R Cedillo-Rivera, J A Enciso-Moreno, A Martínez-Palomo, G Ortega Pierres
{"title":"Isolation and axenization of Giardia lamblia isolates from symptomatic and asymptomatic patients in Mexico.","authors":"R Cedillo-Rivera, J A Enciso-Moreno, A Martínez-Palomo, G Ortega Pierres","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Infection of the small intestine of humans with the parasitic protozoan Giardia lamblia may have an asymptomatic course, or else, may produce acute or chronic diarrhea. In order to establish if the different clinical outcome of giardiasis in children could be due, at least partially, to strain differences, isolates from asymptomatic and symptomatic cases studied in Mexico City during 1986 and 1987 were cultured under axenic conditions. With modifications of available methods for the isolation of G. lamblia from cysts in stools, we obtained 19 axenic isolates: 5 from symptomatic patients and 14 from asymptomatic cyst carriers. The isolation procedure involved: (1) concentration and cleaning of cysts through centrifugation in sucrose gradients; (2) excystment induction in acid solution; (3) culture in modified TYI-S-33 medium, and (4) axenization of isolates using ceftriaxone and Amphotericin B. Results indicate that isolates from carriers and from symptomatic cases of giardiasis are equally amenable to isolation and axenization. The Giardia isolates obtained are being studied to analyze differences in isoenzyme pattern, antigenicity, and molecular markers.</p>","PeriodicalId":75554,"journal":{"name":"Archivos de investigacion medica","volume":"22 1","pages":"79-85"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12983026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R Barroso-Moguel, J Villeda-Hernández, M Méndez-Armenta
{"title":"Medical causes and effects of cocaine abuse.","authors":"R Barroso-Moguel, J Villeda-Hernández, M Méndez-Armenta","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Historical data concerning the use of cocaine, its epidemiology, chemistry and pharmacology, as well as its medical complications and treatment, in both acute intoxication and chronic addiction is reported. Its repercussion and damage upon the nervous system with neurologic and psychiatric alterations is also reported. The frequency of cerebral hemorrhages and myocardial, and other visceral infarctions due to vascular lesions are discussed. Various pulmonary lesions produced by the different routes used and the multiple obstetric problems during pregnancy, such as abnormal labour with products showing cerebral damage and teratogenic lesions due to the use of cocaine are presented. Finally, the present prophylactic campaigns against the use of cocaine and other drugs are mentioned.</p>","PeriodicalId":75554,"journal":{"name":"Archivos de investigacion medica","volume":"22 1","pages":"3-8"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12981654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R S Goldsmith, I G Kagan, R Zárate, M A Reyes-González, J Cedeno-Ferreira
{"title":"Low Toxoplasma antibody prevalence in serologic surveys of humans in southern Mexico.","authors":"R S Goldsmith, I G Kagan, R Zárate, M A Reyes-González, J Cedeno-Ferreira","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Antibody prevalence to Toxoplasma gondii among residents of a Pacific coastal region of Oaxaca State, Mexico is among low rates reported worldwide. From 60 small, rural communities, 3229 persons from ages 6 months to over 70 years provided blood specimens that were stored on filter papers. 124 (3.8%) of the eluates were seropositive (positive titer greater than 1:256) in the indirect hemagglutination test, and 43 (1.3%) had titers greater than 1:1024. Seropositive rates increased by age group and females were positive twice as often as males. There was no difference in rates for persons who lived at sea level, compared to those who lived between 600 and 1800 meters. In two small communities in which the number of persons tested were a large percentage of the total population, seropositive rates were 1.0 and 1.9%, respectively. The probable explanation for these low rates is the near absence of cats and paucity of meat in the diet. By contrast, sera tested from 479 persons living about 150 km to the east in the coastal, urban towns of Tehuantepec of Salina Cruz showed positive reactions among 122 (125.5%), and 71 (14.8%) had titers less than 1:1024. These people had a higher standard of living, more meat in their diet, and a few cats.</p>","PeriodicalId":75554,"journal":{"name":"Archivos de investigacion medica","volume":"22 1","pages":"63-73"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12981659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Campimetric alterations caused by pituitary microadenoma successfully treated by transsphenoidal adenomectomy.","authors":"I Madrazo-Navarro, J A Maldonado-León","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The authors are presenting the first description of the surgical treatment of an illness that is characterized by severe campimetric alterations (concentric reduction) in patients carrying prolactin-secreting microadenoma. Eleven demonstrative cases are presented.</p>","PeriodicalId":75554,"journal":{"name":"Archivos de investigacion medica","volume":"22 1","pages":"9-12"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12983030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R Román-Ramos, J L Flores-Sáenz, G Partida-Hernández, A Lara-Lemus, F Alarcón-Aguilar
{"title":"Experimental study of the hypoglycemic effect of some antidiabetic plants.","authors":"R Román-Ramos, J L Flores-Sáenz, G Partida-Hernández, A Lara-Lemus, F Alarcón-Aguilar","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The purpose of this work is to look for the hypoglycemic effect of 12 plants most used in Mexico for controlling diabetes mellitus. The studies were realized in 27 rabbits submitted weekly to glucose tolerance tests after gastric administration of water, tolbutamide or a preparation of the plant. The results showed that tolbutamide and studied plants (except Aloe barbadensis) decreased significantly (p less than 0.05) the area under glucose tolerance curve, in relation with the water control. The strongest effect was yielded by Psacalium peltatum (27.9%), followed by Curcubita ficifolia (26.4%), Lepechinia caulescens (26.0%), Opuntia streptacantha (21.4%), Slanum verbascifolum (21.1%), Teucrium cubense (19.4%), Cecropia obtusifolia (18.9%), Phaseolus vulgaris (18.5%), Tecoma stans (17.5%), Eriobotrya japonica (17.2%), Salpianthus macrodonthus (15.0%), tolbutamide (14.3%), and Aloe barbadensis (1.4%). Our results point out that the majority of the plants most used by the Mexican population to control diabetes mellitus have an evident hypoglycemic action.</p>","PeriodicalId":75554,"journal":{"name":"Archivos de investigacion medica","volume":"22 1","pages":"87-93"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12983029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A C Frati, B E Gordillo, P Altamirano, C R Ariza, R Cortés-Franco, A Chávez-Negrete, S Islas-Andrade
{"title":"Influence of nopal intake upon fasting glycemia in type II diabetics and healthy subjects.","authors":"A C Frati, B E Gordillo, P Altamirano, C R Ariza, R Cortés-Franco, A Chávez-Negrete, S Islas-Andrade","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>To assess if the acute hypoglycemic effect of nopal which occurs in diabetic patients also appears in healthy individuals, 500 g of nopal stems (O. streptacantha Lem.) were given orally to 14 healthy volunteers and to 14 patients with NIDDM. Serum glucose and insulin levels were measured at 0, 60, 120 and 180 minutes after nopal ingestion. A control test was performed with the intake of 400 ml of water. The intake of nopal by the NIDDM group was followed by a significant reduction of serum glucose and insulin concentration reaching 40.8 + 4.6 mg/dl (n = 14) (mean+SEM) and 7.8 + 1.5 uU/ml (n = 7) less than basal value, respectively, at 180 minutes. (P less than 0.001 vs control test). No significant changes were noticed in the healthy group as compared with the control test (P greater than 0.05). Acute hypoglycemic effect of nopal was observed in patients with NIDDM but not in healthy subjects, thus the mechanisms of this effect differs from current hypoglycemic agents.</p>","PeriodicalId":75554,"journal":{"name":"Archivos de investigacion medica","volume":"22 1","pages":"51-6"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12834190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Colonization by Clostridium difficile in hospitalized children: risk factors and typification of the isolated strains.","authors":"M Camorlinga, O Muñoz, H Guiscafré, J Torres","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The frequency of colonization by Clostridium difficile in 273 hospitalized children under 15 years of age was studied. Feces were collected from patients attending the infectious disease service at the Pediatric Hospital IMSS, during a period of 11 months. No colonization was detected in 16 neonates; whereas 10 of 103 children (9.7%) under one year of age, 7 of 84 children (8.3%) from one to five years and 3 of 70 children (4.2%) from five to 15 years of age were colonized. The use of antibiotics and the nutritional state were studied as possible risk factors for colonization. The frequency of colonization was not influenced by the nutritional state, whereas the treatment with antibiotics decreased significantly the colonization in children under one year of age but not in those children over one year of age. In children under one year of age, the cytotoxin was more frequent in cases of diarrhea, and in those over one year no association was found. The 50 strains isolated from these children were classified according to: toxigenicity, sensitivity to antibiotics, phages and bacteriocins. Strains acquired before hospitalization were more toxigenic than those acquired intrahospital. Twelve resistotypes were detected; one of them (V) was more frequent in intrahospital strains. Ten phagobacteriocin types were found, and two of then (D and I) were present only in intrahospital strains. Using this classification scheme, it was found that eight patients were colonized with two different strains at the same time.</p>","PeriodicalId":75554,"journal":{"name":"Archivos de investigacion medica","volume":"22 1","pages":"19-26"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12981653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
F Castañeda-Ibarra, L Ruíz-Maya, R Campos-Rodríguez, E García Latorre
{"title":"Polyclonal activation of B lymphocytes in patients with amoebic hepatic abscess.","authors":"F Castañeda-Ibarra, L Ruíz-Maya, R Campos-Rodríguez, E García Latorre","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In the course of tissue invasion by the amoeba, cellular immune responses are generated with the production of lymphokines by T lymphocytes that could induce activation of B cells. Therefore, it is possible that besides the induction of B lymphocytes specific for amoebic antigens, there could be activation of B cells with different specificities, that is, polyclonal in nature. IgM antibodies against some natural and synthetic antigens were quantified in both sera from amoebic hepatic abscess (AHA) patients and healthy controls by means of an ELISA. Titers in each case analyzed were significantly higher in AHA patients than in controls. Results indicate that there is polyclonal activation of B lymphocytes in this disease.</p>","PeriodicalId":75554,"journal":{"name":"Archivos de investigacion medica","volume":"22 1","pages":"13-7"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12981709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A Avilés, J C Díaz-Maqueo, E L García, V Torras, D López-Vancell
{"title":"Randomized study for the treatment of advanced Hodgkin's disease: MOPP vs. LOPP.","authors":"A Avilés, J C Díaz-Maqueo, E L García, V Torras, D López-Vancell","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Between January 1983 and December 1984, 83 patients with advanced Hodgkin's disease were entered in a prospective randomized trial comparing MOPP (mechlorethamine, vincristine, procarbazine and prednisone) with a regimen containing chlorambucil (Leukeran), vincristine, prednisone and procarbazine (LOPP). Both groups were comparable for the variables of age, stage, symptoms, histology and sites of involvement. Seventy percent of LOPP-treated patients achieved a complete remission compared to 65% of the MOPP-treated group. After a median follow-up of greater than 48 months, there is no statistical difference between the two treatment groups in survival or relapse free-survival. The LOPP combination was better tolerated with significantly less side effects. The drug regimen LOPP appears to be as effective as MOPP in producing complete remission and long term survival in patients with advanced Hodgkin's disease.</p>","PeriodicalId":75554,"journal":{"name":"Archivos de investigacion medica","volume":"22 1","pages":"45-50"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12981657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Changes in manganese and small cobalt blood levels of lead-exposed workers.","authors":"J Saavedra-Contreras, C Ríos","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Blood levels of manganese and cobalt were analyzed with graphite furnace atomic absorption spectrophotometry in three groups of workers: non-exposed, middle-exposed and heavy-exposed to lead from five lead-acid battery factories located in Mexico City. Manganese levels were significantly higher in the heavy-exposed workers than in the non-exposed subjects. Cobalt in blood concentrations was found to be lowered in both middle and heavy exposed workers as compared to the control group. The significance of these findings is discussed in terms of the possible mechanisms underlying these observations.</p>","PeriodicalId":75554,"journal":{"name":"Archivos de investigacion medica","volume":"22 1","pages":"57-61"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12981658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}