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Visualization of mouse DNA transcriptional complexes in mouse kidney cells infected with SV 40 virus. sv40病毒感染小鼠肾细胞中小鼠DNA转录复合物的可视化。
Cytobiologie Pub Date : 1978-12-01
F Puvion-Dutilleul, E May
{"title":"Visualization of mouse DNA transcriptional complexes in mouse kidney cells infected with SV 40 virus.","authors":"F Puvion-Dutilleul,&nbsp;E May","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Electron microscopic studies of transcribed cellular deoxynucleoprotein (DNP) fibers from mouse kidney cells abortively infected with Simian Virus 40 (SV 40) revealed two types of transcriptional complexes. Tandemly repeating units representing ribosomal transcriptional events, although few in number because of the procedure employed, clearly confirmed results of other techniques which revealed the unusually long length of the untranscribed spacer intercepts in mouse nucleolar chromatin. In non-nucleolar arrays the density of the ribonucleoprotein (RNP) fibrils varied, as did the length and configuration of the associated DNP fibers. A statistical correlation between a \"smooth\" appearance of a transcribed portion of a DNP fiber and a high density of nascent RNP fibrils was observed.</p>","PeriodicalId":75770,"journal":{"name":"Cytobiologie","volume":"18 2","pages":"294-308"},"PeriodicalIF":0.0,"publicationDate":"1978-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11431144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Structure of LiCl core particles of 50 S ribosomal subunits from Escherichia coli by electron microscopy. 大肠杆菌50个S核糖体亚基LiCl核粒的电镜结构。
Cytobiologie Pub Date : 1978-12-01
M Boublik, E Spiess, H E Roth, W Hellmann, F Jenkins
{"title":"Structure of LiCl core particles of 50 S ribosomal subunits from Escherichia coli by electron microscopy.","authors":"M Boublik,&nbsp;E Spiess,&nbsp;H E Roth,&nbsp;W Hellmann,&nbsp;F Jenkins","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The structure of 50 S E. coli ribosomal subunits was studied by electron microscopy as these particles were gradually depleted of proteins by incubation with 0.5 to 6.0 m LiCl. Changes observed in the structure of the depleted subunits were correlated with the location of the deleted ribosomal proteins on the control 50 S particle. These changes were particularly striking in the \"crown\" region, the site of a considerable number of the proteins necessary for the biological activity of the 50 S subunit. Protein L 16, the first to be removed by the LiCl treatment, was found to be essential for the structural integrity of the large subunit through interactions with ribosomal proteins residing in the left-hand side crest and the interface. Based on electron microscopic evidence, a scheme was proposed for the structural changes accompanying the stepwise unfolding of the 50 S E. coli subunit by LiCl.</p>","PeriodicalId":75770,"journal":{"name":"Cytobiologie","volume":"18 2","pages":"309-19"},"PeriodicalIF":0.0,"publicationDate":"1978-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11575006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Scanning electron microscopic observations on cells grown in vitro. IV. HeLa cells do not have an upperside-underside polarity. 体外培养细胞的扫描电镜观察。海拉细胞没有上下极性。
Cytobiologie Pub Date : 1978-12-01
N Paweletz, W Liebrich
{"title":"Scanning electron microscopic observations on cells grown in vitro. IV. HeLa cells do not have an upperside-underside polarity.","authors":"N Paweletz,&nbsp;W Liebrich","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Small glass particles are added to HeLa monolayer cell cultures. Landing on top of a flattened cell fingerlike protrusions are surrounding the particles. Later on leading lamellae are formed on the upperside of the cell and the glass becomes more and more surrounded until it appears to be \"incorporated\" completely. The glass particles become attached so firmly that by detaching them the cell surface is destroyed. This speaks against the assumption that cells in monolayers are polarized.</p>","PeriodicalId":75770,"journal":{"name":"Cytobiologie","volume":"18 2","pages":"339-44"},"PeriodicalIF":0.0,"publicationDate":"1978-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11930038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The effect of different tidal volumes of respiration on the transport of H3-palmitic acid through the type II pneumocyte of the rat. 不同潮气量呼吸对h3 -棕榈酸通过大鼠II型肺细胞转运的影响。
Cytobiologie Pub Date : 1978-12-01
B Vidić
{"title":"The effect of different tidal volumes of respiration on the transport of H3-palmitic acid through the type II pneumocyte of the rat.","authors":"B Vidić","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":75770,"journal":{"name":"Cytobiologie","volume":"18 2","pages":"272-80"},"PeriodicalIF":0.0,"publicationDate":"1978-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11783916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Colchicine inhibition of lysosome movement in rat uterine epithelium. 秋水仙碱对大鼠子宫上皮溶酶体运动的抑制作用。
Cytobiologie Pub Date : 1978-12-01
M B Parr, M G Kay, E L Parr
{"title":"Colchicine inhibition of lysosome movement in rat uterine epithelium.","authors":"M B Parr,&nbsp;M G Kay,&nbsp;E L Parr","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":75770,"journal":{"name":"Cytobiologie","volume":"18 2","pages":"374-8"},"PeriodicalIF":0.0,"publicationDate":"1978-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11773509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The influence of Triton X-100 on the nuclear envelope of the isolated liver cell nuclei. Triton X-100对离体肝细胞核核膜的影响。
Cytobiologie Pub Date : 1978-12-01
W M Frederiks, J James, C Arnouts, S Broekhoven, J Morreau
{"title":"The influence of Triton X-100 on the nuclear envelope of the isolated liver cell nuclei.","authors":"W M Frederiks,&nbsp;J James,&nbsp;C Arnouts,&nbsp;S Broekhoven,&nbsp;J Morreau","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In order to obtain more precise information on an eventual presence of extra-membranous lipids in the interior of the nucleus, the effects of Triton X-100 on the lipid content and ultrastructure of isolated rat liver nuclei was investigated. Enzyme markers (a.o. glucose-6-phosphatase) were used to control impurities of the nuclear fractions biochemically along with transmission electron microscopy and qualitative and quantitative light microscopy to check the condition of the nuclei obtained. Treatment of the nuclear fraction with increasing concentrations of Triton X-100 resulted in a decrease of the phospholipid content down to 25% at a Triton X-100/protein ratio of 0.4. A further decrease to 8% was measured at a ratio of 1.5. Electron microscopy of nuclei of the latter group showed nuclei containing outer membrane fragments in 2.5% of their surfaces. The composition of lipids extracted from a nuclear fraction appeared to be markedly changed after treatment with Triton X-100 with an increase of the percentage of neutral lipids and the phospholipids diphosphatidyl-glycerol and spingomyelin. From the chemical and morphological data obtained, the conclusion was drawn that a substantial part of the lipids remaining in the isolated nuclei after treatment with Triton X-100 is localized in both membranes of the nuclear envelope. It cannot however, be excluded that a small portion would be present in the interior of the nuclei.</p>","PeriodicalId":75770,"journal":{"name":"Cytobiologie","volume":"18 2","pages":"254-71"},"PeriodicalIF":0.0,"publicationDate":"1978-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11431143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The fine structure of growing and non-growing whole glia cell preparations. 生长和非生长全胶质细胞的精细结构制备。
Cytobiologie Pub Date : 1978-12-01
V P Collins
{"title":"The fine structure of growing and non-growing whole glia cell preparations.","authors":"V P Collins","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Human glia cells become blocked in G1 if starved of serum. The characteristics of the GI blocked state are flattening on the substrate, and absence of cell translocation, ruffling and macropinocytosis. Re-entry into the cell cycle, as a result of growth factor stimulation, is accompained and even preceded by the return of this cellular locomotion. We have studied the fine structure of intact human glia cells and ultrathin sections of these cells when proliferating normally in vitro, when starved of serum and during their return to the cell cycle following stimulation with mEGF (mouse epidermal growth factor). Particular attention was paid to morphologically definable components of the cellular musculoskeletal system. Proliferating interphase glia generally had a leading lamella containing few organelles and oriented bundles of 7 nm microfilaments with structureless lamellipodia at their tips, which often formed ruffles. The perinuclear area was thick and contained many cell organelles, including mitochondria and secondary lysosomes. Glia starved of serum were thinly spread; their peripheral cytoplasm was filled with a diffuse mat of microfilaments, they had no structureless lamellipodia and their perinuclear areas, although thinner, contained cell organelles in equal amounts and of similar type of those found in proliferating cells. On EGF stimulation, after approximately 2 hours the perinuclear area of the cells thickened, and structureless lamellipodia subsequently appeared at the tips of the leading lamellae, forming ruffles. The cells finally began to translocate, the process being accompained by the reorientation and packing of the microfilaments into bundles. As the kinetics of EGF binding and break down by glia cells are similar to those described for fibroblasts, the findings do not support the concept of EGF receptor interactions inducing ultrastructurally demonstrable microfilament or other musculoskeletal structural changes in the cell. They do, however, define the differing cellular morphologies of motile and immobile structures.</p>","PeriodicalId":75770,"journal":{"name":"Cytobiologie","volume":"18 2","pages":"327-38"},"PeriodicalIF":0.0,"publicationDate":"1978-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11523507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Secretion and organization of the stratified wall of spermatophore in some copepods: ultrastructure and cytochemistry]. [某些桡足动物精包层壁的分泌和组织:超微结构和细胞化学]。
Cytobiologie Pub Date : 1978-12-01
I D Gharagozlou-van Ginneken
{"title":"[Secretion and organization of the stratified wall of spermatophore in some copepods: ultrastructure and cytochemistry].","authors":"I D Gharagozlou-van Ginneken","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The ultrastructure of the wall of the spermatophore of some copepods is studied during its elaboration along the male genital tract. The secretion of this material appears in the form of a loose fibrillar network, sandwiched between a granular layer and a set of thin dense plates. At the level of segment III this layer, which has considerably thickened compared with segment II, shows a more compact aspect. Finally in the spermatophore its organization is similar to that of external cuticles. The typical architecture, the elaboration process as well as the ultrastructural cytochemical characteristics of this wall lead us to discuss the analogies with the peripheral cuticle.</p>","PeriodicalId":75770,"journal":{"name":"Cytobiologie","volume":"18 2","pages":"231-43"},"PeriodicalIF":0.0,"publicationDate":"1978-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11930167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Alterations of cytoskeletal morphologies and growth patterns in human fibroblasts treated with polyethylene glycol. 聚乙二醇处理的人成纤维细胞细胞骨架形态和生长模式的改变。
Cytobiologie Pub Date : 1978-12-01
J W Fuseler, C L Miller, G M Fuller
{"title":"Alterations of cytoskeletal morphologies and growth patterns in human fibroblasts treated with polyethylene glycol.","authors":"J W Fuseler,&nbsp;C L Miller,&nbsp;G M Fuller","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Non-transformed human fibroblasts, strain PA-2, were treated with polyethylene glycol (PEG) in monolayer culture to produce multinucleate fibroblast homokaryons. Antibodies to tubulin or actin were used to monitor cytoplasmic microtubule and actin filament patterns immediately after cytoplasmic fusion, as well as after the fused cells had been in culture for varying amounts of time. The cytoplasmic microtubule complex as increased for a short time after cell fusion and then decreased to resemble the complex seen in control cells. The actin stress fibers were similarly enhanced for a comparable period of time. However, this initial enhancement of the actin stress fibers gradually diminished for approximately one month in culture after which the fibers were greatly reduced in both size and number. Concurrent with the changes in cytoplasmic microtubule and actin fiber complexes, the PEG-treated cells began to show alterations in growth parameters which progressively resembled those characteristic of transformed cell populations. Fusion of normal cells may be an initial step in the transformation of such cells to malignancy.</p>","PeriodicalId":75770,"journal":{"name":"Cytobiologie","volume":"18 2","pages":"345-59"},"PeriodicalIF":0.0,"publicationDate":"1978-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11930039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[An ultrastructural study of acid phosphatase activity localized in bud primordia of an organogenic culture of Nicotiana tabacum L. var. P. 19]. [烟草变种 P. 19 芽原基中酸性磷酸酶活性的超微结构研究]。
Cytobiologie Pub Date : 1978-12-01
M T de Boucaud, C Suire
{"title":"[An ultrastructural study of acid phosphatase activity localized in bud primordia of an organogenic culture of Nicotiana tabacum L. var. P. 19].","authors":"M T de Boucaud, C Suire","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Acid phosphatase activity in particularly organogenic strain of tobacco has been localized in two kinds of tissue: the internal bud primordia and the adjacent tissues. Generally the reactions are weaker in the undifferentiated cells. In differentiating cells, the localization of precipitates from the reaction shows up the continuity of membrane systems such as the endoplasmic reticulum, the nuclear membrane and the vacuoles. The relative weakness of the reactions observed in the vacuoles is in agreement with the rareness of hydrolysis. Parenchymatous cells between the meristems and the surface of the tissue culture undergo autolysis, which seems to help the growth of buds produced by neoformation. Specific and often intense reactions occur in the cell walls.</p>","PeriodicalId":75770,"journal":{"name":"Cytobiologie","volume":"18 2","pages":"281-93"},"PeriodicalIF":0.0,"publicationDate":"1978-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11930169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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