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The intermediate-sized filaments in rat kangaroo PtK2 cells. I. Morphology in situ. 大鼠袋鼠PtK2细胞的中等大小纤维。1 .原位形态学。
Cytobiologie Pub Date : 1978-08-01
W W Franke, C Grund, M Osborn, K Weber
{"title":"The intermediate-sized filaments in rat kangaroo PtK2 cells. I. Morphology in situ.","authors":"W W Franke,&nbsp;C Grund,&nbsp;M Osborn,&nbsp;K Weber","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The system of the intermediate-sized filaments (IF) of rat kangaroo PtK2 cells which can be specifically demonstrated by immunofluorescence microscopy using certain rabbit autoantibodies and guinea pig antibodies against bovine hoof prekeratin has been studied by electron microscopy. The characteristic ornamental, curved arrays of this system are shown after fixation in situ in both thin sections and whole-cell-preparations to represent bundles of 6 to 11 nm thick filaments extending through the whole cytoplasm, although in some cells they appear to be enriched in the perinuclear region. While many individual IF are recognized in the cytoplasm the tendency of such filaments to aggregate laterally into bundles is one of their prominent features. Among such bundle formations one form that consists of tightly packed IF cemented together in a dense osmiophilic matrix is especially conspicious. The appearance and mode of arrangement of the IF is not significantly altered in cells treated with colcemid and/or cytochalasin B. Spatial relationships of IF with microfilament-containing cables and microtubules as well as with membranous structures are also described. IF are heterogeneous in width and reveal an unstained, apparently hollow core, indicative of a tubular organization. Many IF show small, sometimes periodically arranged lateral projections which seem to be involved in IF cross-linking. Associations with polyribosomes are common. The changes in the IF system during mitosis have also been examined. The structural details of the IF as well as their possible role as cytoskeletal elements involved in the control of cell shape and cytoplasmic architecture are discussed in relation to data on various intermediate-sized filaments from other cell types. The close similarity of the IF of PtK2 cells to aggregates of prekeratin filaments is emphasized. It is suggested that PtK2 cells represent an epithelial cell line growing in a state of balanced semi-keratinization.</p>","PeriodicalId":75770,"journal":{"name":"Cytobiologie","volume":"17 2","pages":"365-91"},"PeriodicalIF":0.0,"publicationDate":"1978-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11567556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Specific visualization of the distribution of the calcium dependent regulatory protein of cyclic nucleotide phosphodiesterase (modulator protein) in tissue culture cells by immunofluorescence microscopy: mitosis and intercellular bridge. 组织培养细胞中环核苷酸磷酸二酯酶钙依赖性调节蛋白(调节蛋白)分布的免疫荧光显微镜特异性可视化:有丝分裂和细胞间桥。
Cytobiologie Pub Date : 1978-08-01
B Andersen, M Osborn, K Weber
{"title":"Specific visualization of the distribution of the calcium dependent regulatory protein of cyclic nucleotide phosphodiesterase (modulator protein) in tissue culture cells by immunofluorescence microscopy: mitosis and intercellular bridge.","authors":"B Andersen,&nbsp;M Osborn,&nbsp;K Weber","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Monospecific antibodies against the homogeneous Ca++ dependent regulatory protein of cyclic nucleotide phosphodiesterase (CDR protein) from bovine brain were used in indirect immunofluorescence microscopy to visualize the cytoplasmic organization of this key regulatory protein in growing tissue culture cells. Although cells during interphase reveal only a weak general cytoplasmic fluorescence, a dramatic reorganization of CDR protein occurs with the onset of mitosis. Throughout the different mitotic stages CDR protein is strongly concentrated in the two polar parts of each half spindle. After completion of telophase (CDR protein appears at both cytoplasmic ends of the intercellular bridge which still connects the two daughter cells. Parallel use of monospecific antibodies against CDR protein and tubulin emphasizes the spatial restriction in the localization of CDR protein during mitosis and early G1 phase of the cell cycle.</p>","PeriodicalId":75770,"journal":{"name":"Cytobiologie","volume":"17 2","pages":"354-64"},"PeriodicalIF":0.0,"publicationDate":"1978-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11567555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Response to light-shade difference in anucleate and polynucleate specimens of Amoeba proteus. 变形变形虫无核和多核标本对光荫差异的响应。
Cytobiologie Pub Date : 1978-08-01
A Grebecki, L V Kalinina, L Grebecka
{"title":"Response to light-shade difference in anucleate and polynucleate specimens of Amoeba proteus.","authors":"A Grebecki,&nbsp;L V Kalinina,&nbsp;L Grebecka","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The enucleated specimens of Amoeba proteus, the anucleate fragments, and the polynucleate individuals which all are capable of cortical contraction but not of locomotion, may be reactivated by the light-shade difference established across their body. Individual cells or fragments migrate toward the shade. The motory polarity and coordinated movement disappear immediately after cessation of the stimulus. The results are interpreted according to the earlier hypothesis that the necessary to maintain the motory polarity of amoebae. It is suggested that the anucleate and polynucleate specimens are incapable of coordinated movements when non-stimulated, because of a deficiency or an excess, respectively, of the regulatory relaxing factor secreted by the nucleus of Amoeba proteus.</p>","PeriodicalId":75770,"journal":{"name":"Cytobiologie","volume":"17 2","pages":"343-53"},"PeriodicalIF":0.0,"publicationDate":"1978-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11890252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
X-ray microanalysis of calcium-dependent deposits at the plasma membrane of Acanthamoeba castellanii. 棘阿米巴(棘阿米巴)质膜钙依赖性沉积物的x射线显微分析。
Cytobiologie Pub Date : 1978-08-01
A Sobota, A Przelecka, A G Janossy
{"title":"X-ray microanalysis of calcium-dependent deposits at the plasma membrane of Acanthamoeba castellanii.","authors":"A Sobota,&nbsp;A Przelecka,&nbsp;A G Janossy","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":75770,"journal":{"name":"Cytobiologie","volume":"17 2","pages":"464-9"},"PeriodicalIF":0.0,"publicationDate":"1978-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11890128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Plasmodium of Physarum polycephalum as a synchronous contractile system. 作为一个同步收缩系统的多头绒泡菌的疟原虫。
Cytobiologie Pub Date : 1978-08-01
A Grebecki, M Cieślawska
{"title":"Plasmodium of Physarum polycephalum as a synchronous contractile system.","authors":"A Grebecki,&nbsp;M Cieślawska","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The contractile activity of veins and the rhythmicity of the frontal progress were photometrically recorded from cine-film, at numerous points across the whole plasmodium. Graphic analysis of the obtained curves demonstrates the existence of common contraction rhythm over the entire network, coincident to the expansion rhythm of the advancing front. It is suggested that the plasmodium of Physarum polycephalum represents an imperfectly synchronized monorhythmic contractile system.</p>","PeriodicalId":75770,"journal":{"name":"Cytobiologie","volume":"17 2","pages":"335-42"},"PeriodicalIF":0.0,"publicationDate":"1978-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11890251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Fine structure of Sertoli cells in the rat testis after hypophysectomy, testosterone treatment and re-involution. 垂体切除术、睾酮治疗和再退化后大鼠睾丸内支持细胞的精细结构。
Cytobiologie Pub Date : 1978-08-01
G Aumüller, A Schiller
{"title":"Fine structure of Sertoli cells in the rat testis after hypophysectomy, testosterone treatment and re-involution.","authors":"G Aumüller,&nbsp;A Schiller","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Using thin sections and freeze-etch replicas the fine structure of the Sertoli cells of the rat testis was investigated after hypophysectomy, testosterone treatment and re-involution. 41 days after hypophysectomy the Sertoli cells contain numerous dense bodies and remnants of degenerating spermatocytes and spermatids. The Sertoli cell junctions are most prominent. The membranes of neighbouring cells are folded into several layers. Freeze-fracture replicas reveal a normal arrangement of Sertoli cell tight junctions with linear array of membrane particles preferentially on the B-face and complementary grooves on the A-face. The geometric pattern of the ridges is varying with respect to the basal, intermediate and apical portions of the lateral Sertoli cell membranes. Since no major changes of the size, distribution and localization of the Sertoli cell junctions were observed in the different experimental groups these junctions, once formed, are inferred to be independent from hypophyseal hormones.</p>","PeriodicalId":75770,"journal":{"name":"Cytobiologie","volume":"17 2","pages":"453-63"},"PeriodicalIF":0.0,"publicationDate":"1978-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11890127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Nature of the phosphotungstic acid-chromic acid (PACP) stain for plasma membranes of plants and mammalian sperm. 植物和哺乳动物精子质膜的磷钨酸-色酸（PACP）染色剂的性质。
Cytobiologie Pub Date : 1978-06-01
W N Yunghans, J E Clark, D J Morré, E D Clegg
{"title":"Nature of the phosphotungstic acid-chromic acid (PACP) stain for plasma membranes of plants and mammalian sperm.","authors":"W N Yunghans, J E Clark, D J Morré, E D Clegg","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The selective staining of plasma membranes of plants and porcine spermatozoa given by a mixture consisting of 1% phosphotungstic acid in 10% chromic acid (PACP) applied following periodic acid destaining of glutaraldehyde-osmium tetroxide-fixed electron microscope sections is reduced or eliminated by prior extraction of the tissues with lipid solvents, including ethanol. The ethanol-soluble fraction of sperm contains constituents which restore the PACP-staining reaction when added to ethanol-extracted and lyophilized sperm. Analysis of the ethanol extracts by thin layer chromatography revealed two major components which reacted with both phosphotungstic acid (PTA) and alpha-naphthol detection reagents. These PTA-positive constituents were concentrated in plasma membranes of sperm; components with similar mobilities were found in fractions of plasma membranes from plants. Addition of the PTA-positive constituents from either sperm or plants to extracted and lyophilized sperm restored the PACP staining. The findings are interpreted to mean that one or more low molecular weight constituents (saccharides or glycolipids), rather than glycoproteins, concentrated in plaslma membranes are responsible for the unique PACP staining in both plants and porcine sperm.</p>","PeriodicalId":75770,"journal":{"name":"Cytobiologie","volume":"17 1","pages":"165-72"},"PeriodicalIF":0.0,"publicationDate":"1978-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11301564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Penetration and entrapment of large particles in erythrocytes by electrical breakdown techniques. 电击穿技术在红细胞中穿透和捕获大颗粒。
Cytobiologie Pub Date : 1978-06-01
J Vienken, E Jeltsch, U Zimmermann
{"title":"Penetration and entrapment of large particles in erythrocytes by electrical breakdown techniques.","authors":"J Vienken,&nbsp;E Jeltsch,&nbsp;U Zimmermann","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Human erythrocytes suspended in isotonic solutions were subjected to haemolysis by application of an electric field pulse to the cell suspension. The field strengths used were 12 and 16kV/cm, respectively; the pulse duration 40 microseconds. The lysed cells showed resealing properties. The permeability change of the membrane generated by the field pulse and by the subsequent osmotic processes were large enough to facilitate the penetration and entrapment of ferritin and Latex particles (diameter: 0.091 and 0.176 micron, respectively) as revealed by electron microscopy. Correct identification of the Latex particles in the electron-micrographs indicated that LOYTER et al. [J. Cell Biol. 66, 292 (1975)], who recently demonstrated the entrapment of Latex spheres in erythrocytes prepared by osmotic haemolysis mistook electron-dense bodies probably consisting of denaturated protein for Latex particles. Under conditions of osmotic haemolysis, carried out according to BODEMANN and PASSOW, particles could only occasionally be detected within the membrane itself and never within the cell interior, suggesting that the electrical haemolysis method is much more effective in the generation of large holes in the membrane.</p>","PeriodicalId":75770,"journal":{"name":"Cytobiologie","volume":"17 1","pages":"182-96"},"PeriodicalIF":0.0,"publicationDate":"1978-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11890420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Membranous sacs associated with cilia of Paramecium. 草履虫纤毛相关的膜囊。
Cytobiologie Pub Date : 1978-06-01
D J Patterson
{"title":"Membranous sacs associated with cilia of Paramecium.","authors":"D J Patterson","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A membranous system associated with the cortical cilia of Paramecium putrinum is described. A sac lies below the cortical kinetosomes and extensions from it pass along the kinetodesmal fibre to lie near, or make contact with, the parsomal sac and also pass deep into the cytoplasm to pass close to a mitochondrion. Because of its widespread occurrence in this and other species it is suggested that the system plays a physiologically active role.</p>","PeriodicalId":75770,"journal":{"name":"Cytobiologie","volume":"17 1","pages":"107-13"},"PeriodicalIF":0.0,"publicationDate":"1978-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11890416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Selective extraction of cytoplasmic action-containing filaments with DNA-ase I. dna酶ⅰ选择性提取细胞质含活性纤维。
Cytobiologie Pub Date : 1978-06-01
T R Raju, M Steward, I K Buckley
{"title":"Selective extraction of cytoplasmic action-containing filaments with DNA-ase I.","authors":"T R Raju,&nbsp;M Steward,&nbsp;I K Buckley","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":75770,"journal":{"name":"Cytobiologie","volume":"17 1","pages":"307-11"},"PeriodicalIF":0.0,"publicationDate":"1978-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11771285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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