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Translational biophotonics最新文献

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Photosensitizing proteins for antibacterial photodynamic inactivation 用于抗菌光动力失活的光敏蛋白
Translational biophotonics Pub Date : 2020-02-26 DOI: 10.1002/tbio.201900031
Cormac Hally, Pietro Delcanale, S. Nonell, C. Viappiani, S. Abbruzzetti
{"title":"Photosensitizing proteins for antibacterial photodynamic inactivation","authors":"Cormac Hally, Pietro Delcanale, S. Nonell, C. Viappiani, S. Abbruzzetti","doi":"10.1002/tbio.201900031","DOIUrl":"https://doi.org/10.1002/tbio.201900031","url":null,"abstract":"We review recent applications of water-soluble proteins as carriers for photosensitizing molecules in antimicrobial photodynamic therapy (aPDT). The low water solubility of photosensitizers and their tendency to aggregate often impair their photophysics, thus resulting in lower bioavailability of the compounds. Taking advantage of the spontaneous interaction between water soluble proteins endowed with hydrophobic cavities and the otherwise insoluble photosensitizing compounds, it is possible to obtain efficient delivery systems to be applied inaPDT. These self-assembled structures are endowed with good photosensitizing and fluorescence emission properties (comparable to those of monomerized photosensitizers) with high biocompatibility and warrant good bioavailability of the photoactive drug. The proteins used so far comprise serum albumins, β -lactoglobulin, and apomyoglobin, while photosensitizers considered up to now have been hypericin, curcumin, Zn-protoporphyrin IX, and Zn-phthalocyanine. Furthermore, mutants of fluorescent proteins derived from GFP-like systems or from flavin binding proteins complete the array of available photosensitizing proteins for aPDT.","PeriodicalId":75242,"journal":{"name":"Translational biophotonics","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/tbio.201900031","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42567029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 15
Translational Biophotonics : From research into clinical applications 转化生物光子学：从研究到临床应用
Translational biophotonics Pub Date : 2019-12-01 DOI: 10.1002/TBIO.201970001
J. Popp, C. Philipp, R. Sroka, C. Krafft, R. Hagen
{"title":"Translational Biophotonics\u0000 : From research into clinical applications","authors":"J. Popp, C. Philipp, R. Sroka, C. Krafft, R. Hagen","doi":"10.1002/TBIO.201970001","DOIUrl":"https://doi.org/10.1002/TBIO.201970001","url":null,"abstract":"","PeriodicalId":75242,"journal":{"name":"Translational biophotonics","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/TBIO.201970001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48085591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Fluorescence lifetime imaging for intraoperative cancer delineation in transoral robotic surgery. 荧光寿命成像用于经口机器人手术的术中癌症分界。
Translational biophotonics Pub Date : 2019-12-01 Epub Date: 2019-10-29 DOI: 10.1002/tbio.201900017
Brent W Weyers, Mark Marsden, Tianchen Sun, Julien Bec, Arnaud F Bewley, Regina F Gandour-Edwards, Michael G Moore, D Gregory Farwell, Laura Marcu
{"title":"Fluorescence lifetime imaging for intraoperative cancer delineation in transoral robotic surgery.","authors":"Brent W Weyers, Mark Marsden, Tianchen Sun, Julien Bec, Arnaud F Bewley, Regina F Gandour-Edwards, Michael G Moore, D Gregory Farwell, Laura Marcu","doi":"10.1002/tbio.201900017","DOIUrl":"10.1002/tbio.201900017","url":null,"abstract":"<p><p>This study evaluates the potential for fluorescence lifetime imaging (FLIm) to enhance intraoperative decisionmaking during robotic-assisted surgery of oropharyngeal cancer. Using a custom built FLIm instrument integrated with the da Vinci robotic surgical platform, we first demonstrate that cancer in epithelial tissue diagnosed by histopathology can be differentiated from surrounding healthy epithelial tissue imaged <i>in vivo</i> prior to cancer resection and <i>ex vivo</i> on the excised specimen. Second, we study the fluorescence properties of tissue imaged <i>in vivo</i> at surgical resection margins (tumor bed). Fluorescence lifetimes and spectral intensity ratios were calculated for three spectral channels, producing a set of six FLIm parameters. Current results from 10 patients undergoing TORS procedures demonstrate that healthy epithelium can be resolved from cancer (<i>P</i> < .001) for at least one FLIm parameter. We also showed that a multiparameter linear discriminant analysis approach provides superior discrimination to individual FLIm parameters for tissue imaged both <i>in vivo</i> and <i>ex vivo</i>. Overall, this study highlights the potential for FLIm to be developed into a diagnostic tool for clinical cancer applications of the oropharynx. This technique could help to circumvent the issues posed by the lack of tactile feedback associated with robotic surgical platforms to better enable cancer delineation.</p>","PeriodicalId":75242,"journal":{"name":"Translational biophotonics","volume":"1 1-2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7351319/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38152189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Issue Information 问题信息
Translational biophotonics Pub Date : 2019-12-01 DOI: 10.1002/tbio.201990001
{"title":"Issue Information","authors":"","doi":"10.1002/tbio.201990001","DOIUrl":"https://doi.org/10.1002/tbio.201990001","url":null,"abstract":"","PeriodicalId":75242,"journal":{"name":"Translational biophotonics","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/tbio.201990001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49060573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Advances in Doppler optical coherence tomography and angiography. 多普勒光学相干断层扫描和血管造影的研究进展。
Translational biophotonics Pub Date : 2019-12-01 Epub Date: 2019-11-20 DOI: 10.1002/tbio.201900005
Yan Li, Jason Chen, Zhongping Chen
{"title":"Advances in Doppler optical coherence tomography and angiography.","authors":"Yan Li, Jason Chen, Zhongping Chen","doi":"10.1002/tbio.201900005","DOIUrl":"10.1002/tbio.201900005","url":null,"abstract":"<p><p>Since the first demonstration of Doppler optical coherence tomography (OCT) in 1997, several functional extensions of Doppler OCT have been developed, including velocimetry, angiogram, and optical coherence elastography. These functional techniques have been widely used in research and clinical applications, particularly in ophthalmology. Here, we review the principles, representative methods, and applications of different Doppler OCT techniques, followed by discussion on the innovations, limitations, and future directions of each of these techniques.</p>","PeriodicalId":75242,"journal":{"name":"Translational biophotonics","volume":"1 1-2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/tbio.201900005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38447920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 18
Comparing in vivo and ex vivo fiberoptic diffuse reflectance spectroscopy in colorectal cancer 癌症体内和离体纤维光学漫反射光谱的比较
Translational biophotonics Pub Date : 2019-12-01 DOI: 10.1002/tbio.201900008
E. Baltussen, S. G. Brouwer de Koning, B. Hendriks, K. Jóźwiak, H. Sterenborg, T. Ruers
{"title":"Comparing in vivo and ex vivo fiberoptic diffuse reflectance spectroscopy in colorectal cancer","authors":"E. Baltussen, S. G. Brouwer de Koning, B. Hendriks, K. Jóźwiak, H. Sterenborg, T. Ruers","doi":"10.1002/tbio.201900008","DOIUrl":"https://doi.org/10.1002/tbio.201900008","url":null,"abstract":"In vivo data acquisition using fiberoptic diffuse reflectance spectroscopy (DRS) is more complicated and less controlled compared to ex vivo data acquisition. It would be of great benefit if classifiers for in vivo tissue discrimination based on DRS could be trained on data obtained ex vivo. In this study, in vivo and ex vivo DRS measurements are obtained during colorectal cancer surgery. A mixed model statistical analysis is used to examine the differences between the two datasets. Furthermore, classifiers are trained and tested using in vivo and ex vivo data. It is found that with a classifier trained on ex vivo data and tested on in vivo data, similar results are obtained compared to a classifier trained and tested on in vivo data. In conclusion, under the conditions used in this study, classifiers intended for in vivo tissue discrimination can be trained on ex vivo data.","PeriodicalId":75242,"journal":{"name":"Translational biophotonics","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/tbio.201900008","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44936290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
Front Cover 前盖
Translational biophotonics Pub Date : 2019-03-01 DOI: 10.1109/pria.2019.8786025
Author Not applicable
{"title":"Front Cover","authors":"Author Not applicable","doi":"10.1109/pria.2019.8786025","DOIUrl":"https://doi.org/10.1109/pria.2019.8786025","url":null,"abstract":"","PeriodicalId":75242,"journal":{"name":"Translational biophotonics","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48582448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A simple optofluidic platform for label-free cell-surface marker screening 一个简单的光流平台，用于无标记细胞表面标记筛选
Translational biophotonics Pub Date : 2014-06-17 DOI: 10.1117/12.2057806
Mustafa Mir, Olivia J. Scheideler, L. Sohn
{"title":"A simple optofluidic platform for label-free cell-surface marker screening","authors":"Mustafa Mir, Olivia J. Scheideler, L. Sohn","doi":"10.1117/12.2057806","DOIUrl":"https://doi.org/10.1117/12.2057806","url":null,"abstract":"Current technologies for cell surface marker screening such as flow cytometry and florescence microscopy, though indispensable, are not well suited for deployment in low resource or point-ofcare settings. Recently, node-pore sensing (NPS) has emerged as a microfluidic platform for labelfree cell surface marker screening. In NPS the transit time of individual cells being flowed through an antibody-functionalized microchannel are measured. Cells that express surface markers corresponding to a functionalized region are delayed due to specific, transient interactions with the surface. In this manner, the presence or absence of a particular surface marker is determined with single cell resolution. Here we show that by measuring the transit time optically as opposed to electrically, the abilities of NPS can be extended. We demonstrate this approach through measurements on human breast cancer cells. The technology presented here could potentially be deployed in low-resource settings as a diagnostic tool.","PeriodicalId":75242,"journal":{"name":"Translational biophotonics","volume":"24 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2014-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81721822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Depth sensitive oblique polarized reflectance spectroscopy of oral epithelial tissue 口腔上皮组织的深度敏感斜偏振反射光谱
Translational biophotonics Pub Date : 2014-05-19 DOI: 10.1117/12.2057584
M. Jiménez, S. Lam, Catherine F. Poh, K. Sokolov
{"title":"Depth sensitive oblique polarized reflectance spectroscopy of oral epithelial tissue","authors":"M. Jiménez, S. Lam, Catherine F. Poh, K. Sokolov","doi":"10.1117/12.2057584","DOIUrl":"https://doi.org/10.1117/12.2057584","url":null,"abstract":"Identifying depth-dependent alterations associated with epithelial cancerous lesions can be challenging in the oral cavity where variable epithelial thicknesses and troublesome keratin growths are prominent. Spectroscopic methods with enhanced depth resolution would immensely aid in isolating optical properties associated with malignant transformation. Combining multiple beveled fibers, oblique collection geometry, and polarization gating, oblique polarized reflectance spectroscopy (OPRS) achieves depth sensitive detection. We report promising results from a clinical trial of patients with oral lesions suspected of dysplasia or carcinoma demonstrating the potential of OPRS for the analysis of morphological and architectural changes in the context of multilayer, epithelial oral tissue.","PeriodicalId":75242,"journal":{"name":"Translational biophotonics","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2014-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85458710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Rapid multiplexed molecular phenotyping of ex vivo and in vivo tissues with targeted SERS NPs 体外和体内组织中靶向SERS NPs的快速多重分子表型分析
Translational biophotonics Pub Date : 2014-05-19 DOI: 10.1117/12.2057716
Y. Wang, Altaz Khan, Madhura Som, S. Y. Leigh, Danni Wang, Ye Chen, P. McVeigh, B. Wilson, Jonathan T. C. Liu
{"title":"Rapid multiplexed molecular phenotyping of ex vivo and in vivo tissues with targeted SERS NPs","authors":"Y. Wang, Altaz Khan, Madhura Som, S. Y. Leigh, Danni Wang, Ye Chen, P. McVeigh, B. Wilson, Jonathan T. C. Liu","doi":"10.1117/12.2057716","DOIUrl":"https://doi.org/10.1117/12.2057716","url":null,"abstract":"We are developing a miniature fiber-optic spectral-detection device and topical-staining protocol to rapidly detect multiplexed surface-enhanced Raman scattering (SERS) nanoparticles (NPs) targeted to cell-surface biomarkers in fresh tissues. Ex vivo and in vivo experiments were performed to optimize our strategy for the rapid detection of multiple cell-surface biomarkers following a brief (5 min) topical application of SERS NPs on tissues. The simultaneous detection and ratiometric quantification of targeted and nontargeted NPs allows for an unambiguous assessment of molecular expression that is insensitive to nonspecific variations in NP concentrations, potentially enabling point-of-care surgical guidance or early disease detection.","PeriodicalId":75242,"journal":{"name":"Translational biophotonics","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2014-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89360994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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