{"title":"[Effects of phalloidin in liver cells: binding, morphological changes, and elimination (author's transl)].","authors":"H Schimassek, D Jeckel","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>1. The uptake, binding and elimination of phalloidin in liver is compared in adult (180 to 240 g) and \"baby\" (17 to 19 days old) rats in vivo and in vitro. 2. In both groups there is no relation between the concentration of the poison in the liver and the toxicity. 3. Although baby rats show a significantly higher tolerance against phalloidin than the adult animals, the concentration of the poison in the liver of baby rats is higher, and the elimination is significantly slower than in adult rats. 4. The very tight binding and concentration of phalloidin in the liver is explained by an extremely low dissociation constant. 5. Furthermore, the morphological differences between the poisoning of the liver cells in the entire organ and of isolated liver cells are discussed.</p>","PeriodicalId":75770,"journal":{"name":"Cytobiologie","volume":"17 1","pages":"73-83"},"PeriodicalIF":0.0,"publicationDate":"1978-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11890248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Electronmicroscopical and electrophysiological investigations on polyethylene glycol induced cell fusion.","authors":"H Krähling, U Schinkewitz, A Barker, D F Hülser","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Cells of monolayer cultures are fused by high concentrations of polyethylene glycol (PEG) with a molecular weight of approximately 1500. This process is independent of extracellular ca++ions. PEG changes transiently the surface membrane and leads to fusion only after replacing it by normal medium. Before the final fusion of two cells, the onset of ionic coupling via longer lasting pseudopodial contact can be measured. Only cells that are synchronous in the secretory and pseudopodial response to PEG may fuse with each other.</p>","PeriodicalId":75770,"journal":{"name":"Cytobiologie","volume":"17 1","pages":"51-61"},"PeriodicalIF":0.0,"publicationDate":"1978-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11890425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Biological horizons in molecular microscopy.","authors":"W Baumeister","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The purpose envisaged in this report is not to provide a comprehensive monography but rather to give a survey, especially for biologists, of the state of the art and of current research trends in molecular microscopy. Following a brief discussion of the obvious discrepancy between instrumental capabilities and the limits of biologically significant information, a definition of the diversifying field is attempted. Four main topics are discussed. First, recent progress in the field of \"low noise\" specimen supports is reviewed. It is emphasized that a minimum background structure is an important but not the sole criterion for a satisfactory support. It is the ability to adsorb molecules in a predeterminable and orderly fashion which will attract wider attention in the future; positional and orientational order figure as crucial points in the strategem of low dose microscopy. Second, the problem of achieving adequate contrast without the expense of an unfaithful representation of molecular structures is discussed. Contrast is a problem of optimum imaging modes as well as of preparatory techniques. The third topic of discussion is specimen dehydration. Several avenues to circumvent or at least to alleviate dehydration artifacts are outlined. The last chapter focusses on the most fundamental problem in molecular microscopy:radiation damage. A brief synopsis of the physical and physico-chemical processes involved in damaging interactions is given and an attempt is made to tesselate the true picture of radiation damage to lipids and proteins. This might serve as a guidance in assessing the degree of structural fidelity to be expected for a given electron dose. Possibilities to overcome the radiation damage problem are adumbrated.</p>","PeriodicalId":75770,"journal":{"name":"Cytobiologie","volume":"17 1","pages":"246-97"},"PeriodicalIF":0.0,"publicationDate":"1978-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11301392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The structure of a periodic cell wall component (HPI-layer of Micrococcus radiodurans).","authors":"O Kübler, W Baumeister","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The hexagonally packed interlayer (HPI-layer) from Micrococcus radiodurans cell walls has been studied by electron microscopy and subsequent digital image processing. The most prominent feature in the average images is a \"complex\" shaped like a \"toothed wheel\", which is perforated by a central pore and interconnected by fine spokes. This basic structural element is tentatively interpreted to represent the bulk of HPI-layer protein, intercalated by the other constituents: lipids, carotenoids and carbohydrates. It is suggested that the \"toothed wheel\" structure is a quite common element of periodic bacterial surface layers and that the different spacings observed with various species are due to variable amounts of intercalating material.</p>","PeriodicalId":75770,"journal":{"name":"Cytobiologie","volume":"17 1","pages":"1-9"},"PeriodicalIF":0.0,"publicationDate":"1978-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11890414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Ultrastructure of the frontal cap of monotactic forms of Amoeba proteus.","authors":"B Hrebenda, L Grebecka","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The frontal cap of the monotactic form of Amoeba proteus is separated from other cell components by a continuous structure defined as the \"membrane-like envelope\" (MLE). It originates from the membranes of cytoplasmic vesicles and vacuoles. The border zone between the cap and the cytoplasm is strongly vacuolized. Structural differences between frontal caps, depending on the degree of their development, indicate that the growing cap gradually fills up the whole tip of an advancing pseudopodium, and at the front it reduces the cortical layer in the interstice between the MLE and the outer cell membrane, up to its eventual disrupture. This is probably the efficient cause of the specific morphological and motory pattern of monotactic amoebae. These results and conclusions are supported by an ultrastructural analysis of the artificial frontal caps obtained by injecting oil droplets into polytactic cells, a procedure transforming polytactic forms into forms morphodynamically analogous to the natural monotactic amoebae.</p>","PeriodicalId":75770,"journal":{"name":"Cytobiologie","volume":"17 1","pages":"62-72"},"PeriodicalIF":0.0,"publicationDate":"1978-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11890247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Differential growth of sister nuclei in binucleate microspores of Tradescantia paludosa.","authors":"R L White, D Davidson","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Binucleate microspores were induced in Tradescantia paludosa with heat shocks. Nuclear and cell volumes were determined from early G1 to G2. Volumes of sister nuceli, present in a single cell, were rarely the same. It is suggested that, at least in part, nuclear volume is controlled by factors that are 1) present in nuclei, 2) not distributed equally at anaphase, and 3) not rapidly exchanged.</p>","PeriodicalId":75770,"journal":{"name":"Cytobiologie","volume":"17 1","pages":"159-64"},"PeriodicalIF":0.0,"publicationDate":"1978-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11890419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Architecture and cell structures in the distal nephron of the rat kidney.","authors":"M L Crayen, W Thoenes","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Using semi-thin sections of marked tubules of the rat kidney, the distal part of the nephron is represented in toto for the first time and reconstructed in its course from the thick part of HENLE's loop to its junction with another collecting tubule. The shape of the model accords well with observations in vivo. On the basis of observations with light and electron microscopy, four types of cells are differentiated: Type 1 (Mittelstück) cell; type 2 (intermediate) cell; type 3 (light) cell and type 4 (intercalated) cell. According to the distribution of these different types of cells, the distal nephron is divided in the following way: Pars recta and pars convoluta of the distal nephron consist of type 1 (Mittelstück) cells; pars arcuata and pars recta of the cortical collecting duct exhibit type 3 (light) and type 4 (intercalated) cells; in the connecting portion of the distal tubule all four types are represented, type 2 (intermediate) and type 4 (intercalated) being the most frequent. The demarcation of the connecting portion according to our definition points to a dynamic relationship of the cell structures of the distal nephron, which gains clarity when conditions are experimentally varied. Hence the question for a morphological proof of where exactly the two nephrogenic blastemata join gives place to the more important question of what these cell structures and their variability mean as signs of functional adaptation-reactions.--The existence of the connecting portion, contested until now, is affirmed; a cytological characterization of it is given and a definition is recommended.</p>","PeriodicalId":75770,"journal":{"name":"Cytobiologie","volume":"17 1","pages":"197-211"},"PeriodicalIF":0.0,"publicationDate":"1978-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11890421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A model for microtubular rigidity.","authors":"J Bereiter-Hahn","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":75770,"journal":{"name":"Cytobiologie","volume":"17 1","pages":"298-300"},"PeriodicalIF":0.0,"publicationDate":"1978-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11890423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Studies on nucleoli of pigeon erythroid cells.","authors":"K Smetana, Z Likovsky","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In order to provide more information on nucleolar changes occurring during cell differentiation and maturation, pigeon erythroid cells have been studied by means of a simple light microscopic cytochemical procedure for the demonstration of the RNA containing structures and with conventional transmission electron microscopy. Nucleoli with more or less distinct nucleolonemata present in early erythroblasts were replaced by ring-shaped nucleoli and, finally, by micronucleoli in more mature erythroid cells. In contrast to the previously studied chick embryos, chickens and hens which possess micronucleoli in almost all mature erythrocytes, mature pigeon erythrocytes are mostly without any nucleoli. The ultrastructural organization of nucleoli with more or less distinct nucleolonemata and ring-shaped nucleoli do not show differences, in comparison with such forms of nucleoli in other cells. Micronucleoli in pigeon erythroblasts are characterized by degranulation, suggesting the inhibition of the formation of the nucleolar granular components. Some nucleoli show a segregation of the nucleolar components, indicating the inactivation of the nucleolar RNA polymerase, and occasionally chromatin retraction from the nucleolar body, suggesting the loss of the template. In mature erythrocytes, micronucleoli consist mainly of fibrillar components, and the perinucleolar chromatin is partially retracted from the nucleolar body of such nucleoli.</p>","PeriodicalId":75770,"journal":{"name":"Cytobiologie","volume":"17 1","pages":"146-58"},"PeriodicalIF":0.0,"publicationDate":"1978-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11890418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Ultrastructural organization of the ocelloide of Nematodinium. Phylogenetic aspect of the evolution of Warnowiidae Lindemann dinoflagellates photoreceptor (author's transl)].","authors":"C Greuet","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In this study, more details of the different parts of the \"ocelloide\" of Nematodinium are given. The \"hyalosome\" which has a radial symmetry is made of endoplasmic reticulum, except the mitochondrial layer. There is only one constrictor ring and only a beginning of the periocellar gallery. The basal sheet on which the crystalline lens lies is a local differentiation of the cortical layer which is observed on the ceiling of the ocellar camera. This camera is related to the intercingular sulcus. There is also an ocellar fiber. The \"melanosome\" which is made of a double membrane and of many couples of thylakoids is flattened dorso-ventrally. The structure of the retina is similar to that observed in the other Warnowiidae. The pigmented ring is rather well developed and is in continuity with the upper part of the retina. Owing to the simpler organization of its ocelloide, Nematodinium is situated before the genera Warnowia and Erythropsidinium in the evolutionary series.</p>","PeriodicalId":75770,"journal":{"name":"Cytobiologie","volume":"17 1","pages":"114-36"},"PeriodicalIF":0.0,"publicationDate":"1978-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11771280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}