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Intracellular signaling transduction in the differentiation of Trypanosoma cruzi: role of cAMP. 克氏锥虫细胞内信号转导的分化:cAMP的作用。
Archivos de biologia y medicina experimentales Pub Date : 1988-12-01
R Rangel-Aldao, F Triana, G Comach, T Abate, V Fernández, D McMahon-Pratt
{"title":"Intracellular signaling transduction in the differentiation of Trypanosoma cruzi: role of cAMP.","authors":"R Rangel-Aldao,&nbsp;F Triana,&nbsp;G Comach,&nbsp;T Abate,&nbsp;V Fernández,&nbsp;D McMahon-Pratt","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>We have studied the cell differentiation of Trypanosoma cruzi in an vitro system that allows the transformation of epimastigotes into metacyclic trypomastigotes. Intracellular cAMP levels of epimastigotes increased 3 fold prior to their differentiation into metacyclics where cAMP remained elevated 3.7 fold with respect to epimastigotes. We also observed a 3 fold increase in the specific activity of cAMP-binding of metacyclics crude homogenates. This activity resided in a cAMP-binding receptor protein (CARPT) which was different from the typical cAMP-binding subunits (RI and RII) of cAMP-dependent protein kinases, as shown by the use of polyclonal antibodies prepared against these two types of proteins. Anti-RI antibodies did not react with CARPT, and anti-RII antibodies gave a cross reaction with CARPT which was at least 1,000 fold less sensitive than the one shown by the homologous antigen. On Western blots CARPT displayed a major band with Mr = 87,000 instead of Mr = 56,000 for RII. These studies implicate that cAMP may act as a mediator of the cell differentiation of T. cruzi by a mechanism involving a novel type of cAMP-binding receptor.</p>","PeriodicalId":75552,"journal":{"name":"Archivos de biologia y medicina experimentales","volume":"21 3-4","pages":"403-8"},"PeriodicalIF":0.0,"publicationDate":"1988-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13992851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cyclin/PCNA is a cell cycle modulated nuclear protein with a role in DNA replication. 细胞周期蛋白/PCNA是一种细胞周期调节核蛋白，在DNA复制中发挥作用。
Archivos de biologia y medicina experimentales Pub Date : 1988-12-01
J E Celis, P Madsen, H V Nielsen, B Gesser, H H Rasmussen, D Cruger
{"title":"Cyclin/PCNA is a cell cycle modulated nuclear protein with a role in DNA replication.","authors":"J E Celis,&nbsp;P Madsen,&nbsp;H V Nielsen,&nbsp;B Gesser,&nbsp;H H Rasmussen,&nbsp;D Cruger","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Cyclin/PCNA (auxiliary protein of DNA polymerase delta) is a proliferation sensitive DNA replication protein whose synthesis is modulated during the cell cycle. All available information suggests that cyclin/PCNA may trigger DNA replication following the formation of pre-replicative complexes at the G1/S transition border.</p>","PeriodicalId":75552,"journal":{"name":"Archivos de biologia y medicina experimentales","volume":"21 3-4","pages":"417-21"},"PeriodicalIF":0.0,"publicationDate":"1988-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14045175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Sequence conservations in vertebrate homeo-box mRNAs. 脊椎动物同源盒状mrna的序列保守性。
Archivos de biologia y medicina experimentales Pub Date : 1988-12-01
E M De Robertis, T R Burglin, A Fritz, G Oliver, K Cho, C V Wright
{"title":"Sequence conservations in vertebrate homeo-box mRNAs.","authors":"E M De Robertis,&nbsp;T R Burglin,&nbsp;A Fritz,&nbsp;G Oliver,&nbsp;K Cho,&nbsp;C V Wright","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":75552,"journal":{"name":"Archivos de biologia y medicina experimentales","volume":"21 3-4","pages":"443-7"},"PeriodicalIF":0.0,"publicationDate":"1988-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14045176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Sociedad Chilena de Ciencias Fisiológicas. Balneario el Retiro (Quilpue), 29-30 April 1988. 3d annual meeting. Abstracts]. 智利生理科学学会。Balneario el Retiro (Quilpue)， 1988年4月29-30日。3d年会。Abstracts]。
Archivos de biologia y medicina experimentales Pub Date : 1988-12-01
{"title":"[Sociedad Chilena de Ciencias Fisiológicas. Balneario el Retiro (Quilpue), 29-30 April 1988. 3d annual meeting. Abstracts].","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":75552,"journal":{"name":"Archivos de biologia y medicina experimentales","volume":"21 3-4","pages":"R489-96"},"PeriodicalIF":0.0,"publicationDate":"1988-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14386134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Sección de Biología Celular, Sociedad de Biología de Chile. 2d annual meeting. 25-27 August 1988, Termas de Panimávida. Abstracts]. [智利生物学学会细胞生物学科。2d年会上。1988年8月25-27日，panimavida温泉。Abstracts]。
Archivos de biologia y medicina experimentales Pub Date : 1988-12-01
{"title":"[Sección de Biología Celular, Sociedad de Biología de Chile. 2d annual meeting. 25-27 August 1988, Termas de Panimávida. Abstracts].","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":75552,"journal":{"name":"Archivos de biologia y medicina experimentales","volume":"21 3-4","pages":"R531-7"},"PeriodicalIF":0.0,"publicationDate":"1988-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14386137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The Chilean Society of Reproduction and Development. 2nd annual meeting. La Serena, November 22-24, 1988. Abstracts]. 智利生殖与发展协会，第二届年会。1988年11月22日至24日，拉塞雷纳。摘要]。
Archivos de biologia y medicina experimentales Pub Date : 1988-11-01
{"title":"[The Chilean Society of Reproduction and Development. 2nd annual meeting. La Serena, November 22-24, 1988. Abstracts].","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":75552,"journal":{"name":"Archivos de biologia y medicina experimentales","volume":"21 2","pages":"R343-63"},"PeriodicalIF":0.0,"publicationDate":"1988-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14283034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The Society of Biology of Chile. 31st annual meeting. La Serena, November 23-26, 1988. Abstracts]. 智利生物学会。第31届年会。1988年11月23日至26日，La Serena摘要]。
Archivos de biologia y medicina experimentales Pub Date : 1988-11-01
{"title":"[The Society of Biology of Chile. 31st annual meeting. La Serena, November 23-26, 1988. Abstracts].","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":75552,"journal":{"name":"Archivos de biologia y medicina experimentales","volume":"21 2","pages":"R261-341"},"PeriodicalIF":0.0,"publicationDate":"1988-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14283031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Photoaffinity labeling of pyruvate kinase from rabbit muscle. 兔肌中丙酮酸激酶的光亲和标记。
Archivos de biologia y medicina experimentales Pub Date : 1988-06-01
S Bazaes, M Bosch, H J Schäfer, J Eyzaguirre
{"title":"Photoaffinity labeling of pyruvate kinase from rabbit muscle.","authors":"S Bazaes,&nbsp;M Bosch,&nbsp;H J Schäfer,&nbsp;J Eyzaguirre","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Several studies have been performed on the structure of muscle pyruvate kinase. X-ray diffraction has provided a three-dimensional picture of the active site, and chemical modification studies have revealed essential amino acid residues for substrate binding or catalysis. We have shown that 8-azido-ADP (N3 ADP) behaves as a photoaffinity label for the enzyme. This reagent upon irradiation produces inactivation of the enzyme, and the activity loss is protected by nucleotides. The partially modified enzyme shows the same Km for ADP as the native one suggesting an \"all or none\" inactivation effect. The incorporation of 1 mole of 14C-N3 ADP per subunit correlates with complete inactivation. A radioactive peptide was isolated from the enzyme labeled with 14C-N3 ADP. The partial sequence of this peptide showed that it corresponds to the same peptide isolated from rabbit muscle pyruvate kinase labeled with dialdehyde-ADP and with trinitrobenzenesulfonate. This peptide is identical to a region in the cat and chicken muscle enzymes, and also a high degree of homology is found in a region of the rat liver and yeast enzymes. These studies show that N3 ADP binds to the same site as dialdehyde-ADP in rabbit muscle pyruvate kinase, and this site seems to be the nucleotide binding site.</p>","PeriodicalId":75552,"journal":{"name":"Archivos de biologia y medicina experimentales","volume":"21 1","pages":"123-7"},"PeriodicalIF":0.0,"publicationDate":"1988-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14114349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Proteolysis of mitochondrial-coded and nuclear-coded proteins found in yeast mitochondria. 酵母线粒体中发现的线粒体编码蛋白和核编码蛋白的蛋白质水解。
Archivos de biologia y medicina experimentales Pub Date : 1988-06-01
G Jacob, R Téllez, W Torres, R Ocasio, C Basilio, C George-Nascimento
{"title":"Proteolysis of mitochondrial-coded and nuclear-coded proteins found in yeast mitochondria.","authors":"G Jacob,&nbsp;R Téllez,&nbsp;W Torres,&nbsp;R Ocasio,&nbsp;C Basilio,&nbsp;C George-Nascimento","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The rate of degradation of radioactive labeled mitochondrial proteins synthesized both in vitro and in vivo by isolated yeast mitochondria and growing yeast cells respectively, has been studied. It was found that the in vitro-synthesized mitochondrial proteins are rapidly degraded by an energy-dependent proteolytic system. Under the same experimental conditions the in vivo-synthesized mitochondrial proteins are slowly degraded to a limited extent by a protease which is slightly inhibited by ATP. During this period, the mitochondria are coupled and metabolically active. It is proposed that mitochondria possess an energy-dependent proteolytic system that recognizes as substrates either \"abnormal\" proteins or unassembled protein subunits encoded in the mitochondrial genome. An apparently different system, which is independent of energy, seems to be responsible for the slow and limited degradation of \"normal\" mitochondrial proteins.</p>","PeriodicalId":75552,"journal":{"name":"Archivos de biologia y medicina experimentales","volume":"21 1","pages":"145-50"},"PeriodicalIF":0.0,"publicationDate":"1988-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14114350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The relationship between the structures of four beta-lactamases obtained from Bacillus cereus. 蜡样芽孢杆菌中四种β -内酰胺酶的结构关系。
Archivos de biologia y medicina experimentales Pub Date : 1988-06-01
H Cid, O Carrillo, M Bunster, J Martínez, V Vargas
{"title":"The relationship between the structures of four beta-lactamases obtained from Bacillus cereus.","authors":"H Cid,&nbsp;O Carrillo,&nbsp;M Bunster,&nbsp;J Martínez,&nbsp;V Vargas","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Bacillus cereus has proved to be one of the most interesting microorganisms in the study of beta-lactamases. It secrets these enzymes very efficiently and, frequently, in multiple forms. Three different forms are produced by strain 569/H; mutant 5/B of the same microorganism is constitutive for the secretion of beta-lactamases I and II. The present study, based on secondary structure prediction by two independent methods, states the relationship among the structures of beta-lactamases I, II and III produced by B. cereus 569/H and beta-lactamase I from the strain 5/B of this microorganism. A strong similarity is also established for the enzyme type III of B. cereus and the enzyme type I produced by B. licheniformis which could have an evolutionary explanation. A structural analysis of the leader peptide regions of these enzymes by the method of Mohana and Argos is also reported.</p>","PeriodicalId":75552,"journal":{"name":"Archivos de biologia y medicina experimentales","volume":"21 1","pages":"101-7"},"PeriodicalIF":0.0,"publicationDate":"1988-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14285311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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