Clinical and experimental pharmacology & physiology. Supplement最新文献_第7页

Cardiovascular and renal protective effects of losartan in spontaneously hypertensive rats with diabetes mellitus. 氯沙坦对自发性高血压合并糖尿病大鼠的心血管和肾脏保护作用。

Clinical and experimental pharmacology & physiology. Supplement Pub Date : 1995-12-01 DOI: 10.1111/j.1440-1681.1995.tb02956.x

M Kohzuki, M Yasujima, P F Liu, K Obara, M Kanazawa, K Yoshida, T Saito, T Sato, K Abe

引用次数: 12

Preparation of a monoclonal antibody against mouse brain natriuretic peptide (BNP) and tissue distribution of BNP in mice. 抗小鼠脑钠肽(BNP)单克隆抗体的制备及BNP在小鼠组织中的分布。

Clinical and experimental pharmacology & physiology. Supplement Pub Date : 1995-12-01 DOI: 10.1111/j.1440-1681.1995.tb02874.x

M Nakagawa, I Tanaka, S Suga, Y Ogawa, N Tamura, M Goto, A Sugawara, T Yoshimasa, H Itoh, M Mukoyama

{"title":"Preparation of a monoclonal antibody against mouse brain natriuretic peptide (BNP) and tissue distribution of BNP in mice.","authors":"M Nakagawa, I Tanaka, S Suga, Y Ogawa, N Tamura, M Goto, A Sugawara, T Yoshimasa, H Itoh, M Mukoyama","doi":"10.1111/j.1440-1681.1995.tb02874.x","DOIUrl":"https://doi.org/10.1111/j.1440-1681.1995.tb02874.x","url":null,"abstract":"1. In order to explore the significance of brain natriuretic peptide (BNP), a cardiac hormone secreted from the ventricle, in mice, we prepared a monoclonal antibody against mouse BNP (mBNP) and established a specific radioimmunoassay (RIA) for mBNP. 2. A monoclonal antibody, KY-mBNP-I, was prepared by the fusion of mouse myeloma cells X63-Ag8.653 with spleen cells of the BALB/c mouse immunized with synthetic mBNP[108-121] conjugated to bovine thyroglobulin. KY-mBNP-I belonged to an IgG2a subclass and showed a high affinity for mBNP (Ka = 1.8 x 10(11) mol/L-1). 3. The RIA established that using KY-mBNP-I was highly sensitive and specific for mBNP, with an IC50 value of 3 fmol/tube and cross-reactivities of less than 0.003% with related natriuretic peptides. mBNP-like immunoreactivity (mBNP-LI) was detected in the mouse atrium (0.35 +/- 0.02 nmol/g), ventricle (20.5 +/- 0.5 pmol/g) and kidney (0.50 +/- 0.05 pmol/g), but not in other tissues including brain. 4. Gel filtration analysis revealed that the major component of tissue mBNP-LI was co-eluted with synthetic mBNP[77-121], a 45-amino acid mature peptide. 5. The monoclonal antibody and RIA for mBNP established here will provide useful tools to investigate the functional significance of BNP in mice, coupled with the genetic engineering approach.","PeriodicalId":75710,"journal":{"name":"Clinical and experimental pharmacology & physiology. Supplement","volume":"22 1","pages":"S186-7"},"PeriodicalIF":0.0,"publicationDate":"1995-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1440-1681.1995.tb02874.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20027238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Effect of felodipine treatment and withdrawal on blood pressure and cardiovascular structure in New Zealand genetically hypertensive rats. 非洛地平治疗和停药对新西兰遗传性高血压大鼠血压和心血管结构的影响。

Clinical and experimental pharmacology & physiology. Supplement Pub Date : 1995-12-01 DOI: 10.1111/j.1440-1681.1995.tb02939.x

J M Ledingham, E L Phelan, M A Cross, R Laverty

{"title":"Effect of felodipine treatment and withdrawal on blood pressure and cardiovascular structure in New Zealand genetically hypertensive rats.","authors":"J M Ledingham, E L Phelan, M A Cross, R Laverty","doi":"10.1111/j.1440-1681.1995.tb02939.x","DOIUrl":"https://doi.org/10.1111/j.1440-1681.1995.tb02939.x","url":null,"abstract":"1. Four groups of New Zealand genetically hypertensive (GH) rats were treated with felodipine as follows: (A) from ages 4-12 weeks; (B) 4-20 weeks; (C) 4-12 weeks then withdrawn from felodipine till age 20 weeks; and (D) 12-20 weeks. 2. Effects on blood pressure (BP), left ventricular (LV) weight and the structure of myograph-mounted mesenteric resistance arteries (MRA) were measured. 3. BP was about 170 mmHg in 4 week old GH rats and did not change substantially from this value in groups A and B but rose to over 230 mmHg in untreated controls. In rats of group D, started on felodipine at 12 weeks, BP fell rapidly and was 173 mmHg at 20 weeks. On withdrawal of felodipine at 12 weeks (group C) BP rose rapidly and exceeded control levels at 20 weeks. 4. LV mass was significantly lower (P < 0.001) in all groups (A, B and D) killed while on felodipine but rose to control levels in the rats taken off felodipine. 5. In the four treated groups there were no significant changes in MRA structure (lumen diameter, medial thickness, media/lumen ratio and volume of medial tissue) as measured on the myograph. 6. Felodipine induces substantial falls in BP in GH rats which are sufficient to prevent cardiac hypertrophy but do not alter MRA structure. Resistance artery structure in GH rats does not seem to relate to the level of hypertension, and may therefore not have a necessary role in the pathogenesis of this hypertensive strain.","PeriodicalId":75710,"journal":{"name":"Clinical and experimental pharmacology & physiology. Supplement","volume":"22 1","pages":"S326-8"},"PeriodicalIF":0.0,"publicationDate":"1995-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1440-1681.1995.tb02939.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20027476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Decreased sensitivity to beta-adrenergic stimulation of the ventricular cells isolated from the spontaneously hypertensive rat heart. 自发性高血压大鼠心脏分离心室细胞对β -肾上腺素能刺激的敏感性降低。

Clinical and experimental pharmacology & physiology. Supplement Pub Date : 1995-12-01 DOI: 10.1111/j.1440-1681.1995.tb02840.x

Y Habuchi, L L Lu, S Okamoto, T Komori, H Takahashi, J Morikawa, M Yoshimura

引用次数: 7

Synergistic effects of Bay K 8644 and bradykinin on norepinephrine release in the hypothalamus of spontaneously hypertensive rats. Bay k8644和缓激肽对自发性高血压大鼠下丘脑去甲肾上腺素释放的协同作用。

Clinical and experimental pharmacology & physiology. Supplement Pub Date : 1995-12-01 DOI: 10.1111/j.1440-1681.1995.tb02968.x

K Tsuda, S Tsuda, I Nishio, Y Masuyama, M Goldstein

{"title":"Synergistic effects of Bay K 8644 and bradykinin on norepinephrine release in the hypothalamus of spontaneously hypertensive rats.","authors":"K Tsuda, S Tsuda, I Nishio, Y Masuyama, M Goldstein","doi":"10.1111/j.1440-1681.1995.tb02968.x","DOIUrl":"https://doi.org/10.1111/j.1440-1681.1995.tb02968.x","url":null,"abstract":"1. In the present study, we examined the effects of Bay K 8644, a dihydropyridine (DHP)-sensitive Ca2+ channel agonist, and bradykinin on norepinephrine release in the hypothalamus of spontaneously hypertensive rats (SHR). 2. In the preliminary studies using Sprague-Dawley rats, Bay K 8644 by itself had no significant effects on the stimulation-evoked [3H]-norepinephrine release from hypothalamic slices. Bradykinin increased the stimulation-evoked [3H]-norepinephrine release in a dose-related fashion. The facilitatory effects of bradykinin on norepinephrine release were potentiated by Bay K 8644. 3. In SHR, Bay K 8644 significantly increased the stimulation-evoked norepinephrine release from hypothalamic slices. However, exposure of slices to Bay K 8644 caused no significant effects on norepinephrine release in Wistar-Kyoto (WKY) rats. The effects of Bay K 8644 in combination with bradykinin on the stimulation-evoked norepinephrine release were also greater in SHR than in WKY rats. 4. These results demonstrate that Bay K 8644 significantly potentiated the facilitatory effects of bradykinin on norepinephrine release in rat hypothalamus. The finding indicates a possible interaction of bradykinin with DHP-sensitive Ca2+ channels in the central nervous system. Furthermore, the pronounced effects of Bay K 8644 and bradykinin in SHR suggest that bradykinin-related Ca2+ channels might have a role in the regulation of norepinephrine release in the hypothalamus of SHR.","PeriodicalId":75710,"journal":{"name":"Clinical and experimental pharmacology & physiology. Supplement","volume":"22 1","pages":"S54-7"},"PeriodicalIF":0.0,"publicationDate":"1995-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1440-1681.1995.tb02968.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20027649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Alteration of baroreceptor and chemoreceptor reflexes in spontaneously hypertensive rats. 自发性高血压大鼠压力感受器和化学感受器反射的改变。

Clinical and experimental pharmacology & physiology. Supplement Pub Date : 1995-12-01 DOI: 10.1111/j.1440-1681.1995.tb02970.x

H Ohta, W T Talman

{"title":"Alteration of baroreceptor and chemoreceptor reflexes in spontaneously hypertensive rats.","authors":"H Ohta, W T Talman","doi":"10.1111/j.1440-1681.1995.tb02970.x","DOIUrl":"https://doi.org/10.1111/j.1440-1681.1995.tb02970.x","url":null,"abstract":"1. Electrical stimulation of the aortic depressor nerve elicited depressor and bradycardiac responses in both spontaneously hypertensive rats (SHR) and Wistar Kyoto (WKY) rats, but in spontaneously breathing animals responses were weaker in SHR than in WKY. 2. Responses did not significantly differ in artificially ventilated animals except that high stimulation intensity elicited significantly greater bradycardiac responses in WKY. A decrease in ventilatory rate occurred in both strains. 3. Electrical stimulation of the carotid sinus nerve (CSN) elicited an initial pressor response followed by a depressor response with a minimal change in heart rate in either strain. The pressor response in SHR was greater than in WKY. Depressor responses in SHR were weaker than those in WKY with or without artificial ventilation. CSN stimulation increased ventilatory rate in both strains but the frequency-related responses differed at higher stimulation intensity. 4. These results suggest that there are alterations in the function of central or afferent components of chemoreceptor and baroreceptor reflexes in SHR in addition to previously described structural changes at the level of peripheral sensory receptors.","PeriodicalId":75710,"journal":{"name":"Clinical and experimental pharmacology & physiology. Supplement","volume":"22 1","pages":"S60-1"},"PeriodicalIF":0.0,"publicationDate":"1995-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1440-1681.1995.tb02970.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20027651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 21

Studies on pregnancy hypertension and IUGR-SFD: effects of drugs on the blood vessels in the placenta of pregnant SHRSP. 妊娠高血压与IUGR-SFD的研究:药物对妊娠SHRSP胎盘血管的影响。

Clinical and experimental pharmacology & physiology. Supplement Pub Date : 1995-12-01 DOI: 10.1111/j.1440-1681.1995.tb02920.x

I Fuchi, K Noda, Y Matsubara

引用次数: 5

Enhanced contractile responses mediated by different 5-HT receptor subtypes in basilar arteries, superior mesenteric arteries and thoracic aortas from stroke-prone spontaneously hypertensive rats. 不同5-HT受体亚型介导的卒中易发自发性高血压大鼠基底动脉、肠系膜上动脉和胸主动脉收缩反应增强

Clinical and experimental pharmacology & physiology. Supplement Pub Date : 1995-12-01 DOI: 10.1111/j.1440-1681.1995.tb02986.x

Y Nishimura, A Suzuki

{"title":"Enhanced contractile responses mediated by different 5-HT receptor subtypes in basilar arteries, superior mesenteric arteries and thoracic aortas from stroke-prone spontaneously hypertensive rats.","authors":"Y Nishimura, A Suzuki","doi":"10.1111/j.1440-1681.1995.tb02986.x","DOIUrl":"https://doi.org/10.1111/j.1440-1681.1995.tb02986.x","url":null,"abstract":"1. The contractile effects of 5-hydroxytryptamine (5-HT) in isolated ring preparations of basilar arteries (BA) thoracic aortas (TA) and superior mesenteric arteries (SMA) from stroke-prone spontaneously hypertensive rats (SHRSP) and Wistar-Kyoto (WKY) rats were investigated pharmacologically. 2. The pD2 values (expressed as a negative logarithm of of EC50) for 5-HT in BA of SHRSP were greater than those of WKY. Increased pD2 values for 5-HT were also found in SMA and TA of SHRSP when compared to WKY. 3. Ketanserin (a 5-HT2 antagonist) produced a biphasic displacement of the concentration-response curves for 5-HT in BA of WKY and SHRSP but elicited a parallel rightward shift of the 5-HT curve in SMA and TA of the two groups. 4. 5-CT (a 5-HT1 agonist)-induced contractions and their pD2 values in the presence of ketanserin were larger in BA of SHRSP than in those of WKY, while 5-CT did not contract SMA or TA in either group. 5. No significant difference was found in the contractile response induced by alpha-methyl-5-HT (a 5-HT2 agonist) in BA from SHRSP and WKY, while the pD2 values for alpha-methyl-5-HT were increased in SMA and TA from SHRSP when compared to WKY. 6. These results suggest that the hyperresponsiveness to 5-HT found in SHRSP arteries may be mediated by different 5-HT receptor subtypes, that is, by 5-HT1 in BA and by 5-HT2 in SMA and TA.","PeriodicalId":75710,"journal":{"name":"Clinical and experimental pharmacology & physiology. Supplement","volume":"22 1","pages":"S99-101"},"PeriodicalIF":0.0,"publicationDate":"1995-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1440-1681.1995.tb02986.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20027880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Regulation of vascular contraction by ionic matrix surfaces of the smooth muscle cell: changes in SHR? 平滑肌细胞离子基质表面对血管收缩的调控:SHR的变化?

Clinical and experimental pharmacology & physiology. Supplement Pub Date : 1995-12-01 DOI: 10.1111/j.1440-1681.1995.tb02898.x

G M Kravtsov, C Y Kwan

{"title":"Regulation of vascular contraction by ionic matrix surfaces of the smooth muscle cell: changes in SHR?","authors":"G M Kravtsov, C Y Kwan","doi":"10.1111/j.1440-1681.1995.tb02898.x","DOIUrl":"https://doi.org/10.1111/j.1440-1681.1995.tb02898.x","url":null,"abstract":"1. KCl-induced contractions in aortic rings of spontaneously hypertensive rats (SHR) and two normotensive strains, Sprague-Dawley (SD) and Wistar-Kyoto (WKY) rats, were studied. 2. The contraction elicited by isotonic Na-free, KCl solution containing 2.5 mmol/L Ca and 1 mmol/L Mg was concentration-dependently inhibited by Na and K. Maximal inhibition was induced by 20 mmol/L NaCl in WKY and SHR (65 and 85%, respectively) and by 60 mmol/L NaCl in SD (50%). KCl (10-40 mmol/L) had no effect on SD but produced almost full relaxation in WKY and about 50% relaxation in SHR. 3. Isotonic Ca-free, Na-free high Mg (2 mmol/L), K-solution caused strong phasic contractions in WKY and SHR, but weak responses in SD. 4. Aortic rings of SD, WKY and SHR developed strong, sustained and reproducible contractions in isotonic Ca-free, Na-free, K-solution containing EDTA, which were fully relaxed by 1.2, 1.6 and 8 mmol/L Na, respectively. 5. We suggest that the primary mechanism of KCl-contraction is potential-independent competition between monovalent and divalent cations for binding sites on both surfaces of the plasma membrane (e.g. K with Mg and Na with Ca) and that differences in aortic contractility in SHR, WKY and SD merely reflect strain differences in the composition of the ionic matrix, independent of hypertension.","PeriodicalId":75710,"journal":{"name":"Clinical and experimental pharmacology & physiology. Supplement","volume":"22 1","pages":"S237-9"},"PeriodicalIF":0.0,"publicationDate":"1995-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1440-1681.1995.tb02898.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20028031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Characterization of retinal vascular abnormalities in lean and obese spontaneously hypertensive rats. 瘦和肥胖自发性高血压大鼠视网膜血管异常的特征。

Clinical and experimental pharmacology & physiology. Supplement Pub Date : 1995-12-01 DOI: 10.1111/j.1440-1681.1995.tb02850.x

S S Huang, S A Khosrof, R J Koletsky, B A Benetz, P Ernsberger

引用次数: 16