Cytobiologie最新文献

Coumarin-induced disturbances of morphological development and cell wall formation in Trichophyton mentagrophytes. 香豆素诱导的毛藓植物形态发育和细胞壁形成的干扰。

Cytobiologie Pub Date : 1979-02-01

G Dall'Olio, G L Vannini

引用次数: 0

[The fine structure of myotendinous and myo-epithelial junctions in the guinea pig tongue (author's transl)]. [豚鼠舌肌腱和肌上皮连接的精细结构(作者译)]。

Cytobiologie Pub Date : 1979-02-01

U Demmel, U Schewe, P Böck, K Gorgas

引用次数: 0

The size and number of intramembrane particles in cells of the photosynthetic bacterium Rhodopseudomonas capsulata studied by freeze-fracture electron microscopy. 冻裂电镜研究了光合细菌荚膜红假单胞菌细胞膜内颗粒的大小和数量。

Cytobiologie Pub Date : 1979-02-01

J Golecki, G Drews, R Bühler

{"title":"The size and number of intramembrane particles in cells of the photosynthetic bacterium Rhodopseudomonas capsulata studied by freeze-fracture electron microscopy.","authors":"J Golecki, G Drews, R Bühler","doi":"","DOIUrl":"","url":null,"abstract":"By freeze-fracture electron microscopy, particles have been observed on the protoplasmic leaflet (PF face) of cytoplasmic and intracytoplasmic membranes of the photosynthetic bacterium Rhodopseudomonas capsulata. The particles are present under all culture conditions of chemotrophically and phototrophically grown cells. However, the number of particles per microM2 increased significantly when the formation of the photosynthetic apparatus in the membrane is induced. Intracytoplasmic membranes, where the bulk of photosynthetic activity is localized, always have a higher density of particles than cytoplasmic membranes. Under all conditions particles with a diameter of 9.5 nm dominate. The frequency of particles with diameters greater or smaller than 9.5 nm changed with culture conditions. A comparison of biochemical and electron microscopic data have lead us to the conclusion that the particles, formed under conditions which allow the synthesis of the photosynthetic apparatus, are composed of photochemical reaction centers and antenna light-harvesting bacteriochlorophyll I (B 875)-protein complexes. The total molecular weight of these particles is calculated to be 500,000.","PeriodicalId":75770,"journal":{"name":"Cytobiologie","volume":"18 3","pages":"381-9"},"PeriodicalIF":0.0,"publicationDate":"1979-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11635393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dependence of intracellular alkali-ion concentrations of 3T3 and SV 40-3T3 cells on growth density. 3T3和SV 40-3T3细胞胞内碱离子浓度对生长密度的影响。

Cytobiologie Pub Date : 1979-02-01

M Ernst, G Adam

{"title":"Dependence of intracellular alkali-ion concentrations of 3T3 and SV 40-3T3 cells on growth density.","authors":"M Ernst, G Adam","doi":"","DOIUrl":"","url":null,"abstract":"Intracellular contents of potassium and of sodium are determined for 3T3 and SV 40-3T3 cells in dependence of growth density. In parallel, total cell volume and volume of intracellular water is determined for these cells suspended in physiological buffer. Intracellular potassium concentration thus evaluated for suspended 3T3 cells exhibits a sharp decrease at cellular growth densities which lead to density dependent inhibition of cell proliferation. In the case of SV 40-3T3 cells, this drop of potassium concentration with increasing cellular growth density is not observed, which correlates well with the absence of cell density dependent inhibition of cell growth in the transformed cell line. These results support the notion that processes of stimulation of quiescent 3T3 cells or of cell density dependent inhibition of their proliferation are mediated by processes including changes of potassium transport characteristics leading to increase or decrease respectively of their intracellular potassium concentration. Furthermore, these and other results suggest, that a difference between normal and transformed cells most relevant to their different proliferation behaviour might reside in different transport characteristics for potassium of the plasma membranes of these cells.","PeriodicalId":75770,"journal":{"name":"Cytobiologie","volume":"18 3","pages":"450-9"},"PeriodicalIF":0.0,"publicationDate":"1979-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11434718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Visualization of the interphase chromosomes of Ornithogalum virens and Muntiacus muntjak. 鸟类和芒提库斯间期染色体的可视化。

Cytobiologie Pub Date : 1979-02-01

D B Brown, S M Stack, J B Mitchell, J S Bedford

{"title":"Visualization of the interphase chromosomes of Ornithogalum virens and Muntiacus muntjak.","authors":"D B Brown, S M Stack, J B Mitchell, J S Bedford","doi":"","DOIUrl":"","url":null,"abstract":"A technique for visualizing \"interphase chromosomes\" was applied to nuclei of the angio-spermous plant, Ornithogalum virens (2 n = 6), and the male mammal, Muntiacus munjak (2 n = 7), in an attempt to correlate the numbers of \"chromosomes\" visible during interphase with the respective diploid chromosome numbers. The alterations in chromosome structure observed during G1, S, and G2 periods were comparable to those previously reported in Allium cepa and Chinese hamster (CHO line) cells [33], but for technical reasons it was only possible to make accurate counts of interphase chromosomes in the G1 nuclei of O. virens. In addition, from our observations of interphase chromosomes that were pulse-labelled with tritiated thymidine and a parallel study of premature chromosome condensation (PCC) using pulse-labelled M.muntjak cells, we conclude that, although chromatin decondensation may be required for DNA synthesis, extreme chromatin decondensation can occur in the absence of DNA synthesis. Generally a morphological description of alterations in chromatin during interphase only roughly parallels the G1, S, and G2 phases defined by autoradiography following incorporation of tritiated thymidine. We suggest that both methods are valid through different ways of describing interphase.","PeriodicalId":75770,"journal":{"name":"Cytobiologie","volume":"18 3","pages":"398-412"},"PeriodicalIF":0.0,"publicationDate":"1979-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11635395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lipoprotein secretion by rat liver Golgi apparatus. Lipoprotein particles and lipase activity. 大鼠肝脏高尔基体脂蛋白分泌。脂蛋白颗粒与脂肪酶活性。

Cytobiologie Pub Date : 1979-02-01

K A Hess, D J Morré, W D Merritt

{"title":"Lipoprotein secretion by rat liver Golgi apparatus. Lipoprotein particles and lipase activity.","authors":"K A Hess, D J Morré, W D Merritt","doi":"","DOIUrl":"","url":null,"abstract":"Lipoprotein particles of the size range of very low density lipoproteins in smooth endoplasmic reticulum, peripheral elements of the Golgi apparatus, and secretory vesicles of the immature Golgi apparatus face are 55 to 80 nm in diameter. Particles in mature secretory vesicles are smaller (45 nm). Concomitant with the change in particle size, the lumina of mature vesicles increase in electron density. A technique to fractionate immature and mature secretory vesicles was based on precipitation of a cupric-ferrocyanide complex (Hatchett's brown) through the action of a NADH-ferricyanide oxido-reductase resistant to glutaraldehyde which is characteristic of the membranes of mature secretory vesicles and of the plasma membrane of liver. Mature secretory vesicle fractions so isolated were enriched in cholesterol and depleted in triglycerides relative to immature vesicles on a phospholipid basis. Lipase activity was present in secretory vesicle fractions of the Golgi apparatus as shown by biochemical analysis and by cytochemistry. Cytochemical studies showed lipase to be present in both mature and immature vesicles but most evident in immature vesicles. The findings suggest that some very low density lipoprotein particles are converted to particles of smaller diameter during transit through Golgi apparatus. A lipase-mediated hydrolysis of triglycerides may relate to the transformation.","PeriodicalId":75770,"journal":{"name":"Cytobiologie","volume":"18 3","pages":"431-49"},"PeriodicalIF":0.0,"publicationDate":"1979-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11434717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microtubule reassembly-interactions between microtubules and pore complexes, endoplasmic reticulum and 100 A filaments in tumour cells in vitro. 微管重组:肿瘤细胞中微管与孔复合物、内质网和100a丝的相互作用。

Cytobiologie Pub Date : 1978-12-01

J Chemnitz, K Salmberg

引用次数: 0

Cytochemical demonstration of glutaraldehyde-resistant NADH-ferricyanide oxido-reductase activities in rat-liver plasma membranes and Golgi apparatus. 大鼠肝质膜和高尔基体抗戊二醛nadh -铁氰化物氧化还原酶活性的细胞化学证明。

Cytobiologie Pub Date : 1978-12-01

D J Morré, E L Vigil, C Frantz, H Goldenberg, F L Crane

{"title":"Cytochemical demonstration of glutaraldehyde-resistant NADH-ferricyanide oxido-reductase activities in rat-liver plasma membranes and Golgi apparatus.","authors":"D J Morré, E L Vigil, C Frantz, H Goldenberg, F L Crane","doi":"","DOIUrl":"","url":null,"abstract":"NADH-ferricyanide reductase activity was demonstrated in rat liver endomembranes by cytochemical procedures. The activity observed in plasma membrane and mature portions of the Golgi apparatus resisted fixation in 0.1% glutaraldehyde, a characteristic which permitted differentiation of the NADH-ferricyanide reductase of plasma membranes and mature Golgi apparatus elements from those of mitochondria, microbodies, endoplasmic reticulum and nuclear envelope. With the latter membranes, activity could be demonstrated only with unfixed material or following brief glutaraldehyde fixation and was greatest with broken cells or isolated fractions due to problems of penetration of reagents. Biochemical studies paralleled cytochemical findings with respect to glutaraldehyde fixation and sensitivity to other metabolic inhibitors. The findings provide evidence that a NADH-ferricyanide reductase may be among the membrane constituents conserved and/or modified during flow differentiation of membranes. The basis for a method to evaluate plasma membrane contamination of endoplasmic reticulum fractions and to differentiate among mature and immature secretory vesicles of the Golgi apparatus is also indicated.","PeriodicalId":75770,"journal":{"name":"Cytobiologie","volume":"18 2","pages":"213-30"},"PeriodicalIF":0.0,"publicationDate":"1978-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11930166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ornithine decarboxylase activity in rat liver during phalloidin poisoning. phalloidin中毒大鼠肝脏鸟氨酸脱羧酶活性。

Cytobiologie Pub Date : 1978-12-01

P Antony, H Schimassek

引用次数: 0

Modified desmosomes in cultured epithelial cells. 上皮细胞中修饰的桥粒。

Cytobiologie Pub Date : 1978-12-01

H Zerban, W W Franke

引用次数: 0