结构和非结构多肽参与轮状病毒RNA合成。

A M Sandino, J Pizarro, J Fernández, M C Fellay, E Spencer
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引用次数: 0

摘要

轮状病毒是分节双链RNA病毒,在中心核周围有双蛋白衣壳。病毒在细胞质中复制。感染后,从病毒基因组中转录出11个mrna。为了进一步表征感染周期,利用标记前体研究了病毒多肽合成和RNA复制。非结构多肽NS34和NS35的参与是由病毒多肽在感染细胞中出现的动力学决定的。使用环己亚胺的实验表明,这两种多肽的合成都需要开始RNA复制。感染后8小时亚病毒颗粒的分离表明,存在一个含有非结构多肽和结构多肽VP1、VP2和VP6的颗粒,似乎能够转录病毒基因组以产生其他RNA物种。结果表明,存在一种类似于体外获得的核样颗粒,在加入VP6后能够转录病毒基因组。这似乎表明核样颗粒可能会根据与之相互作用的多肽改变其对正或负RNA合成的特异性。通过对病毒核和VP6产生抗体,分析了VP6与病毒核的相互作用。结果表明,VP6包含一个与核心复合体的特定结合位点,这种相互作用允许mRNA的合成。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Involvement of structural and nonstructural polypeptides on rotavirus RNA synthesis.

Rotavirus are segmented double stranded RNA viruses with a double protein capsid around a central core. The virus replicates in the cell cytoplasm. After infection, eleven mRNAs are transcribed from the viral genome. To characterize further the infection cycle, viral polypeptide synthesis and RNA replication were studied using labelled precursors. The involvement of nonstructural polypeptides NS34 and NS35 was determined by the kinetics of the appearance of viral polypeptides in infected cells. Experiments in which cycloheximide was used showed that the synthesis of both polypeptides was required to begin RNA replication. The isolation of subviral particles at 8 hours postinfection indicates that there is a particle containing the nonstructural polypeptides and the structural polypeptides VP1, VP2, and VP6 that seem to be able to transcribe the viral genome to produce other RNA species. The results suggest that there is a core-like particle similar to one obtained in vitro which upon the addition of VP6 is able to transcribe the virus genome. This seems to indicate that core-like particles may alter their specificity for plus or minus RNA synthesis depending upon the polypeptides that interact with it. The interaction between VP6 and the viral core was analyzed by means of antibodies raised against the viral core and VP6. The results suggest that VP6 contains a specific binding site to the core complex and this interaction allows the synthesis of mRNA.

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