : 1978-06-01
W N Yunghans, D J Morré
{"title":"Distribution of adenylate cyclase among membrane fractions of rat liver.","authors":"W N Yunghans,&nbsp;D J Morré","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Adenylate cyclase activity was detected in plasma membranes, Golgi apparatus, and endoplasmic reticulum from rat liver. Adenylate cyclase activities of purified membranes were determined biochemically by two methods. In one, the synthesis of radioactive cyclic AMP from ATalpha32P was monitored. In the other, the synthesis of cyclic AMP was quantitiated using a protein which specifically binds cyclic AMP. The enzyme activity was responsive to activation by both glucagon and sodium fluoride although differences in degree of activation were noted comparing plasma membrane, Golgi apparatus, and endoplasmic reticulum. Cytochemical studies, using both whole tissue and purified cell fractions and conducted in parallel, confirmed the biochemical results. Deposition of lead phosphate, enhanced by glucagon and NaF with samples incubated with appropriate substrates, was not restricted to plasma membranes of hepatocytes but was present in intracellular membranes as well. Adenylate cyclase of rat hepatocytes appears more widely distributed among internal membranes than previously recognized.</p>","PeriodicalId":75770,"journal":{"name":"Cytobiologie","volume":"17 1","pages":"212-31"},"PeriodicalIF":0.0,"publicationDate":"1978-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11426718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Electron microscopical and autoradiographical studies on the so-called yolk-nuclei in the oocytes of Nemacheilus barbatulus (L.) and Phoxinus phoxinus (L.) (Pisces, Teleostei) (author's transl)]. [Nemacheilus barbatulus (L.)和Phoxinus Phoxinus (L.)(双鱼座，Teleostei)卵母细胞中所谓的卵黄核的电镜和自放射学研究]。
Cytobiologie Pub Date : 1978-06-01
R Riehl
{"title":"[Electron microscopical and autoradiographical studies on the so-called yolk-nuclei in the oocytes of Nemacheilus barbatulus (L.) and Phoxinus phoxinus (L.) (Pisces, Teleostei) (author's transl)].","authors":"R Riehl","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The yolk-nuclei in the oocytes of the teleosts Noemacheilus barbatulus (L.) and Phoxinus phoxinus (L.) were investigated by electron microscopy, autoradiography and ultrahistochemistry. Yolk-nuclei were only found in stage I. They consist of mitochondria, an osmiophilic material and in Noemacheilus partly of dictyosomes. It was demonstrated by marking with [3H]-uridine, that the osmiophilic material contains RNA. The osmiophilic material gets into the juxtanuclear cytoplasm. There it is surrounded by mitochondria. The oocytes of Noemacheilus and Phoxinus contain up to 40 yolk-nuclei, which are only visible by electron microscopy. The yolk-nuclei of both species dissolve in early stage II. Their components disperse in the cytoplasm.</p>","PeriodicalId":75770,"journal":{"name":"Cytobiologie","volume":"17 1","pages":"137-45"},"PeriodicalIF":0.0,"publicationDate":"1978-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11890417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Fine structure of cytoplasmic crystal in Amoeba proteus. 变形变形虫细胞质晶体的精细结构。
Cytobiologie Pub Date : 1978-06-01
W Stockem
{"title":"Fine structure of cytoplasmic crystal in Amoeba proteus.","authors":"W Stockem","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":75770,"journal":{"name":"Cytobiologie","volume":"17 1","pages":"301-6"},"PeriodicalIF":0.0,"publicationDate":"1978-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11890424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Transformation of skin fibroblast cells of a cystinotic patient by simian virus 40: evidence for an establishment of a permanent cell clone which retains the original metabolism defect. 猴病毒40对胱氨酸病患者皮肤成纤维细胞的转化:建立保留原有代谢缺陷的永久性细胞克隆的证据。
Cytobiologie Pub Date : 1978-06-01
G Darai, E Harms, R M Flügel, R Braum, K Munk
{"title":"Transformation of skin fibroblast cells of a cystinotic patient by simian virus 40: evidence for an establishment of a permanent cell clone which retains the original metabolism defect.","authors":"G Darai,&nbsp;E Harms,&nbsp;R M Flügel,&nbsp;R Braum,&nbsp;K Munk","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Human skin fibroblast cells derived from a juvenile patient with nephropathic cystinosis were transformed by simian virus 40. Transformed cell clones were isolated and established in tissue culture. In comparison to the parental cystinotic cells, the newly isolated, transformed cell clones had a higher plating efficiency, a modal chromosome number of 68, grew in soft agar, and showed a nuclear immunofluorescence typical for SV 40-specific tumor (T) antigen. The content of intracellular, unbound cystine in the transformed cell clone was of the same level (6.1 nmol 1/2 cystine/mg protein) as in the parental cystinotic cells (7.4 nmol). Control cells (SV 80 and WI-38) contained normal levels of cystine (0.31 and 0.47 nmol 1/2 cystine/mg protein). The growth characteristics make the transformed cystinotic cell clone suitable for large scale preparation of cellular constituents, i.e. lysosomes which seem to be affected in cystinotic patients.</p>","PeriodicalId":75770,"journal":{"name":"Cytobiologie","volume":"17 1","pages":"42-50"},"PeriodicalIF":0.0,"publicationDate":"1978-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11426719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of cytochalasin A on the morphology of plasmodia and sclerotia of Physarum polycephalum. 细胞松弛素A对多头绒泡菌疟原虫和菌核形态的影响。
Cytobiologie Pub Date : 1978-06-01
S D Mante, M Flashner, S W Tanenbaum
{"title":"Effects of cytochalasin A on the morphology of plasmodia and sclerotia of Physarum polycephalum.","authors":"S D Mante,&nbsp;M Flashner,&nbsp;S W Tanenbaum","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Cytochalasin A (CA) at 1.6 X 10(-5)M and lower concentrations produced disruptive effects upon plasmodia, sclerotia, and spherule forms of Physarum polycephalum. CA effects upon either micro- or macroplasmodia included: cytoplasmic condensation, plasmodial contraction, and scission at the plasma membrane. The latter manifestation was most dramatically observed by scanning electron microscopy. Electron microscopy of drug-treated palsmodia confirmed the above phase contrast light-microscopic results and revealed, in addition, vacuolar enlargement, decreased membrane invaginations, and the presence of condensed particles within the plasmodium and at the plasma membrane. These results of drug action were not reversed by washing of exposed plasmodia. Germination of the slcerotial and spherule forms of Physarum was CA-delayed by 12 and 96 hours, respectively. Approximately 10% of drug-treated sclerotia were found to have been burst apart. These dramatic CA effects were nullified by preincubation of the drug either with L-cysteine or with beta-mercaptoethanol; however, iodoacetamide (10(-6)M) gave no such morphologic consequences. Cytochalasins B or D at comparable concentrations were without activity. It is concluded therefore that CA effects upon the myoxomycete reflect specific acceptor responses.</p>","PeriodicalId":75770,"journal":{"name":"Cytobiologie","volume":"17 1","pages":"10-22"},"PeriodicalIF":0.0,"publicationDate":"1978-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11890415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Cytological phenomena studied in Vicia faba, in relation to Pelc's hypothesis of a "metabolic" DNA (author's transl)]. [蚕豆的细胞学现象研究，与Pelc的“代谢”DNA假说有关[作者译]。
Cytobiologie Pub Date : 1978-06-01
W Scheuermann
{"title":"[Cytological phenomena studied in Vicia faba, in relation to Pelc's hypothesis of a \"metabolic\" DNA (author's transl)].","authors":"W Scheuermann","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":75770,"journal":{"name":"Cytobiologie","volume":"17 1","pages":"232-45"},"PeriodicalIF":0.0,"publicationDate":"1978-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11890422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
首页 上一页 下一页 尾页
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整，为了账户安全，请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们：info@booksci.cn Book学术提供免费学术资源搜索服务，方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号：481959085
Book学术官方微信