Uirusu最新文献_第2页

[In vitro propagation system for human norovirus]. [人类诺瓦克病毒体外繁殖系统]。

Uirusu Pub Date : 2023-01-01 DOI: 10.2222/jsv.73.9

Shintaro Sato

引用次数: 0

[SARS-CoV-2-specific T cell recognition toward emerging variants]. [SARS-CoV-2特异性T细胞识别新出现的变种]。

Uirusu Pub Date : 2023-01-01 DOI: 10.2222/jsv.73.173

Chihiro Motozono

{"title":"[SARS-CoV-2-specific T cell recognition toward emerging variants].","authors":"Chihiro Motozono","doi":"10.2222/jsv.73.173","DOIUrl":"https://doi.org/10.2222/jsv.73.173","url":null,"abstract":"Cytotoxic T lymphocytes (CTLs) play an important role in the control of various viral infection. CTLs recognize a complex of HLA (human leukocyte antigen) class I molecule and epitope peptide derived from viral protein on the cell surface via T cell receptors and can destroy virally infected cells. It is becoming evident that SARS-CoV-2 specific T cells play a crucial role in the control of COVID-19. We characterized T cells specific for various SARS-CoV-2 variants and identified that a L452R mutation in the Delta spike protein evades HLA-A*24:02-restricted T cell responses and increases virus infectivity. In contrast, HLA-A*24:02-restricted T cells strongly suppresses Omicron BA.1 replication due to a G446S mutation, located just outside the N-terminus of the cognate epitope, in the Omicron BA.1 variant via enhanced antigen processing and presentation of the epitope. These data indicate that T cell specific for antigens derived from variable regions is highly susceptible for the mutation and its location. The detail analysis of antigen-specific T cell responses toward variants provides better insights for the rational design of vaccine antigens or immunotherapy to induce efficient cellular immunity against new emerging viruses/variants.","PeriodicalId":75275,"journal":{"name":"Uirusu","volume":"73 2","pages":"173-182"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142334092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Uirusu Pub Date : 2023-01-01 DOI: 10.2222/jsv.73.75

引用次数: 0

Uirusu Pub Date : 2023-01-01 DOI: 10.2222/jsv.73.81

引用次数: 0

[Epidemiological and mechanistic links between Epstein-Barr virus and multiple sclerosis]. [Epidemiological and mechanistic links between Epstein-Barr virus and multiple sclerosis]（Epidemiological and mechanistic links between Epstein-Barr virus and multiple sclerosis）。

Uirusu Pub Date : 2023-01-01 DOI: 10.2222/jsv.73.147

Yoshitaka Sato

引用次数: 0

[Identification of anti-influenza A and B virus drugs targeting cellular methyltransferase]. [鉴定以细胞甲基转移酶为靶点的抗甲型和乙型流感病毒药物]。

Uirusu Pub Date : 2023-01-01 DOI: 10.2222/jsv.73.85

Yuta Tsukamoto, Manabu Igarashi, Hiroki Kato

引用次数: 0

[Endogenous viral emelement limit cognate virus replication in mosquito vectors]. [内源性病毒元素限制蚊媒中同源病毒的复制]。

Uirusu Pub Date : 2023-01-01 DOI: 10.2222/jsv.72.159

Yasutsugu Suzuki

引用次数: 0

[Neutralization of hepatitis B virus with vaccine-escape mutations by novel hepatitis B vaccine with large-HBs antigen]. [含大-HBs 抗原的新型乙型肝炎疫苗中和疫苗逃逸变异的乙型肝炎病毒]。

Uirusu Pub Date : 2023-01-01 DOI: 10.2222/jsv.72.149

Takanobu Kato, Hirofumi Akari

{"title":"[Neutralization of hepatitis B virus with vaccine-escape mutations by novel hepatitis B vaccine with large-HBs antigen].","authors":"Takanobu Kato, Hirofumi Akari","doi":"10.2222/jsv.72.149","DOIUrl":"10.2222/jsv.72.149","url":null,"abstract":"Although the current hepatitis B (HB) vaccine comprising yeast-derived small hepatitis B surface antigen (HBsAg) is potent and safe and used worldwide, specific concerns should not be ignored, such as the attenuated prophylaxis against hepatitis B virus (HBV) infection with specific amino acid polymorphisms, called vaccine-escape mutations (VEMs). We investigated a novel HB vaccine consisting of large-HBsAg that covers the shortcomings of the current HB vaccine in a nonhuman primate model. The yeast-derived large-HBsAg was mixed with the adjuvant and used to immunize rhesus macaques, and the induction of antibodies to HBsAg was compared with that of the current HB vaccine. The current HB vaccine predominantly induced antibodies to small-HBsAg, whereas immunization with the large-HBsAg vaccine mainly induced antibodies to the preS1 region. Although the antibodies induced by the current HB vaccine could not prevent infection of HBV with VEMs, the large-HBsAg vaccine-induced antibodies neutralized infection of HBV with VEMs at levels similar to those of the wild type. The HBV genotypes that exhibited attenuated neutralization by induced antibodies differed between these vaccines. In conclusion, the novel HB vaccine consisting of large-HBsAg was revealed to be useful to compensate for shortcomings of the current HB vaccine. The combined use of these HB vaccines may be able to induce antibodies that can neutralize HBV strains with VEMs or multiple HBV genotypes.","PeriodicalId":75275,"journal":{"name":"Uirusu","volume":"72 2","pages":"149-158"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139467425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Establishment of human sapovirus culture method]. [建立人类沙波病毒培养方法]。

Uirusu Pub Date : 2023-01-01 DOI: 10.2222/jsv.73.1

Hirotaka Takagi, Tomoichiro Oka

{"title":"[Establishment of human sapovirus culture method].","authors":"Hirotaka Takagi, Tomoichiro Oka","doi":"10.2222/jsv.73.1","DOIUrl":"https://doi.org/10.2222/jsv.73.1","url":null,"abstract":"More than 40 years after the discovery of human sapovirus (HuSaV), we have established a HuSaV culture system in which HuTu80 cells derived from the human duodenum adenocarcinoma cell line are cultured together with the addition of bile acid as a supplement. In addition to being a common cell line, this system using HuTu80 cells is a versatile method because classical culture media are available, and it is easy to scale-up for culture. However, the number of culture days required to obtain sufficient viral titer, the confirmation of viral gene conservation for sample selection, and the method for passaging of HuTu80-cells were crucial. So far, 15 genotypes have been successfully propagated and stocked, and stable supply as research resources has been achieved. Due to the above efforts, we can now proceed with the production and analysis of antisera using purified antigens and the evaluation of inactivation conditions. This manuscript introduces the background for selection of the cell line and bile acids, and the topics that have been discussed since the publication, as well as future issues that were raised such as the expression of cytopathicity and elucidation of low UV-C sensitivity of fecal-derived HuSaV.","PeriodicalId":75275,"journal":{"name":"Uirusu","volume":"73 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142334079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Structural insights into Hepatitis B and D virus entry receptors]. [乙型肝炎病毒和丁型肝炎病毒入口受体的结构研究]。

Uirusu Pub Date : 2023-01-01 DOI: 10.2222/jsv.73.89

Kanako Terakado Kimura, Koichi Watashi

引用次数: 0