克氏锥虫细胞内信号转导的分化:cAMP的作用。

R Rangel-Aldao, F Triana, G Comach, T Abate, V Fernández, D McMahon-Pratt
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引用次数: 0

摘要

我们在体外系统中研究了克氏锥虫的细胞分化,该系统允许将附属体转化为亚环型锥虫。在分化为元循环之前,细胞内的cAMP水平增加了3倍,其中cAMP保持在3.7倍的水平。我们还观察到元环粗匀浆的camp结合比活性增加了3倍。这种活性存在于camp结合受体蛋白(CARPT)中,该蛋白不同于camp依赖蛋白激酶的典型camp结合亚基(RI和RII),这一点可以通过制备针对这两种蛋白的多克隆抗体来证明。抗ri抗体与CARPT不发生反应,抗rii抗体与CARPT发生交叉反应,其敏感性至少比同源抗原低1000倍。在Western blots上,CARPT显示RII的Mr = 87,000,而不是Mr = 56,000。这些研究提示cAMP可能通过一种新型cAMP结合受体的机制作为克氏t细胞分化的介质。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Intracellular signaling transduction in the differentiation of Trypanosoma cruzi: role of cAMP.

We have studied the cell differentiation of Trypanosoma cruzi in an vitro system that allows the transformation of epimastigotes into metacyclic trypomastigotes. Intracellular cAMP levels of epimastigotes increased 3 fold prior to their differentiation into metacyclics where cAMP remained elevated 3.7 fold with respect to epimastigotes. We also observed a 3 fold increase in the specific activity of cAMP-binding of metacyclics crude homogenates. This activity resided in a cAMP-binding receptor protein (CARPT) which was different from the typical cAMP-binding subunits (RI and RII) of cAMP-dependent protein kinases, as shown by the use of polyclonal antibodies prepared against these two types of proteins. Anti-RI antibodies did not react with CARPT, and anti-RII antibodies gave a cross reaction with CARPT which was at least 1,000 fold less sensitive than the one shown by the homologous antigen. On Western blots CARPT displayed a major band with Mr = 87,000 instead of Mr = 56,000 for RII. These studies implicate that cAMP may act as a mediator of the cell differentiation of T. cruzi by a mechanism involving a novel type of cAMP-binding receptor.

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