AMB Express最新文献

{"title":"Repurposed bunamidine disrupts envelope energetics and induces ROS-mediated non-lytic killing.","authors":"Guanqing Huang, Shaowei Guo, Dan Xiao, Yiqing Liu, Mengna Li, Lihua Lu, Yelan Hong, Yimin Li, Mingxiang Zou, Yong Wu","doi":"10.1186/s13568-026-02016-6","DOIUrl":"10.1186/s13568-026-02016-6","url":null,"abstract":"<p><p>Methicillin-resistant Staphylococcus aureus (MRSA) remains a major public health threat due to its multidrug resistance, biofilm formation, and persistence, which collectively limit the effectiveness of current antibiotics. Here we characterize bunamidine hydrochloride (BUN), a repurposed benzimidazole derivative, as a potent anti-MRSA candidate. Across clinical and reference isolates, BUN displayed low MIC/MBC values (4–8 μg/mL), rapid bactericidal activity, and consistent efficacy in both planktonic and biofilm-associated populations. BUN eradicated persister cells within hours, while exhibiting a markedly reduced propensity for resistance development compared with ciprofloxacin. Mechanistic analyses revealed that BUN disrupts envelope energetics—causing membrane depolarization, collapse of the proton motive force, ATP depletion, and lipid disorder—without pore formation. These events triggered amplification of reactive oxygen species (ROS), which acted as critical execution factors for bacterial killing. Hemolysis assays confirmed selective compatibility with mammalian membranes, consistent with a non-lytic mode of action. In murine abscess and wound infection models, which represent early acute infection stages rather than established biofilm or chronic conditions, topical BUN treatment reduced bacterial burden, accelerated wound closure, and alleviated inflammation without apparent toxicity. These results demonstrate early therapeutic efficacy, tissue-protective, and anti-inflammatory effects in acute infection settings. While the current in vivo models support antibacterial efficacy during early infection, evaluation in established biofilm or chronic infection models will be an important direction for future studies.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":" ","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12965964/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146117513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nano-chitosan modified restorative materials suppress Streptococcus mutans biofilm and virulence gene expression.","authors":"Jakline N Saddik, Martha M Naguib, Labib M Labib, Ahmed O El-Gendy, Fatma Molham","doi":"10.1186/s13568-025-02004-2","DOIUrl":"10.1186/s13568-025-02004-2","url":null,"abstract":"<p><p>Secondary caries and biofilm production remain a persistent concern, prompting the development of antibacterial restorative materials. However, worries about synthetic additives’ safety and long-term effects urge the use of natural, biocompatible alternatives. This study explored the antibacterial efficacy of chitosan (CH) and nano-chitosan (NCH) incorporated into different restorative materials: amalgam (Am), glass ionomer cement (G), and resin composite (R). This study employs a direct comparative analysis of both modified and unmodified restorative materials against Am to evaluate their efficacy against Streptococcus mutans ATCC 25,175 using agar diffusion, broth inhibition, biofilm formation assays, and qPCR investigation of virulence genes (ldh and gtfB). Incorporating CH and NCH, especially at 15%, decreased biofilm biomass, downregulated virulence gene expression, and significantly increased the effectiveness of bacterial growth suppression. When compared to controls, the G modified with 15% NCH (GN15) showed the strongest inhibition of bacterial growth by 43.1%. The most significant finding was the ability of GN15 to downregulate the expression of ldh and gtfB by 50% and 28%, respectively, indicating a disruption in acid production and biofilm matrix synthesis. While Am showed the highest overall inhibition (86%, 65%). These results demonstrate that nano-chitosan is a biocompatible and promising addition to restorative materials, providing improved defense against cariogenic biofilms and facilitating the creation of dental biomaterials of the future.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":" ","pages":"20"},"PeriodicalIF":3.9,"publicationDate":"2026-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12920852/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146103596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial succession and machine learning for postmortem interval estimation in bromadiolone-poisoned cadavers.","authors":"Lipin Ren, Zhongjiang Wang, Zeyu Yang, Renmao Tian, Jinliang Du, Zhen Dang, Jianxia Li, Xinrui Guo, Yang Xia, Wen Cui, Yequan Wang","doi":"10.1186/s13568-026-02023-7","DOIUrl":"10.1186/s13568-026-02023-7","url":null,"abstract":"<p><p>Accurate estimation of the postmortem interval (PMI) remains a major challenge in forensic investigations. Although microbial succession has emerged as a promising biological clock, the impact of xenobiotic exposure on internal microbial communities during decomposition remain poorly understood. Here, we established a bromadiolone-poisoned rat model and systematically sampled gut and anus swabs at 6 h intervals over 96 h postmortem period. Bromadiolone exposure produced dose-dependent effects on decomposition, with high-doses delaying the progression, potentially by altering tissue integrity or microbial activity. 16 S rRNA sequencing revealed that Firmicutes and Proteobacteria were the dominant phyla during decomposition, followed by Bacteroidota, Desulfobacterota, and Actinobacteriota. Notably, microbial community dynamics were driven more strongly by anatomical location than by toxicant exposure, highlighting the critical role of sampling site. Furthermore, five machine-learning algorithms were evaluated for PMI prediction. GB regression outperformed RF, RR, SV, and LR, achieving an R² of 0.954 and an RMSE of 5.6 h. Feature analysis identified Proteus mirabilis and Morganella morganii as dominant positive biomarkers, while gut-associated taxa (e.g., Lactobacillus and Ligilactobacillus) declined over time. Together, these results demonstrate that bromadiolone exposure induces site-specific perturbations in microbial community, the underlying successional dynamics remain sufficiently robust to support accurate PMI estimation. The GB model provides both predictive accuracy and biological interpretability, highlighting the importance of incorporating anatomical location and toxicological stress into microbiome-based PMI estimation.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":" ","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12960868/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146112013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Agaricus arvensis extract-coated functionalized silver nanoparticles for effective aerobic biotransformation of synthetic L-tyrosine to L-DOPA.","authors":"Yan Li, Sikander Ali, Qaiser Farid Khan, Muhammad Usman Ahmad, Laraib Fatima, Tawaf Ali Shah, Molalign Assefa, Ahmed Al-Emam","doi":"10.1186/s13568-026-02013-9","DOIUrl":"10.1186/s13568-026-02013-9","url":null,"abstract":"<p><p>This study presents a green nanobiotechnological platform for enhanced L-DOPA biosynthesis. Intracellular tyrosine hydroxylase (TH) was immobilized onto silver nanoparticles (AgNPs) biosynthesized using extract of the edible mushroom Agaricus arvensis and subsequently applied for the aerobic biotransformation of synthetic L-tyrosine. The AgNPs were produced using the mushroom extract as a natural reducing agent and optimized for \"TH\" activity under controlled conditions: 2.5% (w/v) biomass concentration, peptone-saline medium, 90 °C, and 24 min reaction time. Various analytical techniques such as UV-Vis spectroscopy, SEM, FTIR and XRD were used to confirm the synthesis and characterization of the AgNPs. The particle size was further validated by Scanning Electron Microscopy (SEM), revealing an average size of 88.49 ± 3.83 nm. The particle size was further analyzed by a Zeta-sizer, which showed a Z-average size of 163.7 d.nm. When applied in the biotransformation of synthetic L-tyrosine, the AgNP-immobilized enzyme significantly outperformed the free enzyme, yielding a 2.54-fold increase in L-DOPA production within 1.5 h. These results indicate that Agaricus arvensis-derived AgNPs not only stabilize the enzyme but also boost its activity, providing a sustainable and efficient approach for producing L-DOPA. This method holds promise for large-scale applications in pharmaceutical manufacturing.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":" ","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12953818/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146099819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enzymatic preparation of epigallocatechin gallate diglucoside and bioactivity assessment.","authors":"Bohan Yang, Nianqing Zhu, Yuancheng Huang, Jianming Deng, Min Yu, Xiaodong Yan, Jing Wang","doi":"10.1186/s13568-026-02014-8","DOIUrl":"10.1186/s13568-026-02014-8","url":null,"abstract":"<p><p>Epigallocatechin gallate (EGCG), the predominant catechin in green tea, has limited application due to its poor water solubility and instability. To address these issues, this study utilized recombinant sucrose phosphorylase to catalyze the glucosylation of EGCG, successfully synthesizing (-)-epigallocatechin gallate 4',4″-O-α-D-diglucopyranoside (EGCG-2G). The process was optimized using response surface methodology, achieving a 97.46% conversion rate of EGCG. EGCG-2G was purified to ≥ 99% purity by semi-preparative liquid chromatography. It exhibited approximately 124-fold higher water solubility than EGCG and demonstrated significantly enhanced stability under thermal and acidic conditions (50 °C, pH = 5), with an 84.82% and 35.36% improvement over EGCG and commercial (-)-epigallocatechin-3-gallate-4'-O-α-D-glucoside (EGCG-1G), respectively. Furthermore, EGCG-2G displayed notable antioxidant, anti-inflammatory, and anti-melanogenic activities. It effectively scavenged intracellular and extracellular free radicals, reduced inflammatory cytokine levels, and inhibited melanin synthesis. Molecular docking and gene expression analyses suggested that its anti-melanogenic effect might be associated with the MC1R/cAMP/MITF signaling pathway.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":" ","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12953839/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146099887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: An effective method to enhance transient gene expression by transfecting high-density CHO cells.","authors":"Yu-Shun Fu, Xue-Lian Han, Wei-Dong Li, Lin-Lin Shan, Shao-Lei Geng, Chun-Liu Mi, Wei-Hua Dong, Tian-Yun Wang, Xiao-Yin Wang","doi":"10.1186/s13568-026-02010-y","DOIUrl":"10.1186/s13568-026-02010-y","url":null,"abstract":"","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":"16 1","pages":"8"},"PeriodicalIF":3.7,"publicationDate":"2026-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12858671/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146083806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Myeloperoxidase-DNA complex: a marker and combined target for Pseudomonas aeruginosa-associated bronchiectasis.","authors":"Shaochu Zheng, Jinling Tang, Xiaopu Wu, Cao Qing, Yun Jiang, Wei Lu, Chongxi Bao, Kangkang Hong, Jing Luo, Jinliang Kong","doi":"10.1186/s13568-026-02012-w","DOIUrl":"10.1186/s13568-026-02012-w","url":null,"abstract":"<p><p>Pseudomonas aeruginosa (P. aeruginosa)-associated bronchiectasis remains a clinical challenge due to lacking diagnostic biomarkers and targeted therapies. This multi-dimensional (clinical-cell-animal) study investigated the myeloperoxidase (MPO)-DNA complex, a marker of neutrophil extracellular traps (NETs), and the combination of AZD5904 (an MPO inhibitor) and DNase I (a NETs-DNA degrader). Key findings include: First, integrated evidence from bronchoalveolar lavage fluid analysis and immunofluorescence identified the MPO-DNA complex as a biomarker significantly associated with P. aeruginosa-associated bronchiectasis severity (e.g., lung lobe involvement, Bronchiectasis Severity Index). Mendelian randomization (MR) analysis revealed a potential causal link with bronchiectasis risk but not with several other chronic respiratory diseases. Second, in cellular models, P. aeruginosa PAO1-induced NETs were associated with epithelial damage, as evidenced by the upregulation of reactive oxygen species, malondialdehyde, interleukin-1β, and interleukin-6, and the reduction of BEAS-2B cell viability. Third, in P. aeruginosa-infected bronchiectasis rat models, AZD5904 combined with DNase I alleviated lung pathology, inflammation, and NETs accumulation more effectively than monotherapy. This study suggests the MPO-DNA complex as a potential biomarker and pathogenic factor associated with disease severity, and proposes a dual-target combined intervention strategy worthy of further preclinical investigation for P. aeruginosa-associated bronchiectasis.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":" ","pages":"17"},"PeriodicalIF":3.9,"publicationDate":"2026-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12909637/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146028011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Limosilactobacillus reuteri fermentation enhances the anti-type 2 diabetic activity of Gastrodia elata polysaccharides.","authors":"Jiajia Liu, Zongyang Li, Shixiao Pu, Xing Huang, Zixian Xu, Xiaoqi Yang, Yunlong Zhang, Chengying Luo, Shibo Li, Jiayun Miao, Yicen Lin, Lianbing Lin","doi":"10.1186/s13568-026-02008-6","DOIUrl":"10.1186/s13568-026-02008-6","url":null,"abstract":"<p><p>The development of effective and low-toxicity treatments for type 2 diabetes mellitus (T2DM) remains a critical objective in current research. Lactic acid bacteria (LAB) fermentation can biotransform plant polysaccharides and may enhance their bioactivity. Here, we investigated the fermentation with Limosilactobacillus reuteri DSM17938 fermentation affects the structure of Gastrodia elata polysaccharides and their potentail benefits in T2DM. Polysaccharides were extracted from non-fermented G. elata (GP) and from G. elata fermented with L. reuteri DSM 17,938 under controlled conditions to yield fermented polysaccharides (LGP). Fermentation reduced molecular weight and shifted monosaccharide composition, including increased content of galactose, arabinose, glucose, xylose, and galacturonic acid while decreased content of fructose. Fermentation reduced the molecular weight, altered the morphology, and shifted the monosaccharide composition, including increased content of arabinose and galacturonic acid while decreasing fructose. In a T2DM mouse model, LGP (10.9 ± 0.8 mmol/L) demonstrated a more significant hypoglycemic effect compared to GP (14.6 ± 1.4 mmol/L, p < 0.001). Furthermore, LGP more effectively improved antioxidant and inflammatory profiles markers (p < 0.001), and attenuated intestine and kidney injury. Mechanistic investigations revealed that LGP modulates gut microbiota composition by reducing the Firmicutes/Bacteroidetes ratio and promoting the proliferation of beneficial bacteria such as Kineothrix and Akkermansia, while concurrently increasing short-chain fatty acid levels and regulating serum metabolic pathways related to carbohydrate and protein digestion and absorption. This study demonstrates that LAB fermentation is an effective bioprocessing strategy for enhancing the hypoglycemic activity of GPs, offering novel insights for the production of functional foods.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":" ","pages":"16"},"PeriodicalIF":3.9,"publicationDate":"2026-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12894524/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145997126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lactiplantibacillus plantarum P16 mitigated murine colitis through modulation of immune response and gut microbiota.","authors":"Zhixian Chen, Qian Cheng, Xiaoli Zhang, Zhouhuang Sheng, Lei Shi, Yan Zhang","doi":"10.1186/s13568-025-02003-3","DOIUrl":"10.1186/s13568-025-02003-3","url":null,"abstract":"<p><p>Probiotics show potential for preventing and mitigating inflammatory bowel disease (IBD), though their efficacy and mechanisms are strain-specific. This study aimed to elucidate the protective mechanism of Lactiplantibacillus (L.) plantarum P16 against dextran sulfate sodium (DSS)-induced ulcerative colitis (UC). The strain exhibited high gastrointestinal tolerance, with survival rates of approximately 86% in artificial gastric fluid (pH 3.0) and 94% in 0.3% bile salt after 3 h of incubation. Moreover, L. plantarum P16 showed stronger adhesion to Caco-2 cells, indicating a high potential for intestinal colonization. In a DSS-induced colitis model, L. plantarum P16 administration alleviated colitis by reducing the disease activity index (DAI), suppressing pro-inflammatory cytokines (TNF-α, IL-6, IL-1β), and mitigating colonic tissue damage while preserving epithelial barrier integrity. Additionally, L. plantarum P16 treatment increased short-chain fatty acid (SCFA) levels. 16 S rRNA amplicon sequencing of cecal content revealed that L. plantarum P16 supplementation restored gut microbial homeostasis by elevating the abundances of Firmicutes and Verrucomicrobiota, particularly Akkermansia, while reducing the proportions of Bacteroides and Helicobacter. These findings demonstrated that L. plantarum P16 could alleviate colitis by ameliorating intestinal inflammation and restoring gut microbiota dysbiosis, proposing a novel perspective of L. plantarum P16 for UC management.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":" ","pages":"15"},"PeriodicalIF":3.7,"publicationDate":"2026-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12894483/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145987569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heterologous expression of Thermomyces lanuginosus lipase in Aspergillus niger: stress resistance, optimization of fermentation conditions, and transcriptomic analysis.","authors":"Xiaoyan Shang, Rong Tang, Nanyu Han, Zunxi Huang, Le Yang","doi":"10.1186/s13568-025-02007-z","DOIUrl":"10.1186/s13568-025-02007-z","url":null,"abstract":"<p><p>Aspergillus niger serves as a cell factory for efficient enzyme production. The lipase derived from Thermomyces lanuginosus (TLL) is known for its remarkable thermal stability and is extensively utilized in various industrial fields. In this study, a heterologous expression strain, ΔAnTll-11, of TLL was successfully constructed in A. niger. Through fermentation optimization, the lipase activity was enhanced 8.7-fold to 4547.95 U/mL, compared with the initial value of 520 U/mL. Enzymatic characterization indicated that the recombinant lipase exhibited optimum activity at pH 9.5 and 45 °C, and its activity was positively influenced by Ca²⁺, Ag⁺, Mg²⁺, and Cu²⁺. The ΔAnTll-11 strain exhibited enhanced tolerance to high osmolarity, oxidative stress, and thermal shock. Furthermore, transcriptomic analysis of the ΔAnTll-11 strain revealed that half of the total annotated genes (7199 genes) were differentially expressed genes (DEGs). According to protein‒protein interaction network and weighted gene coexpression network analyses, genes involved in ribosome function, amino acid metabolism, glycosylation, energy metabolism, and the MAPK signalling pathway may affect the expression of lipase. The transcriptomic findings elucidated the regulatory mechanisms by which A. niger expresses foreign proteins and enzymes, establishing a groundwork for further enhancing A. niger as a cell factory for efficient enzyme and protein production.</p>","PeriodicalId":7537,"journal":{"name":"AMB Express","volume":" ","pages":"14"},"PeriodicalIF":3.7,"publicationDate":"2026-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12873016/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145931758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}