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Correction to: Enhancement of doxorubicin production in Streptomyces peucetius by genetic engineering and process optimization. 更正:通过基因工程和工艺优化提高链霉菌生产多柔比星的能力。
IF 3.5 3区 生物学
AMB Express Pub Date : 2024-06-24 DOI: 10.1186/s13568-024-01724-1
Songbai Yang, Jiali Gui, Zhengyu Zhang, Jiawei Tang, Shaoxin Chen
{"title":"Correction to: Enhancement of doxorubicin production in Streptomyces peucetius by genetic engineering and process optimization.","authors":"Songbai Yang, Jiali Gui, Zhengyu Zhang, Jiawei Tang, Shaoxin Chen","doi":"10.1186/s13568-024-01724-1","DOIUrl":"10.1186/s13568-024-01724-1","url":null,"abstract":"","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"14 1","pages":"75"},"PeriodicalIF":3.5,"publicationDate":"2024-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11196429/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141441979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
New insight in molecular detection of Mycobacterium tuberculosis. 结核分枝杆菌分子检测的新见解。
IF 3.5 3区 生物学
AMB Express Pub Date : 2024-06-21 DOI: 10.1186/s13568-024-01730-3
Seyyed Mohammad Amin Mousavi-Sagharchi, Elina Afrazeh, Seyyedeh Fatemeh Seyyedian-Nikjeh, Maryam Meskini, Delaram Doroud, Seyed Davar Siadat
{"title":"New insight in molecular detection of Mycobacterium tuberculosis.","authors":"Seyyed Mohammad Amin Mousavi-Sagharchi, Elina Afrazeh, Seyyedeh Fatemeh Seyyedian-Nikjeh, Maryam Meskini, Delaram Doroud, Seyed Davar Siadat","doi":"10.1186/s13568-024-01730-3","DOIUrl":"10.1186/s13568-024-01730-3","url":null,"abstract":"<p><p>Mycobacterium tuberculosis, the causative agent of tuberculosis, is a pathogenic bacterium that has claimed millions of lives since the Middle Ages. According to the World Health Organization's report, tuberculosis ranks among the ten deadliest diseases worldwide. The presence of an extensive array of genes and diverse proteins within the cellular structure of this bacterium has provided us with a potent tool for diagnosis. While the culture method remains the gold standard for tuberculosis diagnosis, it is possible that molecular diagnostic methods, emphasis on the identification of mutation genes (e.g., rpoB and gyrA) and single nucleotide polymorphisms, could offer a safe and reliable alternative. Over the past few decades, as our understanding of molecular genetics has expanded, methods have been developed based on gene expansion and detection. These methods typically commence with DNA amplification through nucleic acid targeted techniques such as polymerase chain reaction. Various molecular compounds and diverse approaches have been employed in molecular assays. In this review, we endeavor to provide an overview of molecular assays for the diagnosis of tuberculosis with their properties (utilization, challenges, and functions). The ultimate goal is to explore the potential of replacing traditional bacterial methods with these advanced molecular diagnostic techniques.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"14 1","pages":"74"},"PeriodicalIF":3.5,"publicationDate":"2024-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11192714/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141436577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Antioxidant capacity of Pleurotus ostreatus (Jacq.) P. Kumm influenced by growth substrates. Pleurotus ostreatus (Jacq.) P. Kumm 的抗氧化能力受生长基质的影响。
IF 3.7 3区 生物学
AMB Express Pub Date : 2024-06-15 DOI: 10.1186/s13568-024-01698-0
Hailu Gebru, Gezahegn Faye, Tolosa Belete
{"title":"Antioxidant capacity of Pleurotus ostreatus (Jacq.) P. Kumm influenced by growth substrates.","authors":"Hailu Gebru, Gezahegn Faye, Tolosa Belete","doi":"10.1186/s13568-024-01698-0","DOIUrl":"10.1186/s13568-024-01698-0","url":null,"abstract":"<p><p>Functional constituents are the main concern in food production and consumption. Because foods rich in functional constituents have antioxidant capacity and are important in keeping consumers healthy. Pleurotus ostreatus is among foods rich in functional constituents. However, its functional constituents are affected by various factors. This study compared the antioxidant capacity of P. ostreatus grown on different substrates: straws of tef (Trt1), barley (Trt2), and wheat (Trt3), husks of faba bean (Trt4), and field pea (Trt5), sawdust (Trt6), and the mixture of the above with 1:1 w/w (Trt7). Trt7 had significantly higher radical scavenging activity (RSA) (73.27%), vitamin C (10.61 mg/100 g), and vitamin D (4.92 mg/100 g) compared to other treatments. Whereas the lowest values of RSA (44.24%), vitamin C (5.39 mg/100 g), and vitamin D (1.21 mg/100 g) were found in Trt2. The results indicated that mixed substrate may be a good growth substrate for functionally beneficial P. ostreatus and could be a promising source of natural antioxidants.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"14 1","pages":"73"},"PeriodicalIF":3.7,"publicationDate":"2024-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11180080/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141327099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Exploring a novel GH13_5 α-amylase from Jeotgalibacillus malaysiensis D5T for raw starch hydrolysis. 探索一种新型 GH13_5 α-淀粉酶(来自马来西亚 Jeotgalibacillus malaysiensis D5T),用于生淀粉水解。
IF 3.7 3区 生物学
AMB Express Pub Date : 2024-06-14 DOI: 10.1186/s13568-024-01722-3
Nurfatini Radzlin, Mohd Shukuri Mohamad Ali, Kian Mau Goh, Amira Suriaty Yaakop, Iffah Izzati Zakaria, Ummirul Mukminin Kahar
{"title":"Exploring a novel GH13_5 α-amylase from Jeotgalibacillus malaysiensis D5<sup>T</sup> for raw starch hydrolysis.","authors":"Nurfatini Radzlin, Mohd Shukuri Mohamad Ali, Kian Mau Goh, Amira Suriaty Yaakop, Iffah Izzati Zakaria, Ummirul Mukminin Kahar","doi":"10.1186/s13568-024-01722-3","DOIUrl":"10.1186/s13568-024-01722-3","url":null,"abstract":"<p><p>α-Amylase plays a crucial role in the industrial degradation of starch. The genus Jeotgalibacillus of the underexplored marine bacteria family Caryophanaceae has not been investigated in terms of α-amylase production. Herein, we report the comprehensive analysis of an α-amylase (AmyJM) from Jeotgalibacillus malaysiensis D5<sup>T</sup> (= DSM28777<sup>T</sup> = KCTC33550<sup>T</sup>). Protein phylogenetic analysis indicated that AmyJM belongs to glycoside hydrolase family 13 subfamily 5 (GH13_5) and exhibits low sequence identity with known α-amylases, with its closest counterpart being the GH13_5 α-amylase from Bacillus sp. KSM-K38 (51.05% identity). Purified AmyJM (molecular mass of 70 kDa) is stable at a pH range of 5.5-9.0 and optimally active at pH 7.5. The optimum temperature for AmyJM is 40 °C, where the enzyme is reasonably stable at this temperature. Similar to other α-amylases, the presence of CaCl<sub>2</sub> enhanced both the activity and stability of AmyJM. AmyJM exhibited activity toward raw and gelatinized forms of starches and related α-glucans, generating a mixture of reducing sugars, such as glucose, maltose, maltotriose, maltotetraose, and maltopentaose. In raw starch hydrolysis, AmyJM exhibited its highest efficiency (51.10% degradation) in hydrolyzing raw wheat starch after 3-h incubation at 40 °C. Under the same conditions, AmyJM also hydrolyzed tapioca, sago, potato, rice, and corn raw starches, yielding 16.01-30.05%. These findings highlight the potential of AmyJM as a biocatalyst for the saccharification of raw starches, particularly those derived from wheat.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"14 1","pages":"71"},"PeriodicalIF":3.7,"publicationDate":"2024-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11178733/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141316408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effectiveness of some plant extracts in biocontrol of induced onion basal rot disease in greenhouse conditions. 一些植物提取物在温室条件下生物防治洋葱基腐病的效果。
IF 3.7 3区 生物学
AMB Express Pub Date : 2024-06-14 DOI: 10.1186/s13568-024-01721-4
Mohamed G A Hegazy, Abdel-Raddy M Ahmed, Ahmed Fathy Yousef, Waleed M Ali, Alyaa Nasr, Ezzat H Elshazly, Mohamed E Shalaby, Islam I Teiba, Osama A M Al-Bedak
{"title":"Effectiveness of some plant extracts in biocontrol of induced onion basal rot disease in greenhouse conditions.","authors":"Mohamed G A Hegazy, Abdel-Raddy M Ahmed, Ahmed Fathy Yousef, Waleed M Ali, Alyaa Nasr, Ezzat H Elshazly, Mohamed E Shalaby, Islam I Teiba, Osama A M Al-Bedak","doi":"10.1186/s13568-024-01721-4","DOIUrl":"10.1186/s13568-024-01721-4","url":null,"abstract":"<p><p>One of Egypt's most notable and historically significant vegetable crops is the Liliaceae plant, Allium cepa L. In this study, the effectiveness of methanolic extracts of Artemisia absinthium leaves, Calotropis procera latex, Moringa oleifera seeds, and Syzygium aromaticum clove was investigated in vitro and, in a greenhouse, setting against Fusarium oxysporum, the pathogen that causes onion basal rot in Assiut Governorate, Egypt. The S. aromaticum extract exhibited the inhibition peak (63.3%), whereas the A. absinthium extract had the lowest inhibition impact against F. oxysporum growth (41.1%). The gas chromatography-mass spectroscopy (GC-MS) analysis revealed that 82 important compounds, with abundances ranging from low to high, were present in the tested S. aromaticum's methanolic extract. The primary components were acetaldehyde, hydroxy- and 2-propanone, 1,1,3,3-tetrachloro-(42.71%), 1,2-ethanediol, and methyl alcohol (34.01%). In comparison to the infected control, the disease severity was significantly reduced by 20% with the use of a plant extracts mixture and Dovex 50% and increased by 62.22% with the use of an extract from A. absinthium. When compared to the infected control, onion plant fresh weight and dry weight were considerably higher under the clove extract therapy. The plant extracts used in this study's testing contain a number of active ingredients, including amino acids, vitamins, minerals, antioxidants, and enzymes, which is probably why they have such positive impacts. The application of a combination of plant extracts was suggested as a feasible strategy for improving the growth and productivity of onion plants by the study's findings. More research is needed to comprehend the mechanisms by which plant extracts promote plant development and to optimize the concentration and timing of administration.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"14 1","pages":"72"},"PeriodicalIF":3.7,"publicationDate":"2024-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11178699/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141316407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A growth selection system for sucrose synthases (SuSy): design and test. 蔗糖合成酶(SuSy)生长选择系统:设计与测试。
IF 3.7 3区 生物学
AMB Express Pub Date : 2024-06-12 DOI: 10.1186/s13568-024-01727-y
Gonzalo N Bidart, Se Hyeuk, Tobias Benedikt Alter, Lei Yang, Ditte Hededam Welner
{"title":"A growth selection system for sucrose synthases (SuSy): design and test.","authors":"Gonzalo N Bidart, Se Hyeuk, Tobias Benedikt Alter, Lei Yang, Ditte Hededam Welner","doi":"10.1186/s13568-024-01727-y","DOIUrl":"10.1186/s13568-024-01727-y","url":null,"abstract":"<p><p>High throughput screening (HTS) methods of enzyme variants are essential for the development of robust biocatalysts suited for low impact, industrial scale, biobased synthesis of a myriad of compounds. However, for the majority of enzyme classes, current screening methods have limited throughput, or need expensive substrates in combination with sophisticated setups. Here, we present a straightforward, high throughput selection system that couples sucrose synthase activity to growth. Enabling high throughput screening of this enzyme class holds the potential to facilitate the creation of robust variants, which in turn can significantly impact the future of cost effective industrial glycosylation.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"14 1","pages":"70"},"PeriodicalIF":3.7,"publicationDate":"2024-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11169191/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141305215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Phenylalanine as an effective stabilizer and aggregation inhibitor of Bacillus amyloliquefaciens alpha-amylase. 苯丙氨酸作为淀粉芽孢杆菌α-淀粉酶的有效稳定剂和聚集抑制剂。
IF 3.7 3区 生物学
AMB Express Pub Date : 2024-06-08 DOI: 10.1186/s13568-024-01712-5
Leila Adibi, Parichehreh Yaghmaei, Parvaneh Maghami, Azadeh Ebrahim-Habibi
{"title":"Phenylalanine as an effective stabilizer and aggregation inhibitor of Bacillus amyloliquefaciens alpha-amylase.","authors":"Leila Adibi, Parichehreh Yaghmaei, Parvaneh Maghami, Azadeh Ebrahim-Habibi","doi":"10.1186/s13568-024-01712-5","DOIUrl":"10.1186/s13568-024-01712-5","url":null,"abstract":"<p><p>Aromatic compounds are known anti-amyloid aggregates. Their effect on amorphous aggregates of proteins is, however, less studied. We chose aromatic amino acids Trp, Tyr, and Phe, as well as another known stabilizer (i.e. Arg), as potential compatible solvents to be tested on Bacillus amyloliquefaciens alpha-amylase (BAA). Among these additives, Phe was the only one to be effective on the thermal inactivation and amorphous aggregation of BAA, while preserving its intrinsic activity. A concentration of 50 mM Phe was used to test its potential in counteracting the deleterious effect of BAA amorphous aggregates in vivo. After 21 days of daily subcutaneous injections of the native enzyme to mice, amorphous aggregates of BAA, as well as aggregates produced in presence of 50 mM Phe, the tissues located at the site of injection were studied histologically. Amorphous aggregates caused an increase in macrophages and lipid droplets. Serum levels of IL6 and TNF-α were also accordingly elevated and indicative of an inflammation state. Aggregates also resulted into increased levels of glucose, triglycerides and cholesterol, as well as liver enzymes SGOT and SGPT. On the other hand, the presence of Phe prevented this exacerbated inflammatory state and the subsequent impairment of biochemical parameters. In conclusion, Phe is an interesting compound for both stabilizing proteins and counteracting the pathological effect of amorphous aggregates.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"14 1","pages":"69"},"PeriodicalIF":3.7,"publicationDate":"2024-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11162409/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141293010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
An alternative method for SARS-CoV-2 detection with use modified fluorescent in situ hybridization. 使用改良荧光原位杂交法检测 SARS-CoV-2 的替代方法。
IF 3.7 3区 生物学
AMB Express Pub Date : 2024-06-06 DOI: 10.1186/s13568-024-01726-z
Agnieszka Sroka-Oleksiak, Agnieszka Krawczyk, Katarzyna Talaga-Ćwiertnia, Dominika Salamon, Monika Brzychczy-Włoch, Tomasz Gosiewski
{"title":"An alternative method for SARS-CoV-2 detection with use modified fluorescent in situ hybridization.","authors":"Agnieszka Sroka-Oleksiak, Agnieszka Krawczyk, Katarzyna Talaga-Ćwiertnia, Dominika Salamon, Monika Brzychczy-Włoch, Tomasz Gosiewski","doi":"10.1186/s13568-024-01726-z","DOIUrl":"10.1186/s13568-024-01726-z","url":null,"abstract":"<p><p>The real-time reverse-transcriptase polymerase-chain-reaction (rRT-PCR) tests are the gold standard in detecting SARS-CoV-2 virus infection. However, despite high sensitivity and specificity, they have limitations that in some cases may result in false negative results. Therefore, it is reasonable to search for additional tools that could support microbiological diagnosis of SARS-CoV-2. The aim of the study was to develop a highly specific molecular test capable of detecting and visualizing SARS-CoV-2 infection. A universal probe and a set of 18 specific oligonucleotides with a FLAP sequence attached to them on both sides were designed to visualize SARS-CoV-2 virus infection based on the fluorescence in situ hybridization method (FISH). FISH conditions using the developed kit were standardized on the Vero CCL-81 cell line infected by SARS-CoV-2 virus. The method was tested on 290 nasopharyngeal swabs (collected in a doublet) from patients with clinical symptoms of SARS-CoV-2. Each one swab from the doublet was subjected to RNA isolation and amplification by rRT-PCR. From the second swab, a microscopic preparation was performed for FISH. The use of the rRT-PCR allowed obtaining 200 positive and 90 negative results, while our FISH method allowed for 220 positive results and 70 negative results. The differences obtained using both methods were statistically significant (p = 0.008). The obtained results support the use of FISH as an additional method in microbiological diagnostics of SARS-CoV-2.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"14 1","pages":"64"},"PeriodicalIF":3.7,"publicationDate":"2024-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11156814/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141260700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Defluorination of monofluorinated alkane by Rhodococcus sp. NJF-7 isolated from soil. 从土壤中分离出来的罗得球菌 NJF-7 对单氟化烷的脱氟作用。
IF 3.7 3区 生物学
AMB Express Pub Date : 2024-06-06 DOI: 10.1186/s13568-024-01729-w
Meng Yan, Zhaozhao Gao, Xingjia Xiang, Qing Wang, Xin Song, Yucheng Wu, Frank E Löffler, Jun Zeng, Xiangui Lin
{"title":"Defluorination of monofluorinated alkane by Rhodococcus sp. NJF-7 isolated from soil.","authors":"Meng Yan, Zhaozhao Gao, Xingjia Xiang, Qing Wang, Xin Song, Yucheng Wu, Frank E Löffler, Jun Zeng, Xiangui Lin","doi":"10.1186/s13568-024-01729-w","DOIUrl":"10.1186/s13568-024-01729-w","url":null,"abstract":"<p><p>Microbial degradation of fluorinated compounds raised significant attention because of their widespread distribution and potential environmental impacts. Here, we report a bacterial isolate, Rhodococcus sp. NJF-7 capable of defluorinating monofluorinated medium-chain length alkanes. This isolate consumed 2.29 ± 0.13 mmol L<sup>- 1</sup> of 1-fluorodecane (FD) during a 52 h incubation period, resulting in a significant release of inorganic fluoride amounting to 2.16 ± 0.03 mmol L<sup>- 1</sup>. The defluorination process was strongly affected by the initial FD concentration and pH conditions, with lower pH increasing fluoride toxicity to bacterial cells and inhibiting enzymatic defluorination activity. Stoichiometric conversion of FD to fluoride was observed at neutral pH with resting cells, while defluorination was significantly lower at reduced pH (6.5). The discovery of the metabolites decanoic acid and methyl decanoate suggests that the initial attack by monooxygenases may be responsible for the biological defluorination of FD. The findings here provide new insights into microbial defluorination processes, specifically aiding in understanding the environmental fate of organic semi-fluorinated alkane chemicals.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"14 1","pages":"65"},"PeriodicalIF":3.7,"publicationDate":"2024-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11156826/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141260708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Microbial reduction of prebagged human plasma using 405 nm light and its effects on coagulation factors. 使用 405 纳米光对预包装人血浆进行微生物还原及其对凝血因子的影响。
IF 3.7 3区 生物学
AMB Express Pub Date : 2024-06-06 DOI: 10.1186/s13568-024-01725-0
Caitlin F Stewart, Preston McGoldrick, John G Anderson, Scott J MacGregor, Chintamani D Atreya, Michelle Maclean
{"title":"Microbial reduction of prebagged human plasma using 405 nm light and its effects on coagulation factors.","authors":"Caitlin F Stewart, Preston McGoldrick, John G Anderson, Scott J MacGregor, Chintamani D Atreya, Michelle Maclean","doi":"10.1186/s13568-024-01725-0","DOIUrl":"10.1186/s13568-024-01725-0","url":null,"abstract":"<p><p>Bacterial contamination is the most prevalent infectious complication of blood transfusion in the developed world. To mitigate this, several ultraviolet light-based pathogen reduction technologies (PRTs), some of which require photo-chemicals, have been developed to minimize infection transmission. Relative to UV light, visible 405-nm light is safer and has shown potential to be developed as a PRT for the in situ treatment of ex vivo human plasma and platelet concentrates, without the need for photo-chemicals. This study investigates the effect of 405-nm light on human plasma, with focus on the compatibility of antimicrobial light doses with essential plasma clotting factors. To determine an effective antimicrobial dose that is compatible with plasma, prebagged human plasma (up to 300 mL) was seeded with common microbial contaminants and treated with increasing doses of 405-nm light (16 mW cm<sup>-2</sup>; ≤ 403 J cm<sup>-2</sup>). Post-exposure plasma protein integrity was investigated using an AOPP assay, in vitro coagulation tests, and ELISA-based measurement of fibrinogen and Protein S. Microbial contamination in 300 mL prebagged human plasma was significantly reduced (P ≤ 0.05) after exposure to ≤ 288 J cm<sup>-2</sup>, with microbial loads reduced by > 96.2%. This dose did not significantly affect the plasma protein quality parameters tested (P > 0.05). Increased doses (≥ 345 J cm<sup>-2</sup>) resulted in a 4.3% increase in clot times with no statistically significant change in protein activity or levels. Overall, this study has demonstrated that the effective microbicidal 405 light dose shows little to no negative effect on plasma quality.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"14 1","pages":"66"},"PeriodicalIF":3.7,"publicationDate":"2024-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11156813/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141260793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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