Uirusu Pub Date : 2023-01-01 DOI: 10.2222/jsv.73.85

Yuta Tsukamoto, Manabu Igarashi, Hiroki Kato

引用次数: 0

[Neutralization of hepatitis B virus with vaccine-escape mutations by novel hepatitis B vaccine with large-HBs antigen]. [含大-HBs 抗原的新型乙型肝炎疫苗中和疫苗逃逸变异的乙型肝炎病毒]。

Uirusu Pub Date : 2023-01-01 DOI: 10.2222/jsv.72.149

Takanobu Kato, Hirofumi Akari

{"title":"[Neutralization of hepatitis B virus with vaccine-escape mutations by novel hepatitis B vaccine with large-HBs antigen].","authors":"Takanobu Kato, Hirofumi Akari","doi":"10.2222/jsv.72.149","DOIUrl":"10.2222/jsv.72.149","url":null,"abstract":"Although the current hepatitis B (HB) vaccine comprising yeast-derived small hepatitis B surface antigen (HBsAg) is potent and safe and used worldwide, specific concerns should not be ignored, such as the attenuated prophylaxis against hepatitis B virus (HBV) infection with specific amino acid polymorphisms, called vaccine-escape mutations (VEMs). We investigated a novel HB vaccine consisting of large-HBsAg that covers the shortcomings of the current HB vaccine in a nonhuman primate model. The yeast-derived large-HBsAg was mixed with the adjuvant and used to immunize rhesus macaques, and the induction of antibodies to HBsAg was compared with that of the current HB vaccine. The current HB vaccine predominantly induced antibodies to small-HBsAg, whereas immunization with the large-HBsAg vaccine mainly induced antibodies to the preS1 region. Although the antibodies induced by the current HB vaccine could not prevent infection of HBV with VEMs, the large-HBsAg vaccine-induced antibodies neutralized infection of HBV with VEMs at levels similar to those of the wild type. The HBV genotypes that exhibited attenuated neutralization by induced antibodies differed between these vaccines. In conclusion, the novel HB vaccine consisting of large-HBsAg was revealed to be useful to compensate for shortcomings of the current HB vaccine. The combined use of these HB vaccines may be able to induce antibodies that can neutralize HBV strains with VEMs or multiple HBV genotypes.","PeriodicalId":75275,"journal":{"name":"Uirusu","volume":"72 2","pages":"149-158"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139467425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Endogenous viral emelement limit cognate virus replication in mosquito vectors]. [内源性病毒元素限制蚊媒中同源病毒的复制]。

Uirusu Pub Date : 2023-01-01 DOI: 10.2222/jsv.72.159

Yasutsugu Suzuki

引用次数: 0

[Establishment of human sapovirus culture method]. [建立人类沙波病毒培养方法]。

Uirusu Pub Date : 2023-01-01 DOI: 10.2222/jsv.73.1

Hirotaka Takagi, Tomoichiro Oka

{"title":"[Establishment of human sapovirus culture method].","authors":"Hirotaka Takagi, Tomoichiro Oka","doi":"10.2222/jsv.73.1","DOIUrl":"https://doi.org/10.2222/jsv.73.1","url":null,"abstract":"More than 40 years after the discovery of human sapovirus (HuSaV), we have established a HuSaV culture system in which HuTu80 cells derived from the human duodenum adenocarcinoma cell line are cultured together with the addition of bile acid as a supplement. In addition to being a common cell line, this system using HuTu80 cells is a versatile method because classical culture media are available, and it is easy to scale-up for culture. However, the number of culture days required to obtain sufficient viral titer, the confirmation of viral gene conservation for sample selection, and the method for passaging of HuTu80-cells were crucial. So far, 15 genotypes have been successfully propagated and stocked, and stable supply as research resources has been achieved. Due to the above efforts, we can now proceed with the production and analysis of antisera using purified antigens and the evaluation of inactivation conditions. This manuscript introduces the background for selection of the cell line and bile acids, and the topics that have been discussed since the publication, as well as future issues that were raised such as the expression of cytopathicity and elucidation of low UV-C sensitivity of fecal-derived HuSaV.","PeriodicalId":75275,"journal":{"name":"Uirusu","volume":"73 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142334079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Structural insights into Hepatitis B and D virus entry receptors]. [乙型肝炎病毒和丁型肝炎病毒入口受体的结构研究]。

Uirusu Pub Date : 2023-01-01 DOI: 10.2222/jsv.73.89

Kanako Terakado Kimura, Koichi Watashi

引用次数: 0

Uirusu Pub Date : 2023-01-01 DOI: 10.2222/jsv.73.53

引用次数: 0

Uirusu Pub Date : 2023-01-01 DOI: 10.2222/jsv.73.77

引用次数: 0

Uirusu Pub Date : 2023-01-01 DOI: 10.2222/jsv.73.57

引用次数: 0

[Molecular epidemiology and evolution of human noroviruses]. [人类诺罗病毒的分子流行病学和进化]。