Acta pathologica japonica最新文献

Mutations of the p53 gene in carcinomas of the urinary system. 泌尿系统癌中p53基因的突变。

Acta pathologica japonica Pub Date : 1993-12-01 DOI: 10.1111/j.1440-1827.1993.tb02561.x

Y Suzuki, G Tamura

{"title":"Mutations of the p53 gene in carcinomas of the urinary system.","authors":"Y Suzuki, G Tamura","doi":"10.1111/j.1440-1827.1993.tb02561.x","DOIUrl":"https://doi.org/10.1111/j.1440-1827.1993.tb02561.x","url":null,"abstract":"Deletion of p53, which is an anti-oncogene located on chromosome 17p, was reported to be present at a high incidence in tumor cells of colorectal carcinoma, as well as osteosarcoma of the familial cancer syndrome. Mutations of the p53 gene were investigated in 59 surgical specimens of primary carcinomas of the urinary system from 57 patients, using the polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) analysis. The PCR products were sequenced using the dideoxy chain termination method or the DNA sequencer. The tumors examined were 20 transitional cell carcinomas (TCC) and 39 renal cell carcinomas (RCC). Mutations of the p53 gene were detected in 20.0% (4/20) of TCC and were present in 16.7% (1/6) of the tumors invading the muscular layer. In two patients with simultaneous double bladder TCC, the mutations were found only in the larger tumors. In RCC, mutations were detected in 7.7% (3/39) of patients. No significant correlation between the presence of the mutation and the clinicopathologic parameters was found in RCC except that the three tumors with p53 gene mutations were clear cell carcinomas. These results suggest that p53 gene mutations play a possible role in both carcinogenesis and progression of TCC, but the p53 gene mutations may not be significant in development of RCC.","PeriodicalId":75413,"journal":{"name":"Acta pathologica japonica","volume":"43 12","pages":"745-50"},"PeriodicalIF":0.0,"publicationDate":"1993-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1440-1827.1993.tb02561.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19100771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 18

The numerical aberrations of chromosome 7 detected by fluorescence in situ hybridization in human breast cancers. 荧光原位杂交法检测乳腺癌患者7号染色体数值畸变。

Acta pathologica japonica Pub Date : 1993-12-01 DOI: 10.1111/j.1440-1827.1993.tb02559.x

N Yoshimi, C Shibuya, Y Morishita, T Tanaka, H Mori

{"title":"The numerical aberrations of chromosome 7 detected by fluorescence in situ hybridization in human breast cancers.","authors":"N Yoshimi, C Shibuya, Y Morishita, T Tanaka, H Mori","doi":"10.1111/j.1440-1827.1993.tb02559.x","DOIUrl":"https://doi.org/10.1111/j.1440-1827.1993.tb02559.x","url":null,"abstract":"The relationship between the numerical aberrations of chromosome 7 in interphase cells and the clinicopathological behavior of breast tumors was investigated in 51 touch imprinted preparations of breast tumors. Using fluorescence in situ hybridization with a chromosome 7-specific DNA probe, the fluorescein-isothiocyanate (FITC) spots mean and the representative copy number of each breast tumor were examined. The FITC spots mean (2.34) of 40 breast cancers increased compared with that of 11 benign lesions (1.98) (P < 0.02). The FITC spots mean tended to increase with the advancing stage and tumor size of the breast cancer. The FITC spots mean in the case with metastasis was also of a higher value than that without metastasis (P < 0.01). Furthermore, the existence of trisomy or over-trisomy of the copy number was related to the advancing stage and tumor size (P < 0.05 and P < 0.01, respectively). These findings suggest that the FITC spots mean and polysomy of the number of chromosome 7 may be highly predictive for breast tumor aggressiveness.","PeriodicalId":75413,"journal":{"name":"Acta pathologica japonica","volume":"43 12","pages":"730-5"},"PeriodicalIF":0.0,"publicationDate":"1993-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1440-1827.1993.tb02559.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19100770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

One step procedure of PCR-DNA extraction from paraffin-embedded materials by Chelex-100. 用Chelex-100从石蜡包埋材料中一步提取PCR-DNA。

Acta pathologica japonica Pub Date : 1993-12-01 DOI: 10.1111/j.1440-1827.1993.tb02568.x

N Yoshimi, M Suzuki, A Wang, T Kojima, H Mori

{"title":"One step procedure of PCR-DNA extraction from paraffin-embedded materials by Chelex-100.","authors":"N Yoshimi, M Suzuki, A Wang, T Kojima, H Mori","doi":"10.1111/j.1440-1827.1993.tb02568.x","DOIUrl":"https://doi.org/10.1111/j.1440-1827.1993.tb02568.x","url":null,"abstract":"The polymerase chain reaction (PCR) DNA amplification method is a powerful tool for the retrospective analysis of formalin-fixed and paraffin-embedded tissues.'-4 To utilize DNA from paraffin-embedded materials, several steps are required and may include transfers of DNA extracts to another container. In particular, the steps by xylene and ethanol need to remove paraffin from tissues for paraffin-embedded materials. These additional steps allow an increase in opportunities for cross-transfer of samples. Recently, it was reported that Chelexa-100 (Chelex; Bio-Rad, Richmond, VA, USA), which is a chelating resin and supplied commercially for the analysis of trace elements contained in soil, was used as a means of increasing the signal from the PCR amplification of small numbers of tissue culture cells and simplifying the pr~cedure.~ Stein and Raoult also reported the use of Chelex for paraffin-embedded tissues to detect bacterial DNA.6 Chelex was examined to detect ras oncogene in a small piece of biopsy. Samples used small pieces of 10 gastric biopsies from 10 patients with group Ill (so-called gastric adenoma) and were examined in the First Department of Pathology, Gifu University. All samples were fixed by 10% formalin and embedded conventionally in paraffin. The surface area of each sample was approximately 3-5mm2. All samples were cut from paraffin-embedded blocks into two sections of 6 pm thickness and then transferred to 1.5 mL microtubes. These were treated by three different procedures described as follows: first, as a conventional method, sections were deparaffinized with sequential washes of xylene and ethanol as described in previous dried and 100 pL of buffer (50 mmol/L","PeriodicalId":75413,"journal":{"name":"Acta pathologica japonica","volume":"43 12","pages":"790-1"},"PeriodicalIF":0.0,"publicationDate":"1993-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1440-1827.1993.tb02568.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19102153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Segmental glomerular necrosis as an active index of IgA nephropathy: a study with 100 serial sections of 128 cases. 节段性肾小球坏死作为IgA肾病的活动性指标:128例100个连续切片的研究

Acta pathologica japonica Pub Date : 1993-12-01 DOI: 10.1111/j.1440-1827.1993.tb02558.x

Y Shouno, T Shimokama, T Sakemi, A Harada, M Yamaguchi, T Watanabe

{"title":"Segmental glomerular necrosis as an active index of IgA nephropathy: a study with 100 serial sections of 128 cases.","authors":"Y Shouno, T Shimokama, T Sakemi, A Harada, M Yamaguchi, T Watanabe","doi":"10.1111/j.1440-1827.1993.tb02558.x","DOIUrl":"https://doi.org/10.1111/j.1440-1827.1993.tb02558.x","url":null,"abstract":"One hundred serial sections from each of 128 cases with immunoglobulin A (IgA) nephropathy were examined by light microscopy to clarify the relationship between segmental glomerular necrosis (SGN) and progression of glomerular injury. The cases were divided into five groups according to the percentage of glomeruli with cellular/fibrocellular (C/F) crescents, fibrous adhesion and/or sclerosis: grade I, 0%; grade II, < 20%; grade III, 20-50%; grade IV, 50-80%; and grade V, 80% or more. The serial sections revealed unequivocally focal occurrence of SGN in 39 cases (30%). Segmental glomerular necrosis was never found in the cases of grade I (0%, 0/28), while it appeared in those of grade II (33%, 12/36), grade III (46%, 13/28), grade IV (48%, 13/27) and grade V (11%, 1/9). The incidence of the cases with C/F crescents showed a similar tendency among the groups. In addition, focal C/F crescents were more frequent in cases with SGN (82%) than in those without SGN (24%). In particular, cellular crescents in 26 cases were formed in close proximity to SGN. These results suggested that SGN in IgA nephropathy was a more common finding than formerly evaluated and that it potentially participated in the progression of glomerular injuries closely associated with crescent formation. Unequivocally, focal occurrence of SGN corresponded well with the slowly progressive course of the glomerular disease.","PeriodicalId":75413,"journal":{"name":"Acta pathologica japonica","volume":"43 12","pages":"723-9"},"PeriodicalIF":0.0,"publicationDate":"1993-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1440-1827.1993.tb02558.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19100769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

Polymorphous low-grade adenocarcinoma of submandibular gland origin. 源自下颌骨腺的多形性低级别腺癌。

Acta pathologica japonica Pub Date : 1993-12-01 DOI: 10.1111/j.1440-1827.1993.tb02565.x

R Haba, S Kobayashi, H Miki, E Hirakawa, K Saoo, T Iwai, S Mori, M Ohmori

引用次数: 30

Immunohistochemical comparison between anaplastic seminoma and typical seminoma. 间变性精原细胞瘤与典型精原细胞瘤的免疫组化比较。

Acta pathologica japonica Pub Date : 1993-12-01 DOI: 10.1111/j.1440-1827.1993.tb02562.x

T Suzuki, H Sasano, H Aoki, H Nagura, N Sasano, T Sano, M Saito, T Watanuki, H Kato, S Aizawa

{"title":"Immunohistochemical comparison between anaplastic seminoma and typical seminoma.","authors":"T Suzuki, H Sasano, H Aoki, H Nagura, N Sasano, T Sano, M Saito, T Watanuki, H Kato, S Aizawa","doi":"10.1111/j.1440-1827.1993.tb02562.x","DOIUrl":"https://doi.org/10.1111/j.1440-1827.1993.tb02562.x","url":null,"abstract":"In order to study the possible biological differences between anaplastic and typical seminoma, the following factors were studied in 11 cases of anaplastic seminoma and 15 cases of typical seminoma: mitotic activity, proliferating cell nuclear antigen (PCNA) expression, immunohistochemical analyses for cytokeratin, vimentin, placental alkaline phosphatase (PLAP), beta-human chorionic gonadotropin (beta-hCG), alpha-fetoprotein (AFP) and c-myc oncoprotein. Anaplastic seminoma was classified according to Mostofi's criteria, which is primarily based on the mitotic activity of the tumor. Mitotic activity was evaluated by both mitotic count and rate. Statistically significant correlations were observed between mitotic count and mitotic rate (R = 0.891), and between the mitotic count and PCNA labeling index (R = 0.792), in both typical and anaplastic seminomas. Immunostaining patterns for cytokeratin, vimentin, PLAP, beta-hCG, AFP and c-myc oncoprotein were not significantly different between typical and anaplastic seminoma. The present data indicated that no apparent clinicopathologic and immunohistochemical parameters discerning anaplastic seminoma from typical seminoma were present, when identifying anaplastic seminoma on the basis of high mitotic count. Anaplastic seminoma may therefore simply represent seminoma with high proliferative activity.","PeriodicalId":75413,"journal":{"name":"Acta pathologica japonica","volume":"43 12","pages":"751-7"},"PeriodicalIF":0.0,"publicationDate":"1993-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1440-1827.1993.tb02562.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18517264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Histogenesis of abnormal elastic fibers in blebs and bullae of patients with spontaneous pneumothorax: ultrastructural and immunohistochemical studies. 自发性气胸患者大泡和大泡中异常弹性纤维的组织发生:超微结构和免疫组织化学研究。

Acta pathologica japonica Pub Date : 1993-12-01 DOI: 10.1111/j.1440-1827.1993.tb02557.x

S Haraguchi, Y Fukuda

{"title":"Histogenesis of abnormal elastic fibers in blebs and bullae of patients with spontaneous pneumothorax: ultrastructural and immunohistochemical studies.","authors":"S Haraguchi, Y Fukuda","doi":"10.1111/j.1440-1827.1993.tb02557.x","DOIUrl":"https://doi.org/10.1111/j.1440-1827.1993.tb02557.x","url":null,"abstract":"Elastic fibers in 15 blebs and 17 bullae with spontaneous pneumothorax were studied by means of electron microscopy and light and electron microscopic immunohistochemistry for elastin and alpha 1-antitrypsin. Blebs were formed in association with focal organized alveoli, and bullae were formed in association with pulmonary emphysema. Both blebs and bullae had abnormal elastic fibers. Ultrastructurally, abnormal elastic fibers of blebs and bullae consisted of accumulated thick and fine fibers. Accumulated thick elastic fibers showed vacuolar changes and electron-dense granular deposits, and they were associated with spiraling collagen fibrils. These thick elastic fibers reacted evenly with antielastin antibody and also reacted with anti-alpha 1-antitrypsin antibody. They are thought to be degraded elastic fibers. Accumulated fine elastic fibers consisted of bundles of microfibrils and granular amorphous components, and they reacted with anti-elastin and anti-alpha 1-antitrypsin antibody. These fine elastic fibers are thought to be not only newly formed in the process of organization but also degraded. It is suggested that elastic fibers of blebs and bullae are degraded due to an imbalance between elastase and alpha 1-antitrypsin.","PeriodicalId":75413,"journal":{"name":"Acta pathologica japonica","volume":"43 12","pages":"709-22"},"PeriodicalIF":0.0,"publicationDate":"1993-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1440-1827.1993.tb02557.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19100768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Distribution of laminin, type IV collagen and fibronectin in the invasive component of breast carcinoma. 层粘连蛋白、IV型胶原和纤维连接蛋白在乳腺癌浸润成分中的分布。

Acta pathologica japonica Pub Date : 1993-12-01 DOI: 10.1111/j.1440-1827.1993.tb02563.x

K Arihiro, K Inai, K Kurihara, S Takeda, M Kaneko

{"title":"Distribution of laminin, type IV collagen and fibronectin in the invasive component of breast carcinoma.","authors":"K Arihiro, K Inai, K Kurihara, S Takeda, M Kaneko","doi":"10.1111/j.1440-1827.1993.tb02563.x","DOIUrl":"https://doi.org/10.1111/j.1440-1827.1993.tb02563.x","url":null,"abstract":"Laminin, type IV collagen and fibronectin were examined immunohistochemically in the invasive component of breast carcinomas. Laminin was expressed around the invasive carcinoma cell nests in 38 (54%) of 71 cases. Immunoreactivity for type IV collagen was observed around the invasive carcinoma cell nests or the stroma apart from carcinoma cells in 44 (80%) of 55 cases. Fibronectin was strongly expressed in the stroma only in 75 (99%) of 76 cases. The expression of laminin significantly correlated with tubular formation in the invasive carcinoma cell nests and showed a tendency to be correlative to estrogen receptor (ER) and progesterone receptor (PgR) of carcinoma tissue, but no correlation among laminin expression, histological type, the age of patients, tumor size and lymph node metastasis was noted. Type IV collagen and fibronectin did not correlate to any clinicopathological factors such as histological type, grade of differentiation, the age of patients, tumor size, lymph node metastasis, ER and PgR status. No concordant expression of these extracellular matrices was seen.","PeriodicalId":75413,"journal":{"name":"Acta pathologica japonica","volume":"43 12","pages":"758-64"},"PeriodicalIF":0.0,"publicationDate":"1993-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1440-1827.1993.tb02563.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19100772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Two cases of recurrent hepatic injury associated with early pregnancy: hCG on hepatocytes is a suspected target antigen for lymphocyte attack. 两例与早孕相关的复发性肝损伤:肝细胞上的hCG被怀疑是淋巴细胞攻击的靶抗原。

Acta pathologica japonica Pub Date : 1993-12-01 DOI: 10.1111/j.1440-1827.1993.tb02564.x

K Miura, H Shirasawa, S Nagata, F Komoda, T Nakajima, K Kanai

{"title":"Two cases of recurrent hepatic injury associated with early pregnancy: hCG on hepatocytes is a suspected target antigen for lymphocyte attack.","authors":"K Miura, H Shirasawa, S Nagata, F Komoda, T Nakajima, K Kanai","doi":"10.1111/j.1440-1827.1993.tb02564.x","DOIUrl":"https://doi.org/10.1111/j.1440-1827.1993.tb02564.x","url":null,"abstract":"Two cases of recurrent hepatic injury which appeared in the first trimester of pregnancy were studied. Case 1 was a 35 year old woman, gravida 4, para 0, who suffered repeatedly from hepatic injury requiring induced abortions. The patient was healthy before the pregnancies and the plasma aminotransferases increased after 8 weeks gestation and promptly returned to normal after the abortions. No fluctuation of aminotransferases was observed in the menstrual cycle. A liver biopsy immediately after abortion showed spotty necrosis of hepatocytes with mononuclear cell infiltration. Most of the infiltrating cells were cytotoxic T cells that were directly in contact with hepatocytes. Numerous lymphocytic infiltrations were also found in the decidua of the uterine curettage material. The patient's lymphocytes showed conspicuous blast transformation in culture with human chorionic gonadotropin (hCG). The hCG was detected in close vicinity to the injured hepatocytes by immunostaining. Case 2 was a 23 year old woman, gravida 2, para 0, who underwent an induced abortion due to hepatic dysfunction in the first pregnancy. Although hepatic dysfunction reappeared from 10 weeks gestation during the second pregnancy, her health gradually improved with conservative therapy and resulted in a full-term delivery. She lacked allergies to drugs or foods and was healthy when she was not pregnant. These two cases suggest that some hepatotoxic materials appeared transiently in the first trimester. The results of Case 1 suggest strongly that hCG on the hepatocytes was recognized as an antigen and evoked lymphocytic attack.","PeriodicalId":75413,"journal":{"name":"Acta pathologica japonica","volume":"43 12","pages":"765-73"},"PeriodicalIF":0.0,"publicationDate":"1993-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1440-1827.1993.tb02564.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19100773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hepatocellular carcinoma with unusual metastasis to the small intestine. 肝细胞癌伴不寻常的小肠转移。

Acta pathologica japonica Pub Date : 1993-12-01 DOI: 10.1111/j.1440-1827.1993.tb02566.x

T Narita, H Nakazawa, Y Hizawa, T Ishidoya, H Kudo

引用次数: 4