Clinical and experimental pharmacology & physiology. Supplement最新文献_第10页

Augmented expression of the endothelin-A receptor gene in cultured mesangial cells from stroke-prone spontaneously hypertensive rats. 内皮素a受体基因在卒中易发自发性高血压大鼠系膜细胞中的表达增强。

Clinical and experimental pharmacology & physiology. Supplement Pub Date : 1995-12-01 DOI: 10.1111/j.1440-1681.1995.tb02876.x

J Hiraoka, H Arai, T Yoshimasa, K Takaya, Y Miyamoto, J Yamashita, S Suga, Y Ogawa, G Shirakami, H Itoh

引用次数: 5

Long-term therapeutic effects of ace inhibitor and calcium antagonists on hypertensive vascular lesions in M-SHRSP. 血管紧张素转换酶抑制剂和钙拮抗剂对M-SHRSP高血压血管病变的长期治疗效果。

Clinical and experimental pharmacology & physiology. Supplement Pub Date : 1995-12-01 DOI: 10.1111/j.1440-1681.1995.tb02937.x

Y Ohta, T Chikugo, T Suzuki

引用次数: 5

Molecular mechanisms of vascular hypertrophy in the spontaneously hypertensive rat. 自发性高血压大鼠血管肥大的分子机制。

Clinical and experimental pharmacology & physiology. Supplement Pub Date : 1995-12-01 DOI: 10.1111/j.1440-1681.1995.tb02844.x

P Marche, T Herembert, D L Zhu

{"title":"Molecular mechanisms of vascular hypertrophy in the spontaneously hypertensive rat.","authors":"P Marche, T Herembert, D L Zhu","doi":"10.1111/j.1440-1681.1995.tb02844.x","DOIUrl":"https://doi.org/10.1111/j.1440-1681.1995.tb02844.x","url":null,"abstract":"1. In primary hypertension, an abnormally high vascular resistance can be explained in terms of alterations in vessel wall structure. Arterial cell hypertrophy and/or hyperplasia have been implicated as playing a central role in the vascular abnormalities noted in spontaneously hypertensive rats (SHR). Cultured arterial cells appear therefore as attractive models for studying in vitro the mechanisms whereby cells originating from hypertensives exhibit hyperproliferation and/or hyperresponsiveness. 2. This review summarized our present knowledge on growth and related biochemical events of cultured SHR-derived vascular smooth muscle cells or aortic adventitial fibroblasts, in response to various polypeptide growth factors and vasoactive agents. 3. Exaggerated growth response to various mitogens in cultured SHR-derived vascular cells has been well documented. However, the molecular mechanisms of abnormal growth in SHR remain unknown. This abnormality seems not to be a consequence of the alterations at the levels of receptors or of some key mitogenic events of early signalling pathways such as phospholipase C, protein kinase C and G-proteins. Further studies should therefore focus on the more distal events related to cell growth.","PeriodicalId":75710,"journal":{"name":"Clinical and experimental pharmacology & physiology. Supplement","volume":"22 1","pages":"S114-6"},"PeriodicalIF":0.0,"publicationDate":"1995-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1440-1681.1995.tb02844.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20027638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

Increased immunoreactivities for the basic fibroblast growth factor and its receptor in astrocytes at the site of cerebral lesions and oedematous change in SHR. SHR脑损伤部位星形胶质细胞中碱性成纤维细胞生长因子及其受体的免疫反应性增强和水肿改变。

Clinical and experimental pharmacology & physiology. Supplement Pub Date : 1995-12-01 DOI: 10.1111/j.1440-1681.1995.tb02909.x

H Kataoka, E Yamada, Y Hayase, F Hazama

{"title":"Increased immunoreactivities for the basic fibroblast growth factor and its receptor in astrocytes at the site of cerebral lesions and oedematous change in SHR.","authors":"H Kataoka, E Yamada, Y Hayase, F Hazama","doi":"10.1111/j.1440-1681.1995.tb02909.x","DOIUrl":"https://doi.org/10.1111/j.1440-1681.1995.tb02909.x","url":null,"abstract":"1. To obtain information about changes of basic fibroblast growth factor (bFGF) in the brain in chronic hypertension, we immunohistochemically studied the distribution and level of bFGF and its receptor in the brain of stroke-prone spontaneously hypertensive rats (SHRSP). 2. In the control normotensive rats, immunoreactivity for bFGF was demonstrated in nerve cells, while there was almost no reactivity in astrocytes. 3. In SHRSP, there was a marked immunoreactivity in the densely accumulated reactive cells, particularly astrocytes, in and around cerebral cortical lesions. Slightly increased reaction for bFGF was found in the nerve cells around lesions. Astrocytes in the subcortical white matter on both ipsi- and contralateral sides of the cortical lesion also showed immunoreactivity for bFGF. The location of increased bFGF expression in SHRSP corresponded very well with the site of extravasated plasma fluid demonstrated by anti-fibrinogen antibody. Electron microscopically, bFGF was shown in astrocytes along the rough endoplasmic reticulum suggesting the growth factor to be produced in the cells and not to be taken up from the surroundings. Expression of FGF-receptor was also demonstrated in reactive astrocytes in the oedematous cortical portion around lesion and in the oedematous subcortical white matter. 4. These findings indicate the possibility that oedema and the simultaneously generated free radicals or some extravasated plasma components express bFGF in astrocytes and probably in nerve cells as well as FGF-receptor in astrocytes, and that the thus expressed bFGF and its receptor play some role in the sequence of developmental events of hypertensive cerebral lesions.","PeriodicalId":75710,"journal":{"name":"Clinical and experimental pharmacology & physiology. Supplement","volume":"22 1","pages":"S262-4"},"PeriodicalIF":0.0,"publicationDate":"1995-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1440-1681.1995.tb02909.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20027646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Biochemical characterization of the fibres of collagen and elastin in the aortic wall of SHRSP. SHRSP主动脉壁胶原蛋白和弹性蛋白纤维的生化特征。

Clinical and experimental pharmacology & physiology. Supplement Pub Date : 1995-12-01 DOI: 10.1111/j.1440-1681.1995.tb02853.x

S Matsumura, S Kawazoye, S F Tian, T Takashima, T Sunaga, S Toda, N Fujitani, H Higashino, A Suzuki

{"title":"Biochemical characterization of the fibres of collagen and elastin in the aortic wall of SHRSP.","authors":"S Matsumura, S Kawazoye, S F Tian, T Takashima, T Sunaga, S Toda, N Fujitani, H Higashino, A Suzuki","doi":"10.1111/j.1440-1681.1995.tb02853.x","DOIUrl":"https://doi.org/10.1111/j.1440-1681.1995.tb02853.x","url":null,"abstract":"1. Vessel wall fragments consisting of collagen, elastin and other insoluble proteins were prepared from the aortas of 6 month old WKY and SHRSP and dead, elderly humans. 2. Prolonged incubations of these fragments with pepsin below or at 30 degrees C resulted in different amounts of insoluble materials containing similar or larger proportions of collagen and other insoluble proteins than the respective vessel fragments. The amounts of the pepsin-insoluble materials obtained from SHRSP were larger than those from WKY but were much smaller than those from elderly humans. 3. The elastins isolated from the vessel fragments were solubilized by pepsin much more effectively than the respective vessel fragments. 4. The pepsin-insoluble materials from WKY were composed of thin mesh-shaped materials, while these materials from both rats did not contain a significant number of distinctive fibrils of collagen, the materials from elderly humans did contain numerous distinctive fibrils of collagen. 5. Large fractions of both the collagen and other proteins in the pepsin-insoluble materials were solubilized by incubation with a crude bacterial collagenase below 30 degrees C or by incubation with pepsin above 40 degrees C where the triple-helical regions of the collagens were unfolded. 6. These results appear to indicate that the aortic wall of SHRSP contains larger amounts of some insoluble components that immobilize the collagen fibrils than that of WKY, but the aortic walls of elderly humans contain much larger amounts of these components than that of SHRSP.","PeriodicalId":75710,"journal":{"name":"Clinical and experimental pharmacology & physiology. Supplement","volume":"22 1","pages":"S136-8"},"PeriodicalIF":0.0,"publicationDate":"1995-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1440-1681.1995.tb02853.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20027860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Temporal and spatial properties of slow waves in the electroencephalogram of spontaneously hypertensive rats. 自发性高血压大鼠脑电图慢波的时空特征。

Clinical and experimental pharmacology & physiology. Supplement Pub Date : 1995-12-01 DOI: 10.1111/j.1440-1681.1995.tb02921.x

S Chichibu, Y Ohta, T Chikugo, T Suzuki

引用次数: 4

Hypotensive effects of a new transdermal delivery system for clonidine (M-5041T) in spontaneously and renal hypertensive rats. 一种新的可乐定(M-5041T)透皮给药系统对自发性和肾性高血压大鼠的降压作用。

Clinical and experimental pharmacology & physiology. Supplement Pub Date : 1995-12-01 DOI: 10.1111/j.1440-1681.1995.tb02960.x

R Ishii, T Naruse, T Tagawa, T Ishida, K Namba

{"title":"Hypotensive effects of a new transdermal delivery system for clonidine (M-5041T) in spontaneously and renal hypertensive rats.","authors":"R Ishii, T Naruse, T Tagawa, T Ishida, K Namba","doi":"10.1111/j.1440-1681.1995.tb02960.x","DOIUrl":"https://doi.org/10.1111/j.1440-1681.1995.tb02960.x","url":null,"abstract":"1. Antihypertensive effects of a new transdermal delivery system for clonidine (clonidine tape; M-50417) were investigated in spontaneously hypertensive rats (SHR), 2-kidney, 1-clip renal hypertensive rats (RHR) and deoxycorticosterone acetate/salt (DOCA/Salt) hypertensive rats. 2. M-5041T (0.5-4.5 mg/kg) elicited long-lasting hypotensive effects and bradycardia in a dose-dependent manner during 24 h patching in three hypertensive models compared with oral clonidine (100 mu g/kg). 3. The most hypotensive effect of M-5041T was observed in DOCA/salt hypertensive rats. 4. Co-administration of M-5041T with either trichloromethiazide (1 mg/kg, orally) or nifedipine (3 mg/kg, orally) at each dose without hypotensive effects per se induced significant hypotension in SHR. 5. Repeated administrations of M-5041T (1.5 mg/kg per day) for a consecutive 7 days produced significant hypotensive effects at postpatching 6 h, and recovered a postpatching 24 h in SHR. 6. Repetitive M-5041T administrations displayed no tolerance on the hypotensive effects and were devoid of any withdrawal syndrome. 7. These findings suggest that M-5041T may serve as an efficiently useful antihypertensive transdermal delivery system in humans.","PeriodicalId":75710,"journal":{"name":"Clinical and experimental pharmacology & physiology. Supplement","volume":"22 1","pages":"S374-6"},"PeriodicalIF":0.0,"publicationDate":"1995-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1440-1681.1995.tb02960.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20028037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mitochondrial abnormalities in hypertrophied myocardium of stroke-prone spontaneously hypertensive rats. 卒中易发自发性高血压大鼠肥大心肌线粒体异常。

Clinical and experimental pharmacology & physiology. Supplement Pub Date : 1995-12-01 DOI: 10.1111/j.1440-1681.1995.tb02911.x

T Tokoro, H Ito, O Maenishi, T Suzuki

{"title":"Mitochondrial abnormalities in hypertrophied myocardium of stroke-prone spontaneously hypertensive rats.","authors":"T Tokoro, H Ito, O Maenishi, T Suzuki","doi":"10.1111/j.1440-1681.1995.tb02911.x","DOIUrl":"https://doi.org/10.1111/j.1440-1681.1995.tb02911.x","url":null,"abstract":"1. To clarify the pathogenesis of cardiac disorders in SHRSP which showed severe cardiac hypertrophy and myocardial degeneration in the hypertensive stage, restriction fragment length polymorphisms (RFLP) of mitochondrial DNA (mtDNA), and morphological and functional changes of mitochondria were examined. 2. Morphologically, the mitochondrial size showed a wider range of distribution in SHRSP both at prehypertensive and hypertensive stage compared to those in age-matched WKY. 3. Isocitrate dehydrogenase (ICDH) activity, but not superoxide dismutase (SOD) activity, was higher in the young SHRSP, whereas both enzyme activities were lower in the mature SHRSP than in the age-matched WKY. 4. RFLP analysis by electrophoresis revealed that the loss of two restriction sites for Rsa I in the myocardial mtDNA from the SHRSP, but not in that from the WKY. 5. These findings suggest that the structural changes of mtDNA could be related, at least partly, to morphological and functional changes of mitochondria in the SHRSP myocardium.","PeriodicalId":75710,"journal":{"name":"Clinical and experimental pharmacology & physiology. Supplement","volume":"22 1","pages":"S268-9"},"PeriodicalIF":0.0,"publicationDate":"1995-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1440-1681.1995.tb02911.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20026283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Gene expression of prostacyclin receptor in the hypertrophied heart of spontaneously hypertensive rats. 前列环素受体在自发性高血压大鼠肥厚心脏中的基因表达。

Clinical and experimental pharmacology & physiology. Supplement Pub Date : 1995-12-01 DOI: 10.1111/j.1440-1681.1995.tb02912.x

O Nakagawa, Y Sasaki, I Tanaka, T Usui, T Sando, S Muro, K Mori, H Itoh, T Yoshimasa, S Narumiya

{"title":"Gene expression of prostacyclin receptor in the hypertrophied heart of spontaneously hypertensive rats.","authors":"O Nakagawa, Y Sasaki, I Tanaka, T Usui, T Sando, S Muro, K Mori, H Itoh, T Yoshimasa, S Narumiya","doi":"10.1111/j.1440-1681.1995.tb02912.x","DOIUrl":"https://doi.org/10.1111/j.1440-1681.1995.tb02912.x","url":null,"abstract":"1. Prostacyclin elicits potent vasodilation and inhibition of platelet aggregation through binding to its membrane receptor. The impairment of prostacyclin receptor activity is implicated in various human cardiovascular diseases. We recently succeeded in molecular cloning of cDNA for the mouse, rat, and human prostacyclin receptors. 2. In the present study, we examined the mRNA expression of the prostacyclin receptor in various rat tissues, and further investigated its gene expression in the hypertrophied cardiac ventricles of stroke-prone spontaneously hypertensive rats (SHRSP). 3. In rat tissues, a single RNA band of approximately 3.7 kb was detected by northern blotting analysis using rat prostacyclin receptor cDNA as a probe. In adult Wistar rats, abundant mRNA expression was observed in the aorta, lung and spleen. Substantial amounts of transcript were expressed in the heart, pancreas, thymus and stomach. In contrast, no mRNA expression was detected in the brain. 4. We further examined the mRNA expression of the prostacyclin receptor in the ventricles of 21 week old SHRSP. The ventricles of SHRSP showed remarkable hypertrophy, compared with those of age-matched Wistar-Kyoto (WKY) rats. The expression of prostacyclin receptor mRNA in the hypertrophied ventricles of SHRSP was almost equivalent to that in the ventricles of WKY. 5. The present study revealed the gene expression of the prostacyclin receptor in various rat tissues, and further demonstrated the receptor mRNA expression in hypertensive cardiac hypertrophy. The present study will give a clue to investigate the clinical implication of prostacyclin and its receptor.","PeriodicalId":75710,"journal":{"name":"Clinical and experimental pharmacology & physiology. Supplement","volume":"22 1","pages":"S270-2"},"PeriodicalIF":0.0,"publicationDate":"1995-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1440-1681.1995.tb02912.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20026284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Regional distribution of superoxide dismutase in the brain and myocardium of the stroke-prone spontaneously hypertensive rat. 超氧化物歧化酶在卒中易发自发性高血压大鼠脑及心肌的区域分布。

Clinical and experimental pharmacology & physiology. Supplement Pub Date : 1995-12-01 DOI: 10.1111/j.1440-1681.1995.tb02863.x

S Kimoto, S Nishida, K Funasaka, T Nakano, K Teramoto, T T Tomura

{"title":"Regional distribution of superoxide dismutase in the brain and myocardium of the stroke-prone spontaneously hypertensive rat.","authors":"S Kimoto, S Nishida, K Funasaka, T Nakano, K Teramoto, T T Tomura","doi":"10.1111/j.1440-1681.1995.tb02863.x","DOIUrl":"https://doi.org/10.1111/j.1440-1681.1995.tb02863.x","url":null,"abstract":"1. Enzyme activities and contents of manganese and copper-zinc superoxide dismutase (Mn-, Cu/Zn-SOD) and oxygen free-radical scavengers were determined in the myocardium (right, left ventricle) and brain (cerebral cortex, hippocampus) of 15 and 31 week old stroke-prone spontaneously hypertensive rats (SHRSP). 2. In 15 week old SHRSP myocardium, both Mn- and Cu/Zn-SOD activities were higher but in 31 week old SHRSP, these were lower than that in Wistar-Kyoto (WKY) rats. Further, correlation between Mn-SOD content and activity in 31 week old SHRSP myocardium showed that specific activity was lower than that in WKY. 3. In 15 and 31 week old SHRSP cerebral cortex and hippocampus, SOD content and activity showed a tendency to be lower than that in WKY. 4. These results indicate that enzymatically inactive or low-active Mn-SOD protein exists in SHRSP myocardium, and that the alteration of SOD may be one of the causative factors for the vulnerability of the myocardium and brain against O2-radicals.","PeriodicalId":75710,"journal":{"name":"Clinical and experimental pharmacology & physiology. Supplement","volume":"22 1","pages":"S160-1"},"PeriodicalIF":0.0,"publicationDate":"1995-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1440-1681.1995.tb02863.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20026704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11